한라산 아고산대 구상나무림에서 연간 물질생산과 유기탄소량 변화1a

장래하 ( Rae Ha Jang ),조규태 ( Kyu Tae Cho ),유영한 ( Young Han You ) 한국환경생태학회 2014 한국환경생태학회지 Vol.28 No.6

국가장기생태사업(LTER)의 일환으로 한라산 아고산대 구상나무림의 물질생산과 탄소분포의 특성을 밝히고자 2009년부터 2013년까지 현존량, 유기탄소분포, 낙엽생산, 임상낙엽량과 토양 유기탄소 축척량을 조사하였다. 식물현존량은 상대생장법에 의해 보고된 물질생산 식을 이용하여 측정하고 이를 이산화탄소의 고정량으로 환산하였다. 2009, 2010, 2011, 2012 와 2013년의 현존량은 각각 98.88, 106.42, 107.67, 108.31 와 91.48 ton ha-1 였다. 이 기간 동안의 유기탄소는 지상부 생물량에 35.95, 38.69, 38.96, 39.46, 33.2 ton C ha-1, 지하부 생물량에 8.54, 9.2, 9.49, 9.28, 7.97 ton C ha-1 이 각각 분포하였다. 5년 동안 낙엽 생산을 통해 1.09, 1.80, 1.32, 2.46 와 1.20 ton C ha-1 의 유기탄소가 생태계로 유입되었다. 2010, 2011, 2012와 2013년의 임상낙엽층의 유기탄소량은 2.74, 2.43, 2.00 와 1.16 ton Cha-1였고, 토양 20cm깊이까지의 유기탄소 축적량은 55.77, 54.90, 50.69, 44.42 와 41.87 ton C ha-120cm-1였다. 이와 같이 현존량과 유기탄소량이 2009~2012년까지 매년 증가하였지만 태풍이 있었던 2013년에는 감소하였다. 이러한 현상은 자연적 교란이 한국의 아고산대 생태계에 크게 영향을 미친다는 것을 의미한다. Annual biomass production and amount of organic carbon in Abies koreana forest at Mt. Halla were conducted as a part of Korea National Long-Term Ecological Research (KNLTER). We measured standing biomass change of litter, soil production and organic carbon amounts of the forest floor and soil layer of A. koreana forest in Mt. Halla from 2009 to 2013 in permanent plots. Standing biomass, which was determined by allometric method, was converted into CO2. The standing biomass in A. koreana forest was 98.88, 106.42, 107.67, 108.31, 91.48 ton ha-1 in 2009, 2010, 2011, 2012 and 2013 year, respectively. The amount of annual carbon allocated to above ground was 35.95, 38.69, 38.96, 39.46, 33.2 ton C ha-1 and below ground biomass was 8.54, 9.2, 9.49, 9.28, 7.97 ton C ha-1 in 2009, 2010, 2011, 2012 and 2013 year, respectively. Amount of organic carbon returned to the forest via litterfall was 1.09, 1.80, 1.32, 2.46 and 1.20 ton C ha-1 in 2009, 2010, 2011, 2012 and 2013. Amount of organic carbon in annual litter layer on forest floor was 2.74, 2.43, 2.00 and 1.16 ton C ha-1 in 2010, 2011, 2012 and 2013 year, respectively. Amount of organic carbon within 20cm soil depth was 55.77, 54.9, 50.69, 44.42 and 41.87 ton C ha-120cm-1 in 2009, 2010, 2011, 2012 and 2013 year, respectively. Then standing biomass and organic carbon distribution increased steadily until 2012. But there declined in 2013 because of the typhoon Bolaven. Thus, standing biomass and organic carbon distribution of this subalpine forest were largely affected by natural disturbance factor.

Study on the Effect of Cimicifuga heracleifolia Ethanol Extract on Hyaluronic Acid Synthesis

( Hyun-kyu Son ),( Hun-yong Ha ) 한국공업화학회 2022 공업화학 Vol.33 No.6

Hyaluronic acid (HA) is a mucopolysaccharide, occurring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and skin aging. The purpose of this study was to examine the effects of Cimicifuga heracleifolia ethanol extract on the hyaluronic acid synthesis and the inhibition of hyaluronidase activity. To determine cytotoxicity, hyaluronic acid synthase 2 (HAS2) gene expression, HA production and, hyaluronidase inhibitory effects, 3-(4,5-dimethylthiazol-2-ly)-2,5-diphenyl tetrazolium bromide (MTT) assay, real time - polymerase chain reaction (RT-PCR), hyaluronic acid enzyme linked immunosorbent assay (HA-ELISA), and hyaluronidase assay were used, respectively. When the Cimicifuga heracleifolia extract was treated in the HaCaT cells up to 500 μg/mL concentration, cytotoxicity was confirmed by the Cimicifuga heracleifolia extract at concentrations above 200 μg/mL. Therefore, the optimum concentration of all experiments used in this study was determined to be 200 μg/mL. HAS2 gene expression increased by Cimicifuga heracleifolia extract in a concentration-dependent manner at all treatment concentrations. The production rate of HA was tended to decrease at the highest concentration of 200 μg/mL. The hyaluronidase activity inhibition effect of Cimicifuga heracleifolia extract was very high compared to the control group. Based on these results, Cimicifuga heracleifolia extract was expected to have a moisturizing effect on human skin and special attention should be paid to the determination of the concentration of Cimicifuga heracleifolia when developing cosmetic materials using it.

Budget and distribution of organic carbon in Taxus cuspidata forest in subalpine zone of Mt. Halla

Jang, Rae-Ha,Jeong, Heon-Mo,Lee, Eung-Pill,Cho, Kyu-Tae,You, Young-Han The Ecological Society of Korea 2017 Journal of Ecology and Environment Vol.41 No.1

Background: In order to investigate organic carbon distribution, carbon budget, and cycling of the subalpine forest, we studied biomass, organic carbon distribution, litter production, forest floor litter, accumulated soil organic carbon, and soil respiration in Taxus cuspidata forest in Halla National Park from February 2012 to November 2013. Biomass was calculated by using allometric equation and the value was converted to $CO_2$ stocks. Results: The amount of plant organic carbon was $13.60ton\;C\;ha^{-1}year^{-1}$ in 2012 and $14.29ton\;C\;ha^{-1}year^{-1}$ in 2013. And average organic carbon introduced to forest floor through litter production was $0.71ton\;C\;ha^{-1}year^{-1}$. Organic carbon distributed in forest floor litter layer was $0.73ton\;C\;ha^{-1}year^{-1}$ on average and accumulated organic carbon in soil was $51.13ton\;C\;ha^{-1}year^{-1}$ on average. In 2012, Amount of released $CO_2$ from soil to atmosphere was 10.93 ton $CO_2ha^{-1}year^{-1}$. Conclusions: The net ecosystem production based on the difference between net primary production of organic carbon and soil respiration was $-1.74ton\;C\;ha^{-1}year^{-1}$ releasing more carbon than it absorbed.

Sodium–Metal Halide and Sodium–Air Batteries

Ha ,, Seongmin,Kim ,, Jae‐,Kwang,Choi, Aram,Kim, Youngsik,Lee, Kyu Tae WILEY‐VCH Verlag 2014 ChemPhysChem Vol.15 No.10

<P>Impressive developments have been made in the past a few years toward the establishment of Na-ion batteries as next-generation energy-storage devices and replacements for Li-ion batteries. Na-based cells have attracted increasing attention owing to low production costs due to abundant sodium resources. However, applications of Na-ion batteries are limited to large-scale energy-storage systems because of their lower energy density compared to Li-ion batteries and their potential safety problems. Recently, Na-metal cells such as Na-metal halide and Na-air batteries have been considered to be promising for use in electric vehicles owing to good safety and high energy density, although less attention is focused on Na-metal cells than on Na-ion cells. This Minireview provides an overview of the fundamentals and recent progress in the fields of Na-metal halide and Na-air batteries, with the aim of providing a better understanding of new electrochemical systems.</P>

비글개에서 신원방우황청심원의 급성 및 아급성독성시험

성하정,권오경,방명주,곽형일,이진영,신대희,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs (3 males and 3 females) in acute experiments were administered orally with sinlge dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NSCH, 160 ㎎/㎏/day (low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NSCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NSCH in beagle dogs is considered to be safe.

비글개에서 신우황청심원의 급성 및 아급성독성시험

권오경,성하정,곽형일,방명주,신대희,이진영,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New Woohwangchungsimwon (NWCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs(3 males and 3 females) in acute experiments were administered orally with single dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NWCH, 160 ㎎/㎏/day(low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NWCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NWCH in beagle dogs is considered to be safe.

비글개에서 l-muscone의 급성독성 및 아급성독성시험 연구

유아선,권오경,성하정,곽형일,방명주,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of l-muscone, a major active ingredient of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs(3 males and 3 females) in acute experiments were administered orally with single dosage of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of l-muscone, 0.2㎎/㎏/day(low dosage group), 2 ㎎/㎏/day(middle dosage group), or 20 ㎎/㎏day(high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korean Food and Drug Administration(1996.4.16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ on oral route for both male and females. In animals administered with l-muscone, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, eye examination, hematology, serum chemistry, organ weight and other findings. No histolopathological lesions were observed in both control and treatment groups. Above data strongly suggest that l-muscone in beagle dogs is considered to be safe.

디젤엔진 代替燃料源으로서의 쉬나무의 造林學的인 特性에 關한 硏究

洪性珏,李麗夏,崔圭洪 建國大學校附設 農業資源開發硏究所 1983 論文集 Vol.8 No.-

쉬나무의 種實油는 디젤엔진의 代替燃料源으로서 그 가능성이 높은 것으로 알려지고 있는 바, 쉬나무의 栽培에 필요한 造林學的 特性을 알기 위하여, 本 硏究는 쉬나무의 自然分布地, 種實 및 木材生産性, 實生苗 및 接木苗 生産方法, 耐寒性度의 季節的 變異에 대해 調査하여, 다음과 같은 結果를 얻었다. 1. 推定年齡이 70∼150年生인 쉬나무들이 南部에서 中部에 이르기까지 넓게. 그러나 宮터, 古宮의 庭園, 農村의 마을 주변과 같이 제한된 지역에 自然相으로 分布하거나, 人工的으로 植栽되어 있는 것으로 판명되었다. 또한 쉬나무는 山麓部의 土深이 깊은 砂質壤土 및 植質壤土의 土壤에서 根萌芽에 의한 몇몇 群狀稚樹와 함께 孤立木으로 자라며, 母樹 주위에는 後代 實生稚樹의 發生이 드물었다. 쉬나무 實生稚樹 및 그 自然分布가 制限된 것은 自然狀態에서 多量의 種子가 野生鳥類의 먹이로 소비되어 버리고, 그 發芽率이 낮은 것이 주요 원인으로 생각된다. 2. 孤立木에서 樹冠面積(水平面積)에 대한 種字量을 推定했을 때, 年間 ha當 755 kg의 種字가 生産되고, 이로부터 264 kg의 種實油가 生産된다. 樹齡이 확인된 몇몇 나무의 경우, 20 年生 나무의 胸高直徑은 14 cm, 70 年生은 25∼26 cm였다. 樹高生長은 直徑生長과 상관없이 胸高直經 14∼83 cm의 나무의 경우 그 樹高는 6∼13 cm의 변이가 나타났다. 대부분의 大徑木이 主幹에 심한 心材腐朽를 일으켰는데, 그 原因이 凍害로 인한 것인지 또는 그외 다른 原因에 의한 것인지는 밝혀지지 않았다. 서울지방에 자라는 70 年生 나무에 있어서 主幹에는 心材腐朽가 있었으나, 14 年生 가지에는 心材腐朽가 없는 것으로 미루어 보아 지나간 14년간 材部柔組織에는 凍害가 없었음을 알 수 있었다. 木材는 전체적으로 보아 直線木理이나 觸單面에서는 放射柔組織을 중심으로 交叉木理를 갖고 木組織은 치밀하며, 氣乾比重은 2 年生에서 0.66, 70 年生에서 0.77이었다. 3. 種皮의 wax狀物質을 10% 合成洗劑 溶液으로 제거시킨 결과 發芽率이 10∼15%에서 80∼95%로 증가되었다. 70 年生 나무에서 얻은 接穗을 2 年生 台木에 切接으로 接木시킨 결과는 성공적이었다. 台木이 굵을수록 接穗의 生長이 좋았다. 4. 種字産地別로 幼苗의 耐寒性度에는 차이가 나타나지 않았다. 이와 같이 耐寒性度의 차이가 적은 것은 이 樹種이 蟲媒花이지만 암꽃과 수꽃의 꽃피는 시기가 다르므로 他家受精이 가능하여 집단간에 遺傳子交換이 될 수 있기 때문인 것으로 解析되었다. 1 年生 苗木은 2 年生 苗木보다 耐寒性度가 더 낮은데 이는 1 年生 苗木의 생장기간이 보다 길기 때문에 材部柔組織의 소수의 細胞가 耐寒性 發達이 늦기 때문에 나타난 것으로 생각되었다. 1 年生 苗木의 凍害防止를 위해서는 겨울동안 땅속에 假植을 하는 것이 가장 좋은 방법이다. To know the silvicultural characteristics of Evodia daniellii, of which the seed oil was known to have the high feasibility of fuel substitution for diesel engine, this study investigated the natural distribution of the tree, the productivity of the seed and wood in the natural condition, the production of the seedlings from the seeds and the grafts, and the seasonal variation in cold hardiness of the seedlings from different provenance. The following results were discussed; 1. It appeared that Evodia daniellii, estimated as old as 70-200 years, are naturally distributed and/or artificially planted widely from the southern part up to the middle part of Korea, but in a limited spot such as the site of an ancient castle, the garden of palace and near rural village, and that the trees are growing as an isolated tree with a few progeny near the mother tree, at the site of good soil depth with sandy loam in the lower part of hillside. The limited natural distribution of this species was attributed to large consumption of the seed by feeding the wild birds and low percent germination in the natural condition. 2. The seed produced annually, as estimated by the amount of seed in an isolated tree per the area projected by the crown, averaged 755kg/ha, resulting in 264kg/ha of the seed oil. In some trees of which the age was able to be identified, the diameter growth at the age of 20 years was 14 cm, and 25 to 26 cm at the age of 70 years. The heigh growth was not consistent with the diameter growth or the age and varied from 6 to 13 meter in the trees of diameter 14 to 83 cm. The main stem of most of large diameter trees showed various heart rot damage of which causes were not clear whether it was brought about by freezing or other incidents. In one 70 years-old tree growing in the northern limit of Korea with serious heart rot in the main stem, the 14 year old branch showed no sign of the heart rot, indicating that there has been no freezing injury on the main branch for 14years. The wood had straight grain over all, otherwise interlocked grain around the ray structure on the tangential section, dense texture, specific gravity (air dried) of 0.66 in two year old stem and of 0.77 in 70 year old stem. 3. Removal of wax like material from the surface of the seed coat with 10% solution of synthetic soap promoted the percent germination from 10-15% to 80-95%. The veneer grafting of the scion from the 70 year old tree to 1 to 2 year old stock was successful. The thicker the stock was used, the better the growth of scion was obtained. 4. There was little difference in cold hardiness among seedlings from different provenance. The little difference could be attributed to the out-breeding mechanism, even though this species is pollinated by insects, in that earlier flowering of the male flower than the female one may allow the gene exchange among the population. One year old seedling was less cold hardy than the two year old one. This might be attributed to the slow development in cold hardiness of the small group of cells in the xylem, which was probably resulted from longer growth period of one year old seedling than the older trees. The cold damage of one year old seedlings could be prevented by transplanting them in the soil temporarily during winter

Budget and distribution of organic carbon in Quercus serrata Thunb. ex Murray forest in Mt. Worak

Lee, Seung-Hyuk,Jang, Rae-Ha,Cho, Kyu-Tae,You, Young-Han The Ecological Society of Korea 2015 Journal of Ecology and Environment Vol.38 No.4

The carbon cycle came into the spotlight due to the climate change and forests are well-known for their capacity to store carbon amongst other terrestrial ecosystems. The annual organic carbon of litter production, forest floor litter layer, soil, aboveground and belowground part of plant, standing biomass, net primary production, uptake of organic carbon, soil respiration, etc. were measured in Mt. Worak in order to understand the production and carbon budget of Quercus serrata forest that are widely spread in the central and southern part of the Korean Peninsula. The total amount of organic carbon of Q. serrata forest during the study period (2010-2013) was 130.745 ton C ha^-1. The aboveground part of plant, belowground part of plant, forest floor litter layer, and organic carbon in soil was 50.041, 12.510, 4.075, and 64.119 ton C ha^-1, respectively. The total average of carbon fixation in plants from photosynthesis was 4.935 ton C ha^-1 yr^-1 and organic carbon released from soil respiration to microbial respiration was 3.972 ton C ha^-1 yr^-1. As a result, the net ecosystem production of Q. serrata forest estimated from carbon fixation and soil respiration was 0.963 ton C ha^-1 yr^-1. Therefore, it seems that Q. serrata forest can act as a sink that absorbs carbon from the atmosphere. The carbon uptake of Q. serrata forest was highest in stem of the plant and the research site had young forest which had many trees with small diameter at breast height (DBH). Consequentially, it seems that active matter production and vigorous carbon dioxide assimilation occurred in Q. serrata forest and these results have proven to be effective for Q. serrata forest to play a role as carbon storage and NEP.

분포형 토양침식지도를 이용한 한강상류지역 토양유실 위험성 평가

박찬원(Chan-Won Park),손연규(Yeon-Kyu Sonn),장용선(Yong-Seon Zhang),홍석영(S. Young Hong),현병근(Byung-Keun Hyun),송관철(Kwan-Cheol Song),하상건(Sang-Keun Ha),문용희(Young-Hee Moon) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.6

본 연구에서는 1:25,000 정밀토양도 등 가용한 공간자료를 이용하여 작성된 토양유실도를 이용하여 한강 상류지역 10개 중권역 108 소유역에 대하여 토양유실량 평가하고 토지이용별 토양유실 위험지역을 분석하였다. 대상유역 총 토양유실량은 895만 Mg yr<SUP>-1</SUP>으로 예측되었으며, 면적당 평균 토양유실량은 6.1 Mg ha<SUP>-1</SUP>yr<SUP>-1</SUP>이었다. 중소유역 단위로 살펴보면 남한강지역이 북한강지역의 유실량 뿐만 아니라 면적당 유실량이 더 많았다. 이는 북한강 권역의 경우 화강암 및 화강편마암이 주요 모재인 반면에 남한강 권역의 경우 토양모재가 퇴적암지대를 많이 포함있어 토양침식성 인자 (K factor)와 경사 인자 (LS factor)가 상대적으로 높고, 남한강 지역의 경우 농경지 토양이 상대적으로 많이 분포하고 있기 때문으로 판단된다. 대상유역의 토지이용별 연평균 토양유실량을 분석한 결과 산림/초지 > 밭 >> 도심/대지 >> 논 > 과수의 순로 분석 대상유역 중 10.7%를 차지하고 있는 농경지에서 유실되는 토양의 추정량은 41.3%이였으며, 이중 6.2%면적을 차지하는 밭이 40.6%으로 44.2%의 산림/초지와 비슷한 수준이었다. 한강상류지역 토양유실 위험성 평가시 분포형 토양침식지도와 항공사진을 비교한 결과 토양유실에 대한 공간인 정보를 확실하게 보여주고 있으며, 토양침식위험성은 남한강권역의 남한강 상류 (1001), 평창강 (1002), 충주댐 (1003) 권역에서 각각 토양침식위험성이 “보통”이상으로 높은 지역이 각각 8.7%, 7.9%, 7.8%로 평균인 5.9%보다 높았으며, 이에 대한 보전방안이 강구될 필요가 있고 판단된다. This study was conducted to evaluate soil erosion risk with a standard unit watershed in the upper Han river basin using the spatial soil erosion map according to the change of landuse. The study area is 14,577 ㎢, which consists of 10 subbasins, 107 standard unit watersheds. Total annual soil loss and soil loss per area estimated were 895 × 10<SUP>4</SUP> Mg yr<SUP>-1</SUP> and 6.1 Mg ha<SUP>-1</SUP> yr<SUP>-1</SUP>, respectively. A result of analysis with a subbasin as a unit showed that annual soil losses and soil loss per area in Namhan river basins was more than in Bukhan river ones. Predicted annual soil loss according to the landuse ranked as Forest & Grassland > Upland >> Urban & Fallow area > Paddy field > Orchard. Upland area covered 6.2% of the study area, but the contribution of total annul soil loss was 40.6% and that of Forest & Grassland was 44.2%. As a evaluation of soil erosion risk using the spatial soil erosion map, we could precisely conformed the potential hazardous region of soil erosion in each unit watersheds. The ratio of regions, graded as higher “Moderate” for annual soil loss, were respectively 8.7%, 7.9% and 7.8% in 1001, 1002 and 1003 subbasins in Namhan river basin. Most landuse of these area was upland, and these area is necessary to establish soil conservation practices to reduce soil erosion based on the field observation.