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      • KCI우수등재

        반축가축영양;호맥건초의 생육단계와 절단이 산양의 질소 및 Energy Balance 에 미치는 영향

        전병태,문상호,광전수헌 한국축산학회 1995 한국축산학회지 Vol.37 No.1

        This experiment was carried out to study associative effects of the chopping of hay at two stages of maturity on the nitrogen balance, nutritive value and energy balance in goats fed rye hay. Twelve Japanese goats were fed rye hay ad libitum in a unchopped form or chopped to 2 cm of chopping lengths. They were grouped into tetrads as follows: B I and B II were fed with unchopped and chopped hay at the boot stage, H I and H II were led unchopped and chopped hay at the heading stage respectively. Nitrogen intake(NI) and digestible nitrogen (DN) were significantly higher(p$lt;0.05) in groups (B I and B II) fed hay at boot stage ttran in groups(H I and H II) fed hay at heading stage. However, the differences corresponding with the chopping of hay were not accepted at both growth stages. Nitrogen losses as feces(FN) and urine(UN) were similar to the results for NI and DN, RN was high in groups(B I and B II) fed hay at the boot stage, although there was no significant difference (p$gt;0.05), but that had little differences in relation to the chopping of hay at both growth stages. On the other hand, the ratio of FN to NI wars higher in H I and H II than in B I and B II, while there were no great differences(p$gt;0.05) between unchopped hay and chopped hay at both growth stages. The ratio of UN to NI was similar of both growth stages. GE in groups(H I and H II) fed hay at the heading stage were significantly higher(p$gt;0.05) than those in groups fed hay at the boot stage. However, DE and ME were greater in groups(B I and B II) fed hay at the boot stage, although there was no significant difference for ME. TDN and DCP were also significantly higher(p$gt;0.05) in B I and B II than in H I and H II. No differences were recognized corresponding to the chopping of hay at both stages for GE, DE, ME, TDN and DCP. EI was a little lower in H I and H II than in B I and B II but was not significant. Energy lasses in FE, UE, CH, and HP did not differ in relation to the chopping and growth stage of hay. RE was 148.9, 108.9, 23.2 and 51.9 MJ/㎏W^(0.75) for B I, B II, H I and H II respectively, and thus RE in groups(B I and B II) fed hay at boot stage were significantly larger(p$lt;0.05) than those in groups(H I and H II) fed hay at heading stage. But there were no effects of the chopping of hay at both growth stages. RE was closely related with dry matter and energy intake(p$lt;0.05), especially with digestible dry matter and digestible energy intake(p$lt;0.01).

      • SCOPUSKCI등재

        폐암세포주의 TNF-$\alpha$ 유발 apoptosis에서 NF-${\kappa}B$의 역할

        김재열,이승희,황보빈,이춘택,김영환,한성구,심영수,유철규,Kim,,J.Y.,Lee,,S.H.,HwangBo,,B.,Lee,,C.T.,Kim,,O.H.,Han,,S.K.,Shim,,O.S.,Yoo,,C.G. 대한결핵및호흡기학회 2000 Tuberculosis and Respiratory Diseases Vol.48 No.2

        연구배경: 현재 사용되고 있는 많은 항암제는 암세포의 apoptosis를 유도함으로서 암세포를 사멸시키는 것이 주된 작용 기전이다. 항암제에 의한 치료 실패의 주된 원인은 암세포의 apoptosis에 대한 내성 획득에 의한 것으로 이해되고 있다. 최근의 보고에 의하면 많은 암세포주에서 apoptosis에 대한 내성 획득에 NF-${\kappa}B$의 활성화가 중요한 역할을 한다고 알려져 있지만 일부 세포에서는 상반된 결과를 보이고 있어 apoptosis에 대한 내성 획득과 NF-${\kappa}B$의 활성화와의 관련성은 세포에 따라 차이를 보이고 있다. 본 연구에서는 비소세포폐암 세포주가 TNF-$\alpha$ 유발 apoptosis에 저항을 나타내는 기전에서 NF-${\kappa}B$의 역할을 규명하고자 하였다. 방 법: 비소세포폐암세포주인 NCI-H157 세포에 TNF-$\alpha$ cycloheximide(CHX), TNF-$\alpha$와 CHX를 각각 투여하고 24시간 후 MTT assay로 세포생존율을 평가하였고 apoptosis의 발생 유무는 PARP에 대한 Westrn 분석으로 평가하였다. Ad5LacZ와 $Ad5I{\kappa}B{\alpha}SR$를 20시간 동안 감염시킨 각각의 세포를 1, 5, 10, 20, 50 ng/ml의 TNF-$\alpha$로 24, 48시간 자극 후 MTT assay를 시행하였고, 같은 농도의 TNF-$\alpha$로 24, 48시간 자극 후 PARP에 대한 Western 분석을 시행하였다. $I{\kappa}B{\alpha}$의 분해를 억제하는 proteasome inhibitor 인 MG132를 전처치하고 TNF-$\alpha$로 24, 48 시간 자극 후 MTT assay와 PARP에 대한 Westrn 분석을 시행하였다. $Ad5I{\kappa}B{\alpha}SR$를 감염시킨 세포를 TNF-$\alpha$로 자극한 후 NF-${\kappa}B$의 활성화를 EMSA로 평가하였다. 결 과: 1. TNF-$\alpha$ 단독 투여로는 세포 생존율의 감소와 apoptosis가 관찰되지 않았고 TNF-$\alpha$와 CHX를 같이 투여하였을 때는 세포 생존율의 감소와 함께 apoptosis가 유도되었다. 2. $Ad5I{\kappa}B{\alpha}SR$의 감염으로 $I{\kappa}B{\alpha}$가 과발현된 세포와 MG132를 전처치한 세포에서 TNF-$\alpha$ 자극에 의한 NF-${\kappa}B$의 활성화가 억제되었다. 3. $Ad5I{\kappa}B{\alpha}$ SR를 감염시킨 세포와 MG132를 전처치하여 NF-${\kappa}B$의 활성을 억제한 세포에서 TNF-$\alpha$ 자극 후 세포 생존율이 감소하고 apoptosis가 유도되었다. 결 론: 비소세포폐암 세포주의 TNF-$\alpha$ 유발 apoptosis에 대한 내성은 NF-${\kappa}B$의 활성화에 의해 생성되는 새로운 단백의 발현에 의한 것으로 생각되며, NF-${\kappa}B$ 활성화의 억제로 이를 극복할 수 있을 것으로 사료된다. Background: The main reason for the failure of anti-cancer chemotherapy is the build up of resistance by cancer cells to apoptosis. The activation of NF-${\kappa}B$ in many cancer cell lines is reported to be underlying mechanism behind the build up of resistance of cancer cells to apoptosis. However, this relationship varied depending on the cells used in the experiments. In this study, the role of NF-${\kappa}B$ activation in the TNF-$\alpha$-induced apoptosis in lung cancer cell line was evaluated. Methods: NCI-H157 cells were used in all experiments. Cells were exposed to a high dose of TNF-$\alpha$(20 ng/ml) for 24 or 48 hours with or without blocking NF-${\kappa}B$ activation. TNF-$\alpha$-induced activation of NF-${\kappa}B$ was inhibited either by overexpression of $I{\kappa}B{\alpha}$-super repressor($I{\kappa}B{\alpha}$-SR) or by pre-treatment with proteasome inhibitor. Cell viability and apoptosis were evaluated with MTT assay and Western blot analysis for PARP fragment, respectively. Results: Cell viability of NCI-H157 cells was not affected by TNF-$\alpha$ treatment alone; however, combined treatment with TNF-$\alpha$ and cycloheximide reduced cell viability significantly, indicating that resistance to TNF-$\alpha$ is mediated by the new proteins synthesized after TNF-$\alpha$ stimulation. To evaluate the role of NF-${\kappa}B$ in the transcription of anti-apoptotic proteins. delete NF-${\kappa}B$ activation was inhibited before TNF-$\alpha$ stimulation. as described above. $AD5I{\kappa}B{\alpha}$-SR-transduction inhibited TNF-$\alpha$-induced nuclear translocation of p65. TNF-$\alpha$-induced cell death and apoptosis increased after inhibition of TNF-$\alpha$-induced activation of NF-${\kappa}$ by methods. Conclusion: These results suggest that TNF-$\alpha$-induced activation of NF-${\kappa}B$ may be closely related to the acquisition of the resistance to TNF-$\alpha$-induced apoptosis in lung cancer cells. Therefore. blocking of NF-${\kappa}B$ pathway can be a useful therapeutic modality in the treatment of lung cancer.

      • KCI등재

        돼지 H-FABP 유전자의 다형성 및 경제 형질과의 연관성 구명

        최봉환,김태헌,이지웅,조용민,이혜영,조병욱,정일정 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5

        The purpose of this study was to detect association between genetic variation and economic trait in the porcine heart type fatty acid-binding protein gene as a candidate gene for the traits related with growth and meat quality in pigs. The H-FABP is a 15-kDa protein expressed in several tissues with high demand for fat metabolism such as cardiac and skeletal muscle and lactating mammary gland. H-FABP is small intracellular protein involved in fatty acid transport from the plasma membrane to the site of β-oxidation and/or triacylglycerol or phospholipid synthesis. In this study, H-FABP PCR-RFLP was performed in F_(2) population composed of 214 individuals form an intercross between Korean Native Boars and Landrace sows. PCR products form tow primer sets within H-FABP gene were amplified in 850bp and 700bp. Digestion of PCR products with the restriction digestion enzymes HaeⅢ and Hinf Ⅰ, revealed fragment length polymorphisms(RFL. Ps). The genotype frequencies from H-FABP/HaeⅢ was .29 for genotype DD, .53 for genotype Dd, and .15 for genotype dd, respectively. The genotype frequencies of HH, Hh, and hh from H-FABP(hinf Ⅰ was .38, .41, and .20, respectively, in the population.Relationships between their genotypes and economic traits were estimated. In H-FABP/HaeⅢ locus, there were specific genotypes(Dd and dd) associated with economic traits such as body weight. In H-FABP/Hinf Ⅰ Iocus, Genotypes of HH and Hh associated with growth traits such as body weights at 5, 12, and 30 week of age (p<.05 or p<.001) and back fat thickness, body fat including abdominal and trimmed fat (p<.001) and intramuscular fat(p<.05). The 'H'allele was positivecly associated with gaining of body weight and fatness deposition. In conclusion, a significant association of the H-FABP gene from its genetic variation was found on body weight, intramuscular fat and backfat thickness.

      • KCI등재

        Nd₂Fe₁₄B 희토류 영구자석의 전자기적 물성연구

        민병일(B. I. Min), 정윤희(Y. H. Jeong), 양충진(C. J. Yang) 한국자기학회 1992 韓國磁氣學會誌 Vol.2 No.3

        희토류 영구자석, Nd₂Fe₁₄B 화합물에 대한 자체충족적 국재밀도함수근사 전자 구조 계산을 수행하여 이 물질의 전자기적 물성을 연구하였다. LMTO(Linearized Muffin-Tin Orbital)에너지 띠 방법을 사용하여 상자성, 강자성상에서 구한 Nd₂Fe₁₄B 화합물의 에너지 띠구조를 토대로 하여 자성을 포함한 제반 물성, 즉 희토류금속과 천이금속의 결합(bonding)효과, 전기적, 자기적 구조등을 고찰하였다. Boron원자의 역학은 근접 Fe 원자와의 혼합 상호작용을 통하여 Fe의 원자의 자기모멘트를 많이 줄이는 효과를 주며 또한 구조 안정성에 기여한다는 결과를 얻었다. 강자성상에서의 Fe 원자들의 평균 자기모멘트는 약 2.15 μ_B로 계산되었는데 이중 Boron 원자로부터 가장 멀리 떨어져 있으며 12개의 Fe 원자들로 둘러싸인 Fe (j2-site)원자가 가장 큰 값(2.7 μ_B)의 자기모멘트를 갖고 Boron 원자와의 혼합 상호작용이 가장 큰 Fe(e-site)원자가 가장 작은 값(1.9 μ_B)의 자기모멘트를 갖는다. Electronic and magnetic propertis of the rare-earth permanent magnet, Nd₂Fe₁₄B, are investigated by performing self-consistent local density functional electronic structure calculations. Employing the LMTO (linearized muffin-tin orbital) band method, we have obtained the electronic band structures for both paramagnetic and ferromagnetic phases of Nd₂Fe₁₄B. Based on the energy band structures, we have studied various physical properties, such as electronic and magnetice structures, we have studied various physical properties, such as electronic and magnetic structures and the bonding effect between the rare-earth and transition metals. It is found that the boron atom substantially reduces the magnetic moment of neighboring Fe atoms through the hybridization interaction and that it plays an important role in stabilizing the structure. The average magnetic moment of Fe atoms in the ferromagnetic phase is estimated to be 2.15 μ_B. The Fe atom in the j2-site, which is located farthest from the B atom and surrounded by 12 Fe nearest neighbors, has the biggest magnetic moment (2.7 μ_B), while the Fe in the e-site, which interacts most strongly with B atoms, has the smallest magnetic moment (1.9 μ_B).

      • SCIESCOPUSKCI등재

        Cloning and Expression of Lactate Dehydrogenase H Chain Gene in Adipose Tissues of Korean Cattle

        Kim,,H.H.,Seol,,M.B.,Jeon,,D.H.,Sun,,S.S.,Kim,,K.H.,Choi,,Y.J.,Baik,,M.G. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.12

        To understand molecular mechanisms that regulate deposition and release of intramuscular fat, a fasting-induced clone was identified by differential screening from cDNA library of adipose tissues of Korean cattle. The clone had a total length of 1,319 nucleotides coding for 334 amino acids. It was identified as one encoding L-lactate dehydrogenase H chain (LDH-B). Comparison of the deduced amino acid sequences of bovine LDH-B with those of pig, human, rat, and mouse showed 98%, 98%, 97%, and 96% identity, respectively. Food deprivation for 48 h increased mRNA levels of LDH-B gene in adipose tissues of Korean cattle compared to fed- and 6 h refed- tissues. The expression of obese mRNA was examined for individual adipose tissue from several fat depots. Fasting induced expression of LDH-B gene in subcutaneous adipose tissues, but it did not affect expression levels in abdominal, perirenal and intramuscular tissues. Results demonstrate that induction of LDH-B gene during fasting may represent a metabolic shift from anaerobic state to aerobic predominance in fasted adipose tissues and that its responses to fasting are different among several adipose tissues.

      • SCIESCOPUSKCI등재

        A Timetable of the Early Development Stage of Silkies Embryo

        Li,,B.C.,Chen,,G.H.,Qin,,J.,Wang,,K.H.,Xiao,,X.J.,Xie,,K.Z.,Wu,,X.S. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.6

        The early embryos are obtained in different time after the former egg had been laid, and the aim of the present study was to observe the development law of chicken early embryo.The embryo development has been divided into the two periods according to morphology of blastodisc. Cleavage period, from 5.5 h (0 h uterine age) to 15.5 h (10-10.5 h uterine age) after the former egg had laid, formation blastodisc of 6-7 layers cell. Later blastocyst period, from 17.5 h (12-12.5 h uterine age) to area pellucida formation after the former egg had been laid. The first division took place at 5 h (0 h uterine age), morular at 11.5 h (6-6.5 h uterine age), and blastocyst at 15.5 h (10-10.5 h uterine age) after the former egg had been laid.

      • KCI등재후보SCOPUS

        Effects of Dietary Supplementation with Hainanmycin on Protein Degradation and Populations of Ammonia-producing Bacteria In vitro

        Z.B.,Wang,H.S.,Xin,M.J.,Wang,Z.Y.,Li,Y.L.,Qu,S.J.,Miao,Y.G.,Zhang 아세아·태평양축산학회 2013 Animal Bioscience Vol.26 No.5

        An in vitro fermentation was conducted to determine the effects of hainanmycin on protein degradation and populations of ammonia-producing bacteria. The substrates (DM basis) for in vitro fermentation consisted of alfalfa hay (31.7%), Chinese wild rye grass hay (28.3%), ground corn grain (24.5%), soybean meal (15.5%) with a forage: concentrate of 60:40. Treatments were the control (no additive) and hainanmycin supplemented at 0.1 (H0.1), 1 (H1), 10 (H10), and 100 mg/kg (H100) of the substrates. After 24 h of fermentation, the highest addition level of hainanmycin decreased total VFA concentration and increased the final pH. The high addition level of hainanmycin (H1, H10, and H100) reduced (p<0.05) branched-chain VFA concentration, the molar proportion of acetate and butyrate, and ratio of acetate to propionate; and increased the molar proportion of propionate, except that for H1 the in molar proportion of acetate and isobutyrate was not changed (p>0.05). After 24 h of fermentation, H10 and H100 increased (p<0.05) concentrations of peptide nitrogen and AA nitrogen and proteinase activity, and decreased (p<0.05) NH3-N concentration and deaminase activity compared with control. Peptidase activitives were not affected by hainanmycin. Hainanmycin supplementation only inhibited the growth of Butyrivibrio fibrisolvens, which is one of the species of low deaminative activity. Hainanmycin supplementation also decreased (p<0.05) relative population sizes of hyper-ammonia-producing species, except for H0.1 on Clostridium aminophilum. It was concluded that dietary supplementation with hainanmycin could improve ruminal fermentation and modify protein degradation by changing population size of ammonia-producing bacteria in vitro; and the addition level of 10 mg/kg appeared to achieve the best results.

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