Parthenogenetic Activity of Porcine Sperm Factor to In Vìtro Matured tured Porcine Oocyte

Park, Chun_Gyu,Jin-Ki Parκ,Sung-Woo Kim,Ju-Young Lee,Joo-hee Han,Seung-Eun Lee,Kyung-Nye Baek,Won-Chung Chang 한국동물생명공학회(구 한국동물번식학회) 2004 Reproductive & Developmental Biology(Supplement) Vol.28 No.2s

식중독균 항원(Salmonella typhimurium)에 의한 계란항체(IgY) 생산성과 항체특성 및 항균효과

백경연,손규목,배만종,백반석,한준표 경산대학교 생명자원개발연구소 1999 생명자원과 산업 Vol.3 No.-

본 연구는 식중독균을 항원으로 하여 산란계에 면역시킨 후 계란 항체(IgY)의 생산 가능성을 검토하고, 그 항체의 특성과 항균성을 조사하고자 실시하였다. 그 방법으로는 항원으로 사용된 Salmonella typhimurium(ATCC 14028) 등을 incomplete Freund's adjuvant와 동량으로 혼합하여 면역시킨 후 10주간 생산된 계란을 수집하여, 계란항체(IgY)의 분리 및 식중독균 항원에 대한 항체의 특성, 항균성, 생화학적 특성을 측정하였다. S. typhimurium 항원에 면역된 산란계의 계란항체(IgY)생산 및 증가를 측정한 결과, 산란계가 이들 항원에 면역반응을 일으켜 2주 후 혈청과 난황에서 각각 normal군에 비하여 뚜렷한 항체가 생성능을 보였으며, 혈청내의 IgG생성에 비하여 계란항체(IgY)의 생성이 다소 낮은 것으로 나타났다. 혈청 및 난황내의 단백질 함량은 normol군에 비하여 약간 높은 경향을 나타내어 산란계의 혈청 및 난황내의 단백질이 이들 항원에 의하여 증가하는 것으로 나타났다. DEAE-Sephacel column을 통과한 fraction은 두 개의 peak를 나타내었다. 각 fraction의 IgY농도를 ELISA법으로 확인한 결과 두 번째 peak에서 대부분 측정되었다. 분리 정제된 IgY의 분자량을 SDS-polyacrylamide 전기영동법으로 측정한 결과, heavy chain 72∼75KD, light chain 30∼40KD정도였다. Anti-S. typhimurium 항체의 온도에 대한 활성은 60℃에서 30분 가열시 91.5%, 65℃에서 15분 가열시 각각 73.2%의 활성도를 나타내었으며, 80℃에서 15분간 가열시는 거의 활성이 없는 것으로 나타났다. pH에 대한 안정성은 pH 7부터 pH 4까지는각각 98.7%의 활성도를 유지하다가 pH 3부터 급격히 감소하여, pH 2에서는 9.6%로 거의 활성도를 나타내지 않았다. 항균활성을 응집가, 생균수, disc diffusion susceptibility test 등으로 측정한 결과 anti-S. typhimurium 항체의 항균활성도는 매우 미약하게 나타났다. 이상의 결과에서, 본 실험에서 사용된 IgY는 항원에 대한 특이성 및 생화학적 특성 등을 고려해 볼 때 앞으로 식중독균 제거 및 예방 치료뿐만 아니라 식품산업 소재, 각종 질병예방을 위한 의약품 소재, 연구용 kit와 진단용 kit 및 축산분야 등 여러 분야에 응용될 수 있으리라 기대된다. This study was carried out to investigated to get a industrial information from discussion about productive a possibility if IgY antibody, antimicrobial activity and properties of IgY antibody in egg yolk. Immunoglobulin Y in egg yolk and immunoglobulin G in serum were obtained from gens which were immunized with Salmonella typhimurium(ATCC14028). These antibody titers were evaluated in serum and egg yolk by ELISA. After the initial immunization the anti-Salmonella typhimurium IgY antibody level gradually were decreased from firth week to tenth week. On the other hand, the antibody level in the serum were increased from the first week, reaching its peak in the sixth week. The residual antibody activities of IgY were 91.5% after heating for 30min at 60℃. At the same time, were 73.2% after heating 15min at 65℃ and decreased vapidly at 70℃ and little antibody activity was left after heating for 15min at 80℃. When the prepared IgY was stable from pH 7 to 4 and remained to 69.8% at pH 3, respectively.

Factitious Fever로 밝혀진 불명열 1예

김백남,강재명,배인규,김양수,우준희,류지소 대한감염학회 2000 감염 Vol.32 No.5

2009년 인플루엔자 대유행 중 인플루엔자 진료소 방문자의 연령 분포 변화

김백남,곽이경,문치숙,김연숙,김의석,배인규,염준섭,이창섭,허지안 대한감염학회 2010 감염과 화학요법 Vol.42 No.2

The pandemic influenza (H1N1 2009) virus, after being introduced in Korea in April, 2009, spread rapidly nationwide in mid-2009. This study was conducted to characterize trend in age distribution of visitors to Flu-clinics during the pandemic. Demographic, clinical and laboratory data of visitors to flu clinic from Week 36 to 52 (August 30 to December 26) of 2009 were retrieved and collected from electronic databases at 9 hospitals. Visitors 0-6, 7-12, 13-18, 19-29, 30-64, and 65 years or more of age were classified into group I to VI, respectively. A total of 107,467 visitors were seen at Flu-clinics for a 17-week study period. Of those, 32,485 were laboratory-confirmed. Antivirals were prescribed for 62,533 visitors. Numbers of visits, prescriptions of antivirals, and laboratory-confirmed cases of the pandemic influenza (H1N1 2009) peaked at Week 44. A large number of visits by group II and III were followed by those of group I and V, especially around the peak. Numbers of visits by group VI were lowest in all hospitals. In some hospitals, higher number of visits lasted longer in children than in adults while vice versa in other hospitals depending on the location. In summary, the pandemic influenza (H1N1 2009) was presumed to peak in late October and involved majorly children and students in Korea. Unique age distribution of visitors to flu clinic was observed in some hospitals.

전류형 능동 전력필터를 위한 델터변조기법에 관한 연구

최규하,백형래,김한성 建國大學校 附設 産業技術硏究所 1992 논문집 Vol.17 No.-

Delta modulation technique for current-type active power filter is proposed which can not only eliminate the harmonic current but compensate the reactive power at the AC sides where the nonlinear loads are connected. The delta modulated current control can be used to improve both the response and the control capability of the injected compensating current for the active filter with constant current source. As a result the compensated ac line current becomes nearly sinusoidal and also the input power factor is improved roughly to unity.

Graphene: Position‐ and Morphology‐Controlled ZnO Nanostructures Grown on Graphene Layers (Adv. Mater. 41/2012)

Kim, Yong‐,Jin,Yoo, Hyobin,Lee, Chul‐,Ho,Park, Jun Beom,Baek, Hyeonjun,Kim, Miyoung,Yi, Gyu‐,Chul WILEY‐VCH Verlag 2012 Advanced Materials Vol.24 No.41

<P>On page 5565, Gyu‐Chul Yi and co‐workers grow position‐ and morphology‐controlled ZnO nanowalls in prescribed positions on graphene layers. The nanowalls are grown to produce a variety of shapes from simple circles to text at the microscale. The selective growth of high quality ZnO nanowalls is investigated by electron microscopy and optical spectroscopy. The hybrid nanostructure can be exploited to fabricate various nanodevices including microarrays of nanotube LEDs. </P>

Co^60-r선 조사에 따른 이축연신 폴리프로필렌 필름의 열자격전류 특성 변화

류부형,김민,김규백 동국대학교 경주대학 1997 東國論集 Vol.16 No.1

In order to investigate the Co^60-γ ray irradiation effects on the thermally stimulated current(TSC) characteristics of the biaxially-oriented polypropylene (BOPP) films, TSCs on the 15㎛ thick BOPP films before and after irradiation were observed in the temterature range of -120∼120℃ and at the forming electric field of 10, 30 and 60MV/m. Two distinguished peeaks such as β peak and α peak, the former was regarded as the depolarization process of dipoles on a amorphous region and the latter was attributed the thermally excited detrapping process of the trapped ions on a crystaline region in BOPP, were observed in the TSC spectra. From the observed results, the physical properties of γ-ray irradiated BOPP that are activation energy and relaxation time of charged particles, moment of dipole and forming field dependencies on TSC characteristics were analyzed and discussed.

신축학교 실내공기질이 초등학생들의 인지기능에 미치는 영향

김태섭,김선주,박시영,전만중,김규태,김창윤,정종학,백성옥,사공준 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.1

목적: 신축초등학교와 대조학교를 선정하여 교실 내 포름알데히드와 TVOCs를 측정하고 컴퓨터 신경행동검사를 이용하여 실내공기 오염물질이 학생들의 인지기능에 미치는 영향을 평가하였다. 방법: 신축초등학교 5학년 71명을 실내공기 오염물질 노출군으로 하고, 개교한지 12년째인 대구시의 일개 초등학교 5학년 2학급 63명을 대조군으로 하여 실내공기 오염물질농도 측정 및 컴퓨터 신경행동검사를 실시하였다. 결과: 신축학교에서 의도적으로 창문을 개방한 학급에서 1교시 포름알데히드 농도는 34.6 ㎍/㎥, 4교시에는 27.3 ㎍/㎥, 창문을 개방하지 않은 학급에서 1교시 80.2 ㎍/㎥, 4교시 127.1 ㎍/㎥, 대조학교에서는 2학급 각각 1교시에 12.6 ㎍/㎥, 7.5 ㎍/㎥, 4교시에 19.8 ㎍/㎥, 25.2 ㎍/㎥였다. TVOCs의 경우 신축학교의 의도적으로 창문을 개방한 학급에서 1교시에 농도는 487.2 ㎍/㎥, 4교시에 428.2 ㎍/㎥, 창문을 개방하지 않은 학급에서 1 교시에 농도는 1,283.5 ㎍/㎥, 4교시에 1,715.4 ㎍/㎥, 대조학교의 경우 2학급에서 각각 1교시에 농도는 240.9 ㎍/㎥, 150.8 ㎍/㎥, 4교시에 농도는 371.9 ㎍/㎥, 448.0 ㎍/㎥였다. 부호숫자 짝짓기의 평균반응시간은 대조학급이 1교시에 2,585 msec에서 4교시에 2,459 msec으로 감소한 반면 신축학교의 창문을 의도적으로 개방한 학급은 2,343 msec에서 2,510 msec으로, 평소대로 수업한 학급은 2,340 msec에서 2,563 msec으로 반응시간이 각각 167 msec, 223 msec 증가하였다. 1교시의 부호숫자 짝짓기 의 반응시간을 100%로 했을 때 창문을 의도적으로 개방 한 학급은 4교시에 5.9% 증가하였고(p<0.01), 평소대로 수업한 학급은8.3% 증가하였다(p<0.01). 반면에 대조학급은 4교시에 5.0% 감소하였다(p<0.01). 결론: 향후 학교의 실내공기질의 안전성 평가는 샘플링 된 공기의 오염수준의 측정뿐만 아니라 학생들의 건강영향도 함께 고려되는 것이 바람직할 것이다. Objectives: Most studies on the indoor air quality of newly built schools have focused only on the number of schools exceeding a standard or on subjective symptoms, such as sneezing, irritated eyes and an irritated nose. However, there has been no report on how poor indoor air quality affects children's academic performance. This study evaluated the effects of indoor air quality on the neurobehavioral performance of elementary school children. Methods: This study measured the indoor air pollutants and compared children's performance, using a computerized neurobehavioral test, between a newly built and a 12-year-old school. In addition, a questionnaire was administered regarding the indoor air quality related symptoms of children in a newly built and a 12-year-old school. Results: In the newly built school, the formaldehyde levels in the 1^(st) and 4^(th) classes with an open window were 34.6 and 27.3 μg/㎥, respectively. The levels in the 1^(st) and 4^(th) classes with the window closed were 80.2 and 127.1 μg/㎥, respectively. In the 12-year-old school, the formaldehyde levels in 1^(st) and 4^(th) class with open and closed window were 12.6 and 7.5 μg/㎥, and 19.8 and 25.2 μg/㎥, respectively. With respect to total volatile organic compounds, the levels in the 1^(st) and 4^(th) classes with the window open in the newly built school were 87.2 and 428.2 μg/㎥, respectively. In the 1^(st) and 4^(th) classes with the window closed, the total volatile organic compounds were 1,283.5 and 1,715.4 μg/㎥, respectively. In the 12-year-old school, the total volatile organic compounds were 240.9 and 150.8 μg/㎥, and 371.9 and 448.0 μg/㎥ in the 1^(st) and 4^(th) classes, respectively. In the case of a newly built school, the performance of the students in the neurobehavioral test in the 4^(th) class with the window closed was 8.3% lower than for those in the 1^(st) class with the window closed, but was only 5.9% lower when the windows were open. There was a statistical significant difference in the total numbers of symptoms between the two schools. Conclusions: These results suggest a proper evaluation of the indoor air quality within schools is required into both the level of contamination and the health effects of these contaminants toward children.

Transarterial Chemoebolization-Stereotactic Body Radiation Therapy versus Transarterial Chemoebolization for ≤5cm Hepatocellular Carcinoma: Propensity Score Matching

( Baek Gyu Jun ),( Sang Gyune Kim ),( Young Don Kim ),( Gab Jin Cheon ),( Koon Hee Han ),( Jeong-ju Yoo ),( Young Seok Kim ),( Soung Won Jeong ),( Jae Young Jang ),( Sae Hwan Lee ),( Suyeon Park ),( H 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Patients with liver cirrhosis and hepatocellular carcinoma (HCC) are often ineligible for resection or local ablation therapy due to poor liver function and/or difficult location. The aim of this study is to evaluate therapeutic outcomes of stereotactic body radiotherapy (SBRT) combined with transarterial chemoembolization (TACE) compared with TACE alone for HCC measuring less than 5 cm. Methods: From March 2011 to December 2016, 85 patients underwent SBRT with TACE (SBRT-TACE group) and 114 underwent TACE (TACE group) at 4 tertiary hospitals. Local control rate (LCR), progression-free survival (PFS) and overall survival (OS) were compared after propensity-score matching (1:1 ratio). Results: The SBRT-TACE group showed significantly higher 1- and 3-year LCR than the TACE group (91.1% and 89.9%, respectively vs 69.9% and 44.8%, respectively; P<0.001). The SBRT-TACE group showed better 1- and 3-year PFS than the TACE group (56.5% and 32.3%, respectively vs 42.2% and 21.6%, respectively; P=0.022). However, 1-, 3- and 5-year OS was not different between the SBRT-TACE and TACE groups (98.8%, 89.1% and 80.7%, respectively vs 99.7%, 83.3% and 71.0%, respectively; P=0.206). In multivariate analysis, the overall SBRT added to TACE did not contribute to extend PFS. However, in patients with less than 2 tumors, the combined therapy was effective (HR 0.590, 95% CI 0.392-0.889, P=0.012). Conclusions: SBRT-TACE is superior to TACE in terms of LCR. Particularly, SBRT-TACE may be an effective alternative in patients with HCC number (≤2), which is not indicated for resection or local ablation.

Radiation Induced Liver Disease after Stereotactic Body Radiation Therapy for Small Hepatocellular Carcinoma : Risk Factor and Clinical Significance

( Baek Gyu Jun ),( Young Don Kim ),( Gab Jin Cheon ),( Sae Hwan Lee ),( Hong Soo Kim ),( Sang Gyune Kim ),( Young Seok Kim ),( Boo Sung Kim ),( Soung Won Jeong ),( Jae Young Jang ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: The aim of this study was to identify parameters that predict radiation induced liver disease (RILD) following stereotactic body radiotherapy (SBRT) in cirrhotic patients with small hepatocellular carcinoma (HCC). Methods: We retrospectively reviewed 84 patients treated with SBRT for small (diameter <3 cm) HCC treated by SBRT between March 2011 and February 2015. RILD was defined as elevated liver transaminases more than five times the upper limit of normal or a worsening of Child-Pugh score by 2 within 3 months after SBRT. All patients were assessed at 1 month and every 3 months after SBRT. Results: Median follow-up was 16.5 (2-56) months after SBRT. Seventeen (18.5%) of the 84 patients developed RILD after SBRT. Multivariate logistic regression analysis showed that Child-Pugh (CP) scores (p < 0.01) was significant parameter to predict RILD in cirrhotic patients. According to linear by linear association model, as CP score increases, the incidence rate of RILD (P<0.01) increases, and the recov- ery rate of RILD (P<0.01) shows a tendency to decrease. The incidence rate of RILD increases at CP score 6 remarkably, and the recovery rate of RILD seems to decrease significantly at CP score 8. In multivariate analysis, CP score 8 was independent prognostic factors of overall survival. (P<0.01) Conclusions: CP score is important factor to predict RILD. The Incidence rate of RILD seems to increase, but does not recover at CP score above 8.