Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

Lee, Eun-Mi,Lee, Young-Eun,Lee, Esder,Ryu, Gyeong Ryul,Ko, Seung-Hyun,Moon, Sung-Dae,Song, Ki-Ho,Ahn, Yu-Bae Korean Diabetes Association 2011 Diabetes and Metabolism Journal Vol.35 No.5

<P><B>Background</B></P><P>Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads to islet dysfunction and induces apoptosis in pancreatic β-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic and cytoprotective gene. The purpose of this study is to investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect pancreatic β-cells from high glucose-induced apoptosis.</P><P><B>Methods</B></P><P>Reverse transcription-polymerase chain reaction was performed to analyze the CoPP-induced mRNA expression of HO-1. Cell viability of INS-1 cells cultured in the presence of CoPP was examined by acridine orange/propidium iodide staining. The generation of intracellular reactive oxygen species (ROS) was measured using flow cytometry. Glucose stimulated insulin secretion (GSIS) was determined following incubation with CoPP in different glucose concentrations.</P><P><B>Results</B></P><P>CoPP increased HO-1 mRNA expression in both a dose- and time-dependent manner. Overexpression of HO-1 inhibited caspase-3, and the number of dead cells in the presence of CoPP was significantly decreased when exposed to high glucose conditions (HG). CoPP also decreased the generation of intracellular ROS by 50% during 72 hours of culture with HG. However, decreased GSIS was not recovered even in the presence of CoPP.</P><P><B>Conclusion</B></P><P>Our data suggest that CoPP-induced HO-1 up-regulation results in protection from high glucose-induced apoptosis in INS-1 cells; however, glucose stimulated insulin secretion is not restored.</P>

Evaluation for Impacts of Nitrogen Source to Groundwater Quality in Livestock Farming Area

Gyeong-Mi Lee,Sunhwa Park,Ki-In Kim,Sang-Ho Jeon,Dahee Song,Deok-hyun Kim,Tae-Seung Kim,Seong-Taek Yun,Hyen Mi Chung,Hyun-Koo Kim 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.5

We investigated 52 livestock farming complexes in Gyeong-Gi and Incheon provinces based on low, medium, and high livestock density and groundwater quality. The objective of this study was to evaluate a relationship between nitrate N concentration in groundwater and animal factors, such as livestock density and animal species. 2,200 groundwater samples for 3 years from 2012 to 2014 at Gyeong-Gi and Incheon provinces were collected and analyzed for pH, EC, DO, ORP, temperature, major anions and cations, such as NO₃-N, HCO₃<SUP>-</SUP>, PO₄<SUP>-</SUP>, SO₄<SUP>2-</SUP>, Cl<SUP>-</SUP>, NH₄-N, K<SUP>+</SUP>, Na<SUP>+</SUP>, Ca<SUP>2+</SUP>, Mg<SUP>2+</SUP>, T-N, and TOC. Average concentration of total N for generated load density was 23,973 g day<SUP>-1</SUP> km<SUP>-2</SUP> for cattle, 51,551 g day<SUP>-1</SUP> km<SUP>-2</SUP> for pig, and 52,100 g day<SUP>-1</SUP> km<SUP>-2</SUP> for poultry. For animal feeding species, average ratio for generated load over discharge load was 16.1% for cattle, 7.8% for pig, and 7.1% for poultry. Therefore, cattle feeding region is highly vulnerable for water pollution compared to pig and poultry feeding areas. The concentrations of chloride, nitrate, and total N in the groundwater samples were higher at high animal farming regions than other regions. The average concentration of nitrate, and chloride in groundwater samples was 5.0 mg L<SUP>-1</SUP>, 16.6 mg L<SUP>-1</SUP> for low livestock density, 6.9 mg L<SUP>-1</SUP>, 17.7 mg L<SUP>-1</SUP> for medium livestock density and 7.6 mg L<SUP>-1</SUP>, 22.7 mg L<SUP>-1</SUP> for high livestock density and total nitrogen (T-N) was 7.7 mg L<SUP>-1</SUP> for low livestock density, 9.4 mg L<SUP>-1</SUP> for medium livestock density, 10.7 mg L<SUP>-1</SUP> for high livestock density. In conclusion, based on this research, for managing groundwater quality near livestock farming regions, Ca-(Cl+NO₃) group from the Piper diagram is more efficient than using 19 factors for water quality standard.

Evaluation for Impacts of Nitrogen Source to Groundwater Quality in Livestock Farming Area

Lee, Gyeong-Mi,Park, Sunhwa,Kim, Ki-In,Jeon, Sang-Ho,Song, Dahee,Kim, Deok-hyun,Kim, Tae-Seung,Yun, Seong-Taek,Chung, Hyen Mi,Kim, Hyun-Koo 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.5

We investigated 52 livestock farming complexes in Gyeong-Gi and Incheon provinces based on low, medium, and high livestock density and groundwater quality. The objective of this study was to evaluate a relationship between nitrate N concentration in groundwater and animal factors, such as livestock density and animal species. 2,200 groundwater samples for 3 years from 2012 to 2014 at Gyeong-Gi and Incheon provinces were collected and analyzed for pH, EC, DO, ORP, temperature, major anions and cations, such as $NO_3-N$, ${HCO_3}^-$, ${PO_4}^-$, ${SO_4}^{2-}$, $Cl^-$, $NH_4-N$, $K^+$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$, T-N, and TOC. Average concentration of total N for generated load density was $23,973g\;day^{-1}\;km^{-2}$ for cattle, $51,551g\;day^{-1}\;km^{-2}$ for pig, and $52,100g\;day^{-1}\;km^{-2}$ for poultry. For animal feeding species, average ratio for generated load over discharge load was 16.1% for cattle, 7.8% for pig, and 7.1% for poultry. Therefore, cattle feeding region is highly vulnerable for water pollution compared to pig and poultry feeding areas. The concentrations of chloride, nitrate, and total N in the groundwater samples were higher at high animal farming regions than other regions. The average concentration of nitrate, and chloride in groundwater samples was $5.0mg\;L^{-1}$, $16.6mg\;L^{-1}$ for low livestock density, $6.9mg\;L^{-1}$, $17.7mg\;L^{-1}$ for medium livestock density and $7.6mg\;L^{-1}$, $22.7mg\;L^{-1}$ for high livestock density and total nitrogen (T-N) was $7.7mg\;L^{-1}$ for low livestock density, $9.4mg\;L^{-1}$ for medium livestock density, $10.7mg\;L^{-1}$ for high livestock density. In conclusion, based on this research, for managing groundwater quality near livestock farming regions, $Ca-(Cl+NO_3)$ group from the Piper diagram is more efficient than using 19 factors for water quality standard.

Antioxidant and hepatoprotective effects of Korean ginseng extract GS-KG9 in a D-galactosamine-induced liver damage animal model

Yun Ho Jo,Hwan Lee,Myeong Hwan Oh,Gyeong Hee Lee,You Jin Lee,Ji Sun Lee,Min Jung Kim,Won Yong Kim,Jin Seong Kim,Dae Seok Yoo,Sang Won Cho,Seon Woo Cha,Mi Kyung Pyo 한국영양학회 2020 Nutrition Research and Practice Vol.14 No.4

BACKGROUND/OBJECTIVES: This study was designed to investigate the improvement effect of white ginseng extract (GS-KG9) on D-galactosamine (Ga1N)-induced oxidative stress and liver injury. SUBJECTS/METHODS: Sixty Sprague-Dawley rats were divided into 6 groups. Rats were orally administrated with GS-KG9 (300, 500, or 700 mg/kg) or silymarin (25 mg/kg) for 2 weeks. The rats of the GS-KG9- and silymarin-treated groups and a control group were then intraperitoneally injected Ga1N at a concentration of 650 mg/kg for 4 days. To investigate the protective effect of GS-KG9 against GalN-induced liver injury, blood liver function indicators, anti-oxidative stress indicators, and histopathological features were analyzed. RESULTS: Serum biochemical analysis indicated that GS-KG9 ameliorated the elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in GalN-treated rats. The hepatoprotective effects of GS-KG9 involved enhancing components of the hepatic antioxidant defense system, including glutathione, glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). In addition, GS-KG9 treatment inhibited reactive oxygen species (ROS) production induced by GalN treatment in hepatocytes and significantly increased the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins, which are antioxidant proteins. In particular, by histological analyses bases on hematoxylin and eosin, Masson"s trichrome, α-smooth muscle actin, and transforming growth factor-β1 staining, we determined that the administration of 500 mg/kg GS-KG9 inhibited hepatic inflammation and fibrosis due to the excessive accumulation of collagen. CONCLUSIONS: These findings demonstrate that GS-KG9 improves GalN-induced liver inflammation, necrosis, and fibrosis by attenuating oxidative stress. Therefore, GS-KG9 may be considered a useful candidate in the development of a natural preventive agent against liver injury.

경상북도 후포와 강원도 장호에서 정치망으로 채집된 어류 종조성 비교

강정하 ( Jung Ha Kang ),김이경 ( Yi Gyeong Kim ),박중연 ( Jung Youn Park ),김진구 ( Jin Koo Kim ),유정화 ( Jung Hwa Ryu ),강충배 ( Chung Bae Kang ),박정호 ( Jeong Ho Park ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.4

Two major temperature fronts, the Subpolar (Gosung, Gang-won-do; 38°-41° N) and Thermal (Jukbyun, Gyeong-sang-buk-do; 36°-37° N) fronts, are found in the East Sea along the east coast of Korea. These are located roughly where the Tsushima Warm Current and North Korea Cold Current intersect. To clarify the effect of the Thermal Front, we investigated seasonal variation in fish species composition using set nets in two areas located north (Jangho, Gang-won-do) and south (Hupo, Gyeong-sang-buk-do) of Jukbyun, Gyeong-sang-buk-do, and compared the sea water temperature and salinity. We collected a total of 38 fish species in Hupo and 25 in Jangho. Trachurus japonicus was the most common species at both sites, but the subdominant species differed. At Hupo, the subdominant species were Konosirus punctatus and Diodon holocanthus, whereas Clupea pallasii and Scomber japonicus were subdominant at Jangho. Based on Froese and Pauly (2014), subtropical fishes accounted for 55% of fish in Hupo but only for 33% in Jangho. The difference in fish species composition was most obvious in May and August. According to the Korea Hydrographic and Oceanographic Administration, sea surface temperature and salinity were slightly higher at Hupo than at Jangho. Our findings suggest that the oceanographic boundary resulting from the Thermal Front near Jukbyun, Gyeong-sang-buk- do may have a major effect on the distribution of migratory fish species.

마이크로니들의 형태 및 적용방법이 약물의 피부 투과에 미치는 영향

임지호,이경록,이은주,조정원 충남대학교 약학대학 의약품개발연구소 2012 藥學論文集 Vol.27 No.-

The purpose of this study is to develop the microneedle using biodegradable and biocompatible polymer and to pioneer the new route of drug delivery through the research to apply the most desirable administration form of small molecules, peptides, proteins, genes, biological products including vaccines. Various biocompatible microneedles were manufactured. To apply drug to microneedle, drug formulation using ethylene vinyl acetate patch or polyacryl acid gel was developed. Drug permeation into rat skin based on the application method of patch or gel with microneedle was studied using Franz diffusion cell system. In vitro drug permeation results showed that the permeation by the application of hollow microneedle was not increased compared with solid microneedle. When G60 or G60*2 microneedle was used, the permeated amount of calcein was increased compared to when solid microneedle was used. The permeated amount of lidocaine HCl in 12 hr was increased when microneedle was used was increased. Skin irritation followed by microneedle showed there was little difference in the decrease of redness after microneedle application based on application time.

음의 세기를 이용한 음향파워 측정

장호경 慶山大學校 基礎科學硏究所 2002 基礎科學 Vol.6 No.2

음향파워는 일반적으로 음압과 음의 세기 측정으로부터 결정된다. 본 연구에서는 음향파워의 측정을 위해 음원으로부터의 거리, 주사형태와 속력 등 측정변수의 영향과 음의 세기 측정의 실제적인 방법을 연구하였다. 음원의 음향파워레벨을 얻기 위하여 포인트법(point method)과 스캔법(scan method)으로 음원의 표면에서 음의 세기를 측정하였다. 두 개의 가깝게 설치된 마이크로폰을 이용하여 측정하는 음의 세기에 대한 근사오차를 해석하였다. 음원으로부터 측정거리에 따른 효과를 해석한 결과 두 방법에 대하여 최대의 차이는 1.5㏈와 1.7㏈를 얻었다. 그리고 두방법 모두 음원으로부터 먼거리에서 더 정확한 결과들이 얻어졌다. The sound power is normally determined from sound pressure or sound intensity measurements. Practical investigation of the sound intensity measurements and the influence of some measurement parameters, such as the distance from the source, scanning pattern and speed, on sound power determination has been presented in this study. Sound intensity is measured at the surfaces by the scanning and point methods to obtain the sound power level of the source. The approximation errors of measuring sound intensity using two closely spaced microphones are examined. A maximum difference of 1.5㏈ and 1.7㏈ is obtained for the point and sweep methods, respectively. Fairly accurate results are obtained at larger distances.

음의 세기 측정에서 근사오차 해석

장호경 慶山大學校 基礎科學硏究所 1998 基礎科學 Vol.2 No.2

두 개의 가깝게 설치된 마이크로폰을 이용하여 측정하는 음의 세기에 대한 근사오차를 연구하였다. 두 개의 마이크로폰과 스피커 중심점 사이의 거리를 1.5m로 설치하고, 무음향실에서 마이크로폰 탐침자의 방향 응답특성을 조사하였다. face to face와 side by side en 배열에 대한 주파수와 음압 응답특성을 측정하였으며, 측정된 음압으로부터 환산한 음의 세기와 두 개의 마이크로폰으로부터 직접 측정한 음의 세기값 사이의 근사오차를 해석하였다. 125Hz∼6.3kHz의 주파수 영역에서 분리거리 12mm일 때 음의 세기의 근사오차가 ±1dB 이하임을 확인하였다. The approximation errors of measuring sound intensity using two closely spaced microphones are examined in this paper. To determine the directional response of an microphone probe, the microphones are mounted on a turntable in an anechoic chamber, about 1·5m distant from a loudspeaker. The frequesncies and pressure responses obtained experimentally, are presented for both face to face(FF) and side by side(SS) microphone configurations. The measuring approximation errors are estimated by checking the difference between the measured sound pressure and sound intensity. For the microphone separation of l2mm, the approximation errors are shown to he less than ±1dB from 125Hz to 6.3kHz.

유한한 임피던스 지표면 위의 소음전파에 관한 연구

장호경 慶山大學校 基礎科學硏究所 2000 基礎科學 Vol.4 No.1

In this study, propagation of noise over ground of finite impendance is investigated to construct a simple noise propagation medel. This model makers it possible to predict the sound level, L??, of noise from a stationary source and the time-average sound level, L??? of noise from moving sources. This model is then used for the road traffic noise situation, and the theoretical excess attenuation is compared to values obtained from road traffic noise measurements. The measured and the calculated results agree well for excess attenuation between two points near a road.

산업용 이차전지의 열화진단에 관한 연구

장대경,황호석,박민기 서울産業大學校 2005 논문집 Vol.54 No.3

This paper proposes a new degradation diagnosis method of secondary battery in order to improve a battery economy. In the proposed method, a bad cell is detected by the measurement of DC internal resistance and the battery capacity is estimated by the electric discharge test in a short period of time, which can be used to determine the time to replace the battery. Through the computer simulation and actual experimentation, the proposed method's validity is confirmed by comparing and analyzing the performance. As a result, we show that it is possible to estimate the accurate capacity of secondary battery in a short space of time.