부산근해 유류분해세균의 생태학적 특성

남귀숙,이건,이상준 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-

만성적인 유류 오염지역으로 간주되는 부산근해 10곳의 해수를 채취, 이화학적 특성과 이곳 해양 생태계내에 존재하는 종속영양세균수 및 유류분해 세균수, 그리고 그 분포비를 조사하였다. 해수내 유분의 농도와 일반 종속영양세균수에 대한 유류분해세균수의 생태계 내 분포비가 수영만 일대와 부산항 일대에서 높은 수치로 나타나 부산항 일대와 수영만 일대의 유류오염 정도가 타지역보다 높음을 알 수 있었으며, 오염된 유류가 해양생태계내에서 stress 인자로 작용, 유류분해 미생물의 군집내 증가현상을 나타내었다. 수차례의 농화배양을 거쳐 얻은 유류분해 세균 227개 균주중 분해능이 비교적 우수한 112개 균주만을 선정 동정한 결과, 환경정화자로서 주로 알려져 있는 종속영양세균인 Gram음성 간균의 Pseudomonas속, Enterobactericeae 관련속, Acinetobacter속 등의 11개 속과 Gram 양성 구균인 Micrococcus속 들로 나타났으며, 장내세균의 출현은 해양내 오수 유입을 반영하고 있었다. 배양적 특성, 생화확적 특성 및 생리적 특성 등 54개의 인자들을 이용하여 60% 유사도 수준으로 수리분류를 실시한 수 유류분해에 관련되는 미생물속 간의 유연관계를 조사한 결과 Pseudomonas속인 38개 세균들로 구성되어진 cluster 5 등 3개의 sp. 및 Pseudomonas sp.들로 구성된 cluster 2 그리고 Moraxella sp.로 구성된 cluster 5 등 3개의 Major cluster와 12개의 Minor cluster로 분석되었다. 종다양성 분석 결과, 정상적인 해양생태계 보다 부산항 일대 해양생태계에서의 종다양성 수치가 낮게 나타남으로써 유류유입이 생태계 안정성을 파괴함을 알 수 있었다. 또한, 부산근해 해양생태계내의 유류분해에 작용하는 세균중 Pseudomonas속이 우세한 생태학적 지위를 나타내었다. The bacteria capable of degrading and utilizing petroleum compounds as sole carbon and energy source were isolated from the seawater at 10 sites in pusan costal area as regarded the petroleum pollution zone, tested the physicochemical properties, and enumerated active heterotrophes and petroleum degraders. The results, examined for the distribution ratio of heterotrophes and petroleum degraders and the concentrations of petroleum in sea water, were high in Suyoung bay and Pusan harbor, do we could conclude that the area of there might be more polluted than others, and the petroleum polluted in marine ecosystem should act as stress factor which affected population. Taxonomical positions of 112 isolates were investigated. The results of identification were showed 11 genera of Gram nagative bacillus or cocobacillus which were Pseudomonas spp., Enterobacteriaceae, and Acinetobacter spp., etc., and 1 genus, Micrococcus spp., were Gram positive coccus. Apparence of Enterobacteriaceae indicated that the sea water was polluted with waste water. As a results of numerical taxonomy based on Jacchard coefficient, three major clusters and twelve minor clusters had been showed at the level of 60% similarity. Species diverisity analysis about petroleum degraders in the researched Pusan marine ecosystem had lower value than that in healthy one. Also genus of Pseudomonas had predominant ecological niche in petroleum degrading bacteria on Pusan costal area.

자동차용 충격 흡수기의 동특성 연구

남경탁,황성원,신귀수 한국산업안전학회 2002 한국안전학회지 Vol.17 No.3

A dynamic characteristics of shock absorber in the various excitation is investigated experimentally. Work diagrams and characteristic curves are used as a experimental standard. The various excitation conditions, temperature and noise are very important factors in associated with the reduction of damping force. It is found that the heat occurrence from shock absorber, the gas shock absorber is much than oil shock absorber and increased in high speed. As to the variation of damping force, there are no change when the speed is low, but we fixed amount of variation by increasing speed and change of new and old decrease. The sound pressure of the swash noise from cycle of shock absorber, we compared with theory sound pressure by experiment.

저온소결 세라믹을 이용한 적층형 전압제어발진기의 설계제작

朴貴南,宋眞亨,金志均,李憲用 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

The circuit substrate was made from the LTCC that a ε_(r) was 7.8. Accumulated Varactor and the low noise transistor which were a Surface Mount Device-type element on LTCC substrate. Let passive element composed R, L, C with strip-line of three dimensions in the multilayer substrate circuit inside, and one structure integrated band-pass filter, resonator, a bias line and a matching circuit. Used Screen-Print process, and made Strip-line resonator. A design produced and multilayer-type VCO and recognized a characteristic with the Spectrum Analyzer which was measurement equipment. Measured multilayer structure VCO is oscillation frequency 1292[MHz], oscillation output -28.38[dBm], harmonics characteristic -45[dBc] in control voltage 1.5[V]. A phase noise is -68.22[dBc/Hz] in 100 [kHz] offset frequency. The oscillation frequency variable characteristic showed 30[MHz/V] characteristic, and consumption electric current is approximately 10[mA].

실내 스모그 챔버의 설계 및 성능평가

배귀남,김민철,이승복,송기범,진현철,문길주 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.4

A multi-functional indoor smog chamber was designed and evaluated lo investigate photochenical or water vapor reaction mechanisms of air pollutants. Various smog chamber experiments could be conducted using ambient air or purified air in this smog chamber. The smog chamber consisted of a housing, a Teflon bag, blacklights, injection ports, sampling ports, and utility facilities. The characteristics of light source, the wall losses of air pollutants, and the quality of purified air were experimentally investigated. The maximum NO₂ photolysis rate was 1.10min^(-1). In a 2.5-m³ Teflon bag, the wall losses of ambient O₃, NO, and NO₂were 1.2~2.4×10^(-3)min^(-1), 0.7~2.0×10^(-3)min^(-1), and 0.4-2.0×10^(-3)min^(-1), respectively. The wall loss of ambient particles ranging 0.05 to 0.2㎛ was 1.8~5.4×10^(-3)min^(-1), which was slightly higher than those of ambient gaseous species. The purified air supply system provided high quality of air with NO₂, < 1 ppb, and total hydrocarbons <5 ppb.

튜브 전기로를 이용한 TiO₂ 나노입자의 합성 및 특성 분석

배귀남,현정은,이태규,정종수 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.3

Titania particles are widely used as a photocatalyst to treat various contaminants in air and water. Titania particles were formed by vapor-phase oxidation of titanium tetraisopropoxide (TTIP) in a tube furnace between 773 and 1,273 K. The effect of process variables such as furnace temperature. flow rate of carrier air. and flow rate of sheath air on powder size and phase characteristics was investigated using a scanning mobility particle sizer (SMPS), transmission electron microscopy (TEM) and X-ray diffraction (XRD). The size distribution of synthesized titania particles was characterized with mode diameter and peak concentration. The mode diameter ranging from 20 to 80 nm decreased with increasing flow rates of sheath air and carrier air, and increased with increasing furnace temperature. The peak concentration increased with increasing flow rates of sheath air and carrier air. The best synthetic condition for high production rate can be derived from the experimental data set represented by mode diameter and peak concentration. The crystal structure of synthesized titania particles was found to be anatase phase, ensuring high photocatalytic potential.

황해 직선 황로상 대기오염물질의 측정

이승복,배귀남,진현철,김영성,문길주,심상규 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.1

Air pollutants were measured eight times from June 1999 to June 2002 on regular ferries cruising across the Yellow Sea between Incheon in Korea and Qingdao or Tianjin in China. PM_(10) and PM_(2.5_ were measured as particulate matters and SO₂, CO, and NOx were measured as gaseous pollutants. On each route, sampling was made, starting two hour after departure and ending two hour before arrival. Low concentrations of gaseous pollutants that were not much varied according to sampling period and location revealed that atmosphere over the sea was not directly affected by anthropogenic emissions. However, concentrations of fine particles were generally higher than those measured at Deokjeok Island. 50 km west of the western seashore, at similar periods. It was believed that considerable influence of China in the form of secondary pollutants was exerted over the sea.

초기 톨루엔 농도가 톨루엔-NOx-공기 혼합물의 광산화 반응에 미치는 영향 : I. 가스상 물질의 변화 I. Change of Gaseous Species

이영미,배귀남,이승복,김민철,문길주 한국대기환경학회 2005 한국대기환경학회지 Vol.21 No.1

An experimental investigation of the gas-phase photooxidation of toluene-NO_(x)-air mixtures at sub-ppm concentrations has been carried out in a 6.9 ㎥, indoor smog chamber irradiated by blacklights. Measured parameters in the toluene-NOx experiments included O₃, NO, NO₂, NO_(x), CO, SO₂, toluene, and air temperature. The initial toluene concentration ranged from 225 ppb to 991 ppb and the initial concentration ratio of toluene/NOx in ppbC/ppb was in the range of 5~20. It was found that the variation of gaseous species with irradiation time caused by the photooxidation of toluene-NOx-air mixtures depended on the initial toluene concentration for similar concentration ratio of toluene/NOx. The dependency of initial toluene concentration on the photooxidation of toluene-NO_(x)-air mixtures for toluene/NOx =5~6 seemed to be opposite to that for toluene/NOx=10~11. The arriving time at maximum ozone concentration depended on both initial toluene concentration and initial concentration ratio of toluene/NOx. However, the maximum concentration of ozone formed by photooxidation depended only on the initial toluene concentration.

스모그 챔버에서 수분 반응에 의한 대기 에어로졸의 생성 및 성장

김민철,배귀남,문길주,박주연 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.2

Aerosol formation and growth by water vapor reactions were investigated in a 2.5-㎥ indoor smog chamber filled with the unfiltered ambient air. The relative humidity of test ambient air was elevated at 59~64% or 84~88% by adding water vapor. The aerosol number size distribution and the concentrations of O₃, NO, NO₂, and SO₂ were measured during the experiments. The O₃, and NO₂ gases were well reacted with the water vapor at high relative humidity of 84~88%, and the reaction rates of these gases seemed to be decreased at low relative humidity of 59~64%. The formation and condensational growth phenomena of ambient aerosols by water vapor reactions were observed in a Teflon bag, depending strongly on the initial particle size distribution. The water vapor reactions might be affected by the contents of oxidants produced by photochemical reactions under sunlight.

초기 톨루엔 농도가 톨루엔-NO_(x)-공기 혼합물의 광산화 반응에 미치는 영향 : II. 입자상 물질의 생성 및 성장 II. Aerosol Formation and Growth

이영미,배귀남,이승복,김민철,문길주 한국대기환경학회 2005 한국대기환경학회지 Vol.21 No.1

An experimental investigation of the gas-phase photooxidation of toluene-NOx-air mixtures at sub-ppm concentrations has been carried out in a 6.9 ㎡, indoor smog chamber irradiated by blacklights. Measured parameters in the toluene-NO_(x) experiments included aerosol, O₃, NO, NO₂, NO_(x), CO, SO₂, toluene, and air temperature. The initial toluene concentration ranged from 225 ppb to 991 ppb and the initial concentration ratio of toluene/NOx in ppbC/ppb was in the range of 5~20. It was found that the variation of aerosol number concentration with irradiation time caused by the photooxidation of toluene-NOx-air mixtures depended on the initial toluene concentration for similar concentration ratio of toluene/NO_(x). The dependency of initial toluene concentration on the photooxidation of toluene-NO_(x)-air mixtures for toluene/NOx=5~6 seemed to be opposite to that for toluene/NO_(x)= 10~11. The maximum number concentration of aerosols formed by photooxidation and the aerosol yield depended on both initial toluene concentration and initial concentration ratio of toluene/NO_(x). In this study, the aerosol yield, defined as aerosol formed per unit toluene consumed, was found to be 0.01~0.16.

위장관 출혈을 일으킨 공장의 형활근종 1예

김병수,장성종,부귀범,조운택,박찬국,김만우,변주남,김정용,전호종 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Small bowel leiomyoma is a rare tumor which may occur wherever smooth muscle is present. The small bowel is the most common site and it makes up 20~30% of all benign small intestinal neoplasms. A spectrum of symptoms are common ranging from nausea, vomiting, abdominal pain, to massive intestinal bleeding. Bleeding is the most common symptom and is usually gross and recurrent. Occasionally it may be life threatening. But, the most serious problem is both the absence of specific clinical symptoms, and the difficulties shown in radiological diagnostics of the small bowel. If conventional diagnostic methods fail to reveal any positive findings for gastrointestinal bleeding, the diagnosis of intestinal leiomyoma should be suspected and an SMA angiography shoud be performed. The angiographic criteria of small bowel leiomyoma is very characteristic. We present a case of leiomyoma with considerable and prolonged gastrointestinal bleeding, which was diagnosed by angiography. A 61-year old woman was admitted with a history of melena over the last 10 days. An SMA angiography showed a hypervascular mass with a diameter of 3×3cm. A segmental resection was performed under the impression of the gastrointestinal bleeding due to leiomyoma of the small bowel. Microscopic examination of the lesion shows interlacing or whorl bundles of spindle shaped ces and no mitosis.