Mid-term clinical and radiographic outcomes of porous-coated metaphyseal sleeves used in revision total knee arthroplasty

( Ron Gurel ),( Samuel Morgan ),( Etay Elbaz ),( Itay Ashlenazi ),( Nimrod Snir ),( Assaf Kadar ),( Aviram Gold ),( Yaniv Warschawski ) 대한슬관절학회 2021 대한슬관절학회지 Vol.33 No.-

Background: The management of bone defects remains one of the major challenges surgeons are faced with in revision total knee arthroplasty (RTKA). Large and uncontained bone defects are traditionally managed with metaphyseal sleeves that facilitate osseointegration and have reported construct stability. While many studies have presented excellent short-term outcomes using metaphyseal sleeves, less is known on their performance in the longer term. The purpose of this study was to present our mid-term results of the metaphyseal sleeves used in patients undergoing RTKA. Materials and methods: Between January 2007 and January 2015, 30 patients underwent RTKA with the use of a CCKMB prosthesis combined with an osteointegrative sleeve. The main indications for RTKA were instability in 40% of the cases (n = 12), aseptic loosening in 30% (n = 9), infection in 26.7% (n = 8), and “other” in 3.3% (n = 1). The minimal follow-up time was 5 years and the mean follow-up time was 82.4 months (SD = 22.6). Clinical outcomes were assessed by Knee Society scores (KSS), range of motion and rate of re-operation. Results: The mean Knee Society score increased significantly from 72.1 preoperatively to 90.0 postoperatively (p < 0.001). The cumulative incidence of re-operation in our study was 13.3% (n = 4). Our study reported no cases of aseptic loosening or mobile-bearing spin-out. Knee flexion to 90° and more was impossible in seven cases (23.3%) preoperatively and in one case (3.3%) postoperatively. Conclusion: Porous-coated metaphyseal sleeves demonstrated excellent rates of survivorship and radiographic ingrowth in the mid-term setting. However, further studies are required to assess their outcomes in the long-term.

Maxillary Ameloblastoma Case of a Dog

Aydın Gurel,Gulbin .ennazli,Kur.at Ozer,Ahmet Gulcubuk 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.1

In this report, an ameloblastoma case, which hasbeen occurred on the left maxilla of a 10 year-old,male,German shepherd,was described on the clinicaland morphological features. The mass with thedimensions of 5×2.5 cm was removed totally bymaxillectomy and examined by radiography, magneticresonance (MR) and biopsy. The tumor was histopa-thologically classified ameloblastoma of follicular typeand in some fields showed acanthotic form. Theinvasiveness of the tumor was also observed to behigh. This report is the first case of maxillaryameloblastoma in dogs in Turkey.

Proteins and Biomagnetics

Ogan Gurel 한국자기학회 2018 한국자기학회 학술연구발표회 논문개요집 Vol.2018 No.3

Frontal Chondrosarcoma in a Cat

F.Yildiz,A.Gurel,T.Yesildere,K.Ozer 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Chondrosarcoma in a CatF. Yildiz*, A. Gurel, T. Yesildere and K. Ozer1

Site-directed Mutagenesis of Five Conserved Residues of Subunit I of the Cytochrome cbb₃ Oxidase in Rhodobacter capsulatus

Ozturk, Mehmet,Gurel, Ekrem,Watmough, Nicholas J.,Mandaci, Sevnur Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5

Cytochrome $cbb_3$ oxidase is a member of the heme-copper oxidase superfamily that catalyses the reduction of molecular oxygen to the water and conserves the liberated energy in the form of a proton gradient. Comparison of the amino acid sequences of subunit I from different classes of heme-copper oxidases showed that transmembrane helix VIII and the loop between transmembrane helices IX and X contain five highly conserved polar residues; Ser333, Ser340, Thr350, Asn390 and Thr394. To determine the relationship between these conserved amino acids and the activity and assembly of the $cbb_3$ oxidase in Rhodobacter capsulatus, each of these five conserved amino acids was substituted for alanine by site-directed mutagenesis. The effects of these mutations on catalytic activity were determined using a NADI plate assay and by measurements of the rate of oxygen consumption. The consequence of these mutations for the structural integrity of the $cbb_3$ oxidase was determined by SDS-PAGE analysis of chromatophore membranes followed by TMBZ staining. The results indicate that the Asn390Ala mutation led to a complete loss of enzyme activity and that the Ser333Ala mutation decreased the activity significantly. The remaining mutants cause a partial loss of catalytic activity. All of the mutant enzymes, except Asn390Ala, were apparently correctly assembled and stable in the membrane of the R. capsulatus.

Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease

Burak Kuscu,Aydin Gurel 대한수의학회 2008 JOURNAL OF VETERINARY SCIENCE Vol.9 No.1

One-day-old SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus (CIAV), and the clinical development of disease and its macroscopic and microscopic alterations in the thymus and bone marrow, were observed. Tissue sections of thymus and bone marrow were stained using the streptavidin-biotin peroxidase method and examined under light microscope for evaluation of antigenic intensities in tissues. Those findings were then compared with blood parameters and ELISA results obtained through collected sera during sacrifice procedures. We sought to determine: the localization of viral antigens in thymus and bone marrow tissues after inoculation, the correlation between antigen intensities and hematologic, serologic and histopathologic findings, definitive diagnostic criteria using histopathologic and immunoperoxidase methods, and the reliability of these methods in the diagnosis of CIAV infection. For this purpose, 83, one-day-old SPF chicks were used. The birds were divided into experimental (n = 52) and control (n = 26) groups. A virus dose of TCID50 of 100,000/ml was administered intramuscularly to every bird in the experimental group. Based on the results of this study, we have suggested that clinical examination, along with macroscopic and microscopic evaluation of the thymus and bone marrow, maybe undertaken starting from day 7 post-inoculation (PI). ELISA, might be of value, as it might give consistent results starting from day 14 PI. However, the most reliable results were obtained through examination of thymus and bone marrow sections from infected birds stained by immunoperoxidase technique, as early as day 4 PI. One-day-old SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus (CIAV), and the clinical development of disease and its macroscopic and microscopic alterations in the thymus and bone marrow, were observed. Tissue sections of thymus and bone marrow were stained using the streptavidin-biotin peroxidase method and examined under light microscope for evaluation of antigenic intensities in tissues. Those findings were then compared with blood parameters and ELISA results obtained through collected sera during sacrifice procedures. We sought to determine: the localization of viral antigens in thymus and bone marrow tissues after inoculation, the correlation between antigen intensities and hematologic, serologic and histopathologic findings, definitive diagnostic criteria using histopathologic and immunoperoxidase methods, and the reliability of these methods in the diagnosis of CIAV infection. For this purpose, 83, one-day-old SPF chicks were used. The birds were divided into experimental (n = 52) and control (n = 26) groups. A virus dose of TCID50 of 100,000/ml was administered intramuscularly to every bird in the experimental group. Based on the results of this study, we have suggested that clinical examination, along with macroscopic and microscopic evaluation of the thymus and bone marrow, maybe undertaken starting from day 7 post-inoculation (PI). ELISA, might be of value, as it might give consistent results starting from day 14 PI. However, the most reliable results were obtained through examination of thymus and bone marrow sections from infected birds stained by immunoperoxidase technique, as early as day 4 PI.

Modal analysis of cracked cantilever composite beams

Kisa, Murat,Arif Gurel, M. Techno-Press 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.20 No.2

Modal analysis of cracked cantilever composite beams, made of graphite-fibre reinforced polyamide, is studied. By using the finite element and component mode synthesis methods, a numeric model applicable to investigate the vibration of cracked composite beams is developed. In this new approach, from the crack section, the composite beam separated into two parts coupled by a flexibility matrix taking into account the interaction forces. These forces are derived from the fracture mechanics theory as the inverse of the compliance matrix calculated with the proper stress intensity factors and strain energy release rate expressions. Numerical results are obtained for modal analysis of composite beams with a transverse non-propagating open crack, addressing the effects of the location and depth of the crack, and the volume fraction and orientation of the fibre on the natural frequencies and mode shapes. By means of modal data, the position and dimension of the defect can be found. The results of the study confirmed that presented method is suitable for the vibration analysis of cracked cantilever composite beams. Present technique can be easily extended to composite plates and shells.

Modal analysis of cracked cantilever composite beams

Murat Kisa,M. Arif Gurel 국제구조공학회 2005 Structural Engineering and Mechanics, An Int'l Jou Vol.20 No.2

Site-directed Mutagenesis of Five Conserved Residues of Subunit 1 of the Cytochrome cbb3 Oxidase in Rhodobacter capsulatus

( Mehmet Ozturk ),( Ekrem Gurel ),( Nicholas J. Watmough ),( Sevnur Mandaci ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.5

Enhanced somatic embryogenesis, plant regeneration and total phenolic content estimation in Lycium barbarum L.: a highly nutritive and medicinal plant

Verma Sandeep Kumar,Gantait Saikat,Mukherjee Epsita,Gurel Ekrem 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.5

Lycium barbarum (Solanaceae), commonly known as Goji (or wolfberry), is popular for its nutritive and medicinal properties and is called a “super fruit” or “super food”. Considering the importance of the economic utility, the potential of different explants (hypocotyl, leaf and root) of L. barbarumfor plant regeneration and somatic embryogenesis has been evaluated in the present study. Two sets of experiments were carried out; the first compared Murashige and Skoog (MS) medium suplemented with different concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D), N6- benzylaminopurine (BAP), thidiazuron (TDZ), kinetin, and zeatin alone, while the second set tested the combinations of TDZ with 2,4-D to inducecallus and subsequent shoot or embryo formation, respectively. For callus and subsequent shoot induction (for the first set of experiment), 2,4-D was the most effective for callus induction (100%), while TDZ at 1 mg/L produced a mean of 5 shoots per callus. For the second set of experiment spontaneous induction of somatic embryos as well as subsequent maximum shoot regeneration was recorded as 0.25 mg/L 2,4-D and 1 mg/L TDZ supplemented medium. Hypocotyl explant proved to be the most responsive organ that induced as many as 6 somatic embryos and subsequent 9regenerated shoots per callus. Later, the plantlets were successfully acclimatized (100%) and finally transferred to the greenhouse. Total phenolic content was measured from shoot, brown callus and white callus of L. barbarum L. grown in MS medium supplemented with auxin alone (for callus) and auxin-cytokinin (for shoots). The highest amount of total phenolic content (640 mg GAE/g DW) was obtained in shoot. The described protocol provides a simple way to regenerate plants through direct and indirect organogenesis as well as somatic embryogenesis, which would be useful for mass propagation, large-scale production of secondary metabolites, germplasm conservation, and genetic transformation studies in this medicinally important species.