Characterization of Ha29, a Specific Gene for Helicoverpa armigera Single-nucleocapsid Nucleopolyhedrovirus

Guo, Zhong-Jian,An, Shi-Heng,Wang, Dun,Liu, Yan-He,Kumar, V. Shyam,Zhang, Chuan-Xi Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.3

Open reading frame 29 (ha29) is a gene specific for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearSNPV). Sequence analyses showed that the transcription factor Tfb2 motif, bromodomain and Half-A-TPR (HAT) repeat were present at aa 66-82, 4-76, 55-90 of the Ha29 protein respectively. The product of Ha29 was detected in HearSNPV-infected HzAM1 cells at 3 h post-infection. Western blot analysis using a polyclonal antibody produced by immunizing a rabbit with purified GST-Ha29 fusion protein indicates that Ha29 is an early gene. The size of Ha29 product in infected HzAM1 cells was about 25 kDa, which was larger than the presumed size of 20.4 kDa. Tunicamycin treatment of HearSNPV-infected HzAM1 cells suggested that the Ha29 protein is N-glycosylated. Fluorescent confocal laser scanning microscope examination, and Western blot analysis of purified budded virus (BVs), occlusion-derived virus (ODVs), cell nuclear and cytoplasmic fraction, showed that the Ha29 protein was localized in the nucleus. Our results suggested that ha29 of HearSNPV encodes a non-structurally functional protein that may be associated with virus gene transcription in Helicoverpa hosts.

Business Blogging e-Hub: An Innovative Approach to e-Business

Guo-An Wang 한국통상정보학회 2005 통상정보연구 Vol.7 No.4

With the rapid development of the Internet, e-business has been entering a new phase. However, there’re still some problems to be settled and needs for breakthrough. The paper proposes an innovative approach to e-business models with the aim to resolve the problems. The paper reviews the exiting e-commerce models and points out some of the common weaknesses of the models, and presents the “Business Blogging e-Hub”model for e-business. With the characteristics of all-sided information, multi-dimensional interactivity and trans-model e-commerce platform, the proposed model is created and derived from the integration of the “classical”e-commerce models as B2B, B2C and C2C with the technology and essence of blogging, and is thus sure to meet rapidly changing business needs.

Piracy of China’s Trade Marks and Domain Names and Cultivation of its Famous Brands

Guo-an Wang 한국통상정보학회 2004 통상정보연구 Vol.6 No.2

A large number of Chinese trade marks and domain names have been registered improperly or illegally by foreign companies or businessmen. Additionally, a large number of famous Chinese brands have vanished through joint ventures, mergers and acquisitions since foreign direct investment (FDI) came to China more than two decades ago because some Chinese managers have not been fully aware of the value of trade marks and domain names. Consequently, the number of China’s registered trade marks and famous brands does not match China’s export volume and its No. four trade status in the world. China’s enterprises have yet to realize the effects of these events. It is very important for China to protect and cultivate its own famous brands. This paper discusses Chinese companies’ neglect of the value of their trade marks and domain names, and the possible consequences. Additionally, this paper puts forward suggestions concerning the protection and cultivation of China’s famous brands.

On China’s Intellectual Property Rights Protection Online

Guo-an WANG,Yong-taek LIM 한국통상정보학회 2004 통상정보연구 Vol.6 No.1

Online people conduct all kinds of business activities, such as promoting the sales of products and services, reading newspapers, watching TV and movies, and sending business messages by e-mail, by e-voice and by e-fax. Computers with access to the Internet are defined by the United Nations as the fourth media characterized by instantaneousness, openness, limitlessness, boundlessness and globalization. With the development and popularization of the Internet and the advance of the information technology in China, Intellectual Property Rights (IPRs) violations online have frequently occurred in e-business activities. IPRs protection online has posed a great challenge not only for business managers and officials, but also for judges and lawyers because the rapid development of the Internet has created a legal vacuum governed by no laws in IPRs violation and protection online in China.. The paper at first classifies IPRs into several categories, then reveals China’s serious problems and challenges of IPRs violations online and stresses the necessity of China’s IPRs protection online. Finally it puts forward some suggestions concerning IPRs protection online.

PLCE1 Gene in Esophageal Cancer and Interaction with Environmental Factors

Guo, Li-Yan,Zhang, Shen,Suo, Zhen,Yang, Chang-Shuang,Zhao, Xia,Zhang, Guo-An,Hu, Die,Ji, Xing-Zhao,Zhai, Min Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Objective: To study the PLCE1 gene rs2274223 polymorphism with regard to esophageal cancer and its interaction with diet, lifestyle, psychological and environmental factors in Southwest Shandong province. Materials and Methods: A case series study (case-case) was conducted. Questionnaire data were collected and 3 ml-5ml venous blood was drawn for DNA extraction among the qualified research subjects. PLCE1 gene polymorphism was detected after PCR amplification of DNA. SPSS 13.0 software was used for statistical analysis of the data. Results: The three genotypes A/A, A/G and G/G PLCE1 gene rs2274223 was 31, 16 and 4 cases, accounting for 60.8%, 31.4%, 0.08% respectively. The difference of three genotypes (AA/GA/GG) proportion between negative and positive family history of patients was statistically significant, ${\chi}^2=6.213$, p=0.045. There was no statistically significant relationship between PLCE1 gene rs2274223 polymorphism and smoking, drinking, ${\chi}^2=0.119$, p=0.998, and ${\chi}^2=1.727$, p=0.786. There was no linkage of the three rs2274223 PLCE1 gene genotypes (AA/GA/GG) proportion with eating fried, pickled, hot, mildew, overnight, smoked, excitant food, eat speed, salt taste or not (p>0.05). or with living environment pollution and nine risk factors of occupational exposure (p>0.05). There was no statistically significant difference in TS scores between different genotype of rs2274223 PLCE1 gene. Conclusions: The PLCE1 rs2274223 polymorphism has a relationship with family history of esophageal cancer, but does not have any significant association with age, gender, smoking, alcohol drinking, food hygiene, eating habits, living around the environment and occupation in cases.

Quality Control of Herbal Medicines and Their Related Products

Guo De-an 한국작물학회 2006 한국작물학회 학술발표대회 논문집 Vol.2006 No.-

On the Development of Alibaba.com

Guo-an Wang 한국통상정보학회 2002 통상정보연구 Vol.4 No.2

Alibaba.com has developed very fast and created a miracle in the international e-business community since it was established just three years ago. The paper at first introduces its rapid development from its establishment, the construction of its websites and its e-products and eservices, its mode of operation and corporate culture. Secondly it analyses the reasons why it has not only survived, but also developed very fast and begun to make profits despite the cut-throat competition in the e-business community in the tough e-environment in China. Then it displays and analyzes its competitive edges and the challenges it is confronted with. Finally it predicts its promising future.

On E-Commerce Development in Mainland China

Guo-an Wang,Yong T. Lim 국제e-비즈니스학회 2005 e-비즈니스 연구 Vol.6 No.3

Molecular Cloning, Characterization and Expression of a NovelTrehalose-6-phosphate Synthase Homologue from Ginkgo biloba

Guo-an Shen,Jin Wang,Kexuan Tang,Weisheng Wu,Yongzhen Pang,Jie Lu,Juan Lin,Xiaofen Sun 한국생화학분자생물학회 2006 BMB Reports Vol.39 No.2

On China’s Alibaba’s Success

Guo-An Wang,Xiaomei Qi,Shan Zheng 중앙대학교 한국전자무역연구소 2009 전자무역연구 Vol.7 No.3

지난 10년간의 개발기간동안 알리바바는 고유의 비즈니스 방식을 만들었는데 즉, 기업과 개인에게 시간과 공간의 단절 없이 운영하고 그 자체와 수백만의 중소기업들에게 전례가 없는 사업 기회를 제공하고 있는 국경 없는 전자무역 시장이다. 알리바바의 혁신적인 마케팅 전략은 그들의 개발을 가속화시켰고 세계의 전자상거래 분야에서 두드러졌다. 이 논문은 알리바바의 성공 경로에 대해 처음으로 연구되는 것이며 McCarthy 교수의 4Ps 이론에 기초한 효과적인 마케팅 전략에 대해 살펴본다. 마지막으로 2008년에 있었던 세계 금융 위기에 맞선 마케팅 전략의 향상에 관한 제안을 제공한다. During the 10 years of development, Alibaba has established a unique business mode, a borderless e-trade market running around the clock on the Internet for enterprises and individuals without limit in time and space and providing itself and millions of small and medium-sized enterprises(SME) with unprecedented business opportunities. The innovative marketing strategies of Alibaba has accelerated its development and distinguished itself in the field of e-commerce in the world. This paper at first offers an overview of Alibaba’s path of development, and then discusses its effective marketing strategies based on Professor McCarthy’s 4Ps theory. Finally it provides some suggestions on the improvement of its marketing strategies regarding the fight against the financial crisis worldwide which began in 2008.