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Meltem Asan-Ozusaglam,Ayse Gunyakti 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.2
The study aimed to identify two lactic acidbacteria from human breast milk and evaluate for theirprobiotic potential. Lactobacillus fermentum strainsshowed various degree of antibiotic susceptibility profile toclinical commercial antibiotics. The MA-7 and MA-8strains have never lost their vitality at pH 2 and 3. Lactobacillusfermentum MA-8 exhibited more resistance to 0.3and 1% bile concentrations than MA-7 strain. Both of theL. fermentum strains can tolerate the simulated gastric andintestinal juices. The strains showed high auto-aggregationpercentages varied from 95 to 98%. Both isolates alsoexhibited gamma hemolytic activity. The cholesterol loweringactivity of MA-7 and MA-8 strains was varied from34.84 to 91.15%. The supernatants showed higher anticholesterolactivity compared to pellets. The resultsdemonstrated that both L. fermentum strains maybe goodprobiotic candidates and may have potential as healthbiotherapeutic with cholesterol-lowering effects.
Haroon Ahmed,Sérgio Ramalho Sousa,sami simsek,Sofia Anastácio,Seyma Gunyakti Kilinc 대한기생충학ㆍ열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.6
Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The objective of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.