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Gung Pyo Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Recently, many breeders have preferred to use molecular markers for introgression backcross programs enabling foreground and background selection to cope with rapid cultivar changing of seed markets. In accumulation of target traits with marker-assisted selection, larger numbers of markers should give better resolution. For the analysis of quantitative traits, a high-density genetic map with a large number of markers is required for discovering more accurately linked markers with traits. Watermelon is a recalcitrant plant to generate a high-density genetic map with conventional molecular markers including simple sequence repeats (SSRs), since watermelon has narrow genetic diversity background and severe segregation distortions of those SSR markers. Thus, we have developed efficient and valid way to assemble genetic map and markers by next-generation sequencing coupled with genotyping by sequencing in F2 generation. After crosses between Citurullus lanatus ssp. citroides (PI254744 and PI189225) and C. lanatus ssp. lanatus (TS34, Korean cultigen), 163 of F2 progeny were sequenced through Illumina's Hi-Seq GAII platform. From sequence information of those variant call files, the SNPs were indexed and filtered by sequencing depth with genotype converter (SNP Genotyper), and optimized by heuristic physical bin mapping to construct more reliable genetic linkage map. Reliable SNP loci were determined and compared to sequences of physical reference map. Using the genetic map, we determined QTLs in F2:3 population and found major loci corresponding to seed size and powdery mildew race1 resistance in watermelon.
Effect of GeO₂ Concentration on Hydroponically-Grown Lettuce (Lactuca sativa)
Gung Pyo Lee,Hee-Seung Park,Jae Hee Won,Kuen Woo Park 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.2
Various concentrations of germanium dioxide (GeO₂) was treated such as 1.0, 2.0, 4.0, 6.0, and 8.0 ㎎ㆍL?¹ to lettuce (Lactuca sativa cv. Cheongchima) cultured in hydoponics for 4 weeks. Ge was linearly accumulated in the plants, primarily in the roots, as GeO₂ concentration in the nutrient solution increased from 1.0 to 8.0 ㎎ㆍL?¹. In addition, Ge once taken by the root was hardly moved to the shoot. The plant showed the best growth at 4.0 ㎎ㆍL?¹ GeO₂ for 7 days. In all ranges of the Ge treatment, the plant showed no phenotypic changes in leaf shape and color including necrosis or chlorosis, but the roots were stunted and thickened. The roots treated with GeO₂ at 6 or 8 ㎎ㆍL?¹ showed thickened cortex, but they had similar sizes of cells and vascular tissues. The leaf tissues treated with GeO₂ had denser mesophyll cells than the control. Interestingly, the Ge-treated leaf looked harder, more greenish, and more glossy than the control. With increasing GeO₂ concentration in the nutrient solution, the root activity was decreased, whereas lipid peroxidation increased.
Factors Affecting on Regeneration in 'WHANGKEUMBAE' Pear (Pyrus pyrifolia)
Gung-Pyo Lee 경상대학교 농업생명과학연구원 2009 농업생명과학연구 Vol.43 No.6
Combinations of plant growth regulators, darkness treatments, and the order of expanding leaves for explants were evaluated for optimizing in vitro shoot regeneration rate of 'Whangkeumbae' pear. In a MS medium, supplemented with 8.88 μM 6-benzylaminopurine (BA) and 0.49 μM indole-3-butyric acid (IBA), green foci were observed on the surface of the callus 8 days after culture initiation. Some adventitious buds were later induced from those green foci, resulting in the appearance of normal shoots. In a medium containing 22.20 μM BA, the surface of the callus became compact and greenish, and many adventitious buds were formed over the entire area of the callus surface. When comparing BA concentration via histological observation, the section which had been treated with 22.20 μM BA exhibited closer cell aggregation than those with 8.88 μM BA. The darkness treatment enhanced the formation of adventitious shoots for up to 3 weeks. The youngest two expanding leaves, proximal to the shoot apex, were proved to be the most regenerative, and yielded the highest shoot number per regenerating leaf. A fourth strength MS medium, which was supplemented with 0.54 μM NAA, yielded good quality plantlets, with regard to root number and root length.