사람 혈청 알부민에 대한 단세포군 항체의 제조와 특성 연구

태건식,안혜진 호서대학교 반도체제조장비국산화연구센터 2000 학술대회 자료집 Vol.2000 No.1

Serum albumin은 혈청 단백질 중 50-60%를 차지한다. 알부민은 순환계 내에서 삼투압을 유지시켜 세포내외의 체액을 유지 및 지용성 물질의 이동에 관여한다. 신사구체와 기저막의 투과성이 증가하거나 세뇨관의 흡수감소 등으로 단백질의 요배설량이 하루에 150mmg 이상되는 단백뇨는 신장내의 병변이 있음을 나타내는 중요한 지표이다. 이러한 단백뇨는 크게 사구체성 단백뇨, 세뇨관성 단백뇨, 혼합성 단백뇨로 나눌수 있는데 이중 사구체 기저막의 손상으로 발생되는 사구체성 단백뇨는 알부민과 같은 고분자량의 단백질이 소변 내로 배설되는 현상이다. 일반적으로 알부민이 정상치 이상으로 소변을 통하여 배설되는 것을 'microalbuminuria'라고 하며 당뇨병으로 인한 신장병변을 예견하는데 중요한 지표로 알려져 있다. Microalbuminuria는 방사 면역확산법, 방사성 면역측정법, 면역혼탁측정법, 비탁측정법, 효소결합면역측정법(ELISA) 등으로 겸출할 수 있으나 최근에는 안전하면서도 민감도가 높고 측정이 용이한 방법인 ELISA를 이용한 면역분석방법이 많이 사용되고 있다. 면역특이성이 떨어지는 다세포군 항체를 사용한 방법의 문제점을 극복하기 위해서 세포융합기술을 이용한 단세포군항체를 생산하는 세포주를 개발하였고 그 특성을 규명하였다. 따라서 본 연구에서 세포융합기술을 도입하여 개발된 인간혈청 알부민에 대한 단세포군항체는 glycohemoglobin에 대한 단세포군항체와 더불어 당뇨병의 진행 정도를 진단할 수 있는 키트의 원료로 사용될 수 있다.

담배거세미나방에서 분리된 항균펩티드 유전자의 대량 발현에 관한 연구

태건식,현석진 단국대학교 1999 論文集 Vol.34 No.-

The antibacterial gene isolated from the common cutworm. Spotoptera litura showed high degree of homologies in its nucleotide sequence to those of the cecropin genes from Hyalophora cecropia, implying that it may encode the antibacterial peptide. In this experiment, the antibacterial gene from S. litura was subcloned to the expression vector pGEX-1λT and overexpressed in BL21. The antibacterial gene is composed of total 114 nucleotides which encodes the relatively small peptide of 37 amino acid residues. This gene was designed to make a fusion protein with glutathione-S-transferase in response to the addition of IPTG. The total proteins from the cell extract were separated by SDS-PAGE and the overexpressed fusion protein was detected at the right positon in terms of its molecular weight. The fusion protein was also purified by use f the affinity column chromatography technique with the resin in which glutathione was cross-linked to 4% agarose bead.

팔씨름 중 발생된 상완골의 나비형 골편을 동반한 분쇄형 나선 골절

이근우,손정환,김태훈 고신대학교의과대학 2012 고신대학교 의과대학 학술지 Vol.27 No.1

The fracture of the humerus during arm wrestling are uncommon, but may occur in the shaft or medial condyle. In the shaft of humerus, the fractures are usually spiral and between the middle and distal third, with or without neurological involvement. A case of a comminuted spiral fracture with butterfly fragment of the distal third of the humerus is reported which had occurred during arm wrestling and was treated by open reduction and internal fixation. The possible mechanism of injury and the question of treatment are discussed.

IC 실현에 적합한 정현파 능동 발진기의 회로 및 특성에 관한 연구(Ⅱ)

이원건,손태호,박종연 江原大學校 産業技術硏究所 1991 産業技術硏究 Vol.11 No.-

Two kinds of simple active oscillators are proposed and analyzed assuming that operational amplifier has two-poles frequency characteristics. The first circuit is composed of one operational amplifier, one resistor and one grounded capacitor. The second oscillator is realized with one operational amplifier and three resitors. Proposed oscillators have the low sensitivity of the oscillation frequency for little variations of the passive element values. By the experimental results obtained with Op-Amp.?A741, the simple oscillators can be useful for the frequency range 1.25 KHz≤f? ≤400KHz for the active -R ocillator, and it is shown to transform the active-R oscillator circuit into the voltage controlled type.Therefore, two kinds of oscillators circuit are attractive for the IC realization, because they hve one operational amplifier, one resistor and one grounded capacitor, or three resistors.

열대열 말라리아 치료 후 발병한 삼일열 말라리아 1예

이건화,김태형,박노진 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Endemic malaria in the northen parts of the Republic of Korea is caused by P. vivax. And imported malaria cases in Korea from south Asia, Indian subcontinents are usually caused by P. falciparum species. We report a case P. vivax malaria after successful treatment of P falciparum malaria with a review of literatures. Co-infection of P. vivax and P. falciparum, which relapsed only with P. vivax, after successful treatment of P. falciparum malaria was considered. A 38-year old Bangladeshi woman who had lived in Korea visited our hospital due to fever and headache. Eight months ago, she was admitted to a hospital in Calcutta in India because of P. falciparum malaria. She was treated successfully with mefloquine and doxycycline. In this time she was presented with features of malaria with every-other day fever. Awaiting the species identification, we empirically prescribed mefloquine and doxycycline. But the peripheral blood smear showed erythrocytic schizogony, trophozoite and gametocytes typical of P. vivax. So she was finally treated with hydroxychloroquine and primaquine. The patients was well during outpatient follow up period.

요추간판 탈출증 환자의 수술전,후 및 안정 회복에 따른 요부 등척성 신근력의 변화

윤규태,김건도,한길수 대한스포츠의학회 2003 대한스포츠의학회지 Vol.21 No.1

The purpose of this study was to determine the quantitative assessment of lumbar extension strength change for pre-operation, post-operation and following 4 week rest in hernia-operated patients. Twenty-four(11 males and 13 female) subjects participated and was tested at 3 times for pre-operation, post-operation and following 4 week rest. Each test involved the measurement of maximum voluntary isometric torque at seven degrees(72˚, 60˚, 48˚, 36˚, 24˚, 12˚, 0˚) of lumbar flexion using the Medx lumbar extension machine. There was no significant improvement of lumbar extenstion strength between pre-operation and post-operation for male at all degrees but for female significant increase was appeared at major degrees except 48˚, 0˚ between pre-operation and post-operation and at all degrees between pre-operation and following 4 week rest. Result further on body weight ratio show same results except that there was significant increase for female at all degree except 0˚ between pre-operation and post-operation.

부산지역 국지기후의 특성분석

김유근,서태건,송상근,오인보 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-

This study presented the specific analysis of meteorological factors to investigate local climate change of Busan and deduced the characteristics of meteorological elements such as monthly temperature, relative humidity, wind, and precipitation by the influence of urbanization and geographical features. This study used data obtained from Busan Regional Meteorological Office and Automatic Weather System (AWS) for the recent 30 years (1971 to 2000). In analyzing meteorological elements, air temperature, precipitation and hours sunshine show a slightly increasing trend, and wind speed, relative humidity, cloud amount, and radiation show a decreasing trend. A special point is that increasing radio of maximum temperature is higher than that of minimum temperature, then the daily range of temperature is gradually increasing. In addition, the characteristics of local climate vary as each area in Busan. At Seomyeon and Dongnae area which are located at the center of Busan, heat island phenomena occurred over all seasons, especially its intensity is the strongest in August. The annual precipitation is highest at Youngdo, Daecheongdong, Ilgwang which are located at the seaside. Wind velocity is relatively strong at the southern seaside.

CO₂의 메탄화 반응에서의 Ni촉매의 활성도와 선택도

이태진,이원근,김장희 嶺南大學校 工業技術硏究所 1988 연구보고 Vol.16 No.1

The reactivity and selectivity of CO₂methanation over Ni/SiO₂ catalyst was investigatd with process variables(temperature, time factor, and H₂/CO₂ ratio). Apparet activation energies for CH₄ formation were 21.3㎉/mol and 25.8Kcal/mol in CO₂ and CO methanation, respectively. Besides CH₄, small amounts of CO were also formed with activation energy 19.4㎉/mol for CO formation. The rate of CH₄formation and outlet CO concentration were nearly independent of space time at any given temperature and H₂/CO₂ratio. The Selectivity to CH₄and outlet CH₄concentration, however, increased with increasing space time. These results suggest that the methanation of CO₂is series reaction involving CO formation. Results show that CH₄ production increases at the expense of CO production with increasing temperature, space time, and H₂/CO₂ratio.

지급정책의 결정요인과 성과에 관한 연구

김태근 원광대학교 대학원 2009 論文集 Vol.42 No.-

The growth of foreigner's investment into Korean capital Market has brought the change of corporate ownership structure, and the abolition of stock repurchase limit has provided a new environment of decision making on payout policy of Korean corporate. Given the changed environments, this study attempted to find the relationships between corporate ownership and payout policy with financial structure of corporate and between payout policy and the change of corporate market value. It performed an empirical study on the 2002-2007 data of companies which had simultaneously done the repurchase of their stocks and the dividends policy. As the results, ROA and M/B have a significant relationship with dividends policy, and ROA and DEBT with stock repurchase. And stock repurchase has a positive relationship with dividends policy and has a significant negative relationship with the change of stock market value.

유사접촉 HDD 슬라이더 공기베어링의 부상상태에 관한 연구

정태건 건국대학교 산업기술연구원 2001 건국기술연구논문지 Vol.26 No.-

The progress of head/media technology is directly related to the reliability of the HDD. The most fundamental data in the design of HDD slide air bearing is the accurate estimates of flying height and air bearing stiffness. The numerical analysis of the generalized Reynolds equation provides the useful results consistent with the various experiments. The flying characteristics in operating condition such as track seek, however, are difficult to measure and predict accurately. The measurement of the flying height usually needs a flying height tester that is only for the static measurement. In this study, we develop the AE measurement system to measure the air bearing characteristics in various operating conditions Indirectly. The AE sensor can measure the flying characteristics with slider/disk contacts, which leads to the head/media interface study. We can study the boundary lubrication states by measuring the AE signal for various disk rotation speeds.