구강균에 대한 각종 한약제의 증식 억제

정영건,지원대,김성영,곽동주,서수교,백경연 한국위생과학회 1997 한국위생과학회지 Vol.3 No.1

The effect of oriental medicines an the growth of oral bacteria(B. pumilus JU4, B. coagulans JU6, B. subtilis var globigii JU7 and B. subtilis var. globigii JU9) which were isolated from teeth, gums and tongues of patients was investigated. lt was shown that the water extracts of Glycyrrhiza glabra L. var. glandulifera Regel et Zucc., Schizandrae Fructus and Coptidis Rhizoma have the strong antimicrobial activity against every test organism. Especially, the water extract of Schizandrae Fructus has the most antimicrobial activity against all test bacteria of which MIC were the same as 0.25%. The growth of oral bacteria B. pumilus JU4 was inhibited by addition of the water extracts of Glycyrrhizae Radix, Schizandrae Fructus and Coptidis Rhizoma in the concentration of 0.9%, 0.2% and 0.1% respectively. It was tested that specific growth rates of B. pumilus JU4 in each concentration of the water extracts of Glycyrrhizae Radix, Schizandrae Fructus and Coptidis Rhizoma. From the results, the test showed that mean of specific growth rates of B. pumilus JU4 decreased 102%, 186% and 210% in 3% concentration of the water extracts of Glycyrrhizae Radix, Schizandrae Fructus and Coptidis Rhizoma more than those in control respectively.

Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 세신 추출액의 영향

정영건,권오진,윤수홍,이송애,이주영 효성여자대학교 부설 한국환경위생연구소 1993 환경위생연구 Vol.3 No.1

탄소화합물의 불완전 연소 및 열분해에 의해 생성되는 다환방향족 탄화수소로 간 실질세포에서 cytochrome P_450에 의해 산화되는 간장해 물질인 benzo(a)pyrene으로 유도한 rats의간독성 발현에 미치는 세신 수침액의 예방 및 치료 효과를 실험한 결과 세신 수침액의 투여는B(a)P 투여로 현저하게 증가된 혈청 및 간장의 transaminase 활성을 유의성 있게 감소시켰으며 그효과는 조직에서 보다 우세하였다. 그러나 세신 수침액이 B(a)P투여로 증가한혈청 total cholesterol과 phospholipid 함량을 감소시킬 수는 있었지만 그 효과는 약하여 지질대사에는 세신의 작용이 미미할 것으로 보인다. Effects of Asiasari Radix, which is herbal drug used frequentlyin the oriental prescriptions, water extract on the liver-protective activities wereinvestigated in the rats. Asiasari Radix water extract, when administered intothe gastric intubation, produced liver-protective effect against benzo(a)pyreneinduced liver damage. The results obtained from enzyme assay, measurement of serum and liveralanine aspartate aminotransferase(AST, ALT) and lipid composition indicatedthat Asiasari water extract showed significant liver-protective activities againstbenzo(a)pyrene-induce hepatotoxicity.

Cephalosporium acremonium var. M-10에 의한 Cephalosporin C 생산에 관한 연구

이호근,현형환,오두환,유주현 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.2

The microorganism which produces Cepholsoporin C, has been selected from Cephalosporium acromonium by U.V radiation. Among the mutants, Cephalosporium acremonium var. M-10 was showed the highest Cephalosporin C productivity. On the basis of physiologieal characteristics, the difference hetween Cephalosporium acremonium var. M-10 and Cephalosporium acromonium was that the mutant did not assimilate sodium acetate, potassium nitrate, sodium nitrate. By using Cephalosporium acremonium var. M-10, the optimum conditions for the production of cephalosporin C on the rotary shaker were pH 7.0 soluble starch 4.0 w/v%, (NH₄)₂SO₄0.75w/v%,, CaCl ₂w/v%, soy bean meal 2w/v%, oleic acid 0.09w/v%, methionine 0.3w/v%, at 30℃ for 120 hours, respectively. On the study of time course, it revealed that the peak appeared at the stationery phese. The amount of Cephalosgorin C produced in this medium by mutant was about two times larger than that by Cephalosporium acremonium.

三姓神話의 形成과 文獻定着過程

張籌根 제주대학교 탐라문화연구소 1994 탐라문화 Vol.0 No.14

단조강(SF45A)재의 표면피로크랙 성장 거동에 관한 고찰

金健鎬,정재강,최종로,방웅주 군산대학교 1993 群山水産專門大學 硏究報告 Vol.28 No.1

Fatigue life prediction of fatigue fracture is very important problem. This paper is observed on the surface small crack growth behaviour of smooth specimen. This results were as follows. Surface small crack are created small crack of high density in about 50% of fatigue life, retarded crack growth due to crack coalescence in about 60% and 85%, and when distributed small cracks are growing, it was growthed few large cracks. On the other hand, To analyze crack growth rate used stress intensity factor was difficult due to existed small crack stegnant range.

중증 근무력증 52례에 대한 임상적 고찰

박건주,하정상,이준,도현철,박승권,서상덕,기병수,김진국 영남대학교 의과대학 1996 Yeungnam University Journal of Medicine Vol.13 No.1

1985년 8월부터 1996년 1월까지 중증근무력증으로 진단받은 환자 52례에 대한 임상적 고찰을 통하여 다음과 같은 결론을 얻었다. 1. 전체 환자 중 남자가 19례(35.8%), 여자가 33례(64.2%)이넜고 11세에서 20세 사이가 12례(23%)로 가장 높은 빈도를 보였다. 2. 환자의 초기증상으로는 안검하수, 복시 등의 안구증상이 37례(71.2%)로 가장 많았고 modified Osserman의 분류에 따라서도 가장 증상이 가볍고 약물치료에 잘 반응하는 1군이 36례(69.3%)로 가장 많았다. 3. 환자가 호소하는 증상의 비특이성과 회복과 재발을 거듭하는 질병의 특성으로 인해 발병이후 진단될 때까지의 시간은 비교적 오래 걸렸고 1년이상 경과하며 진단된 경우도 16례(30.8%)나 되었다. 4. 자가면역질환이 7.6%에서 동반되었는데 갑상선기능 항진증이 2례(3.8), 인슐린의존형 당뇨 2례(3.8%)였고 종격동 전산화 단층촬영소견에서 흉선의 비대가 의심되었던 환자는 6례(11.5%), 흉선종이 발견 되었던 경우는 3례였다. 5. 환자의 치료는 대부분 항콜린에스테라제나 부신피질호르몬을 병용하는 내과적 치료를 하였으며 약물의 치료에 반응이 미비하거나 악화되는 경우 혹은 종격동 전산화 단층촬영상에서 흉선의 이상 소견이 보인 경우에 2례(3.9%)에서는 흉선절제술이, 1례(1.9%)에서는 혈장여과요법이 추가 되었고 흉선절제술과 혈장여과요법이 같이 병합 치료된 경우도 3례(5.9%)있었다. 6. 전체 환자중 2명에서 자연치유의 경과를 보였으며 이들은 모두 1군이였다. 그리고 흉선절제술과 부신피질 호르몬 투여로 완치된 예가 1례있었다. The authors experienced 52 patients with myasthenia gravis who were diagnosed at the Department of Neurology, Yeungnam University Hospital from August 1985 to January 1996. The following results were obtained through diagnostic evaluation and treatment. 1. The ratio of male to female was 1:1.7 and the most prevalent age group was second decade. 2. The most common initial presentation symptom was ocular(71.2%) and the peak incidence group was stage I(69.3%) according to the modified Osserman's classification. 3. In 16 patients(30.8%), it took more than a year to diagnose due to symptoms which were relapsed and remitting. 4. Of 52 patients, 2 cases were associated with thyroid disease(3.8%) and 2 with insulin-dependent diabetes mellitus(3.8%). 5. All of those who received anticholinesterase and corticosteroid therapy were improved with the exception of 5 cases which were improved after thymectomy and/or plasmapheresis. 6. Spontaneous remission was occured in 2 cases(3.8%) of ocular myasthenia gravis. And 1 case of group II was cured through thymectomy and long-term corticosteroid therapy.

莞島近海의 海洋環境에 관한 硏究

曺炷煥,金成根 조선대학교 기초과학연구소 1994 自然科學硏究 Vol.17 No.1

The results from investigation for the physical, chemical and biological marine environments during the period from February 1989 to November 1990 in the Wando coastal areas are as follows: 1. The water temperature appeared high in proportion to a amount of diural sun rays and air temperature. 2. The salinity appeared differently every season by a amount of precipitation. 3. The distribution of pH was settled, and the degree of acidity apperared relatively high in the area where the river waters flows. 4. The dissolved oxygen was low in summer season, but high in winter. 5. The distribution of the chemical oxygen demand was distributed irregularly every season. 6. The suspended materials appeared remarkably high on St.1 in 1990. 7. The concentration of the phosphate-phosphorous is higher in river waters than in sea waters, and the phosphate-phosphorous in sea waters was mostly flowed from lands. 8. The concentration of the dissolved inorganic nitrogen appeared high in autumn when nitrite-nitrogen and nitrate-nitrogen is more concentrated. 9. The relative relationship between phosphate-phosphorous and dissolved inorganic nitrogen appeared higher comparatively in 1990 than in 1989. After all. our investigation suggests that the physical, chemical character brings about an abnormal variation of the marine ecosystem, and obstructs the birth and breeding of the submarine livings. Therefore, we must draw up divers shemes for the preservation of the quality of coast water, and concentrate our efforts on doing away with factors of all marine pollution which spread rapidly in proportion to the industrial development.

W/O 마이크로에멀젼에 의한 나노크기의 TiO₂/SiO₂ 합성에서 물/계면활성제의 몰 비(W_(0)) 영향 및 그 광분해 특성

이만식,주창식,이근대,홍성수 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.4

나노크기의 TiO₂/SiO₂입자는 음이온 계면활성제 AOT(sodium bis(2-ethylhexyl) sulfosuccinate)를 사용하여 W/O 마이크로 에멀젼에서 TTIP(titanium isopropoxide)와 TEOS(tetraethylorthosilicate)의 가수분해 반응에 의해 제조하였다. 그때 TEOS의 몰분율은 0.1이였다. 나노입자 제조시 W_(0)(H₂O/AOT)비에 따른 열적안정성, 표면적, 결정성 및 결정크기 등과 같은 물리적 특성은 TEM, XRD, BET, FT-IR, TGA-DTA 등을 사용하여 분석하였다. 또한 광촉매적 특성을 알아보기 위해 회분식 반응장치를 이용하여 p-니트로페놀의 광분해 활성을 조사하였다. 제조된 Ti0₂/SiO₂ 나노입자는 열처리 온도 105℃, 300℃에서 비결정구조를 가졌으며, 소성온도 800℃에서는 Ti0₂ 결정입자 내에 무정형의 SiO₂로 인해 열적 안정성이 증가되었고, 여전히 anatase 결정을 유지하였다. 그리고 Ti0₂/SiO₂(90/10) 입자에서 SiO₂ 결정은 관찰되지 않았다. 제조된 촉매는 대체로 구형이며 아주 균일한 분포를 갖는 것으로 관찰되었다. W_(0) 비가 증가할수록 결정크기는 증가되었고, 표면적은 감소하는 경향을 나타내었다. 또한 p-니트로페놀에 대한 광분해 활성은 순수한 Ti0₂ 보다 Ti0₂/SiO₂(90/10) 촉매가 우수하였다. Nanosized TiO₂/SiO₂ particles were prepared by hydrolysis of TTIP (titanium isopropoxide) and TEOT (tetra-ethylorthosilicate) in sodium bis (₂-ethylhexyl)sulfosuccinate (AOT) reverse micelles. The mole fraction of TEOS was 0.1. The physical properties, such as surface area. thermal stability, crystallite size and crystallinity according to W_(0), ratio have been investigated by TEM, XRD, BET, FT-IR, TGA and DTA. In addition. the photocatalytic degradation of p-nitrophenol has been studied by using batch reactor in order to compare the photocatalytic activity of prepared nanosized TiO₂/SiO₂, particles. It is shown that the XRD pattern of the particle heat treated at 105℃ and 300℃ indicates amorphous and the major phase of all the prepared particles were anatase structure. No significant rutile phase was observed although the calcination temperature at 800℃ and no peaks SiO₂ crystal were also observed for TiO₂/SiO₂ (90/10). The presence of amorphous SiO₂ in TiO₂/SiO₂ particle enhanced the thermal stability of TiOz particle resulting in the suppression of the phase transformation from anatase to rutile phase. The crystallite size of prepared particles decreased with decreasing W_(0) ratio. The surface area increased with decreasing W_(0) ratio. In addition, TiO₂/SiO₂ (90/10) particles shows higher photoactivity than that of pure TiO₂ particles.

상엽 추출물을 이용한 항당뇨 음료의 개발(Ⅰ) : 상엽 추출물의 제제화 탐색 Screening of the Manufacturing of Mulberry Leaf Extract

구성자,윤기주,김근풍 동아시아식생활학회 2002 동아시아식생활학회지 Vol.12 No.5

Mulberry leaves arc believed to be a potent inhibitor of intestinal α-glycosidase and the digestion of sucrose in the small intestine. Mulberry leaves are also known to help prevent the postprandial hyperglycemia. The objective of this study was to elucidate the functionality and anti-hyperglycemic effect of mulberry leaves, and to develop a functional drink using mulberry leaf, silk peptide and oriental medicine. Several mixtures of mulberry leaf extract, silk peptide and oriental medicine were made for the purpose of manufacture and formulation of products. These mixtures were tested to check the α-glycosidase inhibition effect to find the best formula. The optimum conditions for a mulberry leaf hot water extract were that the size of leaf was under 3㎜, the amount of leaf needed 50~100 g/L, extraction temperature 90℃ and extraction time 2 hr. The yield of extraction was 20~25%. The anti-hyperglycemic effect of mulberry leaves was at maximum when the concentrations of mulberry leaf extract and silk peptide were 1% and 0.1% respectively.

생쥐 위장관의 Interstitial cells of Cajal(ICC)에 대한 면역조직화학적 연구

김영철,차경훈,신무경,임건한,김주영,안병수,김장만,양경철,박도영,오재욱,장인엽 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Background and Objectives : Interstitial cells of Cajal(ICC) are the pacemakers in gastrointestinal tract that modulates gastrointestinal motiliey and these cells also transmit neural input from enteric nerves to smooth muscles. Recent work on tissues from patients with motility disorders that suggest that loss or defect in ICC could be related to pathophysiology in human and animal models. Immunolabelling of ICC in intestinal wall is recently developed by using specific marker, anti-c-kit antibody. Immunohistochemistry was done for ICC network in attempt to provide a morphological basis for the mechanism regulating gastrointestinal motility Methods : Cryosection was done, and whole-mount preparations of mouse stomach, gastrointestinal tract were immunolabelled using the anti-c-Kit. Immunolabelled ICC networks were observed under a confocal laser scanning microscopy. Results : According to three dimensional reconstruction study, we found that the c-Kit-positive celluar networks were widely distributed in the gastrointestinal muscle (1) circular muscle layer(IC-IM), (2) myenteric plexus(IC-MY), (3) deep muscular plexus(IC-DMP) in ileum, (4) submucosal plexus(IC-SMP) and longitudinal muscle layer(IC-LM) in colon. Conclusion : The characteristic profiles of ICC celluar networks provide a morphological basis upon the mechanism regulating gastrointestinal motility. Additional studies for the enteric nerves-ICC interaction are need to evaluate the detailed roles of Icc in gastrointestinal tract.