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Metric Scale Study of the Bonded Concrete-Rock Interface Shear Behaviour
Marion Bost,Hussein Mouzannara,Fabrice Rojat,Grégory Coubard,Jean-Pierre Rajot 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.2
The shear strength of concrete-rock interface is a key factor to evaluate the stability of gravity dams. The shear strength assessment by achieving tests on small samples gives values different from those estimated by back-analysis on the existing dams. This work aims to study the shear behaviour of concrete-rock interface in the metric scale. Five direct shear tests were performed on bonded meter-scale concrete-granite interfaces in the range of normal stresses to which gravity dam foundation is subjected. Specific instrumentation were installed to monitor the failure mechanisms during the tests. The five concrete-rock interfaces have not broken by shearing of materials (concrete, rock) in the shear plane imposed by the test device, but by debonding of the contact between concrete and rock. Considering roughness of the contact surface in the decimeter scale and the results of shear tests carried out in the same scale, the decimeter scale is demonstrated to correspond to the elementary surface for the shear behaviour of the metric concrete-rock interface. According to the level of normal stress, the stiffness of both materials and the main asperities in the decimeter scale, different failure mechanisms occur locally to justify the overall failure in the metric scale.
Jérôme Mounier,Geneviève Héry-Arnaud,Audrey Gouëllo,Marlène Keravec,Solène Le Gal,Grégory Pacini,Stella Debaets,Gilles Nevez,Gilles Rault,Georges Barbier 한국미생물학회 2014 The journal of microbiology Vol.52 No.4
The aim of this study was to evaluate the use of denaturinghigh-performance liquid chromatography (DHPLC) to characterizecystic fibrosis (CF) airway microbiota includingboth bacteria and fungi. DHPLC conditions were first optimizedusing a mixture of V6, V7 and V8 region 16S rRNAgene PCR amplicons from 18 bacterial species commonlyfound in CF patients. Then, the microbial diversity of 4 sputumsamples from 4 CF patients was analyzed using culturalmethods, cloning/sequencing (for bacteria only) and DHPLCpeak fraction collection/sequencing. DHPLC analysis allowedidentifying more bacterial and fungal species than the classicalculture methods, including well-recognized pathogenssuch as Pseudomonas aeruginosa. Even if a lower number ofbacterial Operational Taxonomic Units (OTUs) was identifiedby DHPLC, it allowed to find OTUs unidentified bycloning/sequencing. The combination of both techniquespermitted to correlate the majority of DHPLC peaks to definedOTUs. Finally, although Aspergillus fumigatus detectionusing DHPLC can still be improved, this techniqueclearly allowed to identify a higher number of fungal speciesversus classical culture-based methods. To conclude, DHPLCprovided meaningful additional data concerning pathogenicbacteria and fungi as well as fastidious microorganisms presentwithin the CF respiratory tract. DHPLC can be consideredas a complementary technique to culture-dependentanalyses in routine microbiological laboratories.
Extracellular vesicle-based therapeutics: natural versus engineered targeting and trafficking
Daniel E. Murphy,Olivier G. de Jong,Maarten Brouwer,Matthew J. Wood,Grégory Lavieu,Raymond M. Schiffelers,Pieter Vader 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Extracellular vesicles (EVs) are increasingly being recognized as mediators of intercellular signaling via the delivery of effector molecules. Interestingly, certain types of EVs are also capable of inducing therapeutic responses. For these reasons, the therapeutic potential of EVs is a topic of intense research, both in the context of drug delivery and regenerative medicine. However, to fully utilize EVs for therapeutic purposes, an improved understanding of the mechanisms by which they function would be highly advantageous. Here, the current state of knowledge regarding the cellular uptake and trafficking of EVs is reviewed, along with a consideration of how these pathways potentially influence the functions of therapeutic EVs. Furthermore, the natural cell-targeting abilities, biodistribution profiles, and pharmacokinetics of exogenously administered EVs, along with the components responsible for these features are discussed. An overview of the potential clinical applications and preclinical examples of their successful use is also provided. Finally, examples of EV modifications that have successfully been employed to improve their therapeutic characteristics receive a particular focus. We suggest that, in addition to investigation of EV cell targeting and routes of uptake, future research into the routes of intracellular trafficking in recipient cells is required to optimally utilize EVs for therapeutic purposes.
A discontinuous Galerkin method for elliptic interface problems with application to electroporation
Guyomarc'h, Gré,gory,Lee, Chang-Ock,Jeon, Kiwan John Wiley Sons, Ltd. 2009 Communications in numerical methods in engineering Vol.25 No.10
<P>We solve elliptic interface problems using a discontinuous Galerkin (DG) method, for which discontinuities in the solution and in its normal derivatives are prescribed on an interface inside the domain. Standard ways to solve interface problems with finite element methods consist in enforcing the prescribed discontinuity of the solution in the finite element space. Here, we show that the DG method provides a natural framework to enforce both discontinuities weakly in the DG formulation, provided the triangulation of the domain is fitted to the interface. The resulting discretization leads to a symmetric system that can be efficiently solved with standard algorithms. The method is shown to be optimally convergent in the L<SUP>2</SUP>-norm. We apply our method to the numerical study of electroporation, a widely used medical technique with applications to gene therapy and cancer treatment. Mathematical models of electroporation involve elliptic problems with dynamic interface conditions. We discretize such problems into a sequence of elliptic interface problems that can be solved by our method. We obtain numerical results that agree with known exact solutions. Copyright © 2008 John Wiley & Sons, Ltd.</P>