http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Sergio Carvalho de Santana,Everaldo Silvino dos Santos,Raimundo Cosme da Silva Filho,Jorge dos Santos Cavalcanti,Jackson Araujo de Oliveira,Gorete Ribeiro de Macedo,Francine Ferreira Padilha 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.4
A mathematical model was developed to predict breakthrough curves during purification of the two chitosanasesfrom Metarhizium anisopliae by expanded bed adsorption, taking into account the axial dispersion of liquid andusing Streamline DEAE and SP XL adsorbents, anion and cation exchange resins, respectively. All the experimentswere performed without clarification (with cells) aiming at the reduction of unit operations in future projects of separationprocesses, thereby reducing capital and operating costs. Chitosanases are enzymes that hydrolyze the carbohydratechitosan, resulting in oligosaccharides that have many remarkable biological activities, such as anti-cancer, anti-HIVand antioxidant activities. The two adsorbents had similar performance in relation to hydrodynamics and mass transfer. The results of the parametric sensitivity analysis agree with the literature, and the model was validated with an averagehigh degree of fit (94.68%) between simulated and experimental data obtained in this work.
Everaldo Silvino dos Santos,Carlos Eduardo de Araújo Padilha,Nathália Kelly de Araújo,Domingos Fabiano de Santana Souza,Jackson Araújo de Oliveira,Gorete Ribeiro de Macedo 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.9
A phenomenological model was used to describe sequentially the three steps (flowthrough, washing and elution) of expanded bed adsorption chromatography for recovery of chitosanases from Bacillus cereus. Additionally, a hybrid strategy for model parameter estimation was carried out using particle swarm optimization and Gauss-Newton algorithms. The model was validated with independent experimental data and the statistical criteria (χ 2 and mean squared error tests) showed that the hybrid strategy was more promising than just the heuristic method. With the calibrated model, surface response methodology was applied to obtain the optimal operational conditions, and experiments were performed to confirm these results. Overall, a value of 41.08% for yield was obtained using 700mM NaCl during elution. In summary, all approach employed in this work was relevant for maximizing the yield of the chromatographic process.