조기 후두암의 Docetaxel-Cisplatin 유도 화학요법에 대한 임상연구

정주섭,권보란,최영진,왕수건,이병주,조군제 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

Highly Efficient Agrobacterium Tumefaciens-medicated Transformation of a Forage Legume Medicago Trunctula Using Leaf Explants

Goon-Bo Kim,Seong-Wook Son,Ara Cho,Jeong-Hwan Mun 한국원예학회 2018 한국원예학회 학술발표요지 Vol.2018 No.10

Analysis of Physicochemical Properties and Oil Compounds of the Soil Near Kyuoungiu Railroad site

Bo-Hyun Kim,Eun-Hui Park,Goon-Taek Lee,Kye-Hoon Kim 한국토양비료학회 2005 한국토양비료학회 학술발표회 초록집 Vol.2005 No.9

Roles of medicago truncatula remorin genes in nodule- and arbuscular mycorrhizal symbioses

Goon Bo Kim,Young Woo Nam 한국육종학회 2010 한국육종학회 학술발표회 발표요지 Vol.42 No.1

全國 主要 都市에 錄陰樹로 植栽된 느티나무의 健康度 測定과 이를 利用한 大氣汚染狀態 推定

金君保,韓心熙,李景俊 서울大學校 農業生命科學大學 附屬樹木園 1995 서울大學校 樹木園 硏究報告 Vol.- No.15

本 調査는 大都市 및 工團地域에 綠陰樹로 植栽된 느티나무의 健康狀態를 測定하고, 이를 利用하여 大氣汚染狀態를 推定하기 위하여 實施되었다. 健康 診斷에 利用된 內容은 生長量, 잎의 可視的 被害, 葉綠素 含量, 카로테노이드 含量, 樹木 活力度이다. 1995年 8月 15日부터 21日까지 實施된 全國 調査에서는 3個의 山岳地域을 對照區(淸淨地域)로 하여, 6個의 大都市와 3個의 工團地域을 汚染地로 調査하였고, 서울市 調査에서는 總 19個 地域을 調査하였다. 生長量으로써 新梢 길이, 節間 길이, 잎의 크기 및 갯수, 그리고 잎의 可視的 被害와 樹皮酸度를 測定하였으며, 葉綠素와 카로테노이드 含量은 DMSO(dimethyl sulphoxide)로 抽出 후 分光分析器를 利用하여 測定하였다. 樹木 活力度는 Shigometer를 利用하여 形成層의 電氣 抵抗度로 表示하였다. 新梢生長量, 節間 길이, 잎의 개수의 測定 結果는 地域間 有意性이 認定되었으나 地域間, 個體間 變異가 심하여 일정한 傾向을 볼 수 없었으며, 樹皮 pH는 工團地域에서 가장 낮았다. 對照區를 제외한 全地域에서 可視的 被害가 나타났으며 특히 工團地域에서 심하였다. 葉綠素와 카로테노이드 含量, 樹木活力度는 山岳地域과 比較하여 大都市에서 낮고, 大氣汚染이 심한 工團地域에서 가장 낮았으며 統計的인 差異가 認定되었다. 樹木活力度는 葉綠素나 카로테노이드 含量과 높은 相關 關係를 보였으며, 특히 서울 地域에서 높은 相關係數(葉綠素 : r²=0.58, 카로테노이드 : r²=0.55)를 보였다. 健康度는 可視的 被害, 葉綠素와 카로테노이드 含量과 電氣 抵抗度의 네 가지 項目을 사용할 때 가장 信憑性 있게 測定할 수 있었다. 서울市 調査에서는 江南地域이 江北보다 被害 症狀이 컸으며, 특히 大氣汚染이 심한 구로 工團과 永登浦驛을 포함한 江南 西部 地域이 가장 심한 症狀을 보였다. 全國 느티나무의 健康度는 大氣汚染이 심할수록 낮게 나타났으며, 느티나무는 大氣汚染을 間接的으로 測定하는 指標植物로 活用할 수 있다고 結論 짓는다. This study was conducted to measure shade tree health in major cities and to test the possibility of using the health data to indirectly estimate status of air pollution in the same areas. Zelkova serrata Makino planted as a shade tree in six largest cities and three industrial complexes was measured in mid August for growth increment, visible leaf damage, bark pH, chlorophyll and β-carotene contents, and tree vitality. The results were compared with those of healthy trees in three mountain areas as a control. Cholorophyll and β-carotene were extracted in dimethyl sulphoxide solution and quantified by spectrophotometry, while tree vitality was estimated by measuring electrical resistance in the cambium using Shigometer. Growth parameters such as new shoot length, number of leaves, or width of annual rings varied widely in various locations. Visible leaf damage was observed in most of the sites except mountain areas, with heaviest damage in industrial complexes. Chlorophyll and β-carotene contents, bark pH, and tree vitality were lower in six major cities and lowest in industrial complexes, compared to those in mountain areas. Tree vitality was positively correlated with chlorophyll(r²=0.58) and β-carotene content(r²=0.55), and negatively correlated with degree of leaf damage. Tree health can be estimated most successfully by combining data on visible leaf damage, chlorophyll and β-carotene contents and electrical resistance. Among the 19 sites surveyed in Seoul metropolitan areas, west side of Kangnam(south of River Han) where Kuro and Yongdungpo industrial complexes were located showed lowest tree health and Kangnam showed lower tree health than Kangpuk(north of River Han). It is concluded that Zelkova serrata trees are sensitive to air pollution and can be used as a bioindicator to indirectly monitor air pollution status in large cities and industrial complexes.

Upregulation of the serine palmitoyltransferase subunit SPTLC2 by endoplasmic reticulum stress inhibits the hepatic insulin response

Kim Goon-Tae,Devi Shivani,Sharma Amitesh,Cho Kyung-Hee,Kim Su-Jung,Kim Bo-Rahm,Kwon Sang-Ho,Park Tae-Sik 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Endoplasmic reticulum (ER) stress is induced by various conditions, such as inflammation and the presence of excess nutrients. Abnormal accumulation of unfolded proteins leads to the activation of a collective signaling cascade, termed the unfolded protein response (UPR). ER stress is reported to perturb hepatic insulin response metabolism while promoting insulin resistance. Here, we report that ER stress regulates the de novo biosynthesis of sphingolipids via the activation of serine palmitoyltransferase (SPT), a rate-limiting enzyme involved in the de novo biosynthesis of ceramides. We found that the expression levels of Sptlc1 and Sptlc2, the major SPT subunits, were upregulated and that the cellular concentrations of ceramide and dihydroceramide were elevated by acute ER stress inducers in primary hepatocytes and HepG2 cells. Sptlc2 was upregulated and ceramide levels were elevated by tunicamycin in the livers of C57BL/6J wild-type mice. Analysis of the Sptlc2 promoter demonstrated that the transcriptional activation of Sptlc2 was mediated by the spliced form of X-box binding protein 1 (sXBP1). Liver-specific Sptlc2 transgenic mice exhibited increased ceramide levels in the liver and elevated fasting glucose levels. The insulin response was reduced by the inhibition of the phosphorylation of insulin receptor β (IRβ). Collectively, these results demonstrate that ER stress induces activation of the de novo biosynthesis of ceramide and contributes to the progression of hepatic insulin resistance via the reduced phosphorylation of IRβ in hepatocytes.

후천성 면역 결핍증 환장서 발생한 뇌톡소플라즈마증 1례

김보현,이승익,이창훈,차승헌,이태홍,이선희,정주섭,조군제 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

뇌톡소플라즈마증은 후천성면역결핍증 환자에서 발생하는 중추신경계 기회감염증의 가장 흔한 원인 중의 하나이다. 그 빈도는 지역마다 다양하지만 우리나라에서는 매우 드물게 보고되고 있다. 지금까지 우리나라에서 보고된 후천성면역결핍증 환자에서 발생한 뇌톡소플라즈마증 증례들은 모두 혈청학적으로 진단한 것으로 조직학적으로 증명된 경우는 없었다. 저자들은 HIV 감염 후 약 10년 동안 특별한 치료를 받지 않고 지내다 뇌의 다발성 종괴로 내원하여 조직학적으로 확진된 뇌톡소플라즈마증 1례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Toxoplasrnosis is one of the most common opportunistic infection of the central nervous system in patients with acquired immunodeficiency syndrome(AIDS). There have been few reports of cerebral toxoplasmosis in patients with AIDS in Korea. In most cases, the diagnosis was assisted by serology and neuroradiologic findings. Making a reliable diagnosis of acute cerebral toxoplasmosis is difficult in patients with AIDS because of the lack of specificity of serologic data and neuroradiological findings. We report a case of 32-year-old man who presented with decreased mentality and fever. Brain MRI showed multiple ill-defined mass-like lesions in both basal ganglia and right thalamus. Stereotatic brain biopsy revealed small parasitic cysts which were filled with toxoplasmic bradyzoites in inflammatory brain tissue.

Predicting family-wide consensus sequence of pre-mRNA splicing signals in legumes and their potential utility as phylogenetic markers

최홍규,이채영,Goon-Bo Kim,남영우,Kenneth R. Schraufnagle,정영수,이재헌,김도훈,정호원 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.2

Recent studies have shown that an intron is notmerely ‘‘junk’’, but something that plays important roles inmany biological processes such as gene expression regulationand alternative splicing. For purposes of studyingintron structures and predicting consensus splice motifs, atotal of 102 legume species were used to isolate intronsacross the family. Of 196 gene-targeted PCR primer pairs,we successfully amplified 118 intron-containing genes(60.2 %) and obtained a total of 1,870 introns with anaverage size of 143 nucleotides, ranging from 61 to 1,036. Species-based compilation of 50- and 30-splicing motifsshowed, to some extent, lineage-specific conservation ineach splicing motif. Compilation of the entire intron setpermitted prediction of the consensus sequences of splicingsignal motifs in legumes, AYGWGTABABGH and TVNC/TAGGHTV for the 50SS and 30SS, respectively. Interestingly,these consensus motifs are very similar to the correspondinggenome-wide splicing signals of two modelsystems, Arabidopsis and rice. This result suggests conservationof pre-mRNA splicing mechanism occurring inhigher plants. Multiple alignments of CALTL intronsdemonstrated that the BP-30SS region was relatively moreconserved than the 50SS-BP region. We speculate thatlength of the BP-30SS region needs to be retained for theinteraction with U2AF protein. Phylogenetic analysisdemonstrates that each of three splicing motifs is not onlyphylogenetically informative, but also relevant to evolutionarydivergence of species. This result suggests that thesplice signal sequences would be a useful tool for themolecular phylogenetic analysis. We also anticipate thatgene-targeted amplification in multiple genomes, describedin this study, would facilitate studies on intron-locatedfunctional elements involved in gene expressionregulations.

재활용을 위한 플라스틱 부산물의 열분해 특성

김규보(Gyu-Bo Kim),김량균(Haak-Rho Lee),임호(Ryang-Goon Kim),박영도(Ho Lim),이학로(Young-Do Park) 대한기계학회 2023 대한기계학회 춘추학술대회 Vol.2023 No.6

지리산 구상나무림에서 타감작용이 치수형성에 미치는 영향

김군보,이경준,현정오 ( Goon Bo Kim,Kyung Joon Lee,Jung Oh Hyun ) 한국산림과학회 1998 한국산림과학회지 Vol.87 No.2

This study was conducted to compare the regeneration of the seedlings under different vegetation types and to identify the presence of allelopathy in Abies koreana(Ak) natural forest in Banyabong Peak(elevation, 1715m), of Mt. Chiri. Twenty quadrats(10m×10m) were placed in May. 1996 to classify vegetation structure using TWINSPAN. Water-soluble extracts from leaves and soil humus of different vegetation types were collected to test their effects on both seed germination of Ak and mycelial growth of ectomycorrhizal fungi. Phenolic compounds from soil humus were quantified using HPLC. Among the four vegetation types, Sasa borealis(Sb) was found in both Ak-Quercus mongolica(Qm) and Ak-Rhododendron schlippenbachii(Rs) communities. Natural seeding of Ak was 230,000±90,000 seeds/㏊ in 1995 and their germination rate was 25% in an ideal laboratory condition. Density of Ak seedlings less than 5㎝ in height was 52,000/㏊ in 1996, while that of seedlings taller than 5㎝ in height was only 4,000/㏊. In the case of Ak-Qm community, density of Ak seedlings with Sb understory was only 7% of the density of seedlings with Rs understary, suggesting the inhibitory effect of Sb. The germination rate of Ak seeds was significantly reduced by leaf extracts of Sb, and Rhododendron mucronulatum var. ciliatum(Rm) and Ak. Soil humus extract of Ak-Qm-Sb subcommunity reduced germination of Ak seeds by 81% and also reduced by 19% the respiration of mycelia of ectomycorrhizal fumgus, Lactarius sp. Among the seven phenolic compounds identified from the soil humus, extract, Ak-Qm-Sb subcommunity contained significantly high content of p-hydroxybenzoic acid, vanillic acid, and syringic acid. Particularly, p-hydroxybenzoic acid was present at 4.2ppm in the Sb roots and at 16.5ppm in the Sb humus, suggesting that it could be the primary allelopathic compound in Abies koreana forests with Sasa borealis understory.