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Gonzalez-Cabrera Jaime,Moreno-Carrillo Gabriel,Lopez-Juarez Alejandro A.,Anguiano-Amezcua Juan A.,Sanchez-Gonzalez Jorge A.,Mendoza-Ceballos Mario Y.,Arredondo-Bernal Hugo C. 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Two colonies of the parasitoid Trichopria drosophilae (Perkins) (Hymenoptera: Diapriidae) are reproduced using a mass rearing approach in Mexico. The first colony uses the worldwide commercial host Drosophila melanogaster Meigen (Diptera: Drosophilidae) reared on an artificial diet (=T. drosophilae-Dm), while the second colony uses D. suzukii Matsumura reared on fresh banana (=T. drosophilae-Ds). Since theoretically the type of colony host affects parasitoid field performance, we conducted concatenated laboratory and field studies of both parasitoid lines. In the laboratory trials, T. drosophilae-Ds exhibited greater longevity, larger adult size, and higher fecundity than T. drosophilae-Dm. Both lines of parasitoids oviposited indistinctly in three drosophilid species commonly found in the berry zones of Colima: D. melanogaster, D. suzukii, and Zaprionus indianus Gupta. In the field trials, T. drosophilae-Ds had a larger population size and provided 2.4-fold higher pest reduction compared to T. drosophilae-Dm. Under the particular conditions of these field trials (berry fields separate from each other ≤ 3 km, and intensive agronomic management), these results demonstrate that T. drosophilae-Ds can significantly contribute more to the integrated management of D. suzukii than when reared on the commercial host D. melanogaster.
Gonzalez-Cantu, Cynthia Minerva,Moreno-Pena, Pablo Juan,Salazar-Lara, Mayela Guadalupe,Garcia, Pablo Patricio Flores,Montes-Tapia, Fernando Felix,Cervantes-Kardasch, Victor Hugo,Castro-Govea, Yanko Korean Society of Plastic and Reconstructive Surge 2021 Archives of Plastic Surgery Vol.48 No.5
Epignathus is a rare congenital orofacial teratoma that arises from the sphenoid region of the palate or the pharynx. It occurs in approximately 1:35,000 to 1:200,000 live births representing 2% to 9% of all teratomas. We present the case of a newborn of 39.4 weeks of gestation with a tumor that occupied the entire oral cavity. The patient was delivered by cesarean section. Oral resection was managed by pediatric surgery. Plastic surgery used virtual 3-dimensional models to establish the extension, and depth of the tumor. Bloc resection and reconstruction of the epignathus were performed. The mass was diagnosed as a mature teratoma associated with cleft lip and palate, nasoethmoidal meningocele that conditions hypertelorism, and a pseudomacrostoma. Tridimensional technology was applied to plan the surgical intervention. It contributed to a better understanding of the relationships between the tumor and the adjacent structures. This optimized the surgical approach and outcome.
Gonzalez, Santiago R.,Hobbs, Bradley,Vural, Emre,Moreno, Mauricio A. Korean Association of Maxillofacial Plastic and Re 2019 Maxillofacial Plastic Reconstructive Surgery Vol.41 No.-
Background: Advancements in the field of microvascular surgery and the widespread adoption of microvascular surgical techniques have made the use of osteocutaneous fibula free flaps the standard of care in the surgical management of segmental mandibular defects. Although the literature possesses abundant evidence to support the effectiveness of fibula free flaps as a reconstructive method, there are relatively few studies reporting on outcomes as objectively measured by videofluoroscopic swallowing studies (VFSS). The purpose of this study is to explore the potential correlation between early postoperative VFSS and the long-term swallowing outcomes in patients who underwent mandibular reconstruction with fibula free flaps. Methods: We performed a retrospective chart review of 36 patients who underwent mandibular reconstruction with osteocutaneous fibular free flaps between 2009 and 2012. Demographics, clinical variables, VFSS data, and diet information were retrieved. Penetration and aspiration findings on VFSS, long-term oral feeding ability, and the need for gastrostomy tube were statistical endpoints correlated with postoperative clinical outcomes. Results: Thirty-six patients were reviewed (15 females and 21 males) with a mean age of 54 years (7-81). Seventeen cases were treated for malignancy. The size of the bony defect ranged from 3 to 15 cm (mean = 9 cm). The cutaneous paddle, a surrogate for soft tissue defect, ranged from 10 to 125 ㎠ (mean = 52 ㎠). A gastrostomy tube was present in patients preoperatively (n = 8), and postoperatively (n = 14). Seventeen patients had neoadjuvant exposure to radiation. Postoperative VFSS showed penetration in 13 cases (36%) and aspiration in seven (19%). Overall, 29 patients (80.6%) achieved unrestricted diet, and this was statistically correlated with age (p = 0.037), radiation therapy (p = 0.002), and preoperative gastrostomy tube (p = 0.03). The presence of penetration or aspiration on VFSS was a strong predictor for long-term unrestricted oral diet (p < 0.001). Conclusion: Early postoperative VFSS is an excellent predictor for long-term swallowing outcomes in patients undergoing mandibular reconstruction with osteocutaneous fibula free flaps.
( Moreno Enriquez Angelica ),( Zahaed Evangelista Martinez ),( Edith G. Gonzalez-mondragon ),( Arturo Calderon Flores ),( Roberto Arreguin ),( Ernesto Perez Rueda ),( Alejandro Huerta Saquero ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3
We report the expression, purification, and characterization of L-asparaginase (AnsA) from Rhizobium etli. The enzyme was purified to homogeneity in a single-step procedure involving affinity chromatography, and the kinetic parameters Km, Vmax, and kcat for L-asparagine were determined. The enzymatic activity in the presence of a number of substrates and metal ions was investigated. The molecular mass of the enzyme was 47 kDa by SDS-PAGE. The enzyme showed a maximal activity at 50oC, but the optimal temperature of activity was 37oC. It also showed maximal and optimal activities at pH 9.0. The values of Km, Vmax, kcat, and kcat/Km were 8.9±0.967×10-3 M, 128±2.8 U/mg protein, 106±2 s-1, and 1.2±0.105×104M-1s-1, respectively. The L-asparaginase activity was reduced in the presence of Mn2+, Zn2+, Ca2+, and Mg2+ metal ions for about 52% to 31%. In addition, we found that NH4 +, L-Asp, D-Asn, and β-aspartyl-hydroxamate in the reaction buffer reduced the activity of the enzyme, whereas L-Gln did not modify its enzymatic activity. This is the first report on the expression and characterization of the L-asparaginase (AnsA) from R. etli. Phylogenetic analysis of asparaginases reveals an increasing group of known sequences of the Rhizobialtype asparaginase II family.
Gonzalez-Herrera, A.L.,Salgado-Bernabe, M.,Velazquez-Velazquez, C.K.,Salcedo-Vargas, M.,Andrade-Manzano, A.,Avila-Moreno, F.,Pina-Sanchez, P. Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4
Background: Cervical cancer (CeCa) is the second most common cancer in women in developing countries, and human papilloma virus (HPV) is the primary etiological factor. Aberrant expression of HOX transcription factors has been observed in several types of cancer. To date, however, no reports exist on the expression of HOXB2 and HOXB13 proteins during neoplastic progression in CeCa and its correlation with HPV infection. Materials and Methods: Expression of HOXB2 and HOXB13 proteins was assessed in tissue microarrays from normal cervical epithelium, cervical intraepithelial neoplasias grade 1-3, and CeCa. HPV was detected by PCR and sequencing. Expression of HOX-positive cells was determined in each diagnostic group. Results: Percentage of HOXB2- and HOXB13-positive cells gradually increased from means of 10.9% and 16.7%, respectively, in samples from healthy women, to 75.2% and 88.6% in those from CeCa patients. Frequency of HPV infection also increased from 13% in healthy tissue samples to 92.3% in CeCa. Both HOXB2 and HOXB13 proteins were preferentially expressed in HPV+ samples. Conclusions: The present study represents the first report on the expression of both HOXB2 and HOXB13 proteins through cervix tumorigenesis, providing evidence that increased expression of such proteins is a common event during progression to CeCa.
( Angelica Moreno Enriquez ),( Zahaed Evangelista Martinez ),( Luis Servin Gonzalez ),( Maria Elena Flores Carrasco ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.8
The aim of the present work was to examine the putative promoter region of the operon ansPAB and to determine the general elements required for the regulation of transcriptional activity. The transcriptional start site of the ansPAB promoter was determined by using highresolution S1-nuclease mapping. Sequence analysis of this region showed -10 and -35 elements, which were consistent with consensus sequences for R. etli promoters that are recognized by the major form of RNA polymerase containing the σ70 transcription factor. Mutation studies affecting several regions located upstream of the transcriptional start site confirmed the importance of these elements on transcriptional expression.
( Peter Buggisch ),( Ana Moreno ),( Vasily Isakov ),( Lisa Backus ),( Dani Ain ),( Peter Ruane ),( Juan Gonzalez ),( Sooji Lee ),( Sarjita Naik ),( Swarup Mehta ),( Jina Lee ),( Michael Mertens ),( Ma 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: The AASLD/IDSA/IAS-USA Guidance and EASL Recommendations on Treatment of Hepatitis C state that HIV/HCV coinfection should be treated the same as HCV monoinfection with careful monitoring of antiretrovirals. Real world cohorts (RWC) have demonstrated excellent efficacy of LDV/SOF for 8weeks in HCV monoinfected patients. The aim of this study was to describe the effectiveness of the STR of LDV/SOF for 8 weeks in HCV GT 1 patients with HIV/HCV coinfection in RWC. Methods: Real world effectiveness data of LDV/SOF for 8weeks in HIV/HCV coinfection is emerging from multiple cohorts. In this descriptive analysis, data from two prospective studies, one investigator- sponsored, 1 registrational trial, and three retrospective RWC of LDV/SOF for 8weeks in HIV/HCV co-infected patients were compared. The prospective trials include data from Ain et al (investigator sponsored) and Isakov et al (registrational trial). The RWC include the Deutsches Hepatitis C-Register, Madrid Coinfection Registry (Madrid-CoRe), and Veterans Affairs HCV Registry. Baseline characteristics and efficacy were analyzed. Results: The majority of the 279 patients included in this descriptive analysis were GT1, treatment naive (TN), noncirrhotic (NC), and had a HCV viral load <6million. The prospective cohorts enrolled 79 patients with the following baseline characteristics: mean age (43 years), male (74%), white (78%), and GT1a (55%). The RWC studies assessed enrolled 200 patients with the following overall baseline characteristics: mean age (53 years) male (79%), white (98%), and GT1a (82%) in those that reported demographics. The overall SVR12 from five diverse real world and post-marketing cohorts was 94% (263/279). The individual study results are presented in Table 1. Conclusions: This analysis of diverse cohorts from the EU and US yielded high SVR rates similar to SVR rates seen in multiple RW moninfected cohorts and supports the use of 8 weeks of LDV/SOF in TN, NC GT 1 HIV/HCV coinfected patients with a baseline HCV viral load <6 million.