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Eukaryotic cilia are organelles that project from the surface of cells to fulfill motility and sensory functions. In vertebrates, the functions of both motile and immotile cilia are critical for em-bryonic development and adult tissue homeostasis. Importantly, a multitude of human diseases is caused by abnormal cilia biogenesis and functions which rely on the com-partmentalization of the cilium and the maintenance of its protein composition. The transition zone (TZ) is a specialized ciliary domain present at the base of the cilium and is part of a gate that controls protein entry and exit from this organelle. The relevance of the TZ is highlighted by the fact that several of its components are coded by ciliopathy genes. Here we review recent developments in the study of TZ proteomes, the mapping of individual compo-nents to the TZ structure and the establishment of the TZ as a lipid gate.
Isadora,Gonçalves,da,Silva,Renata,Castoldi,Hamilton,César,de,Oliveira,Charlo,Mateus,de,Souza,Miranda,Thaíssa,Dias,Cardoso,Nunes,Luciene,Lacerda,Costa,Ernane,Miranda,Lemes 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.2
The cultivation of sweet corn is expanding in Brazil, but there are serious constraints about the availability of commercial cultivars. The selection of superior sweet corn genotypes can be performed based on selection indexes based on plant agronomic characteristics. Thus, the objective of this work was to compare selection indexes to select sweet corn genotypes aiming greater productions. The experiment was conducted at the Vegetable Experimental Station of the Federal University of Uberlândia, Campus Monte Carmelo. Eighteen sweet corn accessions (F3 generation) were evaluated. The selection indexes applied to the agronomic characteristics of sweet corn were: direct and indirect, the sum of ranks, desired gains and ideotype indexes. The characteristics evaluated presented significant differences among genotypes, except for stem diameter, prolificacy and grain number per corncob. The coefficients of variation were below 30%, and the genetic parameters were satisfactory for most of the characteristics. The greatest gains with direct selection were in production (21.23%) and productivity of commercial sweet corn ear (15.19%), however, the indirect gains are unsatisfactory for sweet corn selection, and the sum of ranks index provided a balanced distribution of gains. The sweet corn genotypes L2P11, L2P37, P45, L2L5P3, and L5P18 presented a superior performance for the set of characters evaluated.
This paper first proposes a brief comparison between the phonological systems of Korean and Brazilian Portuguese, using the Model of Contrastive Analysis. Such comparison is then used at predicting and testing pronunciation mistakes made by Korean speakers who learn Brazilian Portuguese, in order to verify which aspects need more attention by both teacher and student. Finally, it presents an analysis of six Portuguese language books for Korean learners and checks how they approach and/or explain pronunciation to the readers. The findings reveal that teaching pronunciation in Korea is still a field little explored and yet to be developed, especially in specific contexts such as teaching Brazilian Portuguese pronunciation for Korean speakers.
We developed and implemented an intelligent control system to be used in an extractive distillation column that produces anhydrous ethanol using ethylene glycol as solvent. The concept of artificial neural networks (ANN) was used to predict new setpoints after disturbances, and proved to be a fast and feasible solution. The developed control system receives data from temperature, flowrate and composition measurements of the azeotrope feed, and the ANN estimates the new set-points of the controllers to maintain 99.5mol% of ethanol at the top and less than 0.1mol% at the bottom; feed composition was also estimated using an ANN. All ANN were trained to provide output data corresponding to an optimized operating condition. The results showed that the intelligent control system can predict a new operating condition for any disturbance in the column feed and presented superior performance when compared with the control system without ANN.
Priscila,Gonçalves,Ferreira,Fernando,Augusto,da,Silveira,Raquel,Cristina,Vieira,dos,Santos,Hugo,Leonardo,André,Genier,Raphael,Hermano,Santos,Diniz,José,Ivo,Ribeiro,Júnior,Luciano,Gomes,Fietto,Flávia,M 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
The simultaneous saccharification and fermentation (SSF) process is a promising strategy to obtain ethanol from cellulosic biomass. In this study, sugarcane bagasse was supplemented with ricotta whey to increase the sugar, vitamin, and trace metal concentrations in the fermentation medium. The optimum conditions for SSF ethanol production from a mixture of sugarcane bagasse and ricotta whey produced by Kluyveromyces marxianus CCT 7735 were evaluated considering five factors: cellulase concentration, cellulosic biomass concentration, pH, temperature, and agitation. The highest ethanol yield was 49.65 g/L with a cellulosic biomass of 80 g/L, pH value of 5.05, agitation at 65 rpm and temperature of 39.2ºC. The results demonstrated that a mixture of the cellulosic residue of sugarcane bagasse and ricotta whey is promising for ethanol production because the ethanol yield in the mixture was higher than that in single substrate of sugarcane bagasse.
본 연구는 2012년 동티모르 총선 과정에서 보여진 동티모르 여성의 참여를 정치적, 행정적 그리고 유권자의 관점에서 살펴보고 있다. 기존 동티모르에 대한 연구들은 주로 지역 수준에서의 여성할당제, 시민사회의 젊은 여성, 그리고 양성평등 촉진을 목적으로한 공여자들의 역할에 대해 다루었다. 그러나 2006년 여성할당제 실시에도 불구하고, 2012년 선거과정은 선거에 대한 인식과 참여라는 점에서 남녀의 차이를 분명히 보여주는 계기가 되었다. 대부분의 관점에서 볼 때, 여전히 동티모르 남성들은 선거의 유일한 행위자이며 양성평등 촉진을 위해서는 새로운 접근이 시도될 필요가 있다. This paper examines the participation of Timorese women in the electoral process during the 2012 national elections, from the political, administrative and voters' point of view. Existing literature on Timor-Leste has explored the issue of gender quotas at the local level, young women in civil society, and the role of aid donors at promoting gender equality in the country. However, the 2012 electoral process was a good opportunity to observe the gap between men and women in terms of electoral participation and consciousness in spite of the advances obtained through the gender quotas implemented in 2006. Findings confirm that Timorese men are still the sole conductors of the elections from most perspectives, and a new approach is necessary in order for the country to advance further in gender equality.
Reinforced concrete structures are widely used in civil engineering projects around the world in different designs. Due to the great evolution in computational equipment and numerical methods, structural analysis has become more and more reliable, and in turn more closely approximates reality. Thus among the many numerical methods used to carry out these types of analyses, the finite element method has been highlighted as an optimized tool option, combined with the non-linear and linear analysis techniques of structures. In this paper, the behavior of reinforced concrete beams was analyzed in two different configurations: i) with welding and ii) conventionally lashed stirrups using annealed wire. The structures were subjected to normal and tangential forces up to the limit of their bending resistance capacities to observe the cracking process and growth of the concrete structure. This study was undertaken to evaluate the effectiveness of welded wire fabric as shear reinforcement in concrete prismatic beams under static loading conditions. Experimental analysis was carried out in order compare the maximum load of both configurations, the experimental load-time profile applied in the first configuration was used to reproduce the same loading conditions in the numerical simulations. Thus, comparisons between the numerical and experimental results of the welded frame beam show that the proposed model can estimate the concrete strength and failure behavior accurately.
The extracellular β-D-fructofuranosidase from Fusarium graminearum was immobilized using hydrophilic cotton, filter paper, multipurpose cloth, sugar cane bagasse, string, or gauze as alternative cellulosic supports, or with cyanogen bromide agarose. All derivatives (support+enzyme) showed high capacity for reuse (up to 23 times). The derivatives obtained with multipurpose cloth and string were stable at 60℃ maintaining 80% of their activity for more than 120 min. The filter paper derivative had a halflife (T50) of 27 min at 70℃. When tested for their pH stability (3.0-9.0), all derivatives were more stable than the free enzyme, especially the cotton derivative. The sugarcane bagasse, string, and filter paper derivatives could efficiently produce fructooligosaccharides (FOS) using sucrose as substrate. According to the retention of enzymatic activity, reuse and stabilities, the filter paper and string were the best alternative supports for β-D-fructofuranosidase immobilization, allowing enzyme stabilization and production of FOS.