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Cryopreservation and low-temperature storage of seeds of Phaius tankervilleae
Tomonari Hirano,Toshinari Godo,Kazumitsu Miyoshi,Keiko Ishikawa,Masaya Ishikawa,Masahiro Mii 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.1
In this study we established reliable methods for conservation of seeds of Phaius tankervilleae as an orchid genetic resource. The seeds, which were dehydrated to 5% water content and preserved at 4C, showed no decrease in viability and germinability after three months. After storage for six months, however, the seeds showed a drastic decrease in germinability, even though survival rate was high. For long-term preservation of seeds of P. tankervilleae, cryopreservation is applied to the freshly harvested seeds. When the seeds were cryopreserved by the vitrification method for up to 12 months there was no apparent deterioration effect of storage time. These results indicate that cryopreservation by the vitrification method is useful for long-term conservation of P. tankervilleae seeds, which are difficult to preserve for more than three months under dry and low-temperature conditions. In this study we established reliable methods for conservation of seeds of Phaius tankervilleae as an orchid genetic resource. The seeds, which were dehydrated to 5% water content and preserved at 4C, showed no decrease in viability and germinability after three months. After storage for six months, however, the seeds showed a drastic decrease in germinability, even though survival rate was high. For long-term preservation of seeds of P. tankervilleae, cryopreservation is applied to the freshly harvested seeds. When the seeds were cryopreserved by the vitrification method for up to 12 months there was no apparent deterioration effect of storage time. These results indicate that cryopreservation by the vitrification method is useful for long-term conservation of P. tankervilleae seeds, which are difficult to preserve for more than three months under dry and low-temperature conditions.
윤동원(Dongwon Yun),윤소남(Sonam Yun),박중호(Jungho Park),김고도(Godo Kim),조정현(Junghyun Jo) 한국자동차공학회 2007 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
In the field of diesel vehicle, it is important to make fuel atomized and to generate high pressure to reduce the amount of toxin gas and increase the efficieny. So high pressure control valve is widely used in diesel engine and a solenoid valve is of ten used in that kind of valve. In this paper, transient response of solenoid is investigated using Maxwell, commercial FEA code for electromechanical system. Firstly, an experiment about electrical response is performed and then some FE analysis is performed using Maxwell. After that, two results are compared to validate the result of the FEA. Secondly, the system is analyzed considering mechanical parts and the electro-mechanical response is solved.
Joerg D Moritz,Beata Hoffmann,Dirk Sehr,Katrin Keil,Juliane Eggerking,Godo Groth,Amke Caliebe,Jens Dischinger,Martin Heller,Hendrik Bolte 대한영상의학회 2012 Korean Journal of Radiology Vol.13 No.2
Objective: The aim of this prospective study was to evaluate the performance of ultra-low dose CT for the diagnosis of pediatric-like fractures and ascertain the lowest dose level sufficient for diagnostics. Materials and Methods: Fifty-one bones of young pig cadavers were artificially fractured and subsequently examined by using a 64 multi-detector CT with 36 various dose levels down to a dose comparable with that of X-rays. Two pediatric radiologists analysed the CT scans according to the presence or absence of a fracture, determination of the fracture type and the displacement as well as the diagnostic certainty. For each dose protocol, a success rate for the correct determination of the above-mentioned CT analyses was calculated. A success rate of at least 95% was considered sufficient for diagnostics. Results: All but the lowest dose levels were sufficient to identify the fracture. Only the two lowest dose levels were insufficient to detect the fracture type. All dose levels were adequate for the identification of the displacement. The lowest dose level sufficient for diagnostics was 120 kVp, 11 mAs, and pitch 1.5, with a CTDIvol of 10% of a standard dose and an effective dose three times as large as that of X-rays. Conclusion: Ultra-low dose CT provides the feasibility of a significant dose reduction, still allowing sufficient diagnostics of pediatric-like fractures. Objective: The aim of this prospective study was to evaluate the performance of ultra-low dose CT for the diagnosis of pediatric-like fractures and ascertain the lowest dose level sufficient for diagnostics. Materials and Methods: Fifty-one bones of young pig cadavers were artificially fractured and subsequently examined by using a 64 multi-detector CT with 36 various dose levels down to a dose comparable with that of X-rays. Two pediatric radiologists analysed the CT scans according to the presence or absence of a fracture, determination of the fracture type and the displacement as well as the diagnostic certainty. For each dose protocol, a success rate for the correct determination of the above-mentioned CT analyses was calculated. A success rate of at least 95% was considered sufficient for diagnostics. Results: All but the lowest dose levels were sufficient to identify the fracture. Only the two lowest dose levels were insufficient to detect the fracture type. All dose levels were adequate for the identification of the displacement. The lowest dose level sufficient for diagnostics was 120 kVp, 11 mAs, and pitch 1.5, with a CTDIvol of 10% of a standard dose and an effective dose three times as large as that of X-rays. Conclusion: Ultra-low dose CT provides the feasibility of a significant dose reduction, still allowing sufficient diagnostics of pediatric-like fractures.
Optical characterization of CdTe/ZnSe fractional monolayer structures grown by atomic layer epitaxy
J.H.Chang,J.Y.Lee,M.Yang,H.S.Ahn,S.N.Yi,K.Goto,K.Godo,H.Makino,M.W.Cho,T.Yao,J.S.Song 한국물리학회 2004 Current Applied Physics Vol.4 No.6
Luminescence properties of CdTe/ZnSe fractional monolayer grown by atomic layer epitaxy have been investigated. To inves-tigate the origin of the highly ecient luminescence, various optical spectroscopic methods such as, photoluminescence (PL),temporal/spatial resolved PL, temperature dependence PL, and excitation power dependence PL have been used. It is found thatstructural inhomogeneities aect dominant inuence on the line width and line shape of luminescence. The luminescence intensitygreatly enhanced by the localization of exciton at the disorder induced localized states.
Takagi, Hiroki,Sugawara, Shintaro,Saito, Tomoka,Tasaki, Haruka,Yuanxue, Lu,Kaiyun, Guan,Han, Dong-Sheng,Godo, Toshinari,Nakano, Masaru The Korean Society of Plant Biotechnology 2011 Plant biotechnology reports Vol.5 No.2
The gesneriaceous perennial plant Titanotrichum oldhamii has beautiful foliage and attractive bright yellow flowers. However, breeding of T. oldhamii by conventional sexual hybridization may be difficult because sexual reproduction of this species is very rare. In the present study, plant regeneration systems via both direct and indirect formation of adventitious shoots from leaf explants were established as the first step toward breeding T. oldhamii by using biotechnological techniques. Adventitious shoots were formed efficiently on medium containing $0.1mg\;l^{-1}$ benzyladenine. Histological observation showed that shoot formation on this medium occurred directly from leaf epidermal cells without callus formation. On the other hand, leaf explants formed calluses on medium containing $0.1mg\;l^{-1}$ 2,4-dichlorophenoxyacetic acid. The calluses could be maintained by monthly subculturing to fresh medium of the same composition. When the calluses were transferred to plant growth regulator-free medium, they formed adventitious shoots. Directly and indirectly formed shoots rooted well on medium containing $0.1mg\;l^{-1}$ indole-3-butyric acid. Plantlets thus obtained were successfully acclimatized and grew vigorously in the greenhouse. Flow cytometry analysis indicated that no variation in the ploidy level was observed in plants regenerated via direct shoot formation, whereas chromosome doubling occurred in several plants regenerated via indirect shoot formation. Regenerated plants with the same ploidy level as the mother plants showed almost the same phenotype as the mother plants, whereas chromosome-doubled plants showed apparent morphological alterations: they had small and crispate flowers, and round and deep green leaves.
Leyla Camila Aquije Eyzaguirre,Arturo Daniel Hernández Aparcana,David Rene Rodriguez Diaz,Jully Pahola Calderón Saldaña,Luis Alex Alzamora De Los Godos Urcia 소화기인터벤션의학회 2023 International journal of gastrointestinal interven Vol.12 No.1
Although it has been demonstrated that cholecystectomy is successful in relieving symptoms in 90% of cases, some patients continue to experience gastrointestinal symptoms despite surgical intervention; this is known as postcholecystectomy syndrome. For this reason, we decided to investigate this syndrome and, relying on the article by Desai et al entitled "Foreign bodies in common bile duct in postcholecystectomy state—series of 8 cases: an experience of a single center in western India," delve into the different symptoms that appear in the syndrome. Based on an extensive review of articles published about the syndrome, the most common symptoms are dyspepsia, pain in the upper right quadrant of the abdomen, abdominal distension, and gastrointestinal issues. Consequently, it is critical to take these symptoms into consideration because, even though it may not seem like postcholecystectomy syndrome occurs frequently, one needs to be familiar with its clinical picture.