In vitro cytotoxic evaluation of some essential oils

P., Vijayan,Godavarthi, Ashok,Chandrashekhar, Raghu,Badami, Shrishilappa,SA, Dhanaraj,B., Suresh Kyung Hee Oriental Medicine Research Center 2003 Oriental pharmacy and experimental medicine Vol.3 No.4

Seven essential oils were tested for in vitro cytotoxicity against the cancerous cell lines A-549, HEp-2 and DLA and normal BRL-3A, NRK-49F and Vero cell lines using standard MTT, SRB and dye exclusion techniques. The A-549 cell line was found to be the most susceptible to all the essential oils. The essential oils of A. nilagirica, A. calamus and O. sanctum were found to be the more active against these cells with mean $CTC_{50}$ values of 17.75, 19.00 and $24.37\;{\mu}g/ml$, respectively. The essential oil of Acorus calamus was found to be the most potent with low $CTC_{50}$ values against the cancerous and comparatively higher $CTC_{50}$ values against the normal cell lines. Artemisia pellens and Pelargonium graveolens oils also showed potent activity. These oils merit further investigation to identify the active principles and nature of the anti tumor activity in animal models.

Cytotoxic and antioxidant properties of four plants belonging to the genus Solanum

Santoshkumar H Dongre,Shrishailappa Badami,Ashok Godavarthi, Mahendran S,Vijayan P andSuresh B 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.1

The aim of the study was to evaluate in vitro antioxidant and cytotoxic activities of the methanolic extracts of leaves of Solanum sisymbrifolium, Solanum anguivi multiflora, Solanum barbisetum and Solanum jasminoides. In the in vitro antioxidant screening using ABTS [2,2’-azino-bis (3-ethylbenzo-thiazoline-6- sulphonic acid) diammonium salt] method, the methanol extracts of Solanum jasminoides, Solanum barbisetum and Solanum anguivi multiflora exhibited potent antioxidant activity with IC50 values 31.25 ± 0.35, 40.33 ± 0.57 and 54.33 ± 0.57 μg/ml, respectively. Solanum barbisetum also showed potent activity in DPPH [1,1-diphenyl-2-picryl hydrazyl] method with an IC50 value of 55.33 ± 1.66 μg/ml. In the cytotoxicity studies, the methanol extract of Solanum barbisetum exhibited moderate activity against Vero, HEp-2, HeLa and A-549 cell lines with IC50 values in the range of 83.30 - 127.30 μg/ml. Solanum anguivi multiflora extract also showed moderate activity against Hep-2 cell line with IC50 value of 80.13 μg/ml. Solanum barbisetum possessing both the activities requires further investigation. The aim of the study was to evaluate in vitro antioxidant and cytotoxic activities of the methanolic extracts of leaves of Solanum sisymbrifolium, Solanum anguivi multiflora, Solanum barbisetum and Solanum jasminoides. In the in vitro antioxidant screening using ABTS [2,2’-azino-bis (3-ethylbenzo-thiazoline-6- sulphonic acid) diammonium salt] method, the methanol extracts of Solanum jasminoides, Solanum barbisetum and Solanum anguivi multiflora exhibited potent antioxidant activity with IC50 values 31.25 ± 0.35, 40.33 ± 0.57 and 54.33 ± 0.57 μg/ml, respectively. Solanum barbisetum also showed potent activity in DPPH [1,1-diphenyl-2-picryl hydrazyl] method with an IC50 value of 55.33 ± 1.66 μg/ml. In the cytotoxicity studies, the methanol extract of Solanum barbisetum exhibited moderate activity against Vero, HEp-2, HeLa and A-549 cell lines with IC50 values in the range of 83.30 - 127.30 μg/ml. Solanum anguivi multiflora extract also showed moderate activity against Hep-2 cell line with IC50 value of 80.13 μg/ml. Solanum barbisetum possessing both the activities requires further investigation.

Cytotoxic and antioxidant properties of four plants belonging to the genus Solanum

Dongre, Santoshkumar H.,Badami, Shrishailappa,Godavarthi, Ashok,S., Mahendran,P., Vijayan,B., Suresh Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.1

The aim of the study was to evaluate in vitro antioxidant and cytotoxic activities of the methanolic extracts of leaves of Solanum sisymbrifolium, Solanum anguivi multiflora, Solanum barbisetum and Solanum jasminoides. In the in vitro antioxidant screening using ABTS [2,2'-azino-bis (3-ethylbenzo-thiazoline-6-sulphonic acid) diammonium salt] method, the methanol extracts of Solanum jasminoides, Solanum barbisetum and Solanum anguivi multiflora exhibited potent antioxidant activity with $IC_{50}$ values 31.25 $\pm$ 0.35, 40.33 $\pm$ 0.57 and 54.33 $\pm$ 0.57 ${\mu}g$/ml, respectively. Solanum barbisetum also showed potent activity in DPPH [1,1-diphenyl-2-picryl hydrazyl] method with an $IC_{50}$ value of 55.33 $\pm$ 1.66 ${\mu}g/ml. In the cytotoxicity studies, the methanol extract of Solanum barbisetum exhibited moderate activity against Vero, HEp-2, HeLa and A-549 cell lines with $IC_{50}$ values in the range of 83.30 - 127.30 ${\mu}g$/ml. Solanum anguivi multiflora extract also showed moderate activity against Hep-2 cell line with $IC_{50}$ value of 80.13 ${\mu}g$/ml. Solanum barbisetum possessing both the activities requires further investigation.

Constitutive Behaviour of Fine Grained Soils of Vijayawada Region

Sateesh Narepalem,Venkata Rama Subba Rao Godavarthi 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.6

A comprehensive study was carried out to examine the suitability of hyperbolic model to establish undrained stress–strain (σ-ε) response of cohesive soils of Vijayawada region, India. The majority of locations in this region covered with cohesive soils. Standard penetration test (SPT) is the most commonly used in-situ test conducted as a part of geotechnical investigation in this region. In this paper a simplified hyperbolic model was proposed to evaluate the σ-ε curve in terms of SPT value (N value). The present study aims on predicting undrained shear strength/undrained cohesive strength (Cu) and elastic modulus (E) from N value. Empirical correlations would permit consulting engineers to rapidly estimate Cu and E using N value.

A Novel Herbal Formulation “LiverCare” Differentially Regulates Primary Rat Hepatocyte and Hepatocarcinoma Cell Proliferation In Vitro

Satyakumar Vidyashankar,Sandeep R. Varma,Mohammed Azeemudin,Ashok Godavarthi,Nandakumar S. Krishna,Pralhad Sadashiv Patki 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.9

Hepatocyte growth factor (HGF) plays an important role in hepatocyte proliferation. HGF expression is regulated by various signaling molecules and nuclear receptors. In the present study, LiverCare^ⓡ (LC), a novel polyherbal formulation (The Himalaya Drug Company, Bangalore, India), was evaluated for its efficacy, using co-cultures of primary rat hepatocytes–non-parenchymal cells (NPCs) and human hepatocellular carcinoma cells (HepG2). The rate of primary hepatocyte co-culture proliferation was significantly and dose-dependently increased by LC as determined by [3H]thymidine incorporation into newly synthesized DNA and cell proliferation assay. LC also increased HGF expression in primary hepatocyte co-culture. Albumin and urea content remained constant during proliferation of hepatocyte co-cultures in the presence of LC with decreased activity of alanine aminotransferase. It is interesting that LC inhibited incorporation of [3H]thymidine into DNA in HepG2 cells. LC enhanced peroxisome proliferator-activated receptor-α expression during hepatocyte proliferation, whereas tumor necrosis factor-α expression remained unaffected. In conclusion, our study clearly showed that LC differentially regulates primary rat hepatocytes and human hepatocarcinoma cell proliferation. LC may be a promising candidate for treating degenerative liver diseases by enhancing liver regeneration.

Hepatoprotective activity of methanol extracts of Berberis tinctoria

P, Vijayan,HC, Prashanth,Vijayaraj, Preethi,H, Raghu Chandrashekhar,Godavarthi, Ashok,SA, Dhanaraj Kyung Hee Oriental Medicine Research Center 2006 Oriental pharmacy and experimental medicine Vol.6 No.1

The methanol extracts of the roots, root bark and stem of Berberis tinctoria, were investigated for their hepatoprotective activity against carbon tetrachloride $(CCl_4)$ induced toxicity in freshly isolated rat hepatocytes, HEp-G2 cells and animal models. The methanol extracts were able to significantly normalise the levels of aspartate amino transferase, alanine aminotransferase, alkaline phosphatase, triglycerides, total proteins, albumin, total bilirubin and direct bilirubin, which were altered due to $CCl_4$ intoxication in freshly isolated rat hepatocytes and also in animal models. The anti-hepatotoxic effect of the methanol extracts in vitro were observed at $600\;-\;1,000\;{\mu}g/ml$ concentrations. A dose dependent increase in the percentage viability was observed when $CCl_4$ exposed HEp-G2 cells were treated with different concentrations of the methanol extracts. The highest percentage viability of HEp-G2 was observed at a concentration of $1,000\;{\mu}g/ml$. The results from the present investigations also indicate good correlation between the in vivo and in vitro studies.