運動負荷前後 肥滿女中生의 血中 Glucose, Insulin 및 Apolipoprotein 代謝變化

김태운,신군수,김종인,이광무,김성헌,안병철,고기준 釜山大學校 附設 體育科學硏究所 1994 體育科學硏究所 論文集 Vol.10 No.-

The purpose of this study was to determin a study on the change of blood glucose, insulin and apolipoprotein metabolism before and after maximal exercise in obese middle School girls. The subjects were included 2 groups (each group n=6) : obese group, non-obese group. The measurement variables were blood glucose, insulin and apolipoprotein before and after maximal exercise. The conclusion of this study was as follows: 1. Changes in glucose 1) Obese group had no significant differences by maximal exercise. 2) Non-obese group has been increased significantly by maximal exercise (P<.05). 3) No significant difference was found between two groups. 2. Changes in insulin 1) Obese group had non-obese group had no significant differences by maximal exercise. 2) Obese group has been increased significantly when comparing with non-obese group (P<.05). 3. Changes in apoliporprotein 1) Two groups in Apo-AI has been increased significantly pre and post maximal exercise(P<.05). 2) No significant difference on Apo-AI was found between two groups. 3) Obese group and non-obese group in Apo-B had no significant differences by maximal exercise. 4) No significant difference on Apo-B was found between two groups.

가축분뇨를 이용한 SCP 생산 균주의 분리 및 균체 단백질 생산

한석균,고유석,안태영,배동훈 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

질소원으로서 계분을 이용하는 균주를 선별하고 계분배지에서 균체의 생육속도가 다른 균주에 비하여 우수한 균주를 분리하였다. 형태·생리학적 특성을 기초로 하여 yeast의 분류 기준과 비교하여 본 균주를 Candida sp.로 동정하였으며 본 균주를 Candida sp. D116으로 명명하였다. Poultry feces extract medium에서 4% 농도의 glucose 첨가가 균체 생육에 효과적이었다. D116 균주를 액체 발효하여 균체생산능, 요산 그리고 가용성 단백질의 변화를 조사하였다. 그 결과 약 60시간이 경과하면 액체 발효 배지내의 거의 모든 가용성 단백질 및 요산의 감소를 보였으며 균체생육은 약 36시간 배양하였을 때 최고조에 도달하였고 그 후에는 점차 감소하는 경향을 보였다. SCP의 대량생산의 결과 50%의 계분혼합 배지와 30℃의 배양 온도에서 36시간 배양하여 균주의 생육수준이 3.8×10^9 CFU/ml 농도의 균체를 생산하였고 200 L의 배양액중 약 870 g-dw의 균체를 얻었으며 생산된 군체의 조단백질 함량은 67%이었다. Production of Single Cell Protein from Poultry Feces. Suk-kyun Han, You-Suk Go, Tae-Young Ahn and Dong-Hoon Bal^1*. Deparment of Microbioligy, College of Natural Sciences, Dankook Univerity, Cheonan 330-714 and Research Center for Molecular Microbiology, Seoul Nationa University, Seoul 151-742, Korea, ^1Department of Food Engineering. College of Engineering, Dankook University, Cheonan 330-714 and Bioproducts Research Center of Yonsei University, Seoul 120-749, Korea - From the soil collected form provincial area of South Korea, a microorganisms which have been shown good growth in the minimal poultry feces extract medium was isolated. Supplement of glucose to the poultry feces extract medium helped the complete degraded during the microbial growth. Maximum cell growth (3.8×10^9 CFU/ml) obtained at 36 hours of incubation after inoculation. Uric acid was degraded faster in minimal medium that in the glucose complement medium. VFA (volatile fatty acid), which are known as major compounds of poultry feces odor, were almost removed from the minimal poultry feces extract medium. Glucose supplement to the minimal medium enhanced the growth of microbial cells. Addition of 4% of glucose and 4% of neopeptone to the minimal poultry feces extract medium helped the maximal growth of cells.

BAM(Brewster Angle Microscope)으로 관측한 Langmuir막의 상전이에 따른 분자 배향에 관한 연구

조환제,강도원,박태곤 창원대학교 산업기술연구소 2001 産技硏論文集 Vol.15 No.-

In this study, we used Brewster-Angle Microscope(BAM) to study on the molecular orientation of monolayer on the water surface. The domains by behaviors of molecules on three different subphase have been observed. Reproducible π-A isotherm have been obtained only on information about phase transition by molecular area. BAM facilitates the observation of morphology by optical anisotropy and thickness in monolayer and multilayers as BAM is shown to be sensitive to anisotropy of film. Also, the coalescence of domains leads to large regions of uniform tilt-azimuthal direction. Every transition was found by BAM technique, either as a dramatic change in degree of contrast or as a sudden alteration of molecular action and π-A isotherm.

補虛湯이 免疫機能에 미치는 影響

고희경,남경수,이태균 동국대학교 경주대학 1995 東國論集 Vol.14 No.-

For the purpose of investigate the immunoadjuvant effects of BoheuTang, BoheuTang was pretreated to Balb/c mice for 10 days with intraperitoneally. After 10 days, haptenic antigen (VCR-BSA, vincristine-bovine serum albumin) complexed with Freund's complete adjuvant was immunized. The immunoadjuvant were followed after 7 days of first immunization. The immunoadjuvant experimentswere as follows : 1. The antibody production in Balb/c mouse immunized with VCR-BSA was remarkably increased in the pretreated group of BoheuTang compared with saline treated(control) group. 2. The macrophage numbers in Balb/c mouse immunized with VCR-BSA was considerably increased about 2.9 times in the pretreated groups of BoheuTang compared with control group. 3. The lysozyme activity of macrophage in Balb/c mouse that was immunized with VCR-BSA was increased gradually in the pretreated groups of BoheuTang compared with control group. 4. In the BoheuTang pretreated group, B cell(spleen cell) proliferation was increased with a dose dependent manner. At 20μl/well of BoheuTang, B cell proliferation activity was most highest. 5. Onthe other hand, in the T cell(thymocyte) proliferation BoheuTang pretreated group had less considerable activity than in the case of B cell. These results may suggest that BoheuTang will have immunoadjuvant effects on the cells which concerned with immuno mechanism.

제주도 주변 용승역의 생물생태학적 기초 연구(I)-제주도 남서부 연안해역의 해수화학적 특성과 용승현상

고유봉,최영찬,강태연 濟州大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.10 No.1

1) 이 연구해역의 밀도 (δt), 영양염류의 수직·수평분포도로 부터 연안용승현상을 볼수 있었다. 4월은 상충용승형이 미미하게 나타나고 있고, 6∼11월은 상·중층에서 등농도선이 해수면으로 상승하고, 중·하층에서 해저면으로 하강하는 형태의 상하분산형 용승이 뚜렷하게 나타났다. 용승은 6월과 11월에 강하게 나타났으며, 폭도 넓게 형성되었다. 2) 용승지역의 영양염류의 표층 농도범위는 질산질소가 0.06∼7.12㎍-at/ℓ, 인산인이 0.03∼1.75㎍-at/ℓ, 규산규소가 2.75∼22.32㎍-at/ℓ를 나타내어 계절과 수심에 따라 큰 차이를 보였다. 상하혼합이 잘 이루어지는 겨울과 초봄에 고농도의 영양염류가 균일하게 분포하고 있고, 여름과 가을철 표층에서 낮고 수심에 따라 증가하였다. 또한 용승해역인 연안역의 정점 1과 2의 표층수에서 영양염의 농도는 바깥쪽 해역보다 현저하게 높게 나타나고 있다. 3) 용존산소농도는 표층(0∼30m)에서 년평균 5.28㎖/ℓ, 저층(60∼100m)에서 7.30㎖/ℓ를 나타내어 특이하게 저층에서 높았다. 이는 황해냉수괴의 영향인지 아니면 다른 요인이 있는 것인지 면밀한 조사가 요구된다. 또 다른 특징은 8∼11월에 22∼56㎍-at/ℓ 범위의 고농도 규산규소가 전 정점에 폭넓게 분포하고 있는데 이는 봄철 대증식으로 발생한 플랑크톤의 분해와 양자강연안수의 영향으로 보아진다. 4) 이 지역의 년평균 N/P비는 8.0, Si/P비는 46.3으로 나타나 제주도 북부, 남부, 동부와 비교하여 낮은 N/P값과 높은 Si/P값을 보였다. 5) 식물색소의 농도범위는 0.04∼2.36㎍/ℓ로 특히 봄철에 표층수에서 2㎍/ℓ 이상의 높은값을 나타내었고, 특히 용승해역인 연안역이 바깥쪽 해역보다 높게 나타났다. 6) 부유물질의 평균값은 3.14㎎/ℓ(0.75∼8.47㎎/ℓ)로 생물의 서식하기에 호적한 상태였고, VSS/SS백분율은 연안역의 정점 1에서 53%, 바깥쪽 해역의 정점 5에서 46%로 나타났으며 수심증가에 따라 감소하였다. 또한 연안역의 바깥쪽 해역보다 휘발성분이 높고, 표층보다 심층으로 갈수록 고형성분이 증가하였다. Hydrographic conditions (temperature, salinity, dissolved oxygen), nutrients, chlorophyll-a and suspended solid have been studied in the southwestern coastal area of Cheju Island from April 1993 to March 1994. Vertical profile of temperature, salinity and chemical properties (nutrients, chlorophyll-a) distribution in th southwestern sea of Cheju Island showed a upwelling feature. Although it was not clear in winter season, it seems to continued through out the year. In the surface water at the upwelling areas, the ranges of dissolved oxygen, nitrate, phosphate and silicate was 3.30∼8.43 ㎖/ℓ, 0∼7.12 ㎍/ℓ, 0.03∼1.75 ㎍-at/ℓ and 2.75∼22.32 ㎍-at/ℓ, respectively. Nutrients was higher in the shore water than in the offshore water, because sufficient supply of nutrients from the bottom water by coastal upwelling. In November, especially high concentration silicate was observed at all the stations and depth in the study area. At all station of bottom water (down to the depth of 60 meter), concentration value of dissolved oxygen was as high as 8 ㎖/ℓ. Mean values of N/P was 8.0, lower than Redifield ratio of 16. The mean values of Si/P was observed to 46.3 in southwest of Cheju Island. Concentration of chlorophyll-a was in the range of 0.04∼2.36 ㎍/ℓ. Concentration of chlorophyll-a in surface water at all station was especially higher in spring than in other seasons. Mean concentration value of suspended solids was 3.14 ㎎/ℓ (0.75∼8.47 ㎎/ℓ). Ratio of the volatile suspended solids to the suspended solids was higher in the inshore water (53%) than in the offshore water (46%), and higher in the surface water than in the bottom water.

제주도 연안역 부유성 요각류의 출현과 주요종의 계절변화

고유봉,오봉철,고방연,손태준 제주대학교 해양연구소 1994 해양자원연구소연구보고 Vol.18 No.-

상 요추 추간판 탈출증의 수술적 치료

조태현,송준혁,김명현,박향권,김성학,신규만,박동빈 梨花女子大學校 醫科大學 醫科學硏究所 2000 EMJ (Ewha medical journal) Vol.23 No.3

Objectives : Lumbar disc herniations at the L1-L2, L2-L3, and L3-L4 level are rare. The purpose of this study is to observe the variable clinical finding of upper lumbar disc herniations and to provide proper treatment modality. Patients and Methods : Between 1998 Jan. and 1999 Dec., seventeen patients with upper lumbar disc herniations who were undergone surgery in our institute were retrospectively evaluated. Patients were evaluated based on following factors : age, sex, aggravating factor, symptoms and signs type of disc herniation, type of surgery, and outcome of post-surgery. Results : The incidence of lumbar disc herniation at the level of L1-2, L2-3 and L3-4 is 6.1% with declining frequency as the level ascends. The peak age incidence is 6th decade in both sex. Preoperative symptomes and signs are variabe. In sixteen cases, posterior approach was done. In these cases, twelve cases were performed microscopic partial hemilaminectomy, and four cases with spinal stenosis were performed total laminectomy and posterior screw fixation with bone fusion. In one case, lateral extracavitary approach was done. In results of operation, fourteen cases showed more than good grade(82%). Conclusion : The age incidence of lumbar disc herniations at the level of L1-2, L2-3 and L3-4 older than lumbar disc herniations at the lower level of L4-5 and L5-S1. The signs and symptoms are variable. In our cases, most of the patients were performed posterior approach with microscopic partial hemilaminectomy except the patients combined spinal stenosis. One case was performed a lateral extracavitary approach because the risk of the cord and cauda equina injuries. The prognosis of upper lumbar disc herniations after treatment with only microscopic partial hemilaminectomy and diskectomy is comparable with the prognosis of lumbar disc herniations at the lower level.

제주도 연안역 부유성 요각류의 출현과 주요종의 계절변화

손태준,오봉철,고유봉,고방연 濟州大學校海洋硏究所 1994 해양과환경연구소 연구논문집 Vol.18 No.-

Seasonal fluctuations of species composition and abundance of pelagic copepods on the coast of Cheju Island were examined. Samples were collected from the four surface waters(Udo, Gosan, Seogwi and Cheju) of Cheju Island during the period from July 1990 to March 1994. There was considerable seasonal variation in occurance of pelagic copepods, with high values from summer to fall and low ones from winter to spring. Mean copepodid individuals showed a peak in May 1991 and 1992. Numerical abundance of copepods was observed in May and November. Copepodid and other crustacean larvae gave the evidence of active production during the period of sampling. Seventy seven species of pelagic copepods were identified, of which Paracalanus indicus, Acartia omorii, A. steueri, Oncaea venusta, Oithona plumifera, Calanus sinicus, Corycaeus affinis were main constituents. Most of these copepods are well found in Kuroshio current. P. indicus showed three peaks(early spring, early summer and early winter) in adult individuals, C. sinicus, A. steueri and O. plumifera, two peaks in winter and summer, C. affinis two peaks in spring and winter, A. omorii showed only one peak in autumn in this study area.

비만 여중생과 정상 여중생의 혈중지질 비교분석

김태운,신군수,김종인,이광무,김성헌,안병철,고기준 釜山大學校 附設 體育科學硏究所 1995 體育科學硏究所 論文集 Vol.11 No.-

The purpose of this study was to analyze and identify the serum lipids of the middle school girls in order to utilize them as the basic data for the prevention of the middle school girls against arteriosclerosis and diabetes tending to lower age. We used total of twelve middle school girls as subject and devided them into two groups : obese group(n=6), normal group(n=6). The measurement items were the Total-cholesterol, HDL-cholesterol, LDL-cholesterol, TG, FFA. The conclusion of this study was as follows : 1. Total-cholesterol 1) All two groups was a little increased by maximal exercise, but there was no significant difference statistically. 2) Normal group was a little higher than obese group, but there was no significant difference. 2. HDL-cholesterol 1) All two groups was a little increased by maximal exercise, but there was no significant difference. 2) In comparison of two groups, normal group was a little high on empty stomach and obese group was a little high before and after exercise, but there was no significant difference. 3. LDL-cholesterol 1) All two groups was a little increased on post-exercise than pre-exercise, but there was no significant difference statistically. 2) In comparison of two groups, normal group was a little higher than obese group, but there was no significant difference. 4. Triglyceride 1) All two groups was a little increased by maximal exercise, but there was no significant difference. 2) In comparison of two groups, normal group was a little higher than obese group on empty stomach and obese group was a little higher than normal group on pre and post-exercise, but there was no significant difference. 5. T-chol./HDL-chol. 1) All two groups was a little increased by maximal exercise, but there was no significant difference. 2) In comparison of two groups, normal group was a little higher than obese group, but there was no significant difference. 6. Free Fatty Acid 1) All two groups was a little decreased by maximal exercise, but there was no significant difference. 2) In comparison of two groups, obese group was a little higher than normal group, but there was no significant difference.

전자유압 서보 유량제어밸브의 설계 및 성능에 관한 연구

김수태,김고도 國立 昌原大學校 産業技術硏究所 1999 産技硏論文集 Vol.13 No.-

An experimental and theoretical analysis for the improvement of dynamic characteristics and design of electro-hydraulic flow control servo valve are performed. The theoretical results are compared with the experimental step responses and important design parameters of an electro-hydraulic flow control servo valve are derived by using simulation program. Simulation parameters of nozzle jet coefficient and orifice and spool valve discharge coefficient are obtained by experiment. The results of this study are summarized as follows. 1. Discharge coefficient of small orifices and nozzles (d=0.20∼0.55㎜) are obtained by experiment. The experimental curves show the discharge coefficient change from 0.6 to 0.78. 2. Valve gain depends on R?? value (fixed orifice and nozzle ratio) and theoretical and experimental step response curves show that the valve gain is maximum at R?? = 1 3. Drain orifice in the flapper nozzle return line creates a small back pressure, which improves the performance for the valve. However R?? valve should be larger than 2 because drain orifice decreases valve gain and make valve unstable. 4. The optimum R?? value of the tested valve is 4 in the condition R?? = 1.