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Review : Biological Engineering ; A Review on Lateral Flow Test Strip for Food Safety
( Giyoung Kim ),( Jongguk Lim ),( Changyeun Mo ) 한국농업기계학회 2015 바이오시스템공학 Vol.40 No.3
Background: Foodborne disease outbreaks from various food sources are a major health concern worldwide. Current methods for detection of foodborne pathogens are both expensive and time-consuming. Purpose: This review aims to present the current information available on the use of lateral flow test strips to detect pathogens in food products to enhance food safety. Results: Frequent foodborne disease outbreaks from various food sources have increased the need for rapid and easy methods for routine analysis of foodborne pathogens. Present detection methods for foodborne pathogens require expensive instruments, experts, and long time for sample analysis. Lateral flow test strips have drawn attention in recent years because of their ability to detect analytes quickly and easily. This review focuses on the principle of the lateral flow test, the various formats of lateral flow test strips, recognition elements, labeling tags, and reading instruments. In addition, this review also discusses the future prospects for the lateral flow test strips.
A Simple and Facile Screening Method to Discover the Plastic Degrading Microorganisms
Giyoung SHIN,Hyuni JUNG,Sung Yeon HWANG,Jeyoung PARK,Dongyeop X. OH 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The current decomposition rate of biodegradable plastics cannot satisfactorily curb the global plastic waste crisis. Thus, identifying efficient plastic-digesting microorganisms is necessary to accelerate the decomposition of plastics. However, screening for these microorganisms is labor-intensive and time-consuming. The distribution of hydrophobic plastics homogeneously, with a high surface area and cell accessibility, in a culture medium is challenging. Herein, a high-throughput, straightforward micro-spray-based screening system is presented. Plastic microparticles are sprayed on an agar plate, thereby providing a translucent screening plate. This method is rapid, as opposed to the tedious, intricate, and skilled preparation required for polymer emulsion. Micro-sprayed plastic samples require a minimal effective dose (15 ㎎) and share a large interface with microorganisms. The plastic-degrading activity of microbes results in a transparent clear zone around the colony, thereby being readily recognizable. Our method can accelerate the discovery of novel plastic-degrading microorganisms and be applied to future studies on the decomposition of non-degradable plastics.
Applications of Microfluidics in the Agro-Food Sector: A Review
( Giyoung Kim ),( Jongguk Lim ),( Changyeun Mo ) 한국농업기계학회 2016 바이오시스템공학 Vol.41 No.2
Background: Microfluidics is of considerable importance in food and agricultural industries. Microfluidics processes low volumes of fluids in channels with extremely small dimensions of tens of micrometers. It enables the miniaturization of analytical devices and reductions in cost and turnaround times. This allows automation, high-throughput analysis, and processing in food and agricultural applications. Purpose: This review aims to provide information on the applications of microfluidics in the agro-food sector to overcome limitations posed by conventional technologies. Results: Microfluidics contributes to medical diagnosis, biological analysis, drug discovery, chemical synthesis, biotechnology, gene sequencing, and ecology. Recently, the applications of microfluidics in food and agricultural industries have increased. A few examples of these applications include food safety analysis, food processing, and animal production. This study examines the fundamentals of microfluidics including fabrication, control, applications, and future trends of microfluidics in the agro-food sector. Conclusions: Future research efforts should focus on developing a small portable platform with modules for fluid handling, sample preparation, and signal detection electronics.
Biological Engineering : Review ; Review on Biosensors for Food Safety
( Giyoung Kim ),( Ji Hea Moon ),( Jongguk Lim ),( Changyeun Mo ) 한국농업기계학회 2014 바이오시스템공학 Vol.39 No.2
Background: Frequent outbreaks of foodborne illness have been increased awareness of food safety. CDC estimates thateach year roughly 48 million people gets sick, 128,000 are hospitalized and 3,000 die of foodborne diseases in US. In Korea,6,058 were hospitalized and 266 incidents were reported in 2012. It is required to develop rapid methods to identify hazardsubstances in food products for protecting and maintaining safety of the public health. However, conventional methods forpathogens detection and identification involve prolonged multiple enrichment steps. Purpose: This review aims to provideinformation on biosensors to detect pathogens in food products to enhance food safety. Results: Foodborne outbreakscontinue to occur and outbreaks from various food sources have increased the need for simple, rapid, and sensitive methodsto detect foodborne pathogens. Conventional methods for foodborne pathogens detection require tremendous amount oflabor and time. Biosensors have drawn attentions in recent years because of their ability to detect analytes sensitively andrapidly. Principles along with their advantages and disadvantages of a variety of food safety biosensors including fiber opticbiosensor, impedimetric biosensor, surface Plasmon resonance biosensor, and nano biosensor were explained. Also, futuretrends for the food safety biosensors were discussed.