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구연 : 간세포암에서 암세포 증식에 관련된 RPL36A 유전자 과발현
( Dae Ghon Kim ),( Kyung Ran You ),( Seung Ok Lee ),( Soo Taek Lee ),( Deuk Soo Ahn ),( Baik Whan Cho ),( Myung Ja Jung ) 대한간학회 2003 Clinical and Molecular Hepatology(대한간학회지) Vol.9 No.3(S)
Background/Aims: Hepatocellular carcinoma (HCC) is a worldwide major malignancy with an increasing incidence. Altered gene expression caused either by mutations or by changes in the regulatory characteristics of HCC compared with corresponding nontumor tissues have been reported. However, these genetic changes reported do not precisely reflect the biological nature or clinical characteristics of all HCCs. Methods: Previously, we identified and reported genes that were differentially expressed in HCC cells using the differential-display PCR method. Among them, we focused preferentially on an up-regulated gene in human HCC in comparison with the nontumor surrounding tissues. We identified a single fragment cDNA (HG23T1) that was over-expressed in a hepatocellular carcinoma (HCC) specimen. We cloned the full-length HG23T1 gene by the rapid amplification of cDNA end (RACE) PCR method. Results: It perfectly matched the gene encoding human ribosomal protein L36A (RPL36A or referred to RPL44). RPL36A mRNA was preferentially over-expressed in 34 of 40 HCC cases (85%, P <0.001) and in all 8 HCC cell lines. Ectotopically over-expressed L36A ribosomal protein localized in the nucleoli of cells and this localization seemed to be controlled by the N-terminal or the internal tetrapeptide consensus with its adjacent N-terminal domain. Over-expression of L36A led to enhanced colony formation and cell proliferation, which may have resulted from rapid cell cycling, and an antisense cDNA effectively reversed these alterations. Conclusions: These results suggest that RPL36A plays a role in tumor cell proliferation and may be a potential target for anticancer therapy of HCC.
대한간학회지 제8차 춘계학술대회 초록집 : 구연 ; 담관세포암 육종성변화 관련 발현유전자 cDNA Microarray 분석
( Dae Ghon Kim ),( Young Tae Kim ),( Kyung Ran You ),( Win Jing ),( Deuk Soo Ahn ),( Jung Min Lee ),( Jeong Min Lee ),( Hee Chul You ),( Baik Whan Cho ),( Jung Kyoon Choi ),( Sang Soo Kim ) 대한간학회 2002 Clinical and Molecular Hepatology(대한간학회지) Vol.8 No.2(S)
Downregulation of annexin A8 (ANXA8) during sarcomatous change of cholangiocarcinoma
( Dae Ghon Kim ),( Kyung Ran You ),( Hyo Jung Kim ),( Woo Sung Moon ),( Baik Whan Cho ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-
<Aims> Cholangiocarcinoma (CC) is one of the most aggressive and fatal maligancies. Furthermore, molecular cholangiocarcinogenesis and sarcomatous change/epithelial mesenchymal transition (EMT) of CC is unclear. <Materials and methods> We examined the exp