A Novel Method for Preparing of Oxoruthenates Complexes: trans-[RuO3(OH)2]2-, [RuO4]-, (n-Pr4N)+[RuO4]- and [RuO4] and Their Use as Catalytic Oxidants

Abdel-Ghany F. Shoair 대한화학회 2005 Bulletin of the Korean Chemical Society Vol.26 No.10

The synthesis and characterization of K3[Ru(C2O4)3]•4H2O (C2O42 = oxalato anoin) complex are described, and its redox properties (in buffer solution of pH = 12) have been investigated. This complex is used for in situ generation of oxoruthenates complexes which have been characterized by electronic spectroscopy. Reaction of K3[Ru(C2O4)3]•4H2O with excess S2O82 in molar KOH generates trans-[RuO3(OH)2]2/S2O82 reagent while with excess BrO3 in molar Na2CO3 generates [RuO4]/BrO3 reagent. Avoiding the direct use of [RuO4] the organic-soluble (n-Pr4N)+[RuO4], (TPAP) has been isolated by reaction of K3[Ru(C2O4)3]•4H2O with excess BrO3 in molar carbonate and n-Pr4NOH. In a mixture of H2O/CCl4 ruthenium tetraoxide can be generated by reaction of K3[Ru(C2O4)3]•4H2O with excess IO4. The catalytic activities of oxoruthenates that have been made from K3[Ru(C2O4)3]•4H2O towards the oxidation of benzyl alcohol, piperonyl alcohol, benzaldehyde and benzyl amine at room temperature have been studied.

Characterizations of nano-zinc doped hydroxyapatite to use as bone tissue engineering

Abdel-Ghany, Basma E.,Abdel-Hady, Bothaina M.,El-Kady, Abeer M.,Beheiry, Hanan H.,Guirguis, Osiris W. Techno-Press 2015 Advances in materials research Vol.4 No.4

Contamination by bacterial strands is a major problem after bone replacement surgeries, so there is a great need to develop low cost biocompatible antibacterial bioactive scaffolds to be used in bone tissue engineering. For this purpose, nano-zinc doped hydroxyapatite with different zinc-concentrations (5, 10 and 15 mol%) was successfully prepared by the wet chemical precipitation method. The prepared powders were used to form porous scaffolds containing biodegradable Ca-cross-linked alginate (5%) in order to enhance the properties of alginate scaffolds. The scaffolds were prepared using the freeze-gelation method. The prepared powders were tested by X-ray diffraction; transmission electron microscope and Fourier transform infrared analyses, while the prepared scaffolds were investigated by Fourier transform infrared analyses, thermogravimetric analyses and measurement of the antibacterial properties. Best results were obtained from scaffold containing 15% mol zinc-doped hydroxyapatite powders and 5% alginate concentration with ratio of 70:30.

SPECIAL LECTURE 2 : Hepatitis C Virus Infection in Public Health

( Marc Ghany ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Hepatitis C is a global public health issue. It is estimated that 170-190 million persons are chronically infected worldwide. Chronic HCV infection is a major cause of cirrhosis, haptic decompensation, hepatocellular carcinoma and liverrelated death. The prevalence of chronic hepatitis C varies widely by geographic region from a low of .003% in developed Western countries to a high of 14% in Egypt and several African countries. The incidence of hepatitis C is declining in developed countries due to the introduction of educational programs and screening of blood products but remains high in in developing countries with moderate to high prevalence. The most important modes of transmission worldwide are injection drug use and unsafe medical practice. Persons at high risk for chronic hepatitis C should be screened (persons who have ever injected drugs, recipients of blood transfusion or solid organ transplant before 1992, recipients of blood or organs from donors who later tested HCV positive, recipients of clotting factor concentrates manufactured before 1987, persons who have ever received long-term hemodialysis, all persons with HIV infection, persons with unexplained aminotransferase elevations and children born to women infected with hepatitis C). Recently, the Centers for Disease Control and Prevention has recommended screening individuals born between 1945 and 1970 based on data demonstrating that it is more cost-effective than risk-based screening. Screening strategies can only be effective if linked to education to prevent transmission, care and access to therapy. Although therapy is becoming more effective and is associated with improved outcomes, only a minority of subjects with chronic hepatitis C receives therapy. Understanding the barriers to therapy is important for reducing the morbidity and mortality associated with chronic hepatitis C infection. Recognized barriers include patient, physician, government and payer-related related factors. Greater efforts are needed not only by public health agencies, to implement national strategies to prevent, control and treat hepatitis C infection but also by private practioners, hospitals and other clinics, particularly since an effective vaccine is unlikely in the foreseeable future.

SYMPOSIUM 4 : Current Management of HCV Infection

( Marc Ghany ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

The therapeutic landscape of chronic hepatitis C is rapidly changing with the development of potent inhibitors of viral and host proteins necessary for viral replication. Two currently approved protease inhibitors have significantly improved the response rate in genotype 1 subjects with chronic hepatitis C but still require the use of peginterferon and ribavirin. Thus, current therapy of genotype 1 infection is a triple drug regimen consisting of a protease inhibitor-either boceprevir or telaprevir in combination with peginterferon and ribavirin administered for either 24 or 48 weeks depending on initial virological response and prior treatment history. The approved direct acting antiviral agents are not effective against other genotypes. Hence, therapy of genotypes 2 and 3 is the combination of peginterferon and ribavirin, at a reduced dose of 800 mg daily, for 24 weeks. Therapy of genotypes 4-6 is the combination of peginterferon and ribavirin (dosed based on weight) for 48 weeks. The strongest pre-treatment predictors of a response to therapy are a subject`s IL28B genotype, viral genotype and severity of underlying disease. How rapidly the virus is eliminated is the best on-treatment predictor of response and is superior to pre-treatment predictors in determining response to therapy. Future therapy is rapidly evolving towards interferon-free regimens for all genotypes with shorter durations of therapy and better safety profiles. These advancements hold the promise of curative therapy for a substantial proportion of subjects with chronic hepatitis C infection.

A Novel Method for Preparing of Oxoruthenates Complexes: trans-[RuO₃(OH)₂]^2-, [RuO₄]^-, (n-Pr₄N)⁺[RuO₄]^- and [RuO₄ and Their Use as Catalytic Oxi

Shoair, Abdel-Ghany F. Korean Chemical Society 2005 Bulletin of the Korean Chemical Society Vol.26 No.10

The synthesis and characterization of ${K_3[Ru(C_2O_4)3]{\cdot}4H_2O\;(C_2O_4}^{2-}$ = oxalato anoin) complex are described, and its redox properties (in buffer solution of pH = 12) have been investigated. This complex is used for in situ generation of oxoruthenates complexes which have been characterized by electronic spectroscopy. Reaction of ${K_3[Ru(C_2O_4)3]{\cdot}4H_2O$ with excess ${S_2O_8}^{2-}$ in molar KOH generates trans-${[RuO_3(OH)_2]^{2-}/S_2O_8}^{2-}$ reagent while with excess ${BrO_3}^-$ in molar $Na_2CO_3$ generates ${[RuO_4]^-/BrO_3}^-$ reagent. Avoiding the direct use of [$RuO_4$] the organic-soluble $(n-Pr_4N)^+[RuO_4]^-$, (TPAP) has been isolated by reaction of $K_3[Ru(C_2O_4)3]{\cdot}4H_2O$ with excess ${BrO_3}^-$ in molar carbonate and n-$Pr_4$NOH. In a mixture of $H_2O/CCl_4$ ruthenium tetraoxide can be generated by reaction of $K_3[Ru(C_2O_4)3]{\cdot}4H_2O$ with excess ${IO_4}^-$. The catalytic activities of oxoruthenates that have been made from $K_3[Ru(C_2O_4)3]{\cdot}4H_2O$ towards the oxidation of benzyl alcohol, piperonyl alcohol, benzaldehyde and benzyl amine at room temperature have been studied.

Health Disparities between Black Hispanic and Black Non-Hispanic Cervical Cancer Cases in the USA

Khan, Hafiz Mohammad Rafiqullah,Gabbidon, Kemesha,Abdool-Ghany, Faheema,Saxena, Anshul,Gomez, Esneider,Stewart, Tiffanie Shauna-Jeanne Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Globally, cervical cancer is a major public health concern. Cervical cancer is the second most common cancer among women, resulting in approximately 500,000 cases per year. The purpose of this study is to compare disease characteristics between Black Hispanic (BH) and Black non-Hispanic (BNH) women in the US. Materials and Methods: We used stratified random sampling to select cervical cancer patient records from the SEER database (1973-2009). We used Chi-square and independent samples t-test to examine differences in proportions and means. Results: The sample included 2,000 cervical cancer cases of Black non-Hispanic and 91 Black Hispanic women. There were statistically significant differences between black Hispanic and black non- Hispanics in mean age at diagnosis (p<0.001), mean survival time (p<0.001), marital status (p<0.001), primary site of cancer (p<0.001); lymph node involvement (p<0.001); grading and differentiation (p<0.0001); and tumor behavior (p<0.001). Black women were more likely to develop cervical cancer and to have the highest mortality rates from the disease. Conclusions: Findings from this study show clear racial and ethnic disparities in cervical cancer incidence and prognosis that should be addressed.

Big IoT Healthcare Data Analytics Framework Based on Fog and Cloud Computing

( Hamoud Alshammari ),( Sameh Abd El-ghany ),( Abdulaziz Shehab ) 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.6

Throughout the world, aging populations and doctor shortages have helped drive the increasing demand for smart healthcare systems. Recently, these systems have benefited from the evolution of the Internet of Things (IoT), big data, and machine learning. However, these advances result in the generation of large amounts of data, making healthcare data analysis a major issue. These data have a number of complex properties such as high-dimensionality, irregularity, and sparsity, which makes efficient processing difficult to implement. These challenges are met by big data analytics. In this paper, we propose an innovative analytic framework for big healthcare data that are collected either from IoT wearable devices or from archived patient medical images. The proposed method would efficiently address the data heterogeneity problem using middleware between heterogeneous data sources and MapReduce Hadoop clusters. Furthermore, the proposed framework enables the use of both fog computing and cloud platforms to handle the problems faced through online and offline data processing, data storage, and data classification. Additionally, it guarantees robust and secure knowledge of patient medical data.

Purification and Characterization of Anabaena flos-aquae Phenylalanine Ammonia-Lyase as a Novel Approach for Myristicin Biotransformation

Asmaa M. Arafa,Afaf E. Abdel-Ghany,Samih I. El-Dahmy,Sahar Abdelaziz,Yassin El-Ayouty,Ashraf S. A. El-Sayed 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4

Phenylalanine ammonia-lyase (PAL) catalyzes the reversible deamination of phenylalanine to cinnamic acid and ammonia. Algae have been considered as biofactories for PAL production, however, biochemical characterization of PAL and its potency for myristicin biotransformation into MMDA (3-methoxy-4, 5-methylenedioxyamphetamine) has not been studied yet. Thus, PAL from Anabaena flos-aquae and Spirulina platensis has been purified, comparatively characterized and its affinity to transform myristicin was assessed. The specific activity of purified PAL from S. platensis (73.9 μmol/mg/min) and A. flos-aquae (30.5 μmol/mg/min) was increased by about 2.9 and 2.4 folds by gel-filtration comparing to their corresponding crude enzymes. Under denaturing-PAGE, a single proteineous band with a molecular mass of 64 kDa appeared for A. flos-aquae and S. platensis PAL. The biochemical properties of the purified PAL from both algal isolates were determined comparatively. The optimum temperature of S. platensis and A. flos-aquae PAL for forward or reverse activity was reported at 30oC, while the optimum pH for PAL enzyme isolated from A. flos-aquae was 8.9 for forward and reverse activities, and S. platensis PAL had maximum activities at pH 8.9 and 8 for forward and reverse reactions, respectively. Luckily, the purified PALs have the affinity to hydroaminate the myristicin to MMDA successfully in one step. Furthermore, a successful method for synthesis of MMDA from myristicin in two steps was also established. Gas chromatography-mass spectrometry (GC-MS) analysis was conducted to track the product formation.

In vitro repress of breast cancer by bio-product of edible Pleurotus ostreatus loaded with chitosan nanoparticles

Al-Rajhi Aisha M. H.,Ghany Tarek M. Abdel 한국응용생명화학회 2023 Applied Biological Chemistry (Appl Biol Chem) Vol.66 No.-

Despite advances in early detection and therapy, cancer still is a significant health challenge with the highest priority for investigation. Breast cancer represents the most common cancerous disease among women in the world. The study’s purpose is to estimate the cytotoxic activity of the edible mushroom Pleurotus ostreatus extract (PE), chitosan nanoparticles (ChNPs), and PE loaded with ChNPs (PELChNPs), as well as to identify the molecular docking of the cytotoxicity of methyl gallate (MG) as a main component of the PE against breast cancer (MCF-7) cell line. High-performance liquid chromatography (HPLC) analysis of PE exhibited the existence of various phenolic and flavonoid compounds such as MG, gallic acid, chlorogenic acid, hesperetin, naringenin, rutin, and cinnamic acid. The proliferation of the MCF-7 cell line was inhibited at 1, 3.9, and 62.50 µg/mL of PELChNPs, PE, and ChNPs, respectively. PELChNPs were more effective against the MCF-7 cell line than PE, particularly at low concentrations. For instance, at 7.8 µg/mL of PELChNPs and PE, the inhibitory % of MCF-7 proliferation was 20.59±1.75% and 8.57±0.59%, respectively. At 15.6 µg/mL of PELChNPs and PE, the inhibitory % of MCF-7 proliferation was 51.37±1.09% and 25.18±1.64%, respectively. While there is slight difference in the inhibition % of MCF-7 cells (98.64±0.21 and 97.22±0.16%) at high concentration 500 µg/mL of PELChNPs and PE, respectively. IC50 was 15.25 ± 0.54 µg/mL, 46.27 ± 1.94 µg/mL, and 337.38 ± 13.68 µg/mL against MCF-7 cell line of PELChNPs, PE, and ChNPs, respectively. The value of IC50 documented the efficacy of PELChNPs compared with the IC50 (5.91 ± 0.43 µg/mL) of Vinblastine sulfate. Noticeable distortions were observed in the MCF-7 cell line mainly treated with PELChNPs, followed by PE alone. While ChNPs exhibited less effect on the morphology of the MCF-7 cell line. Antioxidant activity of ChNPs, PE, and PELChNPs was evaluated compared with Trolox, which reflected IC50 = 118.33 ± 4.02, 85.63 ± 3.96, 36.80 ± 2.52 and 24.74 ± 0.45 µg/mL. Methyl gallate binding interactions were assessed using molecular docking with the MOE-Dock tool against the target crystal structures of Breast cancer cell line 3HB5. The results shed light on how molecular modeling techniques can inhibit methyl gallate with possible uses in treating breast cancer.

Statistical Estimates from Black Non-Hispanic Female Breast Cancer Data

Khan, Hafiz Mohammad Rafiqullah,Ibrahimou, Boubakari,Saxena, Anshul,Gabbidon, Kemesha,Abdool-Ghany, Faheema,Ramamoorthy, Venkataraghavan,Ullah, Duff,Stewart, Tiffanie Shauna-Jeanne Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: The use of statistical methods has become an imperative tool in breast cancer survival data analysis. The purpose of this study was to develop the best statistical probability model using the Bayesian method to predict future survival times for the black non-Hispanic female breast cancer patients diagnosed during 1973-2009 in the U.S. Materials and Methods: We used a stratified random sample of black non-Hispanic female breast cancer patient data from the Surveillance Epidemiology and End Results (SEER) database. Survival analysis was performed using Kaplan-Meier and Cox proportional regression methods. Four advanced types of statistical models, Exponentiated Exponential (EE), Beta Generalized Exponential (BGE), Exponentiated Weibull (EW), and Beta Inverse Weibull (BIW) were utilized for data analysis. The statistical model building criteria, Akaike Information Criteria (AIC), Bayesian Information Criteria (BIC), and Deviance Information Criteria (DIC) were used to measure the goodness of fit tests. Furthermore, we used the Bayesian approach to obtain the predictive survival inferences from the best-fit data based on the exponentiated Weibull model. Results: We identified the highest number of black non-Hispanic female breast cancer patients in Michigan and the lowest in Hawaii. The mean (SD), of age at diagnosis (years) was 58.3 (14.43). The mean (SD), of survival time (months) for black non-Hispanic females was 66.8 (30.20). Non-Hispanic blacks had a significantly increased risk of death compared to Black Hispanics (Hazard ratio: 1.96, 95%CI: 1.51-2.54). Compared to other statistical probability models, we found that the exponentiated Weibull model better fits for the survival times. By making use of the Bayesian method predictive inferences for future survival times were obtained. Conclusions: These findings will be of great significance in determining appropriate treatment plans and health-care cost allocation. Furthermore, the same approach should contribute to build future predictive models for any health related diseases.