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쐐기형 레일클램프의 초기압착력 발휘를 위한 중추무게에 관한 연구
한근조,한동섭 東亞大學校 2005 東亞論叢 Vol.42 No.-
The wedge type rail clamp has the operating mechanism: First, the jaw pad clamps a rail with small clamping force. Next as the wind speed increases, the clamping force of the jaw pad is increased by the wedge. In order to design the wedge type rail clamp, we need to determine the proper wedge angle to minimize the sliding distance of a roller and the proper clamping angle of a locker to generate the initial clamping force of a jaw pad. The researches for the proper wedge angle have conducted, and in this study we conducted the investigation to determine the proper clamping angle of a locker in the rail clamp with wedge angle of 10˚. Because the initial clamping force of the jaw pad was determined by the clamping angle of the locker, in order to carry out the clamping force of a jaw pad, we measured the locking force applied to a locker with respect to the clamping angle of a locker, such as 3°, 4°, 5°, 6° using a pressure gauge, and compared the results with the FEA results.
한근조,안태원,이성욱,한동섭 동아대학교 생산기술연구소 2006 生産技術硏究所硏究論文集 Vol.11 No.-
Because strong wind is one of the few forces that, although considered in container crane design, still cause significant damage, a container crane was tested to investigate wind load characteristic in uniform flows. So, this study measured an external point pressure at the each members of a container crane according to a wind direction and a shape of members in a wind-tunnel test. The result of this test was compared to those of computation fluid dynamics using a CFX 10. The scale of a container crane model for wind tunnel test applied similarity scales to consider the size of the wind tunnel test section and the boundary condition for CFD is like wind tunnel test.
쐐기형 레일클램프에서 조의 회전을 방지하기 위한 적정 쐐기각에 대한 연구
한동섭,한근조,심재준,이성욱 東亞大學校 大學院 2005 大學院論文集 Vol.30 No.-
Recently many countries have concentrated their effort on the port automation, in order to be the Hub-port, that the ULCS (Ultra Large Container Ship) could come alongside the quay in their region. As the magnitude of the container ship increases, that of the container crane (used in quay) increases from 50ton-class to 61ton-class more and more. The wind velocity criteria to design the structures used in the port was upgraded from 30m/s to 40m/s due to change of the weather condition. When we design wedge type rail clamp which can protect container crane from sudden wind blast with constant clamping force regardless of the operating period, it is important to determine the angle of wedge within proper range. In this study we designed the wedge angle of the wedge-type rail clamp using the FEA.
Anti-Biofilm Effects of Torilis japonica Ethanol Extracts against Staphylococcus aureus
( Geun-seop Kim ),( Chae-rin Park ),( Ji-eun Kim ),( Hong-kook Kim ),( Byeong-soo Kim ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.2
The spread of antibiotic-resistant strains of Staphylococcus aureus, a gram-positive opportunistic pathogen, has increased due to the frequent use of antibiotics. Inhibition of the quorum-sensing systems of biofilm-producing strains using plant extracts represents an efficient approach for controlling infections. Torilis japonica is a medicinal herb showing various bioactivities; however, no studies have reported the anti-biofilm effects of T. japonica extracts against drug-resistant S. aureus. In this study, we evaluated the inhibitory effects of T. japonica ethanol extract (TJE) on biofilm production in methicillin-sensitive S. aureus (MSSA) KCTC 1927, methicillin-resistant S. aureus (MRSA) KCCM 40510, and MRSA KCCM 40511. Biofilm assays showed that TJE could inhibit biofilm formation in all strains. Furthermore, the hemolysis of sheep blood was found to be reduced when the strains were treated with TJE. The mRNA expression of agrA, sarA, icaA, hla, and RNAIII was evaluated using reverse transcription-polymerase chain reaction to determine the effect of TJE on the regulation of genes encoding quorum sensing-related virulence factors in MSSA and MRSA. The expression of hla reduced in a concentration-dependent manner upon treatment with TJE. Moreover, the expression levels of other genes were significantly reduced compared to those in the control group. In conclusion, TJE can suppress biofilm formation and virulence factor-related gene expression in MSSA and MRSA strains. The extract may therefore be used to develop treatments for infections caused by antibiotic-resistant S. aureus.
전통 밀누룩에서 분리한 유산균의 특성 및 마우스 대식세포에서의 Nitric Oxide 생성에 미치는 영향
김근섭(Geun-Seop Kim),최영호(Yeong-Ho Choe),김홍국(Hong-Kook Kim),김병수(Byeong-Soo Kim) 한국생물공학회 2018 KSBB Journal Vol.33 No.3
The purpose of this study is to assess of efficacy about lactic acid bacteria isolated form traditional wheat Nuruk. 6 strains was isolated from traditional Nuruk. The Strains identified by 16s rRNA gene sequencing are Lactobacillus Curvatus (named SA-13, YS-2), Pediococcus Acidilactici (named SS-5, YS-3) and Pediococcus Pentosaceus (named SA-2, YS-1). P. acidilactici SS-5 has Acid and bile tolerance. The bacteria free - supernatant of P. acidilactici SS-5 showed antibacterial activity in gram negative bacteria (Escherichia Coli, Salmonella Typhimurium) and gram positive bacteria (Staphylococcus Aureus, Listeria Monocytogenes) in paper disk diffusion assay. The bacteria free - supernatant of P. acidilactici SS-5 showed effect in all of used pathogenic bacteria at concentration of 11.25 ㎎/mL. All strains isolated from traditional Nuruk showed more than 60 percents on the antioxidant activity. Raw 264.7 cell line was used as a macrophage model to assess the effects on cytotoxic test and the production of nitric oxide. The nitric oxide productions of the viable bacteria and heat-inactivated bacteria of the P. acidilactici SS-5 were superior to other strains isolated Nuruk.