Detection of Plasmodium vivax by Nested PCR and Real-Time PCR

Genc,,Ahmet,Eroglu,,Fadime,Koltas,,Ismail,Soner The Korean Society for Parasitology 2010 The Korean Journal of Parasitology Vol.48 No.2

Malaria is endemic in the Cukurova region while it is sporadic in other regions of Turkey. Therefore, the laboratory and clinical diagnosis of malaria is important for the treatment of malaria. In this study, 92 blood samples that were taken from the suspected malaria patients for routine diagnosis in a period of 10 years between 1999 and 2009 were analyzed. All of these blood samples were examined by microscopic examinations using Giemsa-stained thick blood films, nested PCR, and real-time PCR. The sensitivity-specificity and positive-negative predictive values for these diagnostic tests were then calculated. It was found that the positive predictive values of microscopic examination of thick blood films, nested PCR, and real-time PCR were 47.8%, 56.5%, and 60.9% for malaria, respectively. The real-time PCR was found to have a specificity of 75% and sensitivity of 100%, while specificity and sensitivity of nested PCR was found 81.2% and 97.7% according to the microscopic examination of thick blood films, respectively.

Determination of Knowledge of Turkish Midwifery Students about Human Papilloma Virus Infection and its Vaccines

Genc,,Rabia,Ekti,Sarican,,Emine,Serap,Turgay,,Ayse,San,Icke,,Sibel,Sari,,Dilek,Saydam,,Birsen,Karaca Asian Pacific Organization for Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Human papilloma virus (HPV) is one of the most common sexually transmitted agents and its infection is the most established cause of cervical cancer. Midwives play a key position in the implementation of cervical cancer. This descriptive study aimed to determine the level of knowledge concerning HPV and HPV vaccination among 268 midwifery students. Data were collected between November 15 and 30, 2011, through a self-reported questionnaire. The mean age of participants was $20.75{\pm}1.60$. Among all students, 44.4% had heard of HPV, while 40.4% had heard of HPV vaccinatiob. The relationship between the midwifery student knowledge on HPV and HPV vaccine and their current educational year was significant (p=0.001). In conclusion midwifery students have moderate level of knowledge about HPV and its vaccine and relevant information should be included in their teaching curriculum.

Preparation of Prolonged Release Clarithromycin Microparticles for Oral Use and Their In Vitro Evaluation

Genc,,Lutfi,Demirel,,Muzeyyen,Yazan,,Yasemin The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.10

Prolonged release micro particles of clarithromycin (CL) were prepared using Eudragit RL 100 and RS 100 by spray-drying and casting-drying techniques. For the characterization of those microparticles, preparation yield, particle size distribution, X-ray diffraction, thermal behavior, active agent content and in vitro dissolution from the microparticles were performed. HPLC was used for the assay of clarithromycin and the assay method was validated. All the formulations obtained showed prolonged release when compared to pure clarithromycin. Microparticles prepared by spray-drying method had a slower release compared to those of casting drying method. Spray-drying method seems to be a more suitable method to prepare microparticles for prolongation in release.

Studies on the Different Reaction Pathways between 3-Acetyl-5-benzoyl-6-methyl-2-phenyl-4H-pyran-4-one and Alkylamines

Genc,,Hasan,Tan,,Meltem,Gumus,,Selcuk,Menges,,Nurettin,Bildirici,,Ishak,Sener,,Ahmet Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.9

3-Acetyl-5-benzoyl-6-methyl-2-phenyl-4H-pyran-4-one has been subjected to condensation with a series of primary amines (ethylamine - octylamine) to clarify the proposed mechanism in our previous study. The reactions of the shorter amines of the series (ethylamine - butylamine) yielded unsymmetric pyridinone products, whereas the other amines (pentylamine - octylamine) yielded symmetrical pyridinones. The starting material and the products as well as the intermediates have been subjected to theoretical analysis by quantum chemical calculations at B3LYP/6-31G(d,p) level, which provided supporting data for the experimental findings.

Femoral Neuropathy in a Patient with Rheumatoid Arthritis

Hakan,Genc,Ozlem,Balaban,Aynur,Karagoz,Hatice,Rana,Erdem 연세대학교의과대학 2007 Yonsei medical journal Vol.48 No.5

Femoral mononeuropathy (FMN) as an extraarticular finding of rheumatoid arthritis (RA) is a phenomenon which has not been reported previously. We report a 53-year-old female patient with RA, presenting FMN findings during the course of the disease. On examination, right quadriceps and iliopsoas muscles showed grade 3 weakness on the Medical Research Council (MRC) scale. Sensory examination revealed sensory loss in the right medial leg and thigh. Patellar tendon reflex was absent in the right side. A diagnosis of a partial right femoral neuropathy was confirmed using nerve conduction study and electromyography. The probable mechanism of FMN was thought to be vasculitis.

Detection of Plasmodium vivax by Nested PCR and Real-Time PCR

Ahmet,Genc,Fadime,Eroglu,Ismail,Soner,Koltas 대한기생충학열대의학회 2010 The Korean Journal of Parasitology Vol.48 No.2

Malaria is endemic in the Cukurova region while it is sporadic in other regions of Turkey. Therefore, the laboratory and clinical diagnosis of malaria is important for the treatment of malaria. In this study, 92 blood samples that were taken from the suspected malaria patients for routine diagnosis in a period of 10 years between 1999 and 2009 were analyzed. All of these blood samples were examined by microscopic examinations using Giemsa-stained thick blood films, nested PCR, and real-time PCR. The sensitivity-specificity and positive-negative predictive values for these diagnostic tests were then calculated. It was found that the positive predictive values of microscopic examination of thick blood films, nested PCR, and real-time PCR were 47.8%, 56.5%, and 60.9% for malaria, respectively. The real-time PCR was found to have a specificity of 75% and sensitivity of 100%, while specificity and sensitivity of nested PCR was found 81.2% and 97.7% according to the microscopic examination of thick blood films, respectively.

Case Reports : Splenic infarction as a complication of celiac artery thromboembolism: an unusual cause of abdominal pain

( Volkan Genc ), ( Omer Arda Cetinkaya ), ( Ilgaz Kayilioglu ), ( Ahmet Serdar Karaca ), ( Gokhan Cipe ), ( Ali Ekrem Unal ) 대한내과학회 2011 대한내과학회지 Vol.81 No.5

Splenic infarction is a relatively uncommon diagnosis and this clinical presentation can mimic other causes of acute abdominal pain. Cardiologic and hematologic disorders are common reasons for this entity. There have been a few series and single case reports of splenic infarction published in peer-reviewed medical journals. We report a 53-year-old patient who had splenic infarction caused by celiac artery thromboembolism. The importance of this case, without any etiological predisposing factors, is that this kind of clinical situation should be considered in the differential diagnosis of abdominal pain.

Number Recognition of Parts Book Schematics using Convolutional Recurrent Neural Network

Erdal,Genc,Hee,Ran,Shin,Jang,Sik,Park 제어로봇시스템학회 2018 제어로봇시스템학회 국제학술대회 논문집 Vol.2018 No.10

Experimental study on the rheology of anisotropic, flocculated and low volume fraction colloids

Burcu,Genc,Ozel,Aslihan,Orum,Mehmet,Yildiz,Yusuf,Z.,Menceloglu 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.1

In this work, we have investigated rheological behavior of colloids with a low particle volume fraction, andanisotropic and flocculated microstructures through measuring their viscosity and electrical resistance undervarying shear rates together with utilizing several relevant characterization methods (i.e., Dynamic LightScattering, Transmission Electron Microscopy, Atomic Force Microscopy, and Capacitance and Electricalresistance measurements). The colloids are formed through the suspension of hydrophilic/phobic fumed silicaparticle with attractive/repulsive interaction in polyethylene glycol and/or ethylene oxide-propyleneoxide copolymer. It is observed that studied suspensions display shear thickening/thinning flow behaviordepending on their microstructure (the spatial distribution and arrangements of particles in continuousmedia) and associated changes in cluster sizes, which are controlled by the break down of densified clusters(due to the shear induced mechanical and hydrodynamical forces) and the interaction forces among particleparticleand particles-polymers (owing to physicochemical effects). The detailed evaluation of the experimentalresults indicates that the shear thickening phenomena in low volume fraction, anisotropic and flocculatedsystems can be mainly attributed to the increase in the effective volume fraction of particles due toboth hydrodynamic and physicochemical forces.

The Effect of Stinging Nettle (Urtica dioica) Seed Oil on Experimental Colitis in Rats

Zeynep,Genc,Aysen,Yarat,Tugba,Tunali-Akbay,Goksel,Sener,Sule,Cetinel,Rabia,Pisiriciler,Esin,Caliskan-Ak,Ayhan,Altıntas,Betul,Demirci 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.12

This study investigated the effect of Urtica dioica, known as stinging nettle, seed oil (UDO) treatment on colonic tissue and blood parameters of trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. Experimental colitis was induced with 1mL of TNBS in 40% ethanol by intracolonic administration with a 8-cm-long cannula with rats under ether anesthesia, assigned to a colitis group and a colitis +UDO group. Rats in the control group were given saline at the same volume by intracolonic administration. UDO (2.5mL/kg) was given to the colitis +UDO group by oral administration throughout a 3-day interval, 5 minutes later than colitis induction. Saline (2.5 mL/kg) was given to the control and colitis groups at the same volume by oral administration. At the end of the experiment macroscopic lesions were scored, and the degree of oxidant damage was evaluated by colonic total protein, sialic acid, malondialdehyde (MDA), and glutathione levels, collagen content, tissue factor activity, and superoxide dismutase and myeloperoxidase activities. Colonic tissues were also examined by histological and cytological analysis. Pro-inflammatory cytokines (tumor necrosis factor-a, interleukin-1b, and interleukin-6), lactate dehydrogenase activity, and triglyceride and cholesterol levels were analyzed in blood samples. We found that UDO decreased levels of pro-inflammatory cytokines, lactate dehydrogenase, triglyceride, and cholesterol, which were increased in colitis. UDO administration ameliorated the TNBS-induced disturbances in colonic tissue except for MDA. In conclusion, UDO, through its anti-inflammatory and antioxidant actions, merits consideration as a potential agent in ameliorating colonic inflammation.