Subgingival pathogens in chronic periodontitis patients affected by type 2 diabetes mellitus: a retrospective case-control study

Montevecchi, Marco,Valeriani, Leoluca,Gatto, Maria Rosaria,D'Alessandro, Giovanni,Piana, Gabriela Korean Academy of Periodontology 2021 Journal of Periodontal & Implant Science Vol.51 No.-

Purpose: The aim of this study was to compare the prevalence and bacterial load of 6 main periodontal pathogens between pairs of periodontal patients with and without type 2 diabetes mellitus. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans genotypes were also investigated. Methods: Twenty patients affected by chronic periodontitis and type 2 diabetes were retrospectively selected and matched to 20 patients without diabetes on the basis of the degree and severity of periodontal disease. Microbiological data of subgingival biofilms were analysed and compared for the examined pathogens: A. actinomycetemcomitans, P. gingivalis, Prevotella intermedia, Treponema denticola, Fusobacterium nucleatum, and Tannerella forsythia. Results: The pairs were balanced in terms of demographic and clinical parameters, except for bleeding on probing and suppuration. In the microbiological test sites (4 for each patient), the mean probing pocket depth was 6.34±1.63 mm in patients with diabetes and 6.41±1.78 mm in patients without diabetes. No significant difference between pairs in the prevalence of P. gingivalis or the distribution of its genotypes was recorded. Patients with diabetes had a significantly greater amount of total bacterial load, P. gingivalis, T. denticola, T. forsythia, and F. nucleatum (P<0.05). Moreover, patients with diabetes had a higher number of sites with a greater cell count than patients without diabetes. When compared to the total bacterial load, only T. forsythia maintained its relative load in patients with diabetes (P=0.001). Conclusions: This retrospective matched study supports the hypothesis that microbiological differences exist among periodontal patients with and without diabetes mellitus.

Self-cleaning measurements on tiles manufactured with micro-sized photoactive TiO₂

Bianchi, C.L.,Gatto, S.,Nucci, S.,Cerrato, G.,Capucci, V. Techno-Press 2013 Advances in materials research Vol.2 No.1

Heterogeneous photocatalysis is a rapidly developing field in environmental engineering. It has a great potential to cope with the increasing pollution in the air. The addition of a photocatalyst to ordinary building materials such as tiles, concrete, paints, creates environmental friendly materials by which air pollution or pollution of the surface itself can be controlled and diminished. This work reports the results of the laboratory research, especially carried out towards air purifying action and self-cleaning measurements. In particular the research was focused on the study of the photocatalytic behavior of industrially prepared tiles produced starting from commercial micro-sized $TiO_2$. Air purification action has been investigated through NOx degradation tests. On the contrary, the degradation of pollution at the surface, also called as self-cleaning action, is verified by the degradation of two different organic dyes: Rhodamine B (red color) and Metanil yellow (yellow).

A comparison on static and fatigue behaviour between traditional and SLM AISI 316L

Danilo D’Andrea,Andrea Gatto,Eugenio Guglielmino,Giacomo Risitano,Dario Santonocito 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.3

Additive manufacturing (AM) is a novel production process, that allows the creation of lightweight and customizable devices. Such capacity requires a high grade of reliability under the action of service loads, especially under fatigue loads. One of the high-performance steels adopted for selective laser melting (SLM) is the AISI 316L. However, SLM is severally affected by laser beam power and scanning velocity. Mechanical properties must be investigated to provide reliability of final products, but this requires a huge amount of time. On the other hand, energy methods, such as the Risitano’s thermographic method and the novel static thermographic method, by performing stepwise fatigue tests and static tensile tests, can assess the material’s performance by monitoring the energy release. In this work, failure analysis has been performed, showing the differences between the traditional and AM manufacturing process, giving an insight to understand and improve the quality of the AM production process.

The influence of root surface distance to alveolar bone and periodontal ligament on periodontal wound healing

Montevecchi, Marco,Parrilli, Annapaola,Fini, Milena,Gatto, Maria Rosaria,Muttini, Aurelio,Checchi, Luigi Korean Academy of Periodontology 2016 Journal of Periodontal & Implant Science Vol.46 No.5

Purpose: The purpose of this animal study was to perform a 3-dimensional micro-computed tomography (micro-CT) analysis in order to investigate the influence of root surface distance to the alveolar bone and the periodontal ligament on periodontal wound healing after a guided tissue regeneration (GTR) procedure. Methods: Three adult Sus scrofa domesticus specimens were used. The study sample included 6 teeth, corresponding to 2 third mandibular incisors from each animal. After coronectomy, a circumferential bone defect was created in each tooth by means of calibrated piezoelectric inserts. The experimental defects had depths of 3 mm, 5 mm, 7 mm, 9 mm, and 11 mm, with a constant width of 2 mm. One tooth with no defect was used as a control. The defects were covered with a bioresorbable membrane and protected with a flap. After 6 months, the animals were euthanised and tissue blocks were harvested and preserved for micro-CT analysis. Results: New alveolar bone was consistently present in all experimental defects. Signs of root resorption were observed in all samples, with the extent of resorption directly correlated to the vertical extent of the defect; the medial third of the root was the most commonly affected area. Signs of ankylosis were recorded in the defects that were 3 mm and 7 mm in depth. Density and other indicators of bone quality decreased with increasing defect depth. Conclusions: After a GTR procedure, the periodontal ligament and the alveolar bone appeared to compete in periodontal wound healing. Moreover, the observed decrease in bone quality indicators suggests that intrabony defects beyond a critical size cannot be regenerated. This finding may be relevant for the clinical application of periodontal regeneration, since it implies that GTR has a dimensional limit.

Subgingival pathogens in chronic periodontitis patients affected by type 2 diabetes mellitus: a retrospective case-control study

Marco Montevecchi,Leoluca Valeriani,Maria Rosaria Gatto,Giovanni D'Alessandro,Gabriela Piana 대한치주과학회 2021 Journal of Periodontal & Implant Science Vol.51 No.6

Purpose: The aim of this study was to compare the prevalence and bacterial load of 6 main periodontal pathogens between pairs of periodontal patients with and without type 2 diabetes mellitus. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans genotypes were also investigated. Methods: Twenty patients affected by chronic periodontitis and type 2 diabetes were retrospectively selected and matched to 20 patients without diabetes on the basis of the degree and severity of periodontal disease. Microbiological data of subgingival biofilms were analysed and compared for the examined pathogens: A. actinomycetemcomitans, P. gingivalis, Prevotella intermedia, Treponema denticola, Fusobacterium nucleatum, and Tannerella forsythia. Results: The pairs were balanced in terms of demographic and clinical parameters, except for bleeding on probing and suppuration. In the microbiological test sites (4 for each patient), the mean probing pocket depth was 6.34±1.63 mm in patients with diabetes and 6.41±1.78 mm in patients without diabetes. No significant difference between pairs in the prevalence of P. gingivalis or the distribution of its genotypes was recorded. Patients with diabetes had a significantly greater amount of total bacterial load, P. gingivalis, T. denticola, T. forsythia, and F. nucleatum (P<0.05). Moreover, patients with diabetes had a higher number of sites with a greater cell count than patients without diabetes. When compared to the total bacterial load, only T. forsythia maintained its relative load in patients with diabetes (P=0.001). Conclusions: This retrospective matched study supports the hypothesis that microbiological differences exist among periodontal patients with and without diabetes mellitus. Trial Registration: ClinicalTrials.gov Identifier: NCT03786133

The influence of root surface distance to alveolar bone and periodontal ligament on periodontal wound healing

Marco Montevecchi,Annapaola Parrilli,Milena Fini,Maria Rosaria Gatto,Aurelio Muttini,Luigi Checchi 대한치주과학회 2016 Journal of Periodontal & Implant Science Vol.46 No.5

Purpose: The purpose of this animal study was to perform a 3-dimensional micro-computed tomography (micro-CT) analysis in order to investigate the influence of root surface distance to the alveolar bone and the periodontal ligament on periodontal wound healing after a guided tissue regeneration (GTR) procedure. Methods: Three adult Sus scrofa domesticus specimens were used. The study sample included 6 teeth, corresponding to 2 third mandibular incisors from each animal. After coronectomy, a circumferential bone defect was created in each tooth by means of calibrated piezoelectric inserts. The experimental defects had depths of 3 mm, 5 mm, 7 mm, 9 mm, and 11 mm, with a constant width of 2 mm. One tooth with no defect was used as a control. The defects were covered with a bioresorbable membrane and protected with a flap. After 6 months, the animals were euthanised and tissue blocks were harvested and preserved for micro-CT analysis. Results: New alveolar bone was consistently present in all experimental defects. Signs of root resorption were observed in all samples, with the extent of resorption directly correlated to the vertical extent of the defect; the medial third of the root was the most commonly affected area. Signs of ankylosis were recorded in the defects that were 3 mm and 7 mm in depth. Density and other indicators of bone quality decreased with increasing defect depth. Conclusions: After a GTR procedure, the periodontal ligament and the alveolar bone appeared to compete in periodontal wound healing. Moreover, the observed decrease in bone quality indicators suggests that intrabony defects beyond a critical size cannot be regenerated. This finding may be relevant for the clinical application of periodontal regeneration, since it implies that GTR has a dimensional limit.

Effects of ultrasonic instrumentation with different scaler-tip angulations on the shear bond strength and bond failure mode of metallic orthodontic brackets

Giulio Alessandri Bonetti,Serena Incerti Parenti,Daniela Rita Ippolito,Maria Rosaria Gatto,Luigi Checchi 대한치과교정학회 2014 대한치과교정학회지 Vol.44 No.1

Objective: To evaluate the effects of ultrasonic instrumentation with different scaler-tip angulations on the shear bond strength (SBS) and bond failure mode of metallic orthodontic brackets. Methods: Adhesive pre-coated metallic brackets were bonded to 72 extracted human premolars embedded in autopolymerizing acrylic resin. The teeth were randomly divided into 3 groups (n = 24 each) to undergo no treatment (control group) or ultrasonic instrumentation with a scaler-tip angulation of 45˚ (45˚-angulation group) or 0˚ (0˚-angulation group). SBS was tested in a universal testing machine, and adhesive remnant index (ARI) scores were recorded. The Kruskal-Wallis test and Mann-Whitney U-test were used for statistical analysis. Results: The control group had a significantly higher mean SBS value than the treated groups, which showed no significant differences in their mean SBS values. The ARI scores were not significantly different among the groups. Conclusions: Ultrasonic instrumentation around the bracket base reduces the SBS of metallic orthodontic brackets, emphasizing the need for caution during professional oral hygiene procedures in orthodontic patients. The scaler-tip angulation does not influence the SBS reduction and bond failure mode of such brackets.

Internal Carotid Artery Dissection in Brazilian Jiu-Jitsu

Zeferino Demartini Jr,Maxweyd Rodrigues Freire,Roberto Oliver Lages,Alexandre Novicki Francisco,Felipe Nanni,Luana A. Maranha Gatto,Gelson Luis Koppe 대한뇌혈관외과학회 2017 Journal of Cerebrovascular and Endovascular Neuros Vol.19 No.2

Carotid artery dissection is a significant cause of stroke in young patients. It may be asymptomatic and go undiagnosed, or minimal transient manifestations may follow, commanding a higher index of suspicion than ordinarily exists to avoid misdiagnosis. Reported herein is a 27-year-old man who suffered extracranial internal carotid artery dissection while practicing a Brazilian Jiu-Jitsu submission maneuver. The patient's condition suddenly deteriorated one week later due to distal embolization and stroke. Despite endovascular treatment, with stenting of the cervical carotid artery, neurologic deficits remained. Of note, the objective in martial arts, which is to kill or incapacitate, has yet to be fully tempered in transitioning to sport. Brazilian Jiu-jitsu, a relatively new and fast-growing form of martial art, places emphasis on submission maneuvers. Related injuries are not common knowledge and are poorly described in the literature. This account is intended to shed light on the risk of this discipline. Through education and improved supervision, vascular injuries of this nature and the potentially lethal or disabling consequences may thus be prevented in young athletes.

Juvenile and Mature Pinewoods Treated by in situ Polymerization with Poly(vinyl acetate)

Andrey Acosta,Ezequiel GALLIO,Paula Zanatta,Henrique Schulz,Rafael de Avila Delucis,Darci Gatto 한국섬유공학회 2021 Fibers and polymers Vol.22 No.3

This study aims to produce and characterize wood-based composites (WPC) by in situ polymerization withpoly(vinyl acetate) into juvenile and mature woods from a pine specie. Vacuum-pressure method was used to impregnate thewood with a vinyl acetate monomer and benzoyl peroxide (1.5 wt%) was used as initiator. The studied WPC werecharacterized for treatment parameters (weight percentage gain and specific gravity), as well as chemical (by infraredspectroscopy), thermal (by thermogravimetric analysis) and mechanical (by flexure and hardness tests) features. The in situpolymerization yielded improved mechanical properties and increased thermal stabilities, which were attributed to theimpregnated poly(vinyl acetate) (especially on the wood surface) as suggested by treatment parameters and infrared spectra. The presented wood treatment may be a key strategy to improve pinewood properties, especially from juvenile wood, whichmay overcome the properties of the pristine mature wood.

Chemical, Mechanical, Thermal, and Colorimetric Features of the Thermally Treated Eucalyptus grandis Wood Planted in Brazil

( Henrique Römer Schulz ),( Andrey Pereira Acosta ),( Kelvin Techera Barbosa ),( Mario Antonio Pinto Da Silva Junior ),( Ezequiel Gallio ),( Rafael De Ávila Delucis ),( Darci Alberto Gatto ) 한국목재공학회 2021 목재공학 Vol.49 No.3

This article aimed at thermally treating and charactering the Eucalyptus grandis wood under three different temperatures. For this, pristine eucalypt samples were treated by heating in a laboratory oven at 160 °C, 200 °C and 240 °C, always for 2 h. Treatment parameters (based on weight percentage loss and specific gravity), as well as mechanical (by hardness tests), chemical (by infrared spectroscopy), thermal (by thermogravimetry), and colorimetric (by CIELab method) features were evaluated. Compared to the pristine ones, the treated woods have there was a drop in apparent density at 12 % and consecutively greater thermal stability which is probably related to a previous partial degradation of some major amorphous components (namely cellulose, hemicellulose and lignin), as suggested by the treatment parameters and infrared spectra. Besides of that, the higher the temperature treatment, the higher the loss in surface hardness and the higher the colour darkening.