Anti-diabetic Potential of Silver Nanoparticles Synthesized with Argyreia nervosa Leaf Extract High Synergistic Antibacterial Activity with Standard Antibiotics Against Foodborne Bacteria

Saratale, Ganesh Dattatraya,Saratale, Rijuta Ganesh,Benelli, Giovanni,Kumar, Gopalakrishnan,Pugazhendhi, Arivalagan,Kim, Dong-Su,Shin, Han-Seung Springer-Verlag 2017 Journal of cluster science Vol.28 No.3

<P>The current investigation highlighted a novel cost-effective green synthesis of silver nanoparticles (AgNPs) using Argyreia nervosa leaves extract (ANE) as a potential reducing and capping agent. Surface plasmon resonance confirmed the formation of AgNPs with maximum absorbance at lambda (max) = 435 nm. FTIR revealed the involvement of biological macromolecules of ANE in the synthesis and stabilization of AgNPs. HRTEM images showed that the size of the spherical AgNPs ranged between 5 and 40 nm with average particle size of about 15 nm. The ANE-AgNPs showed inhibition activity against carbohydrate digestive enzymes alpha-amylase and alpha-glucosidase, with EC50 of 55.5 and 51.7 A mu g/mL, respectively, indicating its antidiabetic potential. The in vitro antioxidant activity of ANE-AgNPs was evaluated in terms of ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radicals scavenging assays with IC50 value of 44.3 and 55.9 A mu g/mL, respectively. The AgNPs displayed strong antibacterial activity against foodborne bacteria with zone of inhibition 16.0 and 12.5 mm for Escherichia coli and Staphylococcus aureus, respectively, and also exhibited strong synergistic antibacterial activity together with standard antibiotics. The biological activity in terms of antioxidant, antidiabetic and antibacterial potential could be useful in various bio-applications such as cosmetics, food, and biomedical industry.</P>

Screening and optimization of pretreatments in the preparation of sugarcane bagasse feedstock for biohydrogen production and process optimization

Saratale, Ganesh Dattatray,Saratale, Rijuta Ganesh,Kim, Sang Hyoun,Kumar, Gopalakrishnan Elsevier 2018 International journal of hydrogen energy Vol.43 No.25

<P><B>Abstract</B></P> <P>This work evaluated the effects of individual alkaline, sodium carbonate (Na<SUB>2</SUB>CO<SUB>3</SUB> denoted as; NaC), sodium sulfide (Na<SUB>2</SUB>SO<SUB>3</SUB> denoted as; NaS) and combination of NaC + NaS pretreatment for the saccharification of sugarcane bagasse (SCB). The effects of different pretreatments on chemical composition and structural complexity of SCB in relation with its saccharification were investigated. For enzymatic hydrolysis of pretreated SCB we have utilized the produced crude enzymes by <I>Streptomyces</I> sp. MDS to make the process more cost effective. A enzyme dose of 30 filter paperase (FPU) produced a maximum reducing sugar (RS) 592 mg/g with 80.2% hydrolysis yield from NaC + NaS pretreated SCB under optimized conditions. The resulted enzymatic hydrolysates of each pretreated SCB were applied for hydrogen production using <I>Clostridium beijerinckii</I> KCTC1785. NaC + NaS pretreated SCB hydrolysates exhibited maximum H<SUB>2</SUB> production relative to other pretreatment methods. Effects of temperature, initial pH of culture media and increasing NaC + NaS pretreated SCB enzymatic hydrolysates concentration (2.5–15 g/L) on bioH<SUB>2</SUB> production were investigated. Under the optimized conditions, the cumulative H<SUB>2</SUB> production, H<SUB>2</SUB> production rate, and H<SUB>2</SUB> yield were 1485 mL/L, 61.87 mL/L/h and 1.24 mmol H<SUB>2</SUB>/mol of RS (0.733 mmol H<SUB>2</SUB>/g of SCB), respectively. The efficient conversion of the SCB hydrolysate to H<SUB>2</SUB> without detoxification proves the viability of process for cost-effective hydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pretreatments of alkaline, sodium carbonate, sodium sulfide and combination demonstrated for sugarcane baggase. </LI> <LI> Several crucial factors towards optimization were carried out. </LI> <LI> Peak 59.2 g/L of reducing sugar (RS) with 80.2% hydrolysis yield from NaC + NaS pretreated SCB. </LI> <LI> Significant hydrogen production and process optimization using SCB hydrolysates studied in detail. </LI> </UL> </P>

Electrochemical Oxidation of Phenol for Wastewater Treatment Using Ti/PbO2 Electrode

Saratale, Rijuta Ganesh,Hwang, Kyoung-Jin,Song, Ji-Young,Saratale, Ganesh Dattatray,Kim, Dong-Su American Society of Civil Engineers 2016 Journal of environmental engineering Vol.142 No.2

<P>The electrochemical oxidation of phenol was studied using a Ti/PbO2 electrode prepared by the electrodeposition method with PbO2 coated on Ti. The structural and morphological activity of Ti/PbO2 was analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD), and intermediates formed after degradation of phenol were quantitatively assessed by high-pressure liquid chromatography (HPLC). Optimization of various parameters such as current density, initial phenol concentration, initial solution pH, and different temperature and dose of Fe2+ on electrochemical degradation of phenol using Ti/PbO2 were investigated. Complete removal of phenol (250mgL-1) was observed at 50 degrees C, potential difference (5V), and at pH 2. Experimental results showed that the phenol removal rate increased with increasing current intensity along with significant reduction in total organic carbon (TOC). Fundamental kinetic data obtained for the degradation of phenol by Ti/PbO2 was found to follow in accordance with the zero-order kinetics with respect to the phenol concentration. This paper is expected to be useful for the development of electrochemical process using Ti/PbO2 for the degradation of phenol containing wastewater. (C) 2015 American Society of Civil Engineers.</P>

Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production

Saratale, Rijuta Ganesh,Shin, Han Seung,Ghodake, Gajanan S.,Kumar, Gopalakrishnan,Oh, Min Kyu,Saratale, Ganesh Dattatraya Elsevier 2018 Bioresource technology Vol.258 No.-

<P><B>Abstract</B></P> <P>This study focuses on development of calcium peroxide (CaO<SUB>2</SUB>) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO<SUB>4</SUB>) and cobalt chloride (COCl<SUB>2</SUB>) in CaO<SUB>2</SUB> pretreatment resulted in maximum delignification of KCP relative to individual CaO<SUB>2</SUB> pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain <I>Klebsiella pneumoniae</I> KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Developed CaO<SUB>2</SUB> pretreatment & optimized various operational parameters for KCP biomass. </LI> <LI> Addition of inorganic salts improved delignification, glucose yield and total sugar recovery. </LI> <LI> Maximum BDO production (10.42 g/L) was achieved using <I>Klebsiella pneumoniae</I> KMK05. </LI> <LI> This novel pretreatment strategy abstained loss of sugar components of biomass. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2P-135 Study of Non-linear PL Behavior of Chemically Treated Monolayer TMDs by Exciton Exciton Annihilation

( Ganesh Ghimire ),( Yongjun Lee ),( Seokjoon Yun ),서창원,( Hyun Kim ),( Roy Aad ),( Young Hee Lee ),김정용 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1

Monolayer transition metal dichalcogenide (1L-TMDs) provides a convenient platform to design light emission from 2 dimensionally confined excitonic systems. Many body effects arising from strong interactions among carriers have been of considerable interest in nanoscience. The exciton-exciton annihilation (EEA) process reduces the generated excitons density and affects the performances of the opto-electronic devices. We studied multiexciton dynamics in chemically treated TMDs like MoS₂, WS₂ and WSe₂ using non-linear photoluminescence (PL) spectroscopy. We also studied the many body correlations between excitons in pristine and chemically treated 1L-TMDs by excitation power dependence of PL and time resolved PL spectroscopy. We observed the decrease of the EEA rate after the chemical treatment.

Dye anchored ZnO nanoparticles: The positive and negative photoluminescence quenching effects

Ganesh, T.,Kim, Jong Hoon,Yoon, Seog Joon,Lee, Sangjin,Lee, Wonjoo,Mane, Rajaram S.,Han, Jin Wook,Han, Sung-Hwan American Institute of Physics 2009 JOURNAL OF APPLIED PHYSICS - Vol.106 No.8

Physics based basis function for vibration analysis of high speed rotating beams

Ganesh, R.,Ganguli, Ranjan Techno-Press 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.39 No.1

The natural frequencies of continuous systems depend on the governing partial differential equation and can be numerically estimated using the finite element method. The accuracy and convergence of the finite element method depends on the choice of basis functions. A basis function will generally perform better if it is closely linked to the problem physics. The stiffness matrix is the same for either static or dynamic loading, hence the basis function can be chosen such that it satisfies the static part of the governing differential equation. However, in the case of a rotating beam, an exact closed form solution for the static part of the governing differential equation is not known. In this paper, we try to find an approximate solution for the static part of the governing differential equation for an uniform rotating beam. The error resulting from the approximation is minimized to generate relations between the constants assumed in the solution. This new function is used as a basis function which gives rise to shape functions which depend on position of the element in the beam, material, geometric properties and rotational speed of the beam. The results of finite element analysis with the new basis functions are verified with published literature for uniform and tapered rotating beams under different boundary conditions. Numerical results clearly show the advantage of the current approach at high rotation speeds with a reduction of 10 to 33% in the degrees of freedom required for convergence of the first five modes to four decimal places for an uniform rotating cantilever beam.

Synthesis and characterizations of activated carbon from Wisteria sinensis seeds biomass for energy storage applications

Ganesh Prasad Awasthi,Deval Prasad Bhattarai,Bikendra Maharjan,김경석,박찬희,김철상 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.72 No.-

Herein, we proposed, for thefirst time, a novel activated carbon (AC) material synthesized from Wisteriasinensis (WS) seeds biomass. Characterizations of the material were carried out by Field-emissionscanning electron microscopy, Transmission electron microscopy, X-ray diffraction, Raman spectroscopy,Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy, and Brunauer-Emmett-Teller specific surface area analysis. As-synthesized AC was employed as an electrode material to evaluateits applicability in energy storage devices. Results showed enhanced capacitive performance in an acidicelectrolyte compared to the neutral electrolyte at similar current density. Thus, as-synthesized AC couldbe a suitable material for energy storage applications.

Michaelis-Menten Behaviour in the Oxidation of Benzaldehydes by Pyridinium Chlorochromate (Corey's Reagent)

Ganesh P. Panigrahi,Sasananda Padhy Korean Chemical Society 1992 Bulletin of the Korean Chemical Society Vol.13 No.5

The oxidation of benzaldehyde, 3,4-dimethoxy benzaldehyde, p-methoxy benzaldehyde, m-$NO_2$-benzaldehyde, and m-chlorobenzaldehyde by pyridinium chlorochromate (Corey's reagent) are reported. Michaelis-Menten behaviour is observed. The rate determining step appears to be the decomposition of a complex of benzaldehyde with PCC either through a loss of $H^+$ or $H^-$ ions.

An integrated microfluidic PCR system with immunomagnetic nanoparticles for the detection of bacterial pathogens

Ganesh, Irisappan,Tran, Buu Minh,Kim, Yonghee,Kim, Jaewon,Cheng, Hua,Lee, Nae Yoon,Park, Sungsu Springer-Verlag 2016 Biomedical microdevices Vol.18 No.6

<P>There is growing interest in rapid microbial preconcentration methods to lower the detection limit of bacterial pathogens of low abundance in samples. Here, we report an integrated microfluidic PCR system that enables bacterial cells of interest in samples to be concentrated prior to PCR. It consists of two major compartments: a preconcentration chamber for the immunomagnetic separation of bacterial cells, and a PCR chamber for the DNA amplification of the concentrated cells. We demonstrate the feasibility of the system for the detection of microbial pathogens by preconcentrating the human pathogen Escherichia coli O157: H7, and also amplifying its DNA. The detection limit of E. coli O157: H7 in the PCR system is 1 x 103 CFU (colony forming unit)/mL. Onchip processing steps, including preconcentration and PCR steps, take less than two hours. Our system can serve as a rapid, specific, and quantitative platform for the detection of microbial pathogens in samples of large volume.</P>