Violence Against Women : The implementation of Law Against Domestic Violence in Timor-Leste

Francelina GUTERRES 이화여자대학교 아시아여성학센터 2012 이화여자대학교 아시아여성학센터 학술대회자료집 Vol.2012 No.1

Genotoxic and Antigenotoxic Potential of Momordica charantia Linn (Cucurbitaceae) in the Wing Spot Test of Drosophila melanogaster

Zaira Rosa Guterres,Thalita Alves Zanetti,Tiago Felipe Sennes-Lopes,Ana Francisca Gomes da Silva 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.10

Momordica charantia, popularly known as bitter melon, is a plant widely used in ethnobotanical medicine. It has antibacterial, antifungal, anthelmintic, antidiabetic, antiviral, and antimalarial activities, among others. The goal of this study was to evaluate the genotoxic and/or antigenotoxic activity of the aqueous extracts obtained from the aerial parts and fruit of this plant by means of the Drosophila melanogaster wing spot test. Third-stage larvae that obtained standard (ST) cross and high bioactivation (HB) cross were treated with aqueous extracts of the aerial parts (IQA) and fruit (IQF) of M. charantia, following two protocols (genotoxicity and antigenotoxicity). The aqueous extracts are not genotoxic in lower concentrations. The frequencies of mutant spots observed in the descendants of the ST and HB crosses treated with doxorubicin (DXR) alone were 8.65 and 9.25, respectively, whereas in those cotreated with IQA and DXR, the frequencies ranged from 15.90 to 29 in the ST cross and from 15.05 to 24.78 in the HB cross. In cotreatment with IQF, the frequencies ranged from 30.10 to 30.65 in the ST cross and from 13.60 to 14.50 in the HB cross, whereas the frequencies obtained with DXR were 32.50 in the ST cross and 26.00 in the HB cross. In conclusion, the IQA has a synergistic effect, enhancing the genotoxicity of DXR in the ST cross and the HB cross, whereas the IQF has antigenotoxic effects in the HB cross.

Preliminary Studies on the Antinociceptive Activity of Vaccinium ashei Berry in Experimental Animal Models

Maria Rosana Ramirez,Leandra Guterres,Odila E. Dickel,Micheli R. de Castro,Amelia T. Henriques,Ma´rcia M. de Souza,Daniela Martı´ Barros 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.2

The aim of this study was to carry out pharmacological screening in order to evaluate the potential effects of lyophilized fruits of different cultivars of Vaccinium ashei Reade (Family Ericaceae) berries, commonly known as rabbiteye blueberries, on nociception. This was achieved using the formalin, hot plate, tail-flick, and writhing tests in mice. During this experiment the mice consumed approximately 3.2–6.4mg/kg/day (p.o.) of the anthocyanins. The extract was administered for 21 days or 60 minutes before test. Morphine and diclofenac (10mg/kg, p.o.) as the standard drug (positive control) and water (via oral gavage) as the negative control were administered before all tests. The blueberry extract produced a significant decrease in constrictions induced by acetic acid and caused graded inhibition of the second phase of formalin-induced pain. Moreover, in both the hot plate and tail-flick tests, it significantly increased the threshold. These data suggest that the extract from V. ashei produced antinociceptive effects, as demonstrated in the experimental models of nociception in mice. Additional experiments are necessary in order to clarify the true target for the antinociceptive effects of rabbiteye blueberry extract.

THE PORTS OF THE STATE OF SANTA CATARINA (BRAZIL)

ROCHA I O,VIDAL L M,GUTERRES C D 인하대학교 정석물류통상연구원 2012 JOURNAL OF INTERNATIONAL LOGISTICS AND TRADE Vol.10 No.3

Due to the Brazilian large territory, the network of ports was structured in order to to meet the demands at national and global scales. In southern Brazil, more specifically in the State of Santa Catarina, the ports are an inseparable component of the set of characteristics which allowed the state to become, over the 20th century, one of the most industrialized regions in Brazil. The increase in industrial exports of Santa Catarina, mainly after the 1980s, resulted in a more diversified exporting agenda, which benefited from the existing transport infrastructure: roads, airports, rail ways, and ports. This research aimed at identifying and analyzing the main problems regarding ports infrastructure of the state, which affects the large exporting industries. The research methods used were: a) bibliographic and documents review; b) questionnaire survey and semi-structured interviews with a diversified sample including 40 of the largest exporting companies in Santa Catarina; and c) processing and analysis of the data and information collected. The use of questionnaire survey and semi-structured interviews aimed at: eliciting and characterizing the main problems facing the ports infrastructure in the state and identifying the competitive advantages and disadvantages compared to worldwide market competitors.

Genotoxicity and Antigenotoxicity Assessments of the Flavonoid Vitexin by the Drosophila melanogaster Somatic Mutation and Recombination Test

Liliane Menezes Fernandes,Zaira da Rosa Guterres,Igor Vivian Almeida,Veronica Elisa Pimenta Vicentini 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.6

Vitexin is a C-glycosylated flavone found in various medicinal plants with several proven biological properties such as anti-inflammatory, antispasmodic, antimicrobial, cytotoxic, and antioxidant activities. Considering that the human population consumes many foods that contain this flavonoid, the objective of this study was to investigate the genotoxic and the antigenotoxic potentials of vitexin by the SMART (Somatic Mutation and Recombination Test) in Drosophila melanogaster wings, in vivo. To evaluate the genotoxic activity, larvae obtained from standard (ST) and high bioactivation (HB) crosses were chronically treated with different concentrations of vitexin (0.15, 0.3, and 0.6 mM). For the evaluation of antigenotoxic activity, the same vitexin concentrations were associated with two damage inducing agents: doxorubicin (0.2 mM) and benzo[a]pyrene (1.0 mM). The results obtained for genotoxic activity showed that vitexin did not induce a statistically significant increment in the frequency of mutant spots, when compared to control. The results for the evaluation of antigenotoxicity indicated that the flavonoid statistically reduced the frequency of mutant spots, when compared to those treated with only the damage inducing agents. Thus, this article presents results that have demonstrated the antigenotoxic activity of vitexin, which could be applied in new studies for the development of drugs with chemoprotective effects.

Incorporation of amoxicillin-loaded microspheres in mineral trioxide aggregate cement: an in vitro study

Bohns Fábio Rocha,Leitune Vicente Castelo Branco,Garcia Isadora Martini,Genari Bruna,Dornelles Nélio Bairros,Guterres Silvia Stanisçuaski,Ogliari Fabrício Aulo,de Melo Mary Anne Sampaio,Collares Fabrí 대한치과보존학회 2020 Restorative Dentistry & Endodontics Vol.45 No.4

Objectives: In this study, we investigated the potential of amoxicillin-loaded polymeric microspheres to be delivered to tooth root infection sites via a bioactive reparative cement. Materials and Methods: Amoxicillin-loaded microspheres were synthesized by a spray-dray method and incorporated at 2.5% and 5% into a mineral trioxide aggregate cement clinically used to induce a mineralized barrier at the root tip of young permanent teeth with incomplete root development and necrotic pulp. The formulations were modified in liquid:powder ratios and in composition by the microspheres. The optimized formulations were evaluated in vitro for physical and mechanical eligibility. The morphology of microspheres was observed under scanning electron microscopy. Results: The optimized cement formulation containing microspheres at 5% exhibited a delayed-release response and maintained its fundamental functional properties. When mixed with amoxicillin-loaded microspheres, the setting times of both test materials significantly increased. The diametral tensile strength of cement containing microspheres at 5% was similar to control. However, phytic acid had no effect on this outcome (p > 0.05). When mixed with modified liquid:powder ratio, the setting time was significantly longer than that original liquid:powder ratio (p < 0.05). Conclusions: Lack of optimal concentrations of antibiotics at anatomical sites of the dental tissues is a hallmark of recurrent endodontic infections. Therefore, targeting the controlled release of broad-spectrum antibiotics may improve the therapeutic outcomes of current treatments. Overall, these results indicate that the carry of amoxicillin by microspheres could provide an alternative strategy for the local delivery of antibiotics for the management of tooth infections.

Genotoxicity of Turnera subulata and Spondias mombin × Spondias tuberosa Extracts from Brazilian Caatinga Biome

Tiago Felipe Senes-Lopes,Jorge Alberto Lo´pez,Viviane Souza do Amaral,Jose´ Branda˜o-Neto,Adriana Augusto de Rezende,Jefferson Roma´ryo Duarte da Luz,Zaira da Rosa Guterres,Maria das Grac¸as Almeida 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.4

Medicinal plants have been used in primary healthcare since the earliest days of humankind. Turnera subulata and Spondias mombin × Spondias tuberosa are widely used in the Brazilian Northeast to treat several diseases. The aim of this study was to evaluate the genotoxic effects of the leaf extracts of these species by the somatic mutation and recombination test in the somatic cells of Drosophila melanogaster wings. The experiments were performed using standard and high-bioactivation cross and three concentrations of the test substance [aqueous extract (AET and AES) at 5.0, 10.0, and 20.0 mg/mL and ethanolic extract (EET and EES) and ethyl acetate fraction (EAFT and EAFS) at 0.625, 1.25, and 2.5 mg/mL]. Results indicated that the extracts and fractions induced spontaneous frequencies of mutant spots in both D. melanogaster crosses. Nevertheless, the highest concentrations of the tested plant chemical agents were responsible for the statistically significant genotypic effect. T. subulata and S. mombin × S. tuberosa displayed genotoxic effect under the experimental conditions. The results from this study are crucial as they indicated the deleterious and side effects, considering the indiscriminate use of the extracts of these plants for disease treatment.

Myricitrin from Combretum lanceolatum Exhibits Inhibitory Effect on DNA-Topoisomerase Type IIα and Protective Effect Against In Vivo Doxorubicin-Induced Mutagenicity

Renata Trentin Perdomo,Camila Pineze Defende,Patrick da Silva Mirowski,Talita Vilalva Freire,Simone Schneider Weber,Walmir Silva Garcez,Zaira da Rosa Guterres,Maria de Fatima Cepa Matos,Fernanda Rodri 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.3

Flavonoids—compounds abundant in balanced daily diets—have been extensively investigated for biological activity. The pronounced antiproliferative effects of flavonoids have prompted studies to elucidate their mode of action against tumor cells. The anticancer properties of myricetin, a 3′,4′,5′-tri-hydroxylated flavonol, have been confirmed for a number of neoplasms, but myricitrin, its 3-O-rhamnoside derivative found in fruits and other parts of edible plants, has been scarcely investigated as a chemopreventive agent. This study evaluated the antiproliferative potential of myricitrin obtained from Combretum lanceolatum (Combretaceae) against MCF7 (breast), PC-3 (prostate), HT-29 (colon), 786-0 (kidney), and HL-60 (acute promyelocytic leukemia) cancer cell lines, using the sulforhodamine B and tetrazolium salt assays. Myricitrin proved most effective in inhibiting growth of HL-60 cells (GI50 = 53.4 μmol·L−1), yet showed weak antiproliferative activity against other cell lines. Possible cytotoxic mechanisms involving inhibition of topoisomerases I and IIα by myricitrin were also evaluated, revealing inhibitory activity only against topoisomerase IIα. The results suggested that topoisomerase IIα inhibition is the probable mechanism responsible for the antiproliferative activity of myricitrin. In vivo mutagenicity by myricitrin and its possible antimutagenic effect on doxorubicin-induced DNA damage were also investigated by performing the somatic mutation and recombination test (SMART) on Drosophila melanogaster. Myricitrin proved nonmutagenic to the offspring of standard (ST) and high-bioactivation (HB) crosses, while cotreatments with doxorubicin revealed the antimutagenic properties of myricitrin, even under conditions of high metabolic activation.