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        Enhancement of CO2 desorption using ultrasound and vacuum in water scrubbing biogas upgrading system

        Fuqiang Jin,Haipeng Xu,Dongliang Hua,Lei Chen,Yan Li,Yuxiao Zhao,Bin Zuo 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.1

        Ultrasound and vacuum were respectively employed to enhance CO2 desorption in a water scrubbing biogas upgrading system. Results showed that incomplete CO2 desorption could cause a high CO2 content in the water and seriously affect the purity of the product gas. Vacuum had a strong enhancement effect on CO2 desorption. When a vacuum of 0.04MPa was used to enhance CO2 desorption, the amount of the stripping air could be reduced to 1/16- th of that without enhancement, indicating that vacuum could greatly enhance CO2 desorption and significantly decrease the amount of the stripping air, which was expected to reduce a large amount of energy consumption. In contrast, the enhancement effect of ultrasound was not so obvious for CO2 desorption in the desorption column with air stripping, since the solution could be well desorbed by gas stripping, though ultrasound could strongly affect the static CO2 desorption.

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        Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic

        Pan Jin,Fuqiang Yin,Li Huang,Li Zheng,Jinmin Zhao,XINGDONG ZHANG 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

        Neuro-osteological interactions have an important role in the regulation of bone metabolism and regeneration. Neuropeptides combined with porous biphasic calcium phosphates (BCP) using protein adsorption may contribute to the acceleration of bone formation. In the present study, we investigated the effect of BCP combined with nerve growth factor (NGF) on the growth of osteoblasts in vitro and the combinational therapeutic effect on the repair of calvarial defects in vivo. NGF was separated and purified from Chinese cobra venom using a simplified three-step chromatography method. BCP combined with NGF exerted a potent effect on osteoblast differentiation, as evidenced by enhanced cell proliferation, increased ALP activity and the upregulated expression of osteogenesis-related genes and proteins. Further, combinational therapy with BCP and NGF improved calvarial regeneration, which was superior to treatment with therapy alone, as observed using imageological and morphological examination and histological and immunohistochemical staining. The results confirmed the effect of neuro-osteological interactions through combinatorial treatment with NGF and BCP to promote osteogenesis and bone formation, which may provide an effective and economical strategy for clinical application.

      • SCIESCOPUSKCI등재

        KAT8/MOF-Mediated Anti-Cancer Mechanism of Gemcitabine in Human Bladder Cancer Cells

        ( Huihui Zhu ),( Yong Wang ),( Tao Wei ),( Xiaoming Zhao ),( Fuqiang Li ),( Yana Li ),( Fei Wang ),( Yong Cai ),( Jingji Jin ) 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.2

        Histone acetylation is a well-characterized epigenetic modification controlled by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Imbalanced histone acetylation has been observed in many primary cancers. Therefore, efforts have been made to find drugs or small molecules such as HDAC inhibitors that can revert acetylation levels to normal in cancer cells. We observed dose-dependent reduction in the endogenous and exogenous protein expression levels of KAT8 (also known as human MOF), a member of the MYST family of HATs, and its corresponding histone acetylation at H4K5, H4K8, and H4K16 in chemotherapy drug gemcitabine (GEM)-exposed T24 bladder cancer (BLCA) cells. Interestingly, the reduction in MOF and histone H4 acetylation was inversely proportional to GEM-induced γH2AX, an indicator of chemotherapy drug effectiveness. Furthermore, pGL4-MOF-Luc reporter activities were significantly inhibited by GEM, thereby suggesting that GEM utilizes an MOF-mediated anti-BLCA mechanism of action. In the CCK-8, wound healing assays and Transwell® experiments, the additive effects on cell proliferation and migration were observed in the presence of exogenous MOF and GEM. In addition, the promoted cell sensitivity to GEM by exogenous MOF in BLCA cells was confirmed using an Annexin V-FITC/PI assay. Taken together, our results provide the theoretical basis for elucidating the anti-BLCA mechanism of GEM.

      • Proteomic Analysis and Extensive Protein Identification from Dry, Germinating Arabidopsis Seeds and Young Seedlings

        Fu, Qiang,Wang, Bai-Chen,Jin, Xiang,Li, Hong-Bing,Han, Pei,Wei, Kai-Hua,Zhang, Xue-Min,Zhu, Yu-Xian Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6

        Proteins accumulated in dry, stratified Arabidopsis seeds or young seedlings, totaled 1100 to 1300 depending on the time of sampling, were analyzed by using immobilized pH gradient 2-DE gel electrophoresis. The molecular identities of 437 polypeptides, encoded by 355 independent genes, were determined by MALDI-TOF or TOF-TOF mass spectrometry. In the sum, 293 were present at all stages and 95 were accumulated during the time of radicle protrusion while another 18 appeared in later stages. Further analysis showed that 226 of the identified polypeptides could be located in different metabolic pathways. Proteins involved in carbohydrate, energy and amino acid metabolism constituted to about 1/4, and those involved in metabolism of vitamins and cofactors constituted for about 3% of the total signal intensity in gels prepared from 72 h seedlings. Enzymes related to genetic information processing increased very quickly during early imbibition and reached highest level around 30 h of germination.

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