http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Freed (검색결과 13 건)
- 무료
- 기관 내 무료
- 유료
-
-
Naeem Afifa,Freed Shoaib,Akmal Muhammad 한국곤충학회 2020 Entomological Research Vol.50 No.5
Asiatic citrus psylla, Diaphorina citri, Kuwayama (Hemiptera: Liviidae) is an economic pest of citrus groves and a vector of the bacterium, Candidatus Liberibacter spp., one of the causative agents of citrus greening. In order to estimate the infectivity of six different isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea, fungal bioassay was performed on the adults of D. citri. Adults of D. citri were treated individually with 1 × 105 , 1 × 106 , 1 × 107 , 1 × 108 , 2 × 108 spores/mL fungal concentrations by the immersion method. Subsequent to fungal bioassay, treated D. citri were used to determine the levels of esterase and glutathione S‐transferases (GST) enzymes over a period of 3–7 days. The mortality results suggest that I. fumosorosea isolates (If‐02) caused 82.2% mortality on the seventh day of treatment. However, B. bassiana isolate (Bb‐08) with lowest LC50 (1.4 × 107 spores/mL) proved to be highest potential isolate against D. citri. Biochemical determination of esterase and GST activity assay showed significant differences in activities after infection of fungi. Significantly high activity of esterase was observed by Bb‐01 (27.0 unit per mg protein) on the seventh day, while Ma‐11.1 and If‐2.3 (16.9 and 36.3 unit per mg protein) on the third day post treatment. However, maximum GST's activity was showen by isolates Bb‐08,Ma‐M2 and If‐2.3 (37.6, 1.40 and 10.9 unit per mg protein) on the third day. The current investigation will help to explore the relations between the insect defense system and entomopathogenic fungi. Moreover, the determination of enzymatic activities will be useful for selecting the most pathogenic isolates.
-
Ahmed Rizwan,Freed Shoaib,Naeem Afifa,Akmal Muhammad 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2
Outbreaks of red palm weevil, Rhynchophorus ferrugineus Olivier, in Pakistan are considered a very serious threat to the date industry, owing to the sudden death of date palm trees. Current integrated management strategy largely depends on insecticides, with negative impacts on the environment. Integration of bio-control strategies incorporating entomopathogenic fungi (EPF) may provide an ecologically safe alternative. In the current study the effectiveness of Metarhizium anisopliae Sorokin against R. ferrugineus was investigated using the immersion method and the effects of this fungus on biological parameters of R. ferrugineus were estimated by age-stage, twosex life table software. After treatment with M. anisopliae isolate (Ma-M 2 ), lethal concentration (LC 50 ) 1.2 × 10 6 spores/mL was determined against R. ferrugineus 3rd instar grubs. M. anisopliae infected grubs showed reduced net reproductive rate R o (75.55) and mean generation time T (113.886 days) and intrinsic rate of increase r (0.03797) compared to the untreated group. Also, adult longevity of males and females were significantly reduced in the M. anisopliae-infected group (F 1 ) as compared to the untreated group. Trans-generational studies revealed that grubs stage duration (69.34 days) and fecundity (181.32 eggs) of the M. anisopliae-infected group (F 1 ) were decreased compared to the untreated group. The use of lasting and adverse effects on the biological parameters of R. ferrugineus, indicate that treatment with EPF may provide an eco-friendly management strategy for this pest.
-
Ahmed Rizwan,Freed Shoaib,Naeem Afifa,Akmal Muhammad 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3
Outbreaks of red palm weevil, Rhynchophorus ferrugineus Olivier, in Pakistan are considered a very serious threat to the date industry, owing to the sudden death of date palm trees. Current integrated management strategy largely depends on insecticides, with negative impacts on the environment. Integration of bio-control strategies incorporating entomopathogenic fungi (EPF) may provide an ecologically safe alternative. In the current study the effectiveness of Metarhizium anisopliae Sorokin against R. ferrugineus was investigated using the immersion method and the effects of this fungus on biological parameters of R. ferrugineus were estimated by age-stage, twosex life table software. After treatment with M. anisopliae isolate (Ma-M 2 ), lethal concentration (LC 50 ) 1.2 × 10 6 spores/mL was determined against R. ferrugineus 3rd instar grubs. M. anisopliae infected grubs showed reduced net reproductive rate R o (75.55) and mean generation time T (113.886 days) and intrinsic rate of increase r (0.03797) compared to the untreated group. Also, adult longevity of males and females were significantly reduced in the M. anisopliae-infected group (F 1 ) as compared to the untreated group. Trans-generational studies revealed that grubs stage duration (69.34 days) and fecundity (181.32 eggs) of the M. anisopliae-infected group (F 1 ) were decreased compared to the untreated group. The use of lasting and adverse effects on the biological parameters of R. ferrugineus, indicate that treatment with EPF may provide an eco-friendly management strategy for this pest.
-
Adeel Rehan,Shoaib FREED 한국곤충학회 2015 Entomological Research Vol.45 No.5
Spodoptera litura is one of the most destructive polyphagous insect pests, with more than 120 host‐plant species. In our present study, a field‐collected population of S. litura when selected with spinosad for 11 consecutive generations resulted in the development of 3921‐fold resistance to spinosad as compared to the susceptible strain. The spinosad‐resistant strain of S. litura had a relatively high fitness cost (0.17) as compared to the susceptible strain. Furthermore, the lethal and sub‐lethal effects of different concentrations of spinosad were checked on the susceptible strain at different levels; i.e., LC40, LC30, LC20 and LC10, which revealed that the impact of spinosad on the life‐history traits of S. litura increased with the increase in concentration of spinosad. A significant impact of spinosad was recorded on the larval duration, pre‐pupal weight, pupal duration, pupal weight, reproductive potential and adult emergence. The outcomes of the current research clearly indicate that fitness cost of spinosad and its sub‐lethal effects have a significant impact on population dynamics of S. litura, for which it can be incorporated in integrated pest management.
-
Pathogenecity of insect pathogenic fungus Isaria fumosorosea to different aphid species
Muhammad Naeem Malik,Shoaib Freed,Hafiza Tahira Gul,Muhammad Akmal 단국대학교 국제농업협력연구소 2012 단국대학교 국제농업협력연구소 학술대회 Vol.2012 No.1
In the current research, the insect pathogenic fungus, Is aria fumosorosea was assessed for its pathogenecity against adults of different aphid species of wheat and Brassica crop i.e. Schizaphis graminum, Rhopalosiphum padi, Brevicoryne brassicae and Lipaphis erysimi. I. fumosorosea (IF-01) was found effective at all concentrations i.e. 1x106, 1x107 and 1x108 spores/ml on all aphid species, but the uppermost concentration (lx108 spores/ml) provided maximum control within short period of time (P < 0.05). The results showed that the effect of I. fumosorosea on different aphid species was dose and time dependent. I. fumosorosea with LC50 value of 9.02 x 105 proved to be lethal to L. erysimi after 3rd day. Mortality of B. brassicae was observed maximum on highest concentration of 1x108 spores/ml with LC50 value of 1.64 x 107 , while the LT50 values were in range of 1.78-4.53 days for different aphid species treated at various concentrations. By checking the field efficacy of I. fumosorosea against aphid species, this entomopathogenic fungus can be used as possible biological control agent for suppressing aphids.
-
Muhammad Bilal,Shoaib FREED,Saleh MUHAMMAD,Muhammad Zubair ASHRAF,Muhammad Bismillah KHAN 한국곤충학회 2018 Entomological Research Vol.48 No.4
Helicoverpa armigera, a polyphagous insect of crops and vegetables, is acquiring resistance against many commercial insecticides. The present study shows variations in the activity of two detoxification enzymes, namely esterase and glutathione S‐transferase (GST), in H. armigera after exposure to different isolates of entomopathogenic fungi. After treatment of larvae with the different isolates (Day 0), samples were collected on three days (Days 3, 5 and 7) for enzyme analysis. High GST activity was found in samples of hemolymph, intestine and fat bodies of H. armigera following treatment with Beauveria bassiana (isolate Bb‐08), Metarhizium anisopliae (isolates Ma‐11.1 and Ma‐4.1), and Isaria fumosorosea (isolates If‐02 and If‐2.3). High esterase activity was recorded in samples of the intestine and fat bodies on various days after treatment, whereas increased esterase activity in hemolymph was noted only in samples from Day 5 after treatment with M. anisopliae (Ma‐4.1). The detection of high GST and esterase activity demonstrates the possibility of the development of resistance against these microbial control agents in H. armigera.
-
Brain Tumor Prediction through Behavior Analysis of Cells Growth Using Machine Learning Techniques
Nouh Sabri Elmitwally,Muhammad Aqib Freed,Muhammad Waseem Iqbal,Aasma Ashraf,Farukh Muneem,Muhammad Aqeel 한국차세대컴퓨팅학회 2022 한국차세대컴퓨팅학회 학술대회 Vol.2022 No.10
Brain tumor is a very terrible disease. Brain tumor is caused by an increased number of cells. The presence of the skull layer around the brain makes it tough in studying the behavior of growth cells. It also raises the complication for the identification of disease. The initial discovery of a brain tumor is necessary to defend the survival of patients. Frequently, the brain cancer segmentation, and classification through the MRI images technique. Though, the radiologists are not providing actual visualization of brain cells in MRI images due to the irregular growth of cells, which forms of cells are growing rapidly and slow at some stage in brain tumors in the brain. So, automatic strategies are required to evaluate thoughts tumors exactly from MRI images in this research automatic, MRI brain tumors are used for classification, segmentation, and Behavior analysis of cell growth. The problem of visualization of cell growth and behavior analysis of brain cells is solved through MRI images which enhance the detection of cancer. To analyze the behavior of cell growth, which forms of cells are growing rapidly and slow at some stage in brain tumors, and analyze the area of images in which type of cells is affected. Single models are less efficient. We will use ensemble models which would also be helpful for better performance and accuracy.
-
Lee, Yoon-Sup,Sun, Ho-Sung,Freed, Karl F.,Hagstrom, S. Korean Chemical Society 1986 Bulletin of the Korean Chemical Society Vol.7 No.4
A multi-reference many-body perturbation theory (MRMBPT) method is critically tested in second order by comparing with the corresponding configuration interaction (CI) calculations. Excitation energies of the four-valence-electron states of transition metal atoms and ions are used for the comparison. The agreement between the second order MRMBPT and CI calculations is very reasonable, confirming the reliability of the second order MRMBPT method. The reliability of calculations with the present second order MRMBPT method was only been inferred empirically in the past since most results have been gauged by the agreement with experiment and/or with other MRMBPT calculations based upon different sets of orbitals and configuration spaces. The present MRMBPT method appears to be an efficient ab initio multi-reference method for the calculation of electron correlation effects in atoms and molecules, and it is shown how MRMBPT can be used to estimate core-core and core-valence correlation effects which are often omitted in CI calculations because too many configurations and correlating electrons are involved.
-
Benchmarking all-atom simulations using hydrogen exchange
Skinner, John J.,Yu, Wookyung,Gichana, Elizabeth K.,Baxa, Michael C.,Hinshaw, James R.,Freed, Karl F.,Sosnick, Tobin R. National Academy of Sciences 2014 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.111 No.45
<P><B>Significance</B></P><P>Molecular dynamics simulations have recently become capable of observing multiple protein unfolding and refolding events in a single-millisecond–long trajectory. This major advance produces atomic-level information with nanosecond resolution, a feat unmatched by experimental methods. Such simulations are being extensively analyzed to assess their description of protein folding, yet the results remain difficult to validate experimentally. We apply a combination of hydrogen exchange, NMR, and other techniques to test the simulations with a resolution of single H-bonds. Several significant discrepancies between the simulations and experimental data were uncovered for regions of the energy surface outside of the native basin. This comparison yields suggestions for improving the force fields and provides a general method for experimentally validating folding simulations.</P><P>Long-time molecular dynamics (MD) simulations are now able to fold small proteins reversibly to their native structures [Lindorff-Larsen K, Piana S, Dror RO, Shaw DE (2011) <I>Science</I> 334(6055):517–520]. These results indicate that modern force fields can reproduce the energy surface near the native structure. To test how well the force fields recapitulate the other regions of the energy surface, MD trajectories for a variant of protein G are compared with data from site-resolved hydrogen exchange (HX) and other biophysical measurements. Because HX monitors the breaking of individual H-bonds, this experimental technique identifies the stability and H-bond content of excited states, thus enabling quantitative comparison with the simulations. Contrary to experimental findings of a cooperative, all-or-none unfolding process, the simulated denatured state ensemble, on average, is highly collapsed with some transient or persistent native 2° structure. The MD trajectories of this protein G variant and other small proteins exhibit excessive intramolecular H-bonding even for the most expanded conformations, suggesting that the force fields require improvements in describing H-bonding and backbone hydration. Moreover, these comparisons provide a general protocol for validating the ability of simulations to accurately capture rare structural fluctuations.</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
