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Estudios sobre Asia y Corea en Ia Universidad Complutense de Madrid : Informe
Rodao, Florentino 서울大學校 스페인中南美硏究所 1996 이베로아메리카硏究 Vol.7 No.-
Es un honor poder participar en este foro, no solo por la alegria de estar con ustedes, por hacer nuevos amigos y por volver a ver antiguas caras, sino tambie´n por hablar de algo complementario a lo que esta´n realizando ustedes en su carrera profesional. Me explico: si bien ustedes esta´n impulsando el conocimiento de España en Asia, yo quisiera hablar de lo contrario, de los intentos de conocer Asia en España. No so´lo hay asia´ticos hispanistas sino españoles asianistas, aunque no se´ si esta palabra me la admitira´n los filo´logos aqui presentes.
Influence of NaOH concentration on the decolorization of crystal violet dyed cotton fabric
Kristen Mardenborough,Maya Florentino,Robert Haxhari,Yu-Chung Lin,Miriam Rafailovich,Gary Halada,Hye Jung Jung,Taejin Kim 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.2
Synthetic dye removal is a topic of increasing interest as textile recycling has become more popular in industries. While methods involving dye removal from wastewater effluent have been widely studied and reported on, research on decolorization of fabric itself remains quite unknown. In regard to the lack of research, this study presents cotton fabric samples dyed with crystal violet (CV) that were treated with varying concentrations of sodium hydroxide (NaOH). Fabric decolorization was studied using several characterization methods. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy data showed that the cellulose structure remained unchanged after CV and NaOH treatment. Characteristic CV peaks in the FTIR and Raman spectra were apparent only in the control sample, while the spectra of NaOH-treated samples were very similar to that of the cotton fabric. X-ray diffractometry (XRD) data also confirmed that the crystallite size of cellulose was not affected by CV and NaOH treatment. A visible violet hue remained in all NaOH-treated samples, though CV intensity was inversely proportional to NaOH concentration. The L<SUP>*</SUP>a<SUP>*</SUP>b<SUP>*</SUP> values were utilized to complement characterization results. As the concentration of NaOH was increased, the CIELAB parameters aligned more with those of the plain untreated fabric.
Guerreiro, Marisa M.,Serranheira, Florentino,Cruz, Eduardo B.,Sousa-Uva, Antonio Occupational Safety and Health Research Institute 2020 Safety and health at work Vol.11 No.4
Background: Assembly lines work is frequently associated to work-related upper limb musculoskeletal disorders. The related disability and absenteeism make it important to implement efficient health surveillance systems. The main objective of this study was to identify self-reported variables that can determine work-related upper limb musculoskeletal symptoms-discomfort/pain-during a 6-month follow-up. Methods: This was a prospective study with a 6-month follow-up period, performed in an assembly line. Upper limb musculoskeletal discomfort/pain was assessed through the presence of self-reported symptoms. Uni- and multivariate logistic regression analyses were used to evaluate which self-reported variables were associated to upper limb symptoms after 6 months at the present and to upper limbs symptoms in the past month. Results: Of the 200 workers at baseline, 145 replied to the survey after 6 months. For both outcomes, "having upper limb symptoms during the previous 6 months" and "education" were possible predictors. Conclusion: Our results suggest that having previous upper limb symptoms was related to its maintenance after 6 months, sustaining it as a specific determinant. It can be a hypothesis that this population had mainly workers with chronic symptoms, although our results give only limited support to self-reported indicators as determinants for upper limb symptoms. Nevertheless, the development of an efficient health surveillance system for high demanding jobs should implicate self-reported indicators, but also clinical and work conditions assessment should be accounted on the future.
De Dios Perez, Juan,Santos, Florentino Garcia Department of Mathematics 2009 Kyungpook mathematical journal Vol.49 No.2
We classify real hypersurfaces in complex projective space whose structure Jacobi operator satisfies a certain cyclic condition.
Feeder Cells Free Rabbit Oral Mucosa Epithelial Cell Sheet Engineering
Joan Oliva,Ken Ochiai,Arjie Florentino,Fawzia Bardag-Gorce,Andrew Wood,Yutaka Niihara 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.3
The optimal cell culture method of autologous oral mucosal epithelial cell sheet is not well established for a safe transplantation on to the patients’ ocular surface. Animal serum and 3T3 mouse feeder cells are currently being used to stimulate the growth of the epithelial cells. However, the use of animal compounds can have potential side effects for the patient after transplantation of the engineered cell sheet. In the present study, we focused on engineering a rabbit oral mucosal epithelial cell sheet without 3T3 mouse feeder cells using a mix of Dulbecco’s Modified Eagle Medium/Bronchial Epithelial Cell Growth Medium culture media (DMEM/BEGM). Autologous oral mucosal epithelial cell sheets, engineered with DMEM/BEGM feeder cell free culture media, were compared to those cultured in presence of serum and feeder cells. Using a DMEM/BEGM mix culture media, feeder cell free culture condition, autologous oral mucosal epithelial cells reached confluence and formed a multilayered sheet. The phenotype of engineered cell sheets cultured with DMEM/BEGM were characterized and compared to those cultured with serum and feeder. Hematoxylin and eosin staining showed the formation of a similar stratified multilayer cell sheets, in both culture conditions. The expression of deltaN-p63, ABCG2, PCNA, E-cadherin, Beta-catenin, CK3, CK4, CK13, Muc5AC, was similar in both culture conditions. We demonstrated that rabbit autologous oral mucosal epithelial cell sheet can be engineered, in feeder cell free conditions. The use of the DMEM/BEGM culture media to engineer culture autologous oral mucosa epithelial cell sheet will help to identify key factors involved in the growth and differentiation of oral mucosal epithelial cells.
Abdul Hakim Mohamed Salleh,Mohd Saberi Mohamad,Safaai Deris,Sigeru Omatu,Florentino Fdez-Riverola,Juan Manuel Corchado 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
The increasing demand of biochemical supply for various industries has spurred the development of metabolic engineering to find the optimal design of the microbial cell factories. Traditional method of chemical synthesis using the natural producer leads to the production far below their theoretical maximums. Gene knockout strategy is then introduced to improve the metabolite production. To aid the process, many computational algorithms have been developed to design the optimal microbial strain as cell factories to increase the production of the desired metabolite. However, due to the size of the genome scale model of the microbial strain, finding the optimal combination of genes to be knocked out is not an easy task. In this paper, we propose a hybrid of Genetic Ant Colony Optimization (GACO) and Flux Balance Analysis (FBA) namely GACOFBA to find the optimal gene knockout that increase the production of the target metabolite. Using E. coli and S. cerevisiae genome scale model, we test our proposed hybrid algorithm to increase the production of four different metabolites. By comparing with the results from existing method OptKnock as well as the conventional Ant Colony Optimization (ACO), the results show that our proposed hybrid algorithm able to identify the best set of genes and increase the production while maintaining the optimal growth rate.
REAL HYPERSURFACES IN COMPLEX SPACE FORMS WITH ε-PARALLEL RICCI TENSOR AND STRUCTURE JACOBI OPERATOR
Ki, U-Hang,Perez Juan De Dios,Santos Florentino G.,Suh Young-Jin Korean Mathematical Society 2007 대한수학회지 Vol.44 No.2
We know that there are no real hypersurfaces with parallel Ricci tensor or parallel structure Jacobi operator in a nonflat complex space form (See [4], [6], [10] and [11]). In this paper we investigate real hypersurfaces M in a nonflat complex space form $M_n(c)$ under the condition that ${\nabla}_{\varepsilon}S=0\;and\;{\nabla}_{\varepsilon}R_{\varepsilon}=0,\;where\;S\;and\;R_{\varepsilon}$ respectively denote the Ricci tensor and the structure Jacobi operator of M in $M_n(c)$.
da Silva Emmanuel João Nogueira Leal,de Moura Sara Gomes,de Lima Carolina Oliveira,Barbosa Ana Flávia Almeida,Misael Waleska Florentino,Lacerda Mariane Floriano Lopes Santos,Sassone Luciana Moura 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.2
Objectives: The aim of this study was to evaluate the shaping ability of the TruShape and Reciproc Blue systems and the apical extrusion of debris after root canal instrumentation. The ProTaper Universal system was used as a reference for comparison. Materials and Methods: Thirty-three mandibular premolars with a single canal were scanned using micro-computed tomography and were matched into 3 groups (n = 11) according to the instrumentation system: TruShape, Reciproc Blue and ProTaper Universal. The teeth were accessed and mounted in an apparatus with agarose gel, which simulated apical resistance provided by the periapical tissue and enabled the collection of apically extruded debris. During root canal preparation, 2.5% sodium hypochlorite was used as an irrigant. The samples were scanned again after instrumentation. The percentage of unprepared area, removed dentin, and volume of apically extruded debris were analyzed. The data were analyzed using 1-way analysis of variance and the Tukey test for multiple comparisons at a 5% significance level. Results: No significant differences in the percentage of unprepared area were observed among the systems (p > 0.05). ProTaper Universal presented a higher percentage of dentin removal than the TruShape and Reciproc Blue systems (p < 0.05). The systems produced similar volumes of apically extruded debris (p > 0.05). Conclusions: All systems caused apically extruded debris, without any significant differences among them. TruShape, Reciproc Blue, and ProTaper Universal presented similar percentages of unprepared area after root canal instrumentation; however, ProTaper Universal was associated with higher dentin removal than the other systems.