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Analysis of the transcripts encoding for antigenic proteins of Bovine Gammaherpesvirus 4
Florencia Romeo,Maximiliano J. Spetter,Pedro Moran,Susana Pereyra,Anselmo Odeon,Sandra E. Perez,Andrea E. Verna 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1
The major glycoproteins of bovine gammaherpesvirus 4 (BoHV-4) are gB, gH, gM, gL, and gp180 with gB, gH, and gp180 being the most glycosylated. These glycoproteins participate in cell binding while some act as neutralization targets. Glycosylation of these envelope proteins may be involved in virion protection against neutralization by antibodies. In infected cattle, BoHV-4 induces an immune response characterized by low neutralizing antibody levels or an absence of such antibodies. Therefore, virus seroneutralization in vitro cannot always be easily demonstrated. The aim of this study was to evaluate the neutralizing capacity of 2 Argentine BoHV-4 strains and to associate those findings with the gene expression profiles of the major envelope glycoproteins. Expression of genes coding for the envelope glycoproteins occurred earlier in cells infected with isolate 10/154 than in cells infected with strain 07/435, demonstrating a distinct difference between the strains. Differences in serological response can be attributed to differences in the expression of antigenic proteins or to post-translational modifications that mask neutralizing epitopes. Strain 07/435 induced significantly high titers of neutralizing antibodies in several animal species in addition to bovines. The most relevant serological differences were observed in adult animals. This is the first comprehensive analysis of the expression kinetics of genes coding for BoHV-4 glycoproteins in 2 Argentine strains (genotypes 1 and 2). The results further elucidate the BoHV-4 life cycle and may also help determine the genetic variability of the strains circulating in Argentina.
Florencia Evelyn,Ria Margiana 대한해부학회 2022 Anatomy & Cell Biology Vol.55 No.4
Anatomy studies require cadavers to study the human body. Generally in Indonesia, the dead human body will be buried. This causes problems because the decomposition process of a cadaver that is preserved with formalin will be delayed and it causes environmental pollution. The toxicity of formalin can be reduced by neutralizing the formalin. This study aimed to compare the decrease of mice mass that were preserved with formalin then neutralized with sodium bicarbonate and those that were not neutralized. This study used 18 mice (Mus musculus) which were divided into 3 groups. They were the control group (not given preservative), group preserved with 4% formalin, and group preserved with 4% formalin then neutralized with sodium bicarbonate. All groups of mice were buried for 6 weeks. The changes in mass were assessed with an analysis of the percentage loss in mass. Based on the results of this study, the formalin group had a greater percentage of total mass reduction than the neutralize group. The formalin group had a higher decomposition rate than the neutralizing sodium bicarbonate group. The effectiveness of reducing the concentration of formalin is similar with neutralize group. Therefore, it can be concluded that 4% formalin is recommended for use to accelerate the occurrence of decay and decrease in mass.
( Florencia Giliberto ),( Veronica Ferreiro ),( Viviana Dalamon ),( Ezequiel Surace ),( Javier Cotignola ),( Sebastian Esperante ),( Daniel Borelina ),( Sergio Baranzini ),( Irene Szijan ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.2
Duchenne muscular dystrophy (DMD) is the most common hereditary neuromuscular disease. It is inherited as an X-linked recessive trait in which males show clinical manifestations. In some rare cases, the disease can also be manifested in females. The aim of the present study was to determine the molecular alteration in two cases of non-related DMD symptomatic carriers with no previous history of DMD. Multiplex PCR is commonly used to search for deletion in the DMD gene of affected males. This method could not be used in females because the normal X chromosome masks the deletion of the mutated one. Therefore, we used a set of seven highly polymorphic dinucleotide (CA), repeat markers that lie within the human dystrophin gene. The deletions were evidenced by hemizygosity of the loci under study. We localized a deletion in the locus 7A (intron 7) on the maternal X chromosome in one case, and a deletion in the region of introns 49 and 50 on the paternal X chromosome in the other. The use of microsatellite genotyping within the DMD gene enables the detection of the mutant allele in female carriers. It is also a useful method to provide DMD families with more accurate genetic counseling.
Florencia Anabel Mesas,Marí,a Cecilia Terrile,Marí,a Ximena Silveyra,Adriana Zuñ,iga,Marí,a Susana Rodriguez,Claudia Anahí,Casalongué,Julieta René,e Mendieta 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.6
Chitosan has been considered an environmental-friend- ly polymer. However, its use in agriculture has not been extended yet due to its relatively low solubility in water. N-Methylene phosphonic chitosan (NMPC) is a water- soluble derivative prepared by adding a phosphonic group to chitosan. This study demonstrates that NMPC has a fungicidal effect on the phytopathogenic fungus Fusarium solani f. sp. eumartii (F. eumartii) judged by the inhibition of F. eumartti mycelial growth and spore germination. NMPC affected fungal membrane per- meability, reactive oxygen species production, and cell death. Also, this chitosan-derivative exerted antifungal effects against two other phytopathogens, Botrytis cine- rea, and Phytophthora infestans. NMPC did not affect tomato cell viability at the same doses applied to these phytopathogens to exert fungicide action. In addition to water solubility, the selective biological cytotoxicity of NMPC adds value in its application as an antimicrobial agent in agriculture.
Fragile-X Mental Retardation: Molecular Diagnosis in Argentine Patients
Florencia, Giliberto,Irene, Szijan,Veronica, Ferreiro Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6
Fragile-X-syndrome (FXS) is the most common type of inherited cognitive impairment. The underlying molecular alteration consists of a CGG-repeat amplification within the FMR-1 gene. The phenotype is only apparent once a threshold in the number of repeats has been exceeded (full mutation). The aim of this study was to characterize the FMR-1 CGG-repeat status in Argentine patients exhibiting mental retardation. A total of 330 blood samples from patients were analyzed by PCR and Southern blot analysis. Initially, DNA from 78 affected individuals were studied by PCR. Since this method is unable to detect high molecular weight alleles, however, we undertook a second approach using the Southern blotting technique to analyze the CGG repeat number and methylation status. Southern blot analysis showed an altered pattern in 14 out of 240 (6%) unrelated patients, with half of them presenting a mosaic pattern. Eight out of 17 families (47%) showed a (suggest deleting highlight). The characteristic FXS pattern was identified in 8/17 families (47%), and in 4 of these families 25% of the individuals presented with a mosaic model. The expansion from pre-mutation to full mutation was shown to occur both at the pre and post zygotic levels. The detection of FXS mutations has allowed us to offer more informed genetic counseling, prenatal diagnosis and reliable patient follow-up.
Liminal but Competent: Latin American Migrant Children and School in Australia
María Florencia Amigó 한국아동학회 2012 Child studies in Asia-Pacific context Vol.2 No.1
Indisputably school is the main institution that socialises migrant children into the culture of their new country. Through school they learn the new language and customs, which will enable them to become cultural brokers between the new cultural world and their families. During this process migrant children often transit a liminal terrain where their roles and identity become at the same time diverse and ambivalent. Despite the challenges involved these children often become experts in juggling different cultures, environments and expectations. This study explores the experiences of Latin American children and their families as they start primary school in Australia.
Giliberto, Florencia,Ferreiro, Veronica,Dalamon, Viviana,Surace, Ezequiel,Cotignola, Javier,Esperante, Sebastian,Borelina, Daniel,Baranzini, Sergio,Szijan, Irene Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.2
Duchenne muscular dystrophy (DMD) is the most common hereditary neuromuscular disease. It is inherited manifestations. In some rare cases, the disease can also be manifested in females. The aim of the present study was to determine the molecular alteration in two cases of nonrelated DMD symptomatic carriers with no previous history of DMD. Multiplex PCR is commonly used to search for deletion in the DMD gene of affected males. This method could not be used in females because the normal X chromosome masks the deletion of the mutated one. Therefor, we used a set of seven highly polymorphic dinucleotide $(CA)_n$ repeat markers that lie within the human dystrophin gene. The deletions were evidenced by hemizygosity of the loci under study. We localized a deletion in the locus 7A (intron 7) on the maternal X chromosome in one case, and a deletion in the region of introns 49 and 50 on the paternal X chromosome in the other. The use of microsatellite genotyping within the DMD gene enables the detection of the mutant allele in female carriers. It is also a useful method to provide DMD families with more accurate genetic counseling.
Liminal but Competent: Latin American Migrant Children and School in Australia
Maria Florencia Amigo 한국아동학회 2012 Child studies in Asia-Pacific context Vol.2 No.1
Indisputably school is the main institution that socialises migrant children into the culture of their new country. Through school they learn the new language and customs, which will enable them to become cultural brokers between the new cultural world and their families. During this process migrant children often transit a liminal terrain where their roles and identity become at the same time diverse and ambivalent. Despite the challenges involved these children often become experts in juggling different cultures, environments and expectations. This study explores the experiences of Latin American children and their families as they start primary school in Australia.
Reinnervated Split-Muscle Technique for Creating Additional Myoelectric Sites in an Animal Model
Deslivia, Maria Florencia,Lee, Hyun-joo,Zulkarnain, Rizki Fajar,Zhu, Bin,Adikrishna, Arnold,Jeon, In-ho,Kim, Keehoon Williams & Wilkins 2016 Plastic and reconstructive surgery Vol.138 No.6
<P>Background: This study proposes a novel reinnervated split-muscle operation to create additional myoelectric sites as sources of command signals of myoelectric prostheses for enhanced dexterous hand-to-wrist motions. The aim of this study was to investigate the postprocedure electromyographic properties of the muscles as distinct myoelectric sites in a rat model. Methods: The reinnervated split-muscle group (n = 6) had the gastrocnemius muscle separated along its longitudinal axis and nerves transferred to each new muscle (peroneal nerve to lateral muscle head and tibial to medial one); the non-split-muscle group (n = 6) only had nerve transfers with its muscle intact. Functional testing was conducted after 10 weeks. The main parameter is the difference in mean electromyographic amplitude between the new muscles, with greater values indicating better separability. Results: After the reinnervated split-muscle procedure, there is a significant increase of the average ratio between two muscles compared with the control group, from 0.44 (range, 0.02 to 0.86) to 0.77 (range, 0.35 to 0.98) (p = 0.011). In addition, compared with the non-split muscle group, nerve transfer in the split-muscle group is more successful in reaching its intended target muscle. Conclusion: A reinnervated split-muscle procedure could be beneficial for acquiring a more precise and discrete command signal in upper limb amputees, thus enabling the creation of more dexterous prosthetic arm.</P>