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Screening of Yeasts Isolated from Brazilian Environments for the 2-Phenylethanol (2-PE) Production
Lorena Azevedo de Lima,Raphael Hermano Santos Diniz,Marisa Vieira de Queiroz,Luciano Gomes Fietto,Wendel Batista da Silveira 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.3
Phenylethanol alcohol, or 2-phenylethanol (2-PE) production by yeasts has been considered a promising alternative to its chemical synthesis. In order to evaluate the potential of yeast strains isolated from different Brazilian environments, we evaluated the 2-PE production of 267 strains. Among them, the Kluyveromyces marxianus CCT 7735 yeast stood out as being the best 2-PE producer. The K. marxianus CCT 7735 growth was impaired by 2-PE; nevertheless, this effect is less pronounced than the inhibition reported for certain Saccharomyces cerevisiae strains. The maximum 2-PE titer obtained under optimized conditions was 3.44 g/L, 28% higher than the titer achieved under unoptimized conditions. The optimized conditions were: 30ºC, and glucose and L-phe concentrations of 3.0 and 4.0 g/L, respectively. Moreover, the specific production rate of 2-PE increased twofold compared to the unoptimized conditions.
Priscila Gonçalves Ferreira,Fernando Augusto da Silveira,Raquel Cristina Vieira dos Santos,Hugo Leonardo André Genier,Raphael Hermano Santos Diniz,José Ivo Ribeiro Júnior,Luciano Gomes Fietto,Flávia M 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
The simultaneous saccharification and fermentation (SSF) process is a promising strategy to obtain ethanol from cellulosic biomass. In this study, sugarcane bagasse was supplemented with ricotta whey to increase the sugar, vitamin, and trace metal concentrations in the fermentation medium. The optimum conditions for SSF ethanol production from a mixture of sugarcane bagasse and ricotta whey produced by Kluyveromyces marxianus CCT 7735 were evaluated considering five factors: cellulase concentration, cellulosic biomass concentration, pH, temperature, and agitation. The highest ethanol yield was 49.65 g/L with a cellulosic biomass of 80 g/L, pH value of 5.05, agitation at 65 rpm and temperature of 39.2ºC. The results demonstrated that a mixture of the cellulosic residue of sugarcane bagasse and ricotta whey is promising for ethanol production because the ethanol yield in the mixture was higher than that in single substrate of sugarcane bagasse.