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      • SCOPUSKCI등재

        Psychosocial Risks Assessment in Cryopreservation Laboratories

        Fernandes, Ana,Figueiredo, Margarida,Ribeiro, Jorge,Neves, Jose,Vicente, Henrique Occupational Safety and Health Research Institute 2020 Safety and health at work Vol.11 No.4

        Background: Psychosocial risks are increasingly a type of risk analyzed in organizations beyond chemical, physical, and biological risks. To this type of risk, a greater attention has been given following the update of ISO 9001: 2015, more precisely the requirement 7.1.4 for the process operation environment. The update of this normative reference was intended to approximate OHSAS 18001: 2007 reference updated in 2018 with the publication of ISO 45001. Thus, the organizations are increasingly committed to achieving and demonstrating good occupational health and safety performance. Methods: The aim of this study was to characterize the psychosocial risks in a cryopreservation laboratory and to develop a predictive model for psychosocial risk management. The methodology followed to collect the information was the inquiry by questionnaire that was applied to a sample comprising 200 employees. Results: The results show that most of the respondents are aware of the psychosocial risks, identifying interpersonal relationships and emotional feelings as the main factors that lead to this type of risks. Furthermore, terms such as lack of resources, working hours, lab equipment, stress, and precariousness show strong correlation with psychosocial risks. The model presented in this study, based on artificial neural networks, exhibited good performance in the prediction of the psychosocial risks. Conclusion: This work presents the development of an intelligent system that allows identifying the weaknesses of the organization and contributing to the enhancement of the psychosocial risks management.

      • SCIESCOPUSKCI등재

        Numerical estimation of ion transport and electroosmotic flow around a pair of cylindrical electrodes in a microchannel using immersed boundary method

        Fernandes, Dolfred Vijay,Kang, Sang-Mo,Suh, Yong-Kweon 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.12

        This paper investigates the ion transport and electroosmotically induced flow around the cylindrical electrodes under both direct current (DC) and alternating current (AC) fields. The Poisson-Nernst-Plank (PNP) equations governing the ion transport around the ideally polarizable electrodes are solved numerically by neglecting the Stem layer effect. The fractional-step (FS) based decoupled solver is used in time integration of the ion-transport equations. A new immersed boundary (IB) methodology is described for imposing no-flux boundary conditions of ion concentration on the electrodes. A fully implicit coupled solver is also developed for calculating the ion transport around a pair of rectangular electrodes. The validity of the decoupled solver is verified by comparing its results with those obtained from the coupled solver. For further confirmation of the validity, the results are also compared with those obtained from the Poisson-Boltzmann model and both results are found to be in excellent agreement. The electroosmotically induced flow field is studied by numerically solving the Stokes equations. The system attains a steady state under DC, where the conduction term of ion transport is balanced by the diffusion term. Until the system attains a steady state for a few ms for the case of DC, fluid flow is induced. The electroosmotic flow under AC is more interesting, in that instantaneous flow oscillates with the frequency double of the applied field and a nonzero steady velocity field persists.

      • SCIESCOPUS

        Finite element analysis of helmeted oblique impacts and head injury evaluation with a commercial road helmet

        Fernandes, Fabio A.O.,de Sousa, R.J. Alves Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.48 No.5

        In this work, the safety performance of a commercial motorcycle helmet already placed on the market is assessed. The assessed motorcycle helmet is currently homologated by several relevant motorcycle standards. Impacts including translational and rotational motions are accurately simulated through a finite element numerical framework. The developed model was validated against experimental results: firstly, a validation concerning the constitutive model for the expanded polystyrene, the material responsible for energy absorption during impact; secondly, a validation regarding the acceleration measured at the headform's centre of gravity during the linear impacts defined in the ECE R22.05 standard. Both were successfully validated. After model validation, an oblique impact was simulated and the results were compared against head injury thresholds in order to predict the resultant head injuries. From this comparison, it was concluded that brain injuries such as concussion and diffuse axonal injury may occur even with a helmet certified by the majority of the motorcycle helmet standards. Unfortunately, these standards currently do not contemplate rotational components of acceleration. Conclusion points out to a strong recommendation on the necessity of including rotational motion in forthcoming motorcycle helmet standards and improving the current test procedures and head injury criteria used by the standards, to improve the safety between the motorcyclists.

      • KCI등재

        Effect of dental bleaching on the microhardness and surface roughness of sealed composite resins

        Fernandes, Renan Aparecido,Strazzi-Sahyon, Henrico Badaoui,Suzuki, Thais Yumi Umeda,Briso, Andre Luiz Fraga,Santos, Paulo Henrique dos The Korean Academy of Conservative Dentistry 2020 Restorative Dentistry & Endodontics Vol.45 No.1

        Objectives: The aim of this in vitro study was to evaluate the microhardness and surface roughness of composite resins before and after tooth bleaching procedures. Materials and Methods: Sixty specimens were prepared of each composite resin (Filtek Supreme XT and Opallis), and BisCover LV surface sealant was applied to half of the specimens. Thirty enamel samples were obtained from the buccal and lingual surfaces of human molars for use as the control group. The surface roughness and microhardness were measured before and after bleaching procedures with 35% hydrogen peroxide or 16% carbamide (n = 10). Data were analyzed using 1-way analysis of variance and the Fisher test (α = 0.05). Results: Neither hydrogen peroxide nor carbamide peroxide treatment significantly altered the hardness of the composite resins, regardless of surface sealant application; however, both treatments significantly decreased the hardness of the tooth samples (p < 0.05). The bleaching did not cause any change in surface roughness, with the exception of the unsealed Opallis composite resin and dental enamel, both of which displayed an increase in surface roughness after bleaching with carbamide peroxide (p < 0.05). Conclusions: The microhardness and surface roughness of enamel and Opallis composite resin were influenced by bleaching procedures.

      • THE PRODUCTION OF CANCERIGENIC SUBSTANCES BY CERTAIN BACTERIA

        Fernand, LOT 中央醫學社 1970 中央醫學 Vol.18 No.1

        For about fifteen years Dr. Lucien Mallet has been making a thorough investigation of the pollution of vital media on land and at sea by polybenzene hydrocarbons of the 3-4 benzopyrene type, which are known to be particularly cancerigenic. He has concluded that apart from the commonly known pollution due to industrial activity, another form exists that is natural in origin and produced by certain bacteria that have proved capable of synthesizing hydrocarbons of this kind. Several scientists have collaborated on this research, headed by Mme M. Heros, of the Prefecture de Police Laboratory, which is particularly interested in problems of pollution (Professors Henri Moureu and Paul Chovin), and Dr. Tissier, technical director of the vaccine section of the Laboratoire de la Sante Publique. The following have also contributed to these investigations: the Institute Medico-Legal (Professor Piedelievra), the Hydrographical Department of the Navy (Chief Engineer Goudeheim), the Meteorological Office, the Marine Biology Laboratory at Dinard directed by Professor Roger Heim(of the Museum), the Villefranche-sur-Mer Zoological Station, the Pont- et- Chaussees (Public Works) Laboratory at Fatouville, the Charbonnages de France(French Mines) The 'list is not complete but gives some idea of the scope of the work, which has involved prospection of many sites-forests, the Seine Estuary, the Mediterranean coasts, and the Davis Straits among others. To start with, two laboratory observations: 3-4 benzopyrene could be fixed by B.C.G.; anaerobic bacteria (living without air), like clostridium putride, fixed it selectively. In view of the remarkably high affinity of the plankton medium for these hydrocarbons and the fact that the lipids present in the organisms of vegetable plankton are solvents of these hydrocarbons, it night be wondered whether such bacteria, particularly the one living without air, do not synthesize them spontaneously. A first proof would be their presence in old sediments cut off from any form of outside pollution. Their presence was discovered in 19 64 by Dr. Mallet and Charles Schneider, in the limestone of an underground quarry 50 metres down: the sample (taken electrically, with no risk of contamination, and then analyzed by chromatography and spectrography) contained benzopyrene amounting to 1. 95 micrograms per 100 grams. It was thus legitimate to consider that the substance must be biological in origin and correspond to the plankton flora of the silex and limestone deposits. It has also been found in fragments of carbon containing sediments such as boghead and in sand and mud in greenland waters. It has also been detected in plankton growing thickly in the lagoon of a particularly interesting atoll, Clipperton, in the Pacific and situated about thirteen hundred kilometres from the nearest coasts (Mexico). It is uninhabited and off the busy sea and air routes. It is particularly remote from any pollution connected with industrial sources since it has been completely hemmed in by its ring of coral for the past hundred years. Analyses of samples taken by Dr. Niaussat have shown from 3. 4 to 4 micrograms of benzopyrene per 100 grams. Most significantly, it has been observed that samples of earth from forests in the French, Massif Central, poor in benzopyrene, can be enriched through cultures of clostridium. Other experiments were carried out in the laboratory cultures of anaerobia (clostridium isolated from various coastal planktons) were placed in sterile flasks containing total lipids extracted from marine plankton, with sea water as nutrient broth. The cultures were incubated for two weeks and then samples were treated with formol and analyzed. In all cases, small amounts of 3-4 benzopyrene were detected, ranging from 12 to 800 microgram per 100 gram dry weight. The size of the last figure (800) is worthy of note. These delicate assays were carried out by chromatography and spectrography improved on to a high degree by Mme Heros in the Rue de Dantzig laboratory of the Prefecture de Police. Other series of experiments have shown that the addition of antibiotics which practically eliminate bacteria, checks the formation of benzopyrene or reduces it in amount. The hypothesis as to its biological origin might seem to be incompatible with the fact that coastal plankton is far more liable to pollution than the marine type. But as Professor Brisou has shown, if coastal plankton is frequently polluted by anaerobia, plankton out at sea is far less so and biosynthesis is therefore correspondingly less active in this case. It has also been noted that in the presence of aerobic bacteria, there seems to be a contrary phenomenon operative in nature that destroys cancer producing hydrocarbons. Further work is nowbeing done on this question at the Dinard laboratory by Miss M. L Priou and Michel Leon. It has been observed that when mud is maintained at laboratory temperature and in the presence of clostridium type anaerobic bacteria, it does produce the substance whereas regression is observed when flora has a high percentage of aero bias. It is very possible that a balance is established in nature, so that hydrocarbons always existed and are maintained at a more or less average rate. 3-4 benzopyrene is not a toxic substance. It is dangerous because it attacks adenine, one of the four bases of desoxy-ribonucleic acid. This results in chromosome alterations, disturbances in the message transmitted by RNA and, finally, in the appearance and proliferation of abnormal cells. As post-mortem analyses have revealed, cancerigenic hydrocarbons are present in all the organs, especially in the liver. spleen, kidneys, lungs and bronchial tubes, esophagus. It was hitherto believed that the substance had been introduced from the outside; and it is a fact that no one to-day can escape the pollution invading, the biosphere on an increasing sale. But in the light of recent observations, it appears that auto-pollution must occur in the case of 3-4 benzopyrene type hydrocarbons, particularly in the digestive tract. Especially in the region of the intestine-rich in bacteria of all kinds, some of which are able to synthesize the dangerous substance from fatty acids. Now such endogenous pollution could be counter acted inside the organism itself: treatment would involve action by aerobic bacteria, since as observed above, some seem to be able to check the action of anaerobia that produce benzopyrene, which when eliminated via the kidneys could be determined quantitatively in the urine and thus kept under control. That is the position up to date. If the hypotheses formulated prove to be accurate, as experiments seem to be indicating so far, it will be a discovery of the greatest importance.

      • KCI등재

        Genetic variability and heritability of agronomic traits in a wheat collection used in southern Brazil

        Fernandes Rebeca Catanio,Busanello Carlos,Viana Vívian Ebeling,Venske Eduardo,de Oliveira Victoria Freitas,Lopes Jennifer Luz,da Maia Luciano Carlos,Costa de Oliveira Antonio,Pegoraro Camila 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.3

        Wheat is a staple food for a large part of the world's population and faces a continuous demand for increased productivity. The presence of genetic variability allows the success in developing cultivars with higher yield. The understanding of the genetic variability available is crucial for their efective use in breeding programs. Inheritance of wheat yield components is complex, due to polygenic control and the strong infuence of the environment. Therefore, an accurate heritability estimation can accelerate the selection gains towards increasing wheat yield. Thus, this study aimed to characterize agronomic traits related to yield in a collection of 99 wheat accessions used in Brazil. In addition, the heritability of these traits was estimated. The studied accessions showed variability for yield, which can be explained by the presence of variability in most yield components. Cluster analysis showed that there is variability in wheat genotypes, however, the introduction of new sources of variability in crossing blocks or the induction of mutations is suggested. The broad-sense heritability for grain yield was considered high, as well as for most of the studied agronomic traits, suggesting that it is possible to obtain genetic gain with selection in this environment. However, for greater accuracy, genetic gain values should be estimated and considered when designing selection strategies. Hectoliter weight showed low heritability, demonstrating the complexity of selection for this trait. In summary, this study demonstrates the presence of genetic variability and high heritability for agronomic traits, enabling genetic gain in breeding programs using these wheat accessions.

      • SCIESCOPUSKCI등재

        A NOTE ON BILATERAL SEMIDIRECT PRODUCT DECOMPOSITIONS OF SOME MONOIDS OF ORDER-PRESERVING PARTIAL PERMUTATIONS

        Fernandes, Vitor H.,Quinteiro, Teresa M. Korean Mathematical Society 2016 대한수학회보 Vol.53 No.2

        In this note we consider the monoid $\mathcal{PODI}_n$ of all monotone partial permutations on $\{1,{\ldots},n\}$ and its submonoids $\mathcal{DP}_n$, $\mathcal{POI}_n$ and $\mathcal{ODP}_n$ of all partial isometries, of all order-preserving partial permutations and of all order-preserving partial isometries, respectively. We prove that both the monoids $\mathcal{POI}_n$ and $\mathcal{ODP}_n$ are quotients of bilateral semidirect products of two of their remarkable submonoids, namely of extensive and of co-extensive transformations. Moreover, we show that $\mathcal{PODI}_n$ is a quotient of a semidirect product of $\mathcal{POI}_n$ and the group $\mathcal{C}_2$ of order two and, analogously, $\mathcal{DP}_n$ is a quotient of a semidirect product of $\mathcal{ODP}_n$ and $\mathcal{C}_2$.

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