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Ferid Abdulhafiz,Fatimah Kayat,Suhana Zakaria 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.2
Inducing genetic and morphological variation through conventional method is very difficult. Therefore, mutation induction through in vitro technology brings numerous advantages over the conventional breeding. Thus, the individual shoots (1 ~ 2 cm) were irradiated with gamma rays (10 ~ 70 Gy). The result revealed that the explants treated with higher doses (40, 50, 60, and 70 Gy) showed deleterious effects of ionizing radiation. The highest survival rate among γ treated explants recorded was 71% in 10 Gy treatments while the lowest survivality was 15% in 70 Gy. Lethal dose 50% (LD50) dose was found to be 33 Gy. In the in vitro condition, rooting reponse showed that increase in gamma irradiation dose resulted in the inhibition of root growth. Meanwhile, non-treated explants had the best rooting ability with the maximum number of root per explant (20) within a short period of time (6 days), with the highest root length of (15.1 cm). The longer period in rooting (12 days) and lowest number of root per explant (8) with shortest root length (10.1 cm) were recorded at 30 Gy treatment. The highest shoot length (13.6 cm) was observed at control treatment and the shortest shoot length (10.4 cm) was observed at 30 Gy. In the nursery, lowest leaf number (5) was observed at 30 Gy compared with other treatments. The highest chlorophyll content (49.8) was recorded at 10 Gy treated seedling. Irradiated explants with 10 Gy found to be superior over the control treatment and had positive effects in main growth parameters such as chlorophyll content.
Faun Tail Nevus and Spinal Dysraphism: Cosmetic Improvement with Alexandrite Laser Epilation
Asli Feride Kaptanoglu,Erkan Kaptanoglu 대한피부과학회 2011 Annals of Dermatology Vol.23 No.-
Faun-tail presents as an abnormal lumbosacral hypertrischosis and may be associated with spinal dysrasphism. In addition to the problems due to spinal anomalies, patient’s physico-social life may also be affected. Here, we report a case of 13 years old female patient with Faun-tail in association with sypinal dysraphism, in which cosmetic improvement was achieved with the help of Alexandrite laser. Alexandrite laser can be the method of choice for permanent hair removal method due to its safe, effective and easy to apply properties. (Ann Dermatol 23(S3) S296∼S298,2011)
Abdulhafiz, Ferid,Kayat, Fatimah,Zakaria, Suhana The Korean Society of Plant Biotechnology 2018 식물생명공학회지 Vol.45 No.2
Inducing genetic and morphological variation through conventional method is very difficult. Therefore, mutation induction through in vitro technology brings numerous advantages over the conventional breeding. Thus, the individual shoots (1 ~ 2 cm) were irradiated with gamma rays (10 ~ 70 Gy). The result revealed that the explants treated with higher doses (40, 50, 60, and 70 Gy) showed deleterious effects of ionizing radiation. The highest survival rate among ${\gamma}$ treated explants recorded was 71% in 10 Gy treatments while the lowest survivality was 15% in 70 Gy. Lethal dose 50% ($LD_{50}$) dose was found to be 33 Gy. In the in vitro condition, rooting reponse showed that increase in gamma irradiation dose resulted in the inhibition of root growth. Meanwhile, non-treated explants had the best rooting ability with the maximum number of root per explant (20) within a short period of time (6 days), with the highest root length of (15.1 cm). The longer period in rooting (12 days) and lowest number of root per explant (8) with shortest root length (10.1 cm) were recorded at 30 Gy treatment. The highest shoot length (13.6 cm) was observed at control treatment and the shortest shoot length (10.4 cm) was observed at 30 Gy. In the nursery, lowest leaf number (5) was observed at 30 Gy compared with other treatments. The highest chlorophyll content (49.8) was recorded at 10 Gy treated seedling. Irradiated explants with 10 Gy found to be superior over the control treatment and had positive effects in main growth parameters such as chlorophyll content.
( Asli Feride Kaptanoglu ),( Erkan Kaptanoglu ) 대한피부과학회 2011 Annals of Dermatology Vol.23 No.3s
Faun-tail presents as an abnormal lumbosacral hypertrischosis and may be associated with spinal dysrasphism. In addition to the problems due to spinal anomalies, patient`s physico-social life may also be affected. Here, we report a case of 13 years old female patient with Faun-tail in association with sypinal dysraphism, in which cosmetic improvement was achieved with the help of Alexandrite laser. Alexandrite laser can be the method of choice for permanent hair removal method due to its safe, effective and easy to apply properties. (Ann Dermatol 23(S3) S296~S298, 2011)
Kim, Beom Su,Shkembi, Feride,Lee, Jun Hindawi Publishing Corporation 2017 BioMed research international Vol.2017 No.-
<P>Alendronate (ALN) is a bisphosphonate drug that is widely used for the treatment of osteoporosis. Furthermore, local delivery of ALN has the potential to improve the bone regeneration. This study was designed to investigate an ALN-containing fibrin (fibrin/ALN) gel and evaluate the effect of this gel on both in vitro cellular behavior using human mesenchymal stem cells (hMSCs) and in vivo bone regenerative capacity. Fibrin hydrogels were fabricated using various ALN concentrations (10<SUP>−7</SUP>–10<SUP>−4 </SUP>M) with fibrin glue and the morphology, mechanical properties, and ALN release kinetics were characterized. Proliferation and osteogenic differentiation of and cytotoxicity in fibrin/ALN gel-embedded hMSCs were examined. In vivo bone formation was evaluated using a rabbit calvarial defect model. The fabricated fibrin/ALN gel was transparent with Young's modulus of ~13 kPa, and these properties were not affected by ALN concentration. The in vitro studies showed sustained release of ALN from the fibrin gel and revealed that hMSCs cultured in fibrin/ALN gel showed significantly increased proliferation and osteogenic differentiation. In addition, microcomputed tomography and histological analysis revealed that the newly formed bone was significantly enhanced by implantation of fibrin/ALN gel in a calvarial defect model. These results suggest that fibrin/ALN has the potential to improve bone regeneration.</P>
Combination effect of three anti-HSV-2 active plant extracts exhibiting different modes of action
Benzekri Roudaina,Limam Ferid,Bouslama Lamjed 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.2
The emergence of multidrug-resistant viruses leads to discover a novel source of drugs or a new strategy to overcome this obstacle. Combined herbal therapies are becoming a promising approach for a better efficiency. In this study, Pistacia lentiscus and Peganum harmala were investigated for their anti HSV-2 activity. Twenty eight organic extracts from different organs were evaluated for their cytotoxicity on Vero cells and their anti HSV-2 activity using the MTT test and the plaque reduction assay, respectively. Peganum harmala methanol seeds extract, Pistacia lentiscus methanol stem and dichloromethane fruit extracts were active on HSV-2 with selectivity index values of 13.19, 51.23 and 10.23, respectively. These active extracts exhibited different mode of action: (1) Peganum harmala methanol seeds extract showed a virucidal action before virus adsorption and penetration, and on the newly formed virions before their release outside infected cells—(2) Pistacia lentiscus methanol stem extract exhibited virucidal effect only before virus adsorption and penetration—(3) Pistacia lentiscus dichloromethane fruit extracts demonstrated virus inhibition up to 7 h post infection. The binary combination of the three active extracts did not improve their individually activity. This report could contribute to prove that combination of active extracts do not necessary increase the initial activity.
Lehwald-Tywuschik, Nadja,Steinfurth, Fabian,Kropil, Feride,Krieg, Andreas,Sarikaya, Hulya,Knoefel, Wolfram Trudo,Kruger, Martin,Benhidjeb, Tahar,Beshay, Morris,Esch, Jan Schulte am The Korean Gastric Cancer Association 2019 Journal of gastric cancer Vol.19 No.4
Surgical therapy for adenocarcinoma of the esophagogastric junction II requires distal esophagectomy, in which a transhiatal management of the lower esophagus is critical. The 'dorsal track control' (DTC) maneuver presented here facilitates the atraumatic handling of the distal esophagus, in preparation for a circular-stapled esophagojejunostomy. It is based on a ventral semicircular incision in the distal esophagus, with an intact dorsal wall for traction control of the esophagus. The maneuver facilitates the proper placement of the purse-string suture, up to its tying (around the anvil), thus minimizing the manipulation of the remaining esophagus. Furthermore, the dorsally-exposed inner wall surface of the ventrally-opened esophagus serves as a guiding chute that eases anvil insertion into the esophageal lumen. We performed this novel technique in 21 cases, enabling a safe anastomosis up to 10 cm proximal to the Z-line. No anastomotic insufficiency was observed. The DTC technique improves high transhiatal esophagojejunostomy.
Tasargol Omer,Darbaz Isfendiyar,Ergene Osman,Zabitler Feride,Deniz Aziz,Kose Selin Guven,Kose Halil Cihan,Tulgar Serkan 대한마취통증의학회 2023 Korean Journal of Anesthesiology Vol.76 No.2
Background: Animal and other experimental studies have demonstrated increased block time and quality when α- and β-cyclodextrin drugs are combined with local anesthetics. However, to our knowledge, no study has utilized γ-cyclodextrins in such a combination. In the present study, we used an animal model to evaluate the effects of different doses of the combined administration of γ-cyclodextrin (sugammadex) and bupivacaine on the duration of sciatic nerve blocks in rats. Methods: Sciatic nerve blocks were performed with a 0.20 ml mixture in all groups. For the non-experimental groups, this mixture consisted of 0.2 ml saline (Sham group), 0.2 ml sugammadex (Group S), or 0.16 ml bupivacaine 0.5% and 0.04 ml saline (Group B). For the experimental groups, 0.16 ml bupivacaine 0.5% was administered along with 0.01 ml sugammadex and 0.03 ml saline (Group BS1), 0.02 ml sugammadex and 0.02 ml saline (Group BS2), or 0.04 ml sugammadex (Group BS4). Proprioception, nociception, and motor function were evaluated until the sciatic block was completely reversed.Results: Motor, proprioceptive, and nociceptive blockades occurred within 5 min in all experimental groups. In Group BS4, the duration of the motor, proprioceptive, and nociceptive blockades was significantly increased compared with the other experimental groups. However, in Groups BS1 and BS2, only the duration of the nociceptive blockade was significantly increased.Conclusions: The combined administration of sugammadex and bupivacaine for sciatic nerve blocks in rats led to a significant increase in the duration of motor, proprioceptive, and nociceptive blockades.
Mahria Hammami,Dorra Jellouli Ennigrou,Karima Horchani-Naifer,Mokhtar Ferid 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.2
The Polyelectrolyte-Assisted UltraFiltration technique has demonstrated its effectiveness in the recovery of neodymium from aqueous solutions. In this study, poly(sodium 4-styrenesulfonate) and polyethylene glycol were selected as the complexing agents. The ultrafiltration experiments were operated using a laboratory scale ultrafiltration system equipped with a polyethersulfone membrane. The effects of various parameters such as polyelectrolyte concentration, applied pressure, NaCl concentration, pH and operating time on neodymium retention and permeation flux were studied. It was observed that the better result was obtained with PSS. The maximum retention was observed at 2×10−4 mol L−1 and 3 bars for both polyelectrolytes. The results showed that the retention increased with the increase of pH. The ionic strength effect has shown a retention decrease with the increasing of salt concentration. Finally, the permeate flux declined following the increment in volume concentration factor, the retention remained constant around 98%.