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Tamires Carvalho dos Santos,Nadabe dos Santos Reis,Tatielle Pereira Silva,Fabiano de Paula Pereira Machado,Renata Cristina Ferereira Bonomo,Marcelo Franco 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1
Prickly palm cactus husk was used as a solid-state fermentation support-substrate for production of the ligninolytic enzymes laccase, peroxide manganese, and lignin peroxidase by Aspergillus niger. Effects of water activity, temperature, and fermentation time on enzymatic production were evaluated using a central composite rotatable design. Response surface methodology revealed that maximum enzyme production was achieved at 73.38 h of fermentation, a water activity of 0.87 Aw, at 28.74oC for laccase, at 65.33 h, 0.89 Aw, and 28.96oC for lignin peroxidase, and at 70.44 h, 0.91 Aw, and 28.84oC for manganese peroxidase. Optimized enzyme production was 9,023.67 UI/L for laccase, 2,234.75 UI/L for lignin peroxidase, and 8,534.81 UI/L for manganese peroxidase. Thermostability and pH stability were observed for all enzymes. Enzymatic deactivation kinetic experiments indicated that enzymes remained active after freezing of crude extracts.