Molecular and Cellular Microbiology / Biomedical Sciences : A Homogeneous Immunoassay Method for Detecting Interferon-Gamma in Patients with Latent Tuberculosis Infection

( Fei Wu ),( Lin Wang ),( Qiaomei Guo ),( Mingna Zhao ),( Hongchen Gu ),( Hong Xu ),( Jiatao Lou ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3

IFN-γ release assays (IGRAs) have been developed as viable alternative diagnostic tools for detecting latent tuberculosis infection (LTBI). A customized homogeneous sandwich luminescent oxygen channeling immunoassay (LOCI) was used to quantify IFN-γ levels in IGRAs. Samples were collected from healthy volunteers (n = 40) who were T-Spot-negative and T-Spot-positive patients (n = 32) at rest. Then the amount of IFN-γ in the supernatant of IGRAs was measured by LOCI. The results demonstrated a low background, and high sensitivity, specificity, accuracy, and reproducibility, and a short assay time (only 30 min) with LOCI for IFN-γ. The recovery range was 81.63-102.06%, the coefficients of variation were below 5%, and the limit of detection was 19.0 mIU/ml. Excellent agreement between LOCI IFN-γ and the T-SPOT.TB test was obtained (97.2% agreement, κ = 0.94). The LOCI IFN-γ concentrations were significantly higher in T-Spot-positive patients than in the healthy group (p < 0.001). Moreover, as observed for the comparative LOCI IFN-γ assay, IFN-γ concentrations were related to the numbers of T-SPOT.TB spots. We have established an in vitro blood test for LTBI diagnosis, defined as LOCI IFN-γ. A high level of agreement between the LOCI IFN-γ method and T-SPOT.TB assay was observed in clinical studies that showed the LOCI IFN-γ method could determine LTBI. This study shows acceptable performance characteristics of the LOCI IFN-γ assay to diagnose LTBI.

Concentrated Trading and Order Execution Costs

Fei Wu,Ryan Garvey,Tao Huang 한국증권학회 2014 Asia-Pacific Journal of Financial Studies Vol.43 No.6

Prior studies indicate that investors who hold more concentrated investment portfolios per-form better. In our paper, we examine whether concentration benefits occur in trading set-tings. We find that US traders who focus on fewer stocks pay lower order execution costs. Concentrated trading behavior is correlated across different dimensions. Traders who concen-trate on a smaller number of stocks also concentrate in fewer market centers, at particulartimes of the day, and on certain order types. Our results suggest that trading costs varyacross market participants, and that by focusing on a smaller subset of securities, traders gainadvantages for order execution.

Do Stock Prices Underreact to Information Conveyed by Investors’ Trades? Evidence from China

Fei Wu 한국증권학회 2013 Asia-Pacific Journal of Financial Studies Vol.42 No.3

We examine the process of stock prices adjusting to information conveyed by the trading process. Using the price impact of a trade to measure its information content, we show that the weekly price impact has significant cross-sectional predictive power for returns in the subsequent week. The effect is sensitive to the level of informational asymmetry. We find that the price impact contains information that is not fully captured by public order flows and that a lead–lag effect exists regarding the arrival of information to different groups of investors. Our finding suggests a price under-reaction to trading information, which can be explained by the gradual-information-diffusion theory.

Effects of Matching Conditions Between Crocheted Mesh Tube and Metal Tube on Crushing Behaviour and Energy Absorption of Interlayer Tubes

Fei Wu,Xiaoting Xiao,Qian Liu,Jianghuai Tong 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.4

This paper focuses on comparing the quasi-static crushing mechanical behaviour and energy absorption characteristics of four types of interlayer tubes formed by combining mesh tube and 304 tube with two diff erent interface matching methods (tight fi t and metallurgical bonding). The deformations and energy absorptions of interlayer tubes are still dominated by 304 tube. The mesh tube and metallurgical points are able to induce and improve the deformation of 304 tube by changing its axial crushing stress state, thus, aff ecting the shapes and quantities of its folds, changing its deformation behaviours and modes, and fi nally forming interlayer tubes with diff erent mechanical behaviours and energy absorption characteristics. The tight fi t interlayer tubes exhibit better mechanical behaviour and energy absorption compared with the hollow 304 tube. The sintering interlayer tubes matching by metallurgical bonding can further improve the mechanical behaviours and energy absorptions of interlayer tubes and present more stable crushing deformations and stronger bearing capacities. It is an eff ective method to improve the mechanical behaviours and energy absorption characteristics of interlayer tubes by changing interface matching modes.

Network Traffic Identification Algorithm Based on Neural Network

Fei Wu,Yong Ye,Hongfa Li,Shilong Ni,Jiangwen Su 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.12

In this paper, a network traffic identification model is established using a multilayer excitation function quantum neural network which is suitable for data classification. Because the conventional quantum neural network has different target function in the training of the weights of the network and the sigmoid function of the neurons in the hidden layer, the coupling effect of the two parameters is not processed. This will result in the middle and later stage of the training iteration process, and it may be possible to reduce the objective function value of a kind of parameter, and make the objective function value of another kind of parameter increase. In order to avoid this situation, using LM algorithm to optimize, using the same objective function not only as the target function of the network weight, but also the function of translational spacing of sigmoid function of neurons in the hidden layer, and the training objective is to minimize the sum of squared error of the neural network output and the desired value. Finally, the recognition performance of the proposed algorithm is compared with that of the conventional quantum neural network and LM-BP neural network. The results show that the convergence rate of the proposed algorithm is the fastest and the convergence accuracy is the highest.

Wave propagation in double nano-beams in thermal environments using the Reddy's high-order shear deformation theory

Fei Wu,Gui-Lin She Techno-Press 2023 Advances in nano research Vol.14 No.6

We study the bending wave, shear wave and longitudinal wave characteristics in the double nanobeams in this paper for the first time, in the process of research, based on the Reddy's higher-order shear deformation theory and considering shear layer stiffness, linear stiffness, inter-laminar stiffness, the pore volume fraction, temperature variation, functionally graded index influence on wave propagation, based on the nonlocal strain gradient theory and Hamilton variational principle, the wave equation of the double-nanometer beams are derived. Since there are three different motion states for the double nanobeams, which includes the cases of "in phase", "out of phase" and "one nanobeam fixed", the propagation characteristics of shear-, bending-, and longitudinal- waves in these three cases are discussed respectively, and some valuable conclusions are obtained.

Three New Loci of Insertion Element IS1112 in Chinese Strains of Xanthomonas oryzae pv. Oryzae

Jiajian Xie,Xifeng Wang,Feiwu Li,Yufa Peng,Guanghe Zhou 한국미생물학회 2007 The journal of microbiology Vol.45 No.3

Insertion sequence IS1112 is a repetitive element with a relatively high number of copies in Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice (Oryza sativa L.). Three new loci of IS1112 were identified in seven Chinese strains of Xoo using a single oligonucleotide primer J3; 5'-GCTCAGGTCAGGTGGCCTGG-3’by insertion-sequence-based polymerase chain reaction (IS-PCR). Among the three new loci of IS1112, two were located in the open-reading frame region of genes fhuA and cirA, which encode TonB-dependent receptors, and the third in ISXo2, another type of insertion sequence in Xoo genome. Three variants of IS1112 were identified in those three loci based on their sequence similarities: two were identical to IS1112a and IS1112b, reported in strain PXO86 from the Philippines, while the third was a new member of IS1112, defined as IS1112d. Inserting IS1112 in gene fhuA caused three bases, GGT, to be duplicated at the target site, but inserting it in gene cirA did not cause any duplication in the target site. The diversity of IS1112 sequence and insertion loci in Xoo genome and their potential effects are discussed.

Three New Loci of Insertion Element IS1112 in Chinese Strains of Xanthomonas oryzae pv. oryzae

Xie, Jiajian,Wang, Xifeng,Li, Feiwu,Peng, Yufa,Zhou, Guanghe The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.3

Insertion sequence IS1112 is a repetitive element with a relatively high number of copies in Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice (Oryza sativa L.). Three new loci of IS1112 were identified in seven Chinese strains of Xoo using a single oligonucleotide primer J3; 5'-GCTCA GGTCAGGTGGCCTGG-3' by insertion-sequence-based polymerase chain reaction (IS-PCR). Among the three new loci of IS1112, two were located in the open-reading frame region of genes fhuA and cirA, which encode TonB-dependent receptors, and the third in ISXo2, another type of insertion sequence in Xoo genome. Three variants of IS1112 were identified in those three loci based on their sequence similarities: two were identical to IS1112a and IS1112b, reported in strain PXO86 from the Philippines, while the third was a new member of IS1112, defined as IS1112d. Inserting IS1112 in gene fhuA caused three bases, GGT, to be duplicated at the target site, but inserting it in gene cirA did not cause any duplication in the target site. The diversity of IS1112 sequence and insertion loci in Xoo genome and their potential effects are discussed.