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Fazle Elahi,Sang-Hwan Hyun,EunsongLee 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
Mitochondrial dysfunction is found in oocytes and transmitted to the offspring due to maternal obesity. This is curable by endoplasmic reticulum (ER) stress inhibitors such as salubrinal (SAL). Recently pigs are considered as a model animal for biomedical research due to its physiological similarity with human. Pig oocytes have shown ER stress mostly in metaphase II stage. ER stress is hindering the in vitro embryo production (IVP). This study investigated the effect of ER stress inhibition by using SAL during 44 h of in vitro maturation (IVM) of oocytes at 1, 10, 50 and 100 nM concentrations. Firstly, we defined the concentration of SAL during IVM of pig oocytes. SAL at 10 nM showed higher (44.2 to 55.6%, P<P0.05) development competence to the blastocyst state than control and other concentrations after parthenogenetic activation (PA). Secondly, we sorted out the time-dependent treatment at 10 nM of SAL for IVM of oocytes. It revealed that treatment with SAL during 22 to 44 h and 0 to 44 h of IVM improved PA embryonic development significantly (40.5, 51.7 and 60.2% for control, 22 to 44 h and 0 to 44 h of IVM, respectively, P<0.05). Glutathione (GSH) level is an indicator of cytoplasmic maturation of oocytes. Reactive oxygen species (ROS) have a harmful effect on development competence of oocytes. For this, we determined the intraoocyte levels of GSH and ROS after 44 h of IVM. It was found that SAL increased intraoocyte GSH level and also decrease ROS level (P<0.05). Finally, we performed somatic cell nuclear transfer (SCNT) after treating oocytes with 10 nM SAL during IVM. SAL treatment significantly improved blastocyst formation of SCNT embryos compared to control (24.7 vs. 39.6%, P<0.05). Our results indicate that treatment of pig oocytes with ER stress inhibitor SAL during IVM improves preimplantation development cloned pig embryos by influencing cytoplasmic maturation in terms of increased GSH content and decreased ROS level in IVM pig oocytes.
( Fazle Rabbi Chowdhury ),( Mohammed Zakiul Islam ),( Ralf Weige,Nazia Hassan ),( Ismail Patwary ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: In Bangladesh there are around 300,000 new cases of TB every year with 70,000 deaths. In spite of major national and global efforts obstacles to effective diagnosis and treatment still persist. This study sets out to assess factors like socio- economic and demographic characteristics, patients care seeking pattern, average cost etc.in north eastern part of Bangladesh. Methods: This cross sectional study was done by interviewing systematically selected patients in two urban and two semi-urban DOTS center from July 2012 to June 2013 using a structured questionnaire considering socio-economic status, care seeking pattern, money spent for diagnosis & treatment and quality of service etc. Sputum smear positive patients aged = 19 years receiving treatment for = four weeks were enrolled. Chi-square test was done to explore the level of signifi cance. Results: Out of 170 patient`s majority (76; 44.7%) was belonged to most economically productive age group (25-44 years). About one third (59; 34.7%) patients were the only earning member of the family and majority (131; 77.05%) of them was not working due to current illness (p = 0.005). Delay in diagnosis (117; 68.82%) was common ((p = 0.005) among semi-urban patients while they (121; 71.1%) also experienced signifi - cant (p = 0.005) delay (> 3 weeks) to reach the DOTS corner and spent more money (> 15 USD) to confi rm their diagnosis compared to urban counterpart (p = 0.005). 70% patients utilized private health care services initially and fear of government formalities (87; 73.1%) was the most common reason for that. Total 27.05% participants suspect about quality of drug. Conclusions: The linkage between TB and poor socio economic condition is well documented. Policy makers should implement “pro-poor bias” in their TB strategies. Regular monitoring is essential to overcome the barriers.
Mental Workload and Task Engagement Evaluation Based on Changes in Electroencephalogram
Ahmed Fazle Rabbi,Reza Fazel-Rezai,Abongwa Zony,,Pablo de Leon 대한의용생체공학회 2012 Biomedical Engineering Letters (BMEL) Vol.2 No.3
Purpose In this study, electroencephalogram (EEG) signals were used to assess subject’s mental workload and task engagement level during a set of neurocognitive tasks in an experimental space suit. Methods EEG signals were collected using a wireless EEG system during two experimental conditions – when subjects did/did not wear space suit. Brain state changes based on EEG changes were quantified and compared with the direct responses of the subjects for different tasks. In addition, a statistical test of significance on the computed EEG index for the two experimental conditions was performed. Results It was found that the spacesuit experiment introduced a greater mental workload where subject's stress levels were higher than control experiment. Results indicated significant differences in task engagement between the spacesuit and control experiments for most of the tasks. Conclusions The findings could be useful in monitoring astronaut’s or human subject’s cognitive performance in assuring safety as well as improving the performance.
Elahi, Fazle,Shin, Hyeji,Lee, Joohyeong,Lee, Seung Tae,Lee, Geun-Shik,Lee, Eunsong The Korean Society of Embryo Transfer 2018 한국동물생명공학회지 Vol.33 No.4
Nitric oxide (NO) has an important role in oocyte maturation and embryonic development in mammals. This study examined the effect of exogenous NO donor S-nitroso-N-acetylpenicillamine (SNAP) in a maturation medium on meiotic progression and embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) in pigs. When oocytes were exposed to $0.1{\mu}M$ SNAP for first 22 h of in vitro maturation (IVM) in Experiment 1, SNAP significantly improved blastocyst development in both defined and standard follicular fluid-supplemented media compared to untreated control (48.4 vs. 31.7-42.5%). SNAP treatment significantly arrested meiotic progression of oocytes at the germinal vesicle stage at 11 h of IVM (61.2 vs. 38.7%). However, there was no effect on meiotic progression at 22 h of IVM (Experiment 2). In Experiment 3, when oocytes were treated with SNAP at 0.001, 0.1 and $10{\mu}M$ during the first 22 h of IVM to determine a suitable concentration, $0.1{\mu}M$ SNAP (54.2%) exhibited a higher blastocyst formation than 0 and $10{\mu}M$ SNAP (36.6 and 36.6%, respectively). Time-dependent effect of SNAP treatment was evaluated in Experiment 4. It was observed that SNAP treatment for the first 22 h of IVM significantly increased blastocyst formation compared to no treatment (57.1% vs. 46.2%). Antioxidant effect of SNAP was compared with that of cysteine. SNAP treatment significantly improved embryonic development to the blastocyst stage (49.1-51.5% vs. 34.4-37.5%) irrespective of the presence or absence of cysteine (Experiment 5). Moreover, SNAP significantly increased glutathione (GSH) content and inversely decreased the reactive oxygen species (ROS) level and mitochondrial oxidative activity in IVM oocytes. SNAP treatment during IVM showed a stimulating effect on in vitro development of SCNT embryos (Experiment 7). These results demonstrates that SNAP improves developmental competence of PA and SCNT embryos probably by maintaining the redox homeostasis through increasing GSH content and mitochondrial quality and decreasing ROS in IVM oocytes.
Elahi, Fazle,Shin, Hyeji,Lee, Joohyeong,Lee, Eunsong The Korean Society of Embryo Transfer 2017 한국동물생명공학회지 Vol.32 No.4
Mitochondrial dysfunction is found in oocytes and transmitted to offspring due to maternal obesity. Treatment of obese mothers with endoplasmic reticulum (ER) stress inhibitors such as salubrinal (SAL) can reverse the mitochondrial dysfunction and result in normal embryonic development. Pig oocytes have also shown ER stress mostly in metaphase II stage. ER stress in oocytes may hinder the in vitro production of pig embryos. This study investigated the effect of ER stress inhibition by SAL treatment during in vitro maturation (IVM) of porcine oocytes at 1, 10, 50 and 100 nM concentrations. Firstly, we tested various concentrations of SAL. SAL at 10 nM showed higher (P < 0.05) developmental competence to the blastocyst stage (55.6%) after parthenogenesis (PA) than control (44.2%) while not different from other concentrations (49.2, 51.6, and 50.8% for 1, 50, and 100 nM, respectively). Secondly, we performed time-dependent treatment at 10 nM of SAL for IVM of oocytes. It revealed that treatment with SAL during 22 to 44 h of IVM significantly improved PA embryonic development to the blastocyst stage compared to control (40.5, 46.3, 51.7 and 60.2% for control, 0 to 22 h, 22 to 44 h and 0 to 44 h of IVM, respectively, P < 0.05). Glutathione (GSH) content is an indicator of cytoplasmic maturation of oocytes. Reactive oxygen species (ROS) have a harmful effect on developmental competence of oocytes. For this, we determined the intraoocyte levels of GSH and ROS after 44 h of IVM. It was found that SAL increased intraoocyte GSH level and also decreased ROS level (P < 0.05). Finally, we performed somatic cell nuclear transfer (SCNT) after treating oocytes with 10 nM SAL during IVM. SAL treatment significantly improved blastocyst formation of SCNT embryos compared to control (39.6% vs. 24.7%, P < 0.05). Our results indicate that treatment of pig oocytes with ER stress inhibitor SAL during IVM improves preimplantation development PA and cloned pig embryos by influencing cytoplasmic maturation in terms of increased GSH content and decreased ROS level in IVM pig oocytes.
Handwritten Signature Verification Using CNN with Data Augmentation
Md. Tariqulhasan Fazle Rabbi,S. M. Tanjilur Rahman,Prokash Biswash,Jinsul Kim,Alamin Sheikh,Aloke Kumar Saha,Mohammad Shorif Uddin 한국디지털콘텐츠학회 2019 The Journal of Contents Computing Vol.1 No.1
A signature is a mark or sign which is made by an individual on an instrument or document to signify knowledge, approval, acceptance or obligation. To authenticate writing or a notice of its source and to bind the individual signing which is written by the provisions contained in the document. Signature verification is more important for not only in commercial banks but also with every sector like falsification of documents in numerous financial, legal and other commercial aspects. A signature is an important factor in biometric technique in which it is used to detect forged or genuine signature. This paper concerns offline handwritten signature verification using convolutional neural network (CNN). Here we have used data augmentation with CNN model and also, we have made a comparative study with Multilayer Perceptron (MLP) and Single Layer Perceptron (SLP). The model is tested using 4480 images with 20 subjects where we have found the accuracy of CNN is 82.75% and CNN with data augmentation is 98.33%, SLP is 39.91% and MLP is 63.57%. Based on the comparative study CNN with data augmentation proves the best performance.