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Ahmad, Nazir,Ahmed, Iftikhar,Shahzad, Armghan,Khalid, Nauman,Mehboob, Farrakh,Ahad, Karam,Ali, Ghulam Muhammad The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.3
Phenol is a toxic pollutant found in effluent of numerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collected from industrial waste and enriched in mineral salt medium (MSM) contained 300 mg/L phenol. The strain was identified based on 16S rRNA gene analysis as Pseudomonas species and the phylogenetic analysis affiliated the strain with Pseudomonas monteilii (AF064458) as the most closely related species. Phenol tolerance of the strain in MSM supplemented with various concentrations of phenol indicates that the strain NCCP-407 can grow best at $750mgL^{-1}$ phenol. The strain showed complete degradation of $750mgL^{-1}$ phenol in 56 hours when supplement as a sole source of carbon and energy with the average degradation rate of $28mgL^{-1}h^{-1}$. The doubling time was recorded approximately as $12.49h^{-1}$. The present study suggests that this strain is efficient in phenol degradation and can be used in treatment of wastewater containing phenol.
Nazir Ahmad,Iftikhar Ahmed,Armghan Shahzad,Nauman Khalid,Farrakh Mehboob,Karam Ahad,Ghulam Muhammad Ali 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.3
Phenol is a toxic pollutant found in effluent ofnumerous industries and its elimination is a foremost challenge. The utilization of bacteria plays a crucial role in phenol bioremediation. For isolation of phenol degrading bacteria, sample was collectedfrom industrial waste and enriched in mineral salt medium (MSM)contained 300 mg/L phenol. The strain was identified based on16S rRNA gene analysis as Pseudomonas species and thephylogenetic analysis affiliated the strain with Pseudomonasmonteilii (AF064458) as the most closely related species. Phenoltolerance of the strain in MSM supplemented with variousconcentrations of phenol indicates that the strain NCCP-407 cangrow best at 750 mg L−1 phenol. The strain showed completedegradation of 750 mg L−1 phenol in 56 hours when supplementas a sole source of carbon and energy with the average degradationrate of 28mg L−1 h−1. The doubling time was recorded approximatelyas 12.49 h−1. The present study suggests that this strain is efficientin phenol degradation and can be used in treatment of wastewatercontaining phenol.
Niazi, Nabeel Khan,Bibi, Irshad,Shahid, Muhammad,Ok, Yong Sik,Shaheen, Sabry M.,Rinklebe, Jö,rg,Wang, Hailong,Murtaza, Behzad,Islam, Ejazul,Farrakh Nawaz, M.,Lü,ttge, Andreas Elsevier 2018 Science of the Total Environment Vol.621 No.-
<P><B>Abstract</B></P> <P>In this study, we examined the sorption of arsenite (As(III)) and arsenate (As(V)) to Japanese oak wood-derived biochar (OW-BC) in aqueous solutions, and determined its efficiency to remove As from As-contaminated well water. Results revealed that, among the four sorption isotherm models, Langmuir model showed the best fit to describe As(III) and As(V) sorption on OW-BC, with slightly greater sorption affinity for As(V) compared to As(III) (<I>Q<SUB>L</SUB> </I> =3.89 and 3.16mgg<SUP>−1</SUP>; R<SUP>2</SUP> =0.91 and 0.85, respectively). Sorption edge experiments indicated that the maximum As removal was 81% and 84% for As(III)- and As(V)-OW-BC systems at pH7 and 6, respectively, which decreased above these pH values (76–69% and 80–58%). Surface functional groups, notably OH, COOH, CO, CH<SUB>3</SUB>, were involved in As sequestration by OW-BC, suggesting the surface complexation/precipitation and/or electrostatic interaction of As on OW-BC surface. Arsenic K-edge X-ray absorption near edge structure (XANES) spectroscopy indicated that 36% of the added As(III) was partially oxidized to As(V) in the As(III) sorption experiment, and in As(V) sorption experiment, 48% of As(V) was, albeit incompletely, reduced to As(III) on OW-BC surface. Application of OW-BC to As-contaminated well water (As: 27–144μgL<SUP>−1</SUP>; <I>n</I> =10) displayed that 92 to 100% of As was depleted despite in the presence of co-occurring competing anions (e.g., SO<SUB>4</SUB> <SUP>2−</SUP>, CO<SUB>3</SUB> <SUP>2−</SUP>, PO<SUB>4</SUB> <SUP>3−</SUP>). This study shows that OW-BC has a great potential to remove As from solution and drinking (well) water. Overall, the combination of macroscopic sorption data and integrated spectroscopic and microscopic techniques highlight that the fate of As on biochar involves complex redox transformation and association with surface functional moieties in aquatic systems, thereby providing crucial information required for implication of biochar in environmental remediation programs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Arsenic removal efficiency of Japanese oak wood biochar (OW-BC) was explored. </LI> <LI> Langmuir model provided the best fit, with a greater <I>Q<SUB>L</SUB> </I> for arsenate than arsenite. </LI> <LI> XANES spectroscopy indicated redox transformation of arsenite⇔arsenate on OW-BC. </LI> <LI> FTIR spectra revealed arsenite/arsenate association with functional groups on OW-BC. </LI> <LI> OW-BC efficiently removed As (92 to 100%) from drinking well water. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>