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A. Evangeline,P. Sathiya 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.5
The solid solution strengthened Inconel 625, a Ni-based alloy is known for its excellent strength and good corrosion resistanceat extreme environments used in thermal plants, boiler tubes, petrochemical industry and power plant. The presence ofCr content (~ 20 wt%) along with Mo-rich, Nb and Fe makes Ni–Cr–Mo–Nb austenitic alloy called as Inconel 625 to achieveexcellent corrosion resistance property. Using cold metal arc transfer (CMT) cladding, the metallurgical, mechanical andcorrosion properties of Inconel 625 on 316L is evaluated. The process parameters selected includes welding current, torchangle and travel speed with a constant voltage. From the results of microstructural and EDS inferences, the formation of cellulardendritic structure with secondary phases like Laves phase, complex nitrides along with the interdendritic segregationof Mo and Nb as well as microsegregation of Cr, Ni and Fe. In case of Ni–Cr–Mo alloy, Ni and Cr contribute to resistance tocorrosion in NaCl environments. The formation of Cr2O3and the passivation action of the clad zone is due to the presence ofCr. The solid solution effect in Ni–Cr matrix is contributed by the presence of Nb and Mo. Apart from that the strengtheningaction happens due to the precipitation of Ni3(Al, Ti, Nb) commonly known as γ′, γ″ and MC carbides confirmed throughXRD. Uni-axial tensile tests and Vickers-micro hardness indentation tests were performed on Inconel 625 cladded over316L. Based on the fractographic results fatigue striations, tear rigdes with river markings, dimples with fibrous structureand cleavages are observed. Unlike other studies, unique type of cuboidal precipitates are seen, which is due to the presenceof Ti, which form carbonitrides containing Ti, which are further characterised as NbC. The potentiodynamic polarisationtests is performed on 3.5% NaCl solution. The results suggest that Ni–Cr–Mo alloy protects the substrate from corrosion.
Effect of microorganism on engineering properties of cohesive soils
Yasodian, Sheela Evangeline,Dutta, Rakesh Kumar,Mathew, Lea,Anima, T.M.,Seena, S.B. Techno-Press 2012 Geomechanics & engineering Vol.4 No.2
This paper presents the study of the effect of microorganism Bacillus pasteurii on the properties such as Atterbergs' limit and unconfined compressive strength of cohesive soils. The results of this study reveal that the liquid limit and plasticity index for all clay soils decreased and the unconfined compressive strength increased. Decrease in plasticity index is very high for Kuttanad clay followed by bentonite and laterite. The unconfined compressive strength increased for all the soils. The increase was high for Kuttanad soil and low for laterite soil. After 24 h of treatment the improvement in the soil properties is comparatively less. Besides the specific bacteria selected Bacillus pasteurii, other microorganisms may also be taking part in calcite precipitation thereby causing soil cementation. But the naturally present microorganisms alone cannot work on the calcite precipitation.
Acebedo, Alvin Resultay,Amor, Evangeline Cancio,Jacinto, Sonia Donaldo Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2
Voacanga globosa (Blanco), a plant endemic to the Philippines, is traditionally used especially by indigenous people of Bataan in the treatment of ulcers, wounds and tumorous growths. This study aimed to provide scientific evidence to therapeutic properties by determining cytotoxic and pro-apoptotic activity of HPLC fractions from leaves on HCT116 human colon carcinoma and A549 human lung carcinoma cell lines. Ethanolic extraction was performed on V globosa leaves followed by hexane and ethyl acetate partitioning. Silica gel column chromatography and high performance liquid chromatography (HPLC) produced MP1, MP2 and MP3 fractions. Cytotoxic activity of the fractions was determined through MTT assay against the cancer cell lines HCT116 and A549 and the non-cancer AA8 Chinese hamster ovarian cell line. Pro-apoptotic activities of the most active fractions were further assessed through DAPI staining, TUNEL assay and JC-1 mitochondrial membrane potential assay with HCT116 cells. While the MPI fraction exerted no significant activity against all cell lines tested, MP2 and MP3 fractions demonstrated high toxicity against HCT116 and A549 cells. The MP3 fraction induced formation of apoptotic bodies, condensed DNA and other morphological changes consistent with apoptosis of HCT116 cells and TUNEL assay showed significant increase in DNA fragmentation over time. In these cells, the MP3 fraction also induced mitochondrial membrane destabilization, which is generally associated with the beginning of apoptosis. Phytochemical analysis demonstrated the presence only of saponins and terpenoids in the MP3 fraction. The results indicate that the MP3 fraction exerts cytotoxic activity on HCT116 cells via induction of apoptosis triggered by loss of mitochondrial membrane potential crucial for cell survival.
Shine Let Gunamony,Josemin Bala Gnanadhas,Diana Evangeline Lawrence 한국전자파학회JEES 2020 Journal of Electromagnetic Engineering and Science Vol.20 No.3
Presently, a single compact antenna is expected to operate in multiple frequency bands, so that it may be used for multiple applications. In this regard, a compact asymmetric coplanar strip (ACS) fed monopole antenna was designed to operate in LTE band-40 and 5G mid band frequencies. To achieve the desired dual band frequency, meander line radiating structure was used. The uniplanar design with the ACS feed considerably reduced the antenna size to 19.25 × 10.5 × 1.6 ㎟. This miniaturized dual band antenna can be easily integrated into circuit boards. The measured and simulated results provided a reflection coefficient (S11) < –15 dB, which made the antenna suitable for LTE band-40 and 5G mid-band communication applications.
Ko, Hyeonseok,Kim, Young‐,Joo,Amor, Evangeline C.,Lee, Jong Wha,Kim, Han‐,Cheon,Kim, Hee Ju,Yang, Hyun Ok Wiley Subscription Services, Inc., A Wiley Company 2011 Journal of cellular biochemistry Vol.112 No.9
<P><B>Abstract</B></P><P>Dimethyl cardamonin (2′,4′‐dihydroxy‐6′‐methoxy‐3′,5′‐dimethylchalcone; DMC) is a naturally occurring chalcone, and it is the major compound isolated from the leaves of <I>Syzygium samarangense</I> (Blume) Merr. & L.M. Perry (Myrtaceae). Experiments were conducted to determine the effects of DMC on cell proliferation, cell‐cycle distribution, and programmed cell death in cultures of human colorectal carcinoma HCT116 and LOVO cells. Results showed that DMC inhibited HCT116 and LOVO cell proliferation and induced G<SUB>2</SUB>/M cell cycle arrest, which was associated with the conversion of microtubule associated protein light chain 3 (LC3)‐I–LC3‐II, an autophagosome marker, and the incorporation of monodansylcadaverine (MDC), a marker for the acidic compartment of autolysosomes or acidic vesicular organelles. The treatment of HCT116 and LOVO cells using a combination of DMC with an autophagy inhibitor, such as 3‐methyladenine (3‐MA), beclin 1 siRNA, or atg5 siRNA, suppressed the effect of DMC‐mediated anti‐proliferation. These results imply that DMC can suppress colorectal carcinoma HCT116 and LOVO cell proliferation through a G<SUB>2</SUB>/M phase cell‐cycle delay, and can induce autophagy, the hallmark of Type II programmed cell death (PCD). Taken together, our results suggest that DMC may be an effective chemotherapeutic agent for HCT116 and LOVO colorectal carcinoma cells. J. Cell. Biochem. 112: 2471–2479, 2011. © 2011 Wiley‐Liss, Inc.</P>
Injuries and Injury Management among DanceSport Competitors in Western Visayas, Philippines
Teresita E. Portugalete,Cherry Pilapil Añ asco,Evangeline B. Ybanez 상명대학교 글로벌문화예술교육연구소 2023 Research in Dance and Physical Education Vol.7 No.3
This research assessed the injuries encountered and injury management techniques employed by DanceSport competitors in Western Visayas region of the Philippines who had been competing for at least three years both locally and internationally. A checklist of common injuries and injury management techniques were formulated and validated by three experts. Injuries in different regions of the DanceSport competitors’ body were measured using a 3-point Likert Scale based on the experience of the competitor. A total of 25 Modern Standard and 25 Latin-American competitors were selected using a purposive sampling method. Descriptive statistics was used to summarize the injuries encountered and the corresponding management techniques employed by the competitors. Moreover, Mann Whitney U test was utilized to see significant differences between Modern Standard and Latin-American competitors with respect to frequency of injuries encountered and management technique, and the relationship between injury and injury management was determined using 0.05 level of significance. Results revealed that DanceSport competitors encountered stiff neck, herniated disc at cervical, hamstring muscle injury, and posterior tibial tendonitis injuries. During training and prior to actual competition, warming up before doing an activity, staying fit and exercising to keep the muscles stronger and more resistant to injury were the management techniques employed. Taking relative rest, maintaining the position that provides comfort and cooling down after the activity were also practiced. No significant difference in the injury management techniques among the competitors of the two DanceSport categories. However, injuries in the shoulder region encountered among the Latin- American competitors is significantly higher than Modern Standard counterparts (U = 226.0; p = 0.04). No significant relationship was noted between injuries encountered and injury management techniques. This study showed that the injuries encountered by both DanceSport competitors are similar. It is recommended that competitors assume self-responsibility and trainers to regularly upgrade their competencies to mitigate potential injuries due this highly strenuous activity.
Lee, Kyung Joon,Lee, Kyu Hwa,Tamolang-Castillo, Evangeline,Budi, Sri Wilarso Korean Society of Forest Science 2009 한국산림과학회지 Vol.98 No.5
This study was conducted to investigate the arbuscular mycorrhizal fungal biodiversity, spore density and root colonization in relation to site ages at expressway cut-slopes in Korea. Stabilization of exposed surface involved soil amendments and spraying seed mixture of turf grasses and/or nitrogen-fixing shrub species. Eighteen sites were selected with varying ages (2 to 16 years). Soil samples collected in October from each site were analyzed for fungal diversity and spore counts. Fine root samples from the plants were assayed for fungal colonization. Of the total 37 plants inspected in the sites, 26 species had endomycorrhizal colonization with an average root colonization rate of 18%, and with a range from 1 to 67%. The average endomycorrhizal colonization rate of initially introduced Festuca arundinacea which became the most dominant grass in later stage showed 22.8%, while that of Lespedeza bicolor which became the most dominant woody species were 21.6%. Naturally-invading Robinia pseudoacacia showed higher colonization rate in the old sites. Although site age did not show significant effects on fungal diversity, the root colonization rates of initially introduced plants decreased with the site aging, while those of invading plants increased with aging of the sites. The soil chemical properties, pH, N, and P contents, were negatively correlated with spore density, root colonization and endomycorrhizal species diversity. A total of forty arbuscular mycorrhizal fungal species in seven genera were identified. Of the 40 species, Acaulospora lacunosa, Glomus aggregatum, Glomus constrictum, Scutellospora erythropa, and Acaulospora spinosa were the five most dominant species in the decreasing order.
Kim, Young‐,Joo,Kim, Han‐,Cheon,Ko, Hyeonseok,Amor, Evangeline C.,Lee, Jong Wha,Yang, Hyun Ok Wiley Subscription Services, Inc., A Wiley Company 2011 Journal of cellular biochemistry Vol.112 No.2
<P><B>Abstract</B></P><P>We identified a chalcone, 2′,4′‐dihydroxy‐6′‐methoxy‐3′‐methylchalcone (stercurensin), as an active compound isolated from the leaves of <I>Syzygium samarangense</I>. In the present study, the anti‐inflammatory effects and underlying mechanisms of stercurensin were examined using lipopolysaccharide (LPS)‐stimulated RAW264.7 cells and mice. To determine the effects of stercurensin in vitro, inducible nitric oxide synthase (iNOS) and cyclooxygenase‐2 (COX‐2) expression were analyzed by RT‐PCR and immunoblotting. Nuclear factor‐κB (NF‐κB) activation and its upstream signaling cascades were also investigated using a dual‐luciferase reporter assay, electrophoretic mobility shift assay, immunoblotting, immunofluorescence, and immunoprecipitation. To verify the effects of stercurensin in vivo, the mRNA expression levels of iNOS and COX‐2 were evaluated in isolated mouse peritoneal macrophages by quantitative real‐time PCR, and the production of tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6), and IL‐1β were assessed in serum samples from mice using a Luminex system. Pretreatment with stercurensin reduced LPS‐induced iNOS and COX‐2 expression, thereby inhibiting nitric oxide (NO) and prostaglandin E<SUB>2</SUB> production, respectively. In addition, an inhibitory effect of stercurensin on NF‐κB activation was shown by the recovery of LPS‐induced inhibitor of κB (I‐κB) degradation after blocking the transforming growth factor‐β‐activated kinase 1 (TAK1)/I‐κB kinase signaling pathway. In mouse models, stercurensin negatively regulated NF‐κB‐dependent pro‐inflammatory mediators and cytokines. These results demonstrate that stercurensin modulates NF‐κB‐dependent inflammatory pathways through the attenuation of TAK1–TAB1 complex formation. Our findings demonstrating the anti‐inflammatory effects of stercurensin in vitro and in vivo will aid in understanding the pharmacology and mode of action of stercurensin. J. Cell. Biochem. 112: 548–558, 2011. © 2010 Wiley‐Liss, Inc.</P>