경남지역에서 분리한 Salmonella Enteritidis의 항생제 감수성 검사 및 random amplification polymorphic DNA (RAPD)-PCR을 이용한 유전형 분석

김은경 ( Eun-gyeong Kim ),김민경 ( Min-kyung Kim ),권현애 ( Hyun-ae Kwon ),윤도경 ( Do-kyung Youn ),구정헌 ( Jeong-heon Koo ),박소연 ( So-yeon Park ),이희근 ( Hui-geun Lee ),조명희 ( Myeong-hui Jo ),하도윤 ( Do-yun Hah ),김철호 ( 한국동물위생학회(구 한국가축위생학회) 2018 韓國家畜衛生學會誌 Vol.41 No.3

Salmonella Enteritidis (S. Enteritidis) are found in animals, humans, and environment. In addition, S. Enteritidis draws attention to the public health concerns due to carriage of antibiotic resistance traits. For these reasons, the prevalence and antibiotic resistance patterns of S. Enteritidis are significant issues with regard to public health. To address this issues, a total of 24 strains of S. Enteritidis from 164 samples collected from several slaughterhouses in Gyeong-Nam province in order for antibiotic resistance profiles. Subsequently, we characterized the genotyping by random amplification polymorphic DNA (RAPD)-PCR. As a result, very high level of resistance to protein synthesis inhibition antibiotics and most isolates were susceptible to others. Six random primers were used for RAPD-PCR to reveal genotypes of S. Enteritidis isolates. One of the primer, P1245, generated 147 distinct RAPD-PCR fragments ranging from 400∼3000 bp. The number of RAPD-PCR products ranged from 4 to 8 for this primer. The RAPD-PCR fragments could be placed these strains into 3 subgroups and 2 classes by UPGMA cluster analysis. Interestingly, several S. Enteritidis that isolated from different slaughterhouses showed same genotype. These results showed only limited genetic variation among the isolates, those were grouped into a few different patterns of antibiotic resistance.

그린캠퍼스 지정 여부별 대학생의 환경문제 인식 차이 분석

박은아, 민경민 충북대학교 농업과학기술연구소 2022 農業科學硏究 Vol.38 No.2

This study aimed at investigate the effects of green campus designation on university students' awareness of environmental problems. The survey was collected on 295 students from the Chungbuk University and the University of Green Campus Designated(Chungbuk Health Science University, Incheon University) from October 18, 2020 to October 31, 2020. As a result, the students of Green Cam- pus had higher awareness of Green Campus System than students of Chungbuk National University, and there was a difference in the awareness of facilities, environmental education, and school operation between Chungbuk National University and Green Campus students. also, there were significant differ- ences in concern of environmental problems, awareness of environmental situations, and awareness of seriousness of environmental problems according to whether they were educated in the environment. Finally, more than half of the students answered ‘needs’ in the need for the green campus system. There- fore, as the necessity of the green campus system is high, it is necessary to develop in a more practical direction. In order to apply the Green Campus of Chungbuk National University, various environmental education is aimed and alternatives are needed to induce students' voluntary participation.

DNA 컴퓨팅을 이용한 배낭 문제 해결

김은경,이상용 公州大學校 工學硏究院 生産技術硏究所 2004 論文集 Vol.12 No.-

Though Knapsack Problem appears to be simple, it is a NP-hard problem that is not solved in polynomial time as combinational optimization problems. To solve this problem, GA(Genetic Algorithms) was used in the past. However, there war a difficulties in real experiment because the conventional method didn't reflect the precise characteristics of DNA. This paper proposes code optimization DNA-Haskell, which is a functional language to reduce the error of DNA manipulation and to expect more accurate prediction during the DNA computing process. Code optimization DNA-Haskell was applied to (0,1) Knapsack Problem; as a result, it reduced experimental errors an compared with conventional methods, and found accurate solutions more rapidly.

Carbon tetrachloride를 투여한 rat의 hepatic lipid 축적에 미치는 vitamin E의 효과에 관한 연구

박은주,이경연,이미영,이외숙,장재정,정귀은,최진희 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

The present studies were undertaken to evaluate the effect of vitamin E, CCl_4 on the change of hepatic triglyceride, hepatic cholesterol, hepatic phospholipid in male rat. The result obtained from this study were summarised as follows: 1. Hepatic phospholipid of CCl_4 treated rat was increased in proportion to CCl_4 dosage but after concomitant injection(I.P) of vitamin E and CCl_4, hepatic phospholipid was significantly decreased in comparison to that of CCl_4 alone injection. 2. There was no effect on hepatic cholesterol concentration either CCl_4 alone injection(I.P) or concomitant injecton(I.P) of vitamin E and CCl_4. 3. Hepatic triglyceride of CCl_4-treated rat was significantly increased in comparison to that of normal rat but hepatic triglyceride of rat concomitant injection of vitamin E and CCl_4 was significantly decreased in comparison to that of CCl_4 alone injection.

재활승마의 효과에 대한 고찰

조경&#55148,김은&#51221,정재&#48124,황보&#44033 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

이 연구는 승마 운동의 효과에 대한 객관적이고 구체적인 정보를 제공하기 위해 승마운동이 균형, 자세, 골밀도, 근력, 정서에 어떤 영향을 미치는지 알아보았다. 최신 동향을 살펴보기 위해 90년대 이후의 자료만을 선택하여 승마운동, 승마치료, 재활승마 등 재활 승마와 관련된 키워드를 검색하고 승마운동을 통한 효과를 알아보기 위해 균형, 안정성, 골밀도, 근력 등의 연관 검색어를 함께 검색하였다. 그 결과 지속적인 승마운동은 균형 능력 향상을 위해 효과적인 운동이며 대퇴전자부와 대퇴경부의 좌우측, L3,L4의 골밀도를 향상시키고 승마에서만 발달될 수 있는 특수한 부위를 자극한다. 또한 재활승마를 통해 인지적, 사회적인 부분에 대해 눈에 띄는 향상을 기대할 수 있다. The purpose of this study was to provide information which objective and definite effect of horseback riding about balance, posture, bone mass, muscle strength, emotion. To search the newest trend, Studies for this literature review were sought which investigated the effect of HBRT, hippotherpay, therapeutic horseback riding since 1990. These literature searches were also linked to other descriptive terms. For example, balance, posture, bone mass, muscle strength, emotion. Continuos horseback riding therapy is effective exercise to improve balance ability and bone mass of femur trochanter, femur neck, lumbar 3-4. Also, horseback riding can expect to improve recognition and sociality.

양극성 장애에서 표피성장인자의 유전적 다형성에 대한 연합연구

김수경,이규영,주은정,강웅구,안용민,김용식 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

Objectives : Epidermal growth factor (EGF) is a neurotrophic factor which regulates the intracellular signaling molecules. These molecules are also affected by mood stabilizers such as lithium and valproate. In addition, epidermal growth factor enhances neuronal survival, maturation and differentiation especially in midbrain dopaminergic neurons of which dysfimction may play a role in pathophysiology of bipolar disorders. Bipolar disorder has some genetic commonalities with schizophrenia, and several association studies of EGF have been done with schizophrenia. In this study, we tried to investigate the geneticassociation between EGF A61G polymorphism and bipolar disorder. Methods : Total of 189 patients and 347 nonnal control were included. All patients satisfied the diagnostic criteria of DSM-IV for bipolar disorder type I (BPDI, N= 146) and bipolar disorder type II (BPDII, N=43). Genomic DNA was extracted from the peripheral blood, and genotyping was performed by TaqMan™ method. Genotype and allele frequency of EGF A61G polymorphism between the patients and the control were compared by contingency chi-square test or the Fisher's exact test. Results : No association was found between EGF A61G polymorphism and susceptibility of BPDI, BPDII and bipolar disorder (all patients). Female patients with BPDII showed overexpression of AG genotype compared to that ofcontrol group (p=0.03). However, this association was not significant after correction of multiple testing. Conclusion : In conclusion, EGF A61G polymorphism has no association with susceptibility of bipolar disorder. However, the disease modifying role of EGF gene polymorphism for bipolar disorder remains to be elucidated in respect to factors such as gender difference or diagnostic subtype.

재활승마의 효과에 대한 고찰

조경희,김은정,정재민,황보각 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

이 연구는 승마 운동의 효과에 대한 객관적이고 구체적인 정보를 제공하기 위해 승마운동이 균형, 자세, 골밀도, 근력, 정서에 어떤 영향을 미치는지 알아보았다. 최신 동향을 살펴보기 위해 90년대 이후의 자료만을 선택하여 승마운동, 승마치료, 재활승마 등 재활 승마와 관련된 키워드를 검색하고 승마운동을 통한 효과를 알아보기 위해 균형, 안정성, 골밀도, 근력 등의 연관 검색어를 함께 검색하였다. 그 결과 지속적인 승마운동은 균형 능력 향상을 위해 효과적인 운동이며 대퇴전자부와 대퇴경부의 좌우측, L3,L4의 골밀도를 향상시키고 승마에서만 발달될 수 있는 특수한 부위를 자극한다. 또한 재활승마를 통해 인지적, 사회적인 부분에 대해 눈에 띄는 향상을 기대할 수 있다. The purpose of this study was to provide information which objective and definite effect of horseback riding about balance, posture, bone mass, muscle strength, emotion. To search the newest trend, Studies for this literature review were sought which investigated the effect of HBRT, hippotherpay, therapeutic horseback riding since 1990. These literature searches were also linked to other descriptive terms. For example, balance, posture, bone mass, muscle strength, emotion. Continuos horseback riding therapy is effective exercise to improve balance ability and bone mass of femur trochanter, femur neck, lumbar 3-4. Also, horseback riding can expect to improve recognition and sociality.

재래종과 개량종 돼지고기의 영양성분 비교 연구

한경필,한재숙,小机信行,여정수,이승언,南出隆久 동아시아식생활학회 2003 동아시아식생활학회지 Vol.13 No.3

The purpose of this study was to investigate the nutritional composition of native and hybrid pork in Korea. Three different parts (ham, loin and belly) of both native and hybrid pork were used The results were as follows : The moisture content from Korean native pork was about 60.28%, while that from the loin of hybrid one was 69% and decreased in the order of ham, loin, and belly. The highest protein content of 19.71% was found in Korean native pork loin, and Korean native pork ham had a significant amount of protein of 17.80% and hybrid one had 13.14% (p< .05). The highest crude lipid, 34.44%, was found in hybrid pork belly, Korean native pork ham had a significant amount of 5.43% and hybrid pork had 2.33% (p<.05). The highest carbohydrate content of 13.28% was found in the Korean native pork belly. The amount of ash was in the order of loin, ham and belly in Korean native pork. Among the minerals, K was found the most in Korean native pork ham (654.82㎎) and hybrid one (747. 35㎎) (p<.05). Fe was higher in the Korean native pork ham (23.03㎎), loin (15.86㎎) and belly (10.80㎎) compared to the hybrid pork ham (19.04㎎), loin (11.63㎎) and belly (7.61㎎). That was significant ham, loin(p< .01) and belly(p< .05). The main free amino acids of the native and the hybrid pork in Korea were alanine, aspartic acid and lysine. While the cholesterol content was found to be high in the order of ham, belly, and loin in the Korean native pork, in the order of belly, fresh ham and loin in the hybrid pork. The cholesterol contents in ham were significantly different between the Korean native pork(789.32㎍) and the hybrid pork (538.84㎍) (p< .01).

李箱 詩의 言語美學的 考察

朴銀景 명지어문학회 1975 明知語文學 Vol.7 No.-

마이크로니들의 형태 및 적용방법이 약물의 피부 투과에 미치는 영향

임지호,이경록,이은주,조정원 충남대학교 약학대학 의약품개발연구소 2012 藥學論文集 Vol.27 No.-

The purpose of this study is to develop the microneedle using biodegradable and biocompatible polymer and to pioneer the new route of drug delivery through the research to apply the most desirable administration form of small molecules, peptides, proteins, genes, biological products including vaccines. Various biocompatible microneedles were manufactured. To apply drug to microneedle, drug formulation using ethylene vinyl acetate patch or polyacryl acid gel was developed. Drug permeation into rat skin based on the application method of patch or gel with microneedle was studied using Franz diffusion cell system. In vitro drug permeation results showed that the permeation by the application of hollow microneedle was not increased compared with solid microneedle. When G60 or G60*2 microneedle was used, the permeated amount of calcein was increased compared to when solid microneedle was used. The permeated amount of lidocaine HCl in 12 hr was increased when microneedle was used was increased. Skin irritation followed by microneedle showed there was little difference in the decrease of redness after microneedle application based on application time.