Transcriptional activation of hypoxia-inducible factor-1α by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1

Seo, Hee-Won,Kim, Eun-Jin,Na, Hyelin,Lee, Mi-Ock Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>The interplay between hypoxia-inducible factor-1α (HIF-1α) and histone deacetylase (HDACs) have been well studied; however, the mechanism of cross-talk is unclear. Here, we investigated the roles of HDAC4 and HDAC5 in the regulation of HIF-1α function and its associated mechanisms. HDAC4 and HDAC5 enhanced transactivation by HIF-1α without stabilizing HIF-1α. HDAC4 and HDAC5 physically associated with HIF-1α through the inhibitory domain (ID) that is the binding site for factor inhibiting HIF-1 (FIH-1). In the presence of these HDACs, binding of HIF-1α to FIH-1 decreased, whereas binding to p300 increased. These results indicate that HDAC4 and HDAC5 increase the transactivation function of HIF-1α by promoting dissociation of HIF-1α from FIH-1 and association with p300.</P><P><B>Structured summary:</B></P><P>MINT-6802187:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>FIH1</I> (uniprotkb:Q9NWT6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802058:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>pull down</I> (MI:0096)</P><P>MINT-6802021:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802036:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802102:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>pull down</I> (MI:0096)</P><P>MINT-6802121, MINT-6802156:</P><P><I>P300</I> (uniprotkb:Q09472) <I>physically interacts</I> (MI:0218) with <I>HIF1 alpha</I> (uniprotkb:Q16665) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P>

An S-locus receptor-like kinase in plasma membrane interacts with calmodulin in <i>Arabidopsis</i>

Kim, Ho Soo,Jung, Mi Soon,Lee, Kyunghee,Kim, Kyung Eun,Yoo, Jae Hyuk,Kim, Min Chul,Kim, Doh Hoon,Cho, Moo Je,Chung, Woo Sik Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>Calmodulin-regulated protein phosphorylation plays a pivotal role in amplifying and diversifying the action of calcium ion. In this study, we identified a calmodulin-binding receptor-like protein kinase (CBRLK1) that was classified into an S-locus RLK family. The plasma membrane localization was determined by the localization of CBRLK1 tagged with a green fluorescence protein. Calmodulin bound specifically to a Ca<SUP>2+</SUP>-dependent calmodulin binding domain in the C-terminus of CBRLK1. The bacterially expressed CBRLK1 kinase domain could autophosphorylate and phosphorylates general kinase substrates, such as myelin basic proteins. The autophosphorylation sites of CBRLK1 were identified by mass spectrometric analysis of phosphopeptides.</P><P><B>Structured summary</B></P><P>MINT-6800947:<I>CBRLK1</I> (uniprotkb:Q9ZT06) and <I>AtCaM2</I> (uniprotkb:P25069) <I>bind</I> (MI:0407) by <I>electrophoretic mobility shift assay</I> (MI:0413)</P><P>MINT-6800966:<I>AtCaM2</I> (uniprotkb:P25069) and <I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>bind</I> (MI:0407) by <I>competition binding</I> (MI:0405)</P><P>MINT-6800930:<I>CBRLK1</I> (uniprotkb:Q9ZT06) <I>binds</I> (MI:0407) to <I>AtCaM2</I> (uniprotkb:P25069) by <I>far Western blotting</I> (MI:0047)</P><P>MINT-6800978:<I>AtCaM2</I> (uniprotkb:P25069) <I>physically interacts</I> (MI:0218) with <I>CBRLK1</I> (uniprotkb:Q9ZT06) by <I>cytoplasmic complementation assay</I> (MI:0228)</P>

Silaethene CL₂Si=CHCH₂?Bu의 엔반응 : Unsaturated Compounds of 14Group Elements(Ⅸ)

김정균,박은미,구미영,박경래,손병영 東亞大學校 大學院 1992 大學院論文集 Vol.17 No.-

Silaethene Cl₂Si=CHCH₂?Bu 1, generated as a reaction intermediate by the thermal elimination of LiX from Cl₃SiCHLiCH₂?Bu 2, combines with ene-component containing compounds(propene, 2-methylpropene, DMB) to give 40∼90% yield ene-products. They can be isolated and spectroscopic identified.

A study on health and hygiene needs, strategies, and well‐being of an Aeta indigenous community in the Philippines through photovoice methods

Yang Eun‐Mi,Kim Jwakyum,Biag Al,Mangulabnan Jefferson,Dela Cruz Arlan,Sarmiento Philip Joseph 한국사회복지학회 2020 Asian Social Work and Policy Review Vol.14 No.3

On a global scale, poor access to health and social welfare services for indigenous populations has threatened their overall well-being, especially those living in remote or peripheral areas. Academics from two higher education institutions in South Korea and the Philippines conducted a community-based research in an indigenous Aeta community in the highlands of Pampanga Province in the Philippines’ Central Luzon region. Using a photovoice method and community survey, they investigated family health and hygiene as well as the current strategies this indigenous community employs to address their concerns. Results revealed that the community lacks the basic human needs and services such as water, food, shelter, education, and health service. They demonstrated, however, resilience in managing their lives by making use of the available natural resources and by drawing strength from community spirit. This study provided insights on how higher education institutions and other concerned agencies craft initiatives intended to benefit vulnerable populations and underserved communities such as the indigenous peoples.

Humanistic Approach in EFL Classes

Seo, Eun-mi 대한언어학회 2000 언어학 Vol.8 No.3

Seo Eun-mi. 2000. Humanistic Approach in EFL Classes. Journal of the Linguistic Association of Korea, 8(3), 109-122. Humanistic education comes from humanistic psychology which emphasizes the inner nature of person. Humanistic approach is learner-centered since learners' feelings and emotions are considered important. In the humanistic classroom, students like to work in pairs and groups to share their experiences and memories in a very comfortable atmosphere. In this paper, I researched the effects of humanistic education and how teachers can use various activities to motivate shy Korean students. It is highly recommended to use this method in the EFL classroom. (Howon University)

Gender, Communication and Culture

Seo, Eun Mi 대한언어학회 1997 언어학 Vol.5 No.1

Seo, Eun-Mi. 1997. Gender, Communication and Culture. Linguistics, 5-1, 45-56. Gender and communication are very interesting topic in sociolinguistics. There have existed communication problems between males and females because they have different communication styles. They also have different gender culture. It is important to accept differences between males and females in communication to avoid communication problems. In this paper, the researcher tried to make clear about the culture, gender and communication. Recommendations for successful communication are suggested. (Chonbuk Sanup University)

防風類의 比較檢討

김미경,김미정,김은영,류건영,이은미,장원희,황선희 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

Interlanguage Development in English Interrogatives by Two Korean Children

Yang, Eun Mi 대한언어학회 2000 언어학 Vol.8 No.1

Yang, Eun-Mi. 2000. Interlanguage Development in English Interrogatives by. Two Korean Children. Linguistics 8-1, 379-401. The purpose of this research is to compare the development of English question formation by the two Korean children who were attending elementary school and acquiring English in America for about 1 year and whose age difference is two years. (One is 7 years and the other is 9 years old then.) They were acquiring English mainly at school in natural environment and used Korean after school at home. Through this study I examined some prevailing hypothesis which argues for that older children are faster than younger children in acquiring second language and studied the order of acquisition of English structures according to age differences. This longitudinal study showed that both of them used 'overgeneralization' strategy for processing their interlanguage and produced systematic and rule-governed question forms, which support the hypothesis that universal cognitive mechanisms of strategies are the basis for the child's organization of the L2 system. (Paichai University)

Apartments in Korea and Japan focused on analysis of unit plan changes form 1960's to 1990's

Moon, Eun-Mi,Park, Hae-Sun 德成女子大學校 2005 德成女大論文集 Vol.34 No.-

한국과 일본은 서구에서 도입된 주거양식인 아파트가 점차 대중적으로 보급, 확산되는 과정에서 자국의 전통생활양식을 수용하여, 현재는 양국 모두 대표적인 주거유형으로 발전하게 되었다. 본 연구는 한국과 일본의 아파트 주호평면의 특징과 시대변천을 비교 · 분석하여, 주거 공간과 양식의 시대적 변화구조를 밝히는 것을 목표로 한다. 연구내용 및 결과는 다음과 같다. 첫째, 한국과 일본의 아파트 주호평면을 1960년대에서 1990년대까지 아파트의 도입과 전개, 정착과정을 중심으로 분석하였다. 둘째, 사회계층별 아파트 주호평면의 변화분석을 통해서 상류계층에서 받아들여진 평면유형이 매스컴과 대중화, 대량생산 등을 통하여 점차 중류와 하류계층에도 보급되었다는 구조를 밝힐 수 있었다. 셋째, 아파트의 정착과정에서 서구에서 도입된 아파트의 주거유형과 자국내 전통생활양식간의 갈등이 주호평면의 공간적 변용을 일으켰다. 이와같은 공간적 변용은 양국의 아파트가 대중적인 주거양식으로 받아들여지게 된 원인으로 분석되었다. 특히 이러한 경향은 전통적인 주거문화의 적용이 더 적극적이었던 한국에서 더욱 뚜렷한 것으로 나타났다.

Repellent efficacy of essential oils and plant extracts against Tribolium castaneum and Plodia interpunctella

Lee Hwa‐Eun,Hong Su Jung,Hasan Najmul,Baek Eun Joo,Kim Jun Tae,Kim Young‐Duk,Park Mi‐Kyung 한국곤충학회 2020 Entomological Research Vol.50 No.9

This study was conducted to investigate the repellent efficacy of essential oils (Origanum vulgare, Pimpinella anisum, and Tanacetum cinerariifolium) and four plant extracts (Agastache rugosa, Capsicum annuum, Citrus reticulata, and Ginkgo biloba) against Tribolium castaneum (adults and larvae) and Plodia interpunctella (larvae). Gas chromatography/mass spectrometry analysis revealed the presence of carvacrol, anethole, and jasmolin I as the predominant constituent in O. vulgare, P. anisum, and T. cinerariifolium, respectively. Furthermore, ethyl hexopyranoside, 9,12‐octadecadienoic acid, cyclopentanol, and 2‐cresol were identified in A. rugosa, C. annuum, C. reticulata, and G. biloba, respectively. The repellent efficacy of each essential oil, plant extract, and the combination of oils was evaluated using a specially designed cylinder trap for 120 h. Among the three oils, O. vulgare and T. cinerariifolium had greatest repellent efficacy against P. interpunctella larvae. T. cinerariifolium exhibited effective repellence against the adults and larvae of T. castaneum. Therefore, O. vulgare (O) and T. cinerariifolium (T) were selected for further investigation of combined effects. Two essential oils were mixed in three different ratios of OT1 (1:3), OT2 (1:1), and OT3 (3:1). The repellent efficacies of OT1 and OT2 against the adults of T. castaneum were significantly greater than that of OT3. OT1 was effective against the larvae of T. castaneum, whereas OT2 was effective against the larvae of P. interpunctella. OT1 enhanced the repellent efficacy by approximately five times against larvae of T. castaneum, compared with that of T. cinerariifolium. Overall, OT1 was selected as the best repellent substance against all the tested insects.