Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

개선한 일회성 난수를 이용한 RFID 상호인증 프로토콜

윤은준(Eun-Jun Yoon),유기영(Kee-Young Yoo) 한국정보과학회 2009 정보과학회논문지 : 정보통신 Vol.36 No.2

2008년에 Kim-Jun은 의도하지 않은 정보의 누출로 인한 악의적인 공격들 및 범죄 악용 문제점들을 해결하기 위해 일회성 난수를 이용한 RFID 상호인증 프로토콜을 제안하였다. 보안성 분석을 통하여 Kim-Jun은 제안한 프로토콜이 재전송 공격을 포함한 다양한 공격들에 안전함을 증명하였다. 하지만 본 논문에서는 그들의 주장과는 달리 그들이 제안한 프로토콜이 여전히 재전송 공격에 취약함을 증명하며, 더 나아가 동일한 연산 효율성을 보장하며 재전송 공격을 막을 수 있는 간단히 개선된 일회성 난수 기반의 RFID 상호인증 프로토콜을 제안한다. In 2008, Kim-Jun proposed a RFID mutual authentication protocol using one-time random number that can withstand malicious attacks by the leakage of important information and resolve the criminal abuse problems. Through the security analysis, they claimed that the proposed protocol can withstand various security attacks including the replay attack. However, this paper demonstrates that Kim-Jun's RFID authentication protocol still insecure to the replay attack. In addition, this paper also proposes a simply improved RFID mutual authentication protocol using one-time random number which not only provides same computational efficiency, but also withstands the replay attack.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang,Chul-Hak,Jun,Gyo,Hahm,Eun-Ryeong,Lee,Sangkyou,Park,Seyeon,Lee,Dug-Keun The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 uncleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

BMB Reports : Binding model for eriodictyol to Jun-N terminal kinase and its anti-inflammatory signaling pathway

Eun Jung Lee,Ki Woong Jeong,Areum Shin,Bong Hwan Jin,Hum Nath Jnawali,Bong Hyun Jun,Jee Young Lee,Yong Seok Heo,Yang Mee Kim 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.12

‘쓰이는 텍스트’로서의 『별건곤』과 대중문학 독자의 형성

전은경(Jun, Eun-kyung) 한국어문학회 2014 語文學 Vol.0 No.125

This paper aims to investigate the Magazine Byulgungon as “a Scriptible Text” and the formation of readers for Popular literatures. Especially readers in the Magazine Byulgungon were connected to new popular literature in 1930’s. The Magazine Byulgungon was published in Oct. 1926, and has been discontinued as Vol. 73. in Jun. 1934. At the first place, it was very first magazine for “tastes” in colonized Chosun. Tastes were related with readers’s who were the people of the middle classes or the lower classes. Actually editors in the Magazine Byulgungon would like to educate readers of the middle or low classes, reading the practical contents in the magazine. However, readers’ desires were different from intellectual editors and authors. They had desired to change the Magazine Byulgungon owing to regard it as “a Scritible Text” by Roland Gerard Barthes. According to The Reader, the Text, the Poem written by Louis M. Rosenblatt, editors’ desires and readers’ had caused “the transaction” with each other in the Magazine Byulgungon. Therefore the Magazine Byulgungon is very important text as “a Scriptible Text” that readers and editors continuously created it together. This transaction has led to several popular narratives and modern popular readers. At the same time, the Magazine Byulgungon had made popular readers prepare be able to be reders for modern literatures.

『대한민보』의 소설 정책과 근대 독서 그룹의 형성

전은경(Jun, Eun Kyung) 한국현대문학회 2014 한국현대문학연구 Vol.0 No.44

This thesis studied on relationships between the policy of newspaper and several readers’ groups. Daehaminbo(大韓民報) was Korean Daily Newspaper that published vol. 357 from Jun. 2 in 1909 to Aug. 8 in 1910. It had published by Daehanhyuphoe(大韓協會). Daehaminbo had the dual strategy for readers had read the newspaper, as considering the category of the column on literature and the arts. One was for high-level readers, so called intellectual readers who could understand Chinese letter(Hanmun, 漢文). As they had learned Chinese letter in Seodang, korean traditional education institution, they was accustomed to Chinese letter. Daehaminbo made the readers" column, Punglim(諷林) for intellectual readers. The other was for low-level readers, so called female readers who could understand Korean letter(Hangul). They traditionally had been enjoying Korean novels. It related with the serial novels in the newspaper. Serial novels had been steadily published in a front-page in Daehaminbo. As they was written by Korean letter(Hangul), female readers and the people of the lower classes who could not read and write Chinese letter(Hanmun, 漢文) were able to enjoy them. Especially Daehaminbo(大韓民報) for readers had elaborated various strategies. One of them was a serial novel column. At the first place, it was so much emphasis placed on a serial novel in the newspaper Daehaminbo(大韓民報). They tried to publish a short novel in the newspaper. The title was hua-su(花愁) that means anxiety of a flower. The Kim"s wife had a husband who kept a mistress, and she was deserted by her husband, Mr. Kim. This short novel was for female readers as a type of a domestic novel. Editors of Daehaminbo(大韓民報), therefore, considered a serial novel column for female readers who could read Hangul(Korean letters). By the way, next serial novel was changed for male readers. The serial novel unquestionably had written by Hangul(Korean letters), but contents of the serial novel were replaced by them for male readers. Daehaminbo(大韓民報) had changed male-like novels and female-like novels by turns. It had made intellectual readers read Korean novels and given birth to new reading group for intellectuals.

근대계몽기 서북지역 잡지의 편집 기획과 유학생 잡지의 상관관계

전은경(Jun, Eun-kyung) 국어국문학회 2018 국어국문학 Vol.0 No.183

근대계몽기 지식인들은 서양의 문학 개념과 일본이 받아들인 문학 개념을 그대로 모방하고 답습한 것이 아니라 이 문학 개념에 대해 변형하면서 수용했다. 이 논문은 그러한 개념 정립의 과정을 국내 잡지와 유학생 잡지의 영향 관계를 중심으로 파악해 보고자 했다. 서북도인들을 중심으로 국내 학회지를 간행한 『서우』와『서북학회월보』가 유학생 잡지 『태극학보』와 『대한흥학보』 등에 어떠한 영향을 받았고, 또 어떻게 변화하고 있는지 살펴보았다. 국내 학회지는 유학생 잡지와의 교류를 통해 새로운 문학적 장치와 편집 체계를 받아들였다. 또한 이 가운데 그대로 모방만을 하는 것이 아니라 스스로 그 개념을정립하고자 하는 노력을 보여주었다. <문예>면에는 한문체 산문이나 논설류 등을 포함시키고, <담총>, <가담>에는 새로운 문학적 가능성이 보이는 글을 배치했다. 이러한 구분은 국내 지식인들 스스로 이전 글과 대화체 서사 등의 글이 “다르다”라고 인식하고 있었기 때문에 가능했다. 또한 이러한 “다름”에 대한 인식은 이들이 유학생들과 교류하며, 유학생 잡지에 직접 참여했기 때문에 가능한 일이었다. 근대문학은 한순간 갑자기 성립된 것이 아니라, 이러한 새로움에 대한 발견과 “다름”에 대해 인식할 수 있는 시각을 정립해 가는 과정에서 진행되었다. This paper is to analyze a birth of modern concept about modern literatures in modern enlightenment era. The goal of this theory is to study the establishing process about the concept of modern literature between magazines for students studying in Japan and those for intellectuals in Joseon. The magazine Seowoo(『西友』) and Seobukhakhuiwolbo(『西北學會月報』) had influenced on the birth of modern novels and had made various narratives in the modern enlightenment era. These magazines were published by intellectuals who had lived in north-west area in Joseon. The magazine Seobukhakhuiwolbo had two editors; one was Park Eun-sik who was a Confucian scholar, the other was Kim Won-keok who had studied in japan. The editor, Kim Won-keok had established new titles of the magazine Seobukhakhuiwolbo as he had been influenced by the magazine Taegukhakbo(『太極學報』) that had published by students studying in Japan. Korean students studying in Japan had tried to formulate the concept of the modern literature, and also domestic intellectuals had tried to established the concept of new literatures. Therefore, domestic intellectuals had made the column of <liberal arts> and <conversation collection> as the title of magazine. They had included dialogue narratives that had implied in various contemporary lives.

Repellent efficacy of essential oils and plant extracts against Tribolium castaneum and Plodia interpunctella

Lee Hwa‐Eun,Hong Su Jung,Hasan Najmul,Baek Eun Joo,Kim Jun Tae,Kim Young‐Duk,Park Mi‐Kyung 한국곤충학회 2020 Entomological Research Vol.50 No.9

This study was conducted to investigate the repellent efficacy of essential oils (Origanum vulgare, Pimpinella anisum, and Tanacetum cinerariifolium) and four plant extracts (Agastache rugosa, Capsicum annuum, Citrus reticulata, and Ginkgo biloba) against Tribolium castaneum (adults and larvae) and Plodia interpunctella (larvae). Gas chromatography/mass spectrometry analysis revealed the presence of carvacrol, anethole, and jasmolin I as the predominant constituent in O. vulgare, P. anisum, and T. cinerariifolium, respectively. Furthermore, ethyl hexopyranoside, 9,12‐octadecadienoic acid, cyclopentanol, and 2‐cresol were identified in A. rugosa, C. annuum, C. reticulata, and G. biloba, respectively. The repellent efficacy of each essential oil, plant extract, and the combination of oils was evaluated using a specially designed cylinder trap for 120 h. Among the three oils, O. vulgare and T. cinerariifolium had greatest repellent efficacy against P. interpunctella larvae. T. cinerariifolium exhibited effective repellence against the adults and larvae of T. castaneum. Therefore, O. vulgare (O) and T. cinerariifolium (T) were selected for further investigation of combined effects. Two essential oils were mixed in three different ratios of OT1 (1:3), OT2 (1:1), and OT3 (3:1). The repellent efficacies of OT1 and OT2 against the adults of T. castaneum were significantly greater than that of OT3. OT1 was effective against the larvae of T. castaneum, whereas OT2 was effective against the larvae of P. interpunctella. OT1 enhanced the repellent efficacy by approximately five times against larvae of T. castaneum, compared with that of T. cinerariifolium. Overall, OT1 was selected as the best repellent substance against all the tested insects.

Methazolamide 복용 후 발생한 Stevens-Johnson Syndromex

정종호,이은정,김태형,전원선,우광진,양미연,강병일,은준영,추은주,전민혁 순천향의학연구소 2010 Journal of Soonchunhyang Medical Science Vol.16 No.1

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