子官癌細胞(HeLa)에 대한 血府逐瘀湯과 數種 抗癌劑의 倂用投與 效果

殷載淳,蘇俊魯 又石大學校 1992 論文集 Vol.14 No.-

The studies were conducted to investigate the combined effects of Hyulbu Chuko Tang (HCT) and several anti-cancer drugs. The effects of HCT and several anti-cancer drugs on the proliferation of HeLa Colls, human cervical cell line, was estimated by MTT colorimetric assays. The 50% inhibitory concentrations of mitomycin C(MMC), cisplatin(CPT), mercaptopurine (MCP) and 5-fluorouracil(5-FU) on HeLa cell were 0.313, 0.565, 22.745 and 28.325/㎖, repectively. HCT extract inhibited the proliferation of HeLa cell at 10^-6-10^-3g/㎖. The inhibitory action of the MCP treated group on the proliferation of HeLa cell was increased by HCT and MCP. When the mice were treated by MMC, the number of leukocyte was decreased significantly at the 1st and 3rd day, but recovered at the 7th day. In the groups of MMC treated with HCT, the number of leukocytes was increased significantly than the group of MMC treated only at the 1st and 3rd day. The HCT extract increased the number of plaque forming cells(PFC), but the MMC treated group decreased the number of PFC. The combined treatment of MMC and HCT increased the number of PFC significantly than the MMC treated group. The HCT extract increased the proliferation of T cells, but the MMC treated group decreased the proliferation of T cells. The combined treatment of MMC and HCT increased the T cell proliferation significantly than MMC treated group.

Piperic Acid Amide 誘導體의 微生物學的 硏究 : Studies on the Synthesis and Antibacterial Activity of Piperic Acid Analogues

殷載淳 又石大學校 1983 論文集 Vol.5 No.-

5-(3, 4-methylenedioxypheny)-2, 4-pentadienoic acid[piperic acid], 3, 4-methylenedioxy cinnamic acid, 3, 4-methylenedioxy benzoic acid, 5-phenyl 2, 4-pentadienoic acid, cinnamic acid, benzoic acid and various amides and esters of these acids were synthesized. The fifty-six compounds synthesized were tested for their antibacterial activity was shown as follows;the number of synthesized compounds, ⅩⅩⅩⅤ, ⅩⅩⅩⅨ, ⅩL, ⅩLⅠ, ⅩLⅢ, ⅩLⅥ, ⅩLⅨ and LⅢ were active against Staphylococcus aureus, ⅩⅩⅩⅨ, ⅩL, ⅩLⅥ and ⅩLⅨ were active against Bacillus subtilis, ⅩⅠ, ⅩⅩⅡ, ⅩⅩⅢ, ⅩⅩⅥ, ⅩⅩⅦ, ⅩⅩⅩⅧ, ⅩⅩⅩⅨ, ⅩLⅥ, LⅩⅧ, ⅩLⅨ, LⅡ, LⅣ and LⅥ were native against Escherichia coli, and ⅩⅩⅩⅥ, ⅩⅩⅩⅦ, ⅩLⅥ, ⅩLⅦ, ⅩLⅧ, LⅣ, LⅤ and LⅥ were active against Pseudomonas aeruginosa.

括數根 水溶性 蛋白質의 細胞毒性

殷載淳,鄭甲基,梁在憲,權鎭,吳贊鎬,全焄 又石大學校 1995 論文集 Vol.17 No.-

The purpose of this research was to investigate effect of water-soluble protein of Trichosanthes kirillowii(TKP) on the proliferation of human tumor cells. TKP inhibited the proliferation of HeLa cells and KHOS-NP cells. The inhibitory activity of doxorubicin on HeLa cells and its of mitomycin C on KHOS-NP cells were increased by the combination of TKP. TKP did not affect on the proliferation of Balb/c 3T3 cells, but inhibited the proliferation of mouse spleen cells and human lymphocyte at 1 ㎍/ml. These results suggest that TKP bas the cytotoxicity on HeLa and KHOS-NP cells, and increase the cytotoxicity of doxorubicin or mitomycin C.

3T3-L1 세포주에서 분비하는 인체 암세포 성장억제 단백질에 대한 연구

은재순,권진 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Ingibition of the growth of human cancer cells by proteins secreted from 3T3-L1 cells was investigated in the present study. The growth of human cancer cells was inhibited by co-culture with 3T3-L1 cells under 10% FBS and DME, GIT and serumless medium, respectively. The conditioned medium of cultured 3T3-L1 cells under serumless medium was concentrated 100-fold through an ultrafiltration cell with a 10,000 molecular weight cutoff at 4℃ under positive pressure using nitrogen (3T3-L1 EM). 3T3-L1 EM inhabited the growth of HrRa, Hep G2, KHOS-Np, A431 andMCF-7 cells. 3T3-L1 EM was purified with FPLC, DEAE-ion exchange chromatography and pheny-sepharose chromatography. The major protein of 3T3-L1 EM has a molecular weight of 66,000-69,000 in SDS-PAGE analysis. The results suggest that the inhibitory activity of 3T3-L1 EM appears to be due to some protein(m.w. 66,000-68,000) secreted by 3T3-L1 cells.

일할 수밖에 없는 여성노인의 다중차별 경험에 관한 연구

정순둘,장명선,이은진,송보가,김재민 이화여자대학교 젠더법학연구소 2021 이화젠더법학 Vol.13 No.2

본 연구는 노동시장에서 일할 수밖에 없는 여성노인의 다중차별 경험을 파악하여 이들의 노동권을 보호하고 차별을 해소하기 위한 방안을 마련하는데 목적이 있다. 이를 위해 노인집중 고용 업종에 종사하는 60세 이상 여성노인 14명을 대상으로 심층면접조사를 진행하였다. 본 연구 결과, 일할 수밖에 없는 여성노인들은 노동시장의 입직 단계에서부터 성별, 연령, 건강의 다중차별에 의한 상시적인 고용불안과 사회안전망에서의 배제를 경험하였다. 또한 일하는 조직의 불평등 지배체제 특성으로 일상화된 고용불안, 열악한 노동조건과 복지제도의 미비, 노인친화적이지 않은 노동 강도와 근무 제공, 노인 노동 평가 절하에 따른 조직 위계 문화와 관행, 과도한 노동 제공의 요구와 성희롱의 일상화, 조직 내에서 이해를 대변하는 데 어려움을 경험하는 것으로 나타났다. 본 연구 결과를 바탕으로 일할 수밖에 없는 여성노인의 다중차별을 해소하기 위한 법과 제도의 개선, 사회안전망 강화, 여성노인 노동자 이해대변단체 조직화 지원을 제언하였다. This study aims to (i) identify the experience of multiple discrimination among older women, who have no choice but to work in the labor market; (ii) protect their labor rights; and (iii) resolve discrimination. To conduct this study, we performed an in-depth interview survey with 14 older women aged 60 or older who are engaged in the elderly intensive employment sector. Under the result of this study, older women, who have no choice but to work, have experienced constant employment insecurity and exclusion from the social safety net due to multiple discrimination in gender, age, and health from the employment stage in the labor market. In addition, because of inequality organizations, they have experienced as follows: employment insecurity that became common, poor labor conditions and inadequate welfare systems, unfriendly labor intensity and provision of work, organizational hierarchical culture and practices due to devaluation of elderly labor, demand for excessive labor provision and sexual harassment routinely, and difficulties in representing understanding within the organization. Based on the results of this study, therefore, this study provides suggestions to resolve the multiple discrimination of older women, who have no choice but to work, including but no limited to, improve laws and systems, strengthen the social safety net, and organize support for the organization of interest groups for older women workers.

3, 4-Methylenedioxy Cinnamic Acid Amide 誘導體의 合成 및 藥理作用에 관한 硏究

殷載淳 又石大學校 1984 論文集 Vol.6 No.-

In order to search more active and less toxic compound in comparison with the central nervous depressant activity of piperine, 3,4-methylenedioxy cinnamic acid amides were synthesized by condensation of 3,4-methylenedioxy cinnamoyl chloride with alkyl or aryl amines, and N-acyl-3,4-methylenedioxy cinnamimides were synthesized by condesation of 3,4-methylenedioxy unsubstiuted amide with acyl chlorides. The bioassay results revealed that, among the compounds synthesized, 3,4-methylenedioxy cinnamoyl monoethylamide exhibited more potent protective activity against pentylenetetrazoleinduced convulsion, 3,4-methylenedioxy cinnamoyl unsubstituted amide, monoethylamide and anilide exhibited more potent protective activity against strychnine-induced convulsion, and all of the compunds except 3,4-methylenedioxycinnamoyl unsubstituted amide and monoethylamide showed less acute toxicity than that of piperine.

托裡消毒飮이 傷處治癒에 미치는 影響

殷載淳,黃甲洙 又石大學校 1992 論文集 Vol.14 No.-

These experiments were conducted to investigate the wound healing action of Taklee-Sodok-Eum(TSE) water ext. TSE extract did not affect on the paper disk granuloma formation and mouse paw edema induced by histamine, but inhibited on the leakage of evans blue into peritoneal cavity. Using proliferation of Balb/c 3t3 fibroblast cell line as an in vitro model of granulation tissue formation, the ability of TSE to stimulate cellualar proliferation of fibroblast cells was investigated. When the cells were seeded at 1 × 10 exp (4) cells/well, Balb/c 3T3 cells were reached to the late exponential phase at 3rd day. Under the conditions established above, TSE increased the proliferation of Balb/c 3T3 cells at concentration of 10^-6 g/ml. The treatment of 10^-6 g/ml of TSE did not influence on the DNA synthesis, but decreased the protein synthesis of the cells. The 10% serum from TSE treated mice(500㎎/㎏/day for 4 days) increased the proliferation of Balb/c 3T3 fibroblast markedly, and did not influence on the DNA synthesis and protein sythesis of the cells. The results suggest that wound healing in vivo will be agumented by the treatment of TSE. In addition, TSE may be of practical therapeutic use in wound healing.

능이버섯의 藥理作用에 관한 硏究

殷載淳,이중원,黃甲洙 又石大學校 1990 論文集 Vol.12 No.-

The pharmacological effects of Sarcodon aspratus extract were investigated. The extract promoted contraction of isolated stomach and ileum, and this contraction was blocked by atropine(7×10^-7M) and cyproheptadine (1.5×10^-7M). The maximum effective dose of the extract was 1.5×10^-3g/㎖. The extract promoted the peristaltic movement of small intestine, and this movement was blocked by atropine. The extract accelerated the secretion of histamine in serum and gastric acid. The serum glucose and total cholesterol levels in alloxan-induced mice were significantly decreased by oral administration of the extract for 7 days. The pharmacological effects of the extract on the central nervous system were little.

자궁암세포(HeLa)에 대한 사군자탕과 수종 항암제의 병용투여 효과

은재순,김현욱,소준노,오찬호,이송재 又石大學校 1993 論文集 Vol.15 No.-

The studies were conducted to investigate the combined effects of Sa Kunja Tang(SKT) and several anti-cancer drugs. The effects of SKT and several anti-cancer drugs on the proliferation of HeLa cells, human cervical cell line, was estimated by MTT colorimetric assays. The SKT extract inhibited the proliferation of HeLa cell at 10^-4g/ml. The inhibitory action of the mitomycin C(MMC), mercaptopurine(MCP) and 5-fluorouracil(5-Fu) treated group, respectively, on the proliferation of HeLa cell was increased by the SKT. When the mice were treated by the MMC, the number of leukocyte was decreased significantly at the 1st and 3rd day, but recovered at the 7th day. In the groups of the MMC treated with the SKT, the number of leukocytes was increased significantly than the group of the MMC treated only at the 3rd day. The SKT extract decreased the thymus weight of mice. The SKT extract increased the number of plaque forming cells(PFC), but the MMC treated group decreased the number of PFC. The combined treatment of MMC and the SKT increased the number of PFC significantly than the MMC treated group. The SKT extract increased the proliferation of T cells, but the MMC treated group decreased the proliferation of T cells. The combined treatment of MMC and the SKT increased T cell proliferation significantly than the MMC treated group. In conclusion, the results presented in this paper suggest that the SKT extract can decrease the dose of anti-cancer drugs on HeLa cell, and recover the side dffects of the MMC, such as leukopenia and immunosuppresion, without any intercalating the anti-proliferative action of the MMC in vivo.

靑皮가 마우스의 免疫細胞에 미치는 影響

殷載淳,吳贊鎬,鄭鉉雨 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

靑皮는 疏肝破氣 및 消積化滯하는 작용이 있는 약물로 대부분 氣滯로 인한 증상들에 사용되고 있지만 임상상 活血之劑와 配合하여 氣滯血瘀나 血瘀脇痛등에 應用되고 있다. 氣滯는 각종의 스트레스등으로 인하여 발생되어 血行을 不暢하게 하고, 심지어는 正氣까지도 손상시킨다. 正氣의 손상은 바로 免疫機能의 低下라고도 말할 수 있는데, 免疫이란 非自己로 인식된 異常細胞를 처리함으로써 個體의 恒常性을 維持하려는 현상을 말한다. 면역에 先天的 免疫과 T림프구 및 B림프구가 관여하는 特異性 免疫이 있고, 免疫作用에 관여하는 細胞로는 特異性 免疫에 관여하는 免疫細胞外에도 Macrophage, NK cell, 수지상 세포 및 랑게르한스 세포등을 들 수 있다. 뇌의 기능은 심장에서 방출되는 혈액의 공급에 의해 이루어지는데, 만약 혈액의 공급이 제대로 이루어지지 않으면 많은 질환들이 발생한다. 그 중 虛血性 腦疾患은 충분한 양의 혈액이 뇌를 공급되지 않거나 공급되고 있는 혈액내의 산소농도가 떨어져 발생하는 것으로써 임상적으로는 運動麻痺·知覺痲痺등 神經機能 消失등의 증상을 보이기 때문에 東醫學的으로는 이를 風病이라 하고, 風病은 대체적으로 陰虛나 血虛등으로도 발생할 수 있어 陰虛動風證 또는 血虛生風證등의 病證들이 있다. 그리하여 각종 氣滯로 인한 氣滯則血滯가 발생되거나 또는 正氣가 손상되어 氣虛則血虛하기 때문에 陰虛나 血虛등으로 인한 運動麻痺나 知覺麻痺가 발생한다. 이러한 이유등으로 本 硏究者들은 혈류장애 질환치료에 사용되는 韓藥材中 靑皮의 작용을 규명하는 硏究의 일환으로 靑皮가 면역기능에 어떠한 영향을 미치는가에 대하여 알아보기 위하여 동결건조된 靑皮를 마우스에 1일 1회씩 7일간 투여한 후 T-lymphcyte의 Apoptosis와 sub-population, 그리고 proliferation 및 Peritoneal macrophage의 nitric oxide 생성과 phagocytic activity를 관찰한 결과 DNA fragmentation과 sub-population에는 별다른 영향을 주지 않았지만 proliferation에 있어서는 증식이 촉진되었고, 복강 macrophage의 nitric oxide 생성과 phagocytic activity에 있어서는 NO는 감소하였으나 phagocytic activity는 현저히 증가하였다. 이는 靑皮가 NO 생성을 억제하여 phagocytic activity를 증가시킨 결과라 思料된다. 이상과 같은 實驗結果들을 통해서 볼 때 靑皮는 臨床的으로 각종 氣滯로 인한 正氣損傷과 血液循環障碍가 동시에 나타날 때에만 正氣를 보충해주는 役割을 할 수 있을 것으로 사료된다. The purpose of this research was to investigate effects of Citri Reticulatae Viride Pericarpium water extract(CRVP) on immunocytes in mice. The administration of CRVP did not affect apoptosis and sub-population of mouse T-lymphocytes, but increased the proliferation of T-lymphocytes. CRVP decreased nitric oxide production from mouse peritoneal macrophage, but increased a phagocytic activity of macrophages. These results suggest that CRVP does not affect T cell mediated immunity, but increases the phagocytic activity of peritoneal macrophages.