Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

광촉매 산화반응을 이용한 트리클로로에틸렌(TCE) 처리에 관한 연구

정창훈,조정구,송민종,전의찬,최금찬 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.1

Photocatalyzed degradation of trace level trichloroethylene(TCE) and toluene in air were carried out over near UV illuminated titanium dioxide(anatase) pellet in a flow reactor. This study was investigated the effects of humidity and oxygen. Inlet concentrations for TCE was injected with the ranges of 10~100 ppm. TCE photooxidatino was very rapid under our experimental conditions, and ~100% conversion was achieved for TCE concentration examined up to 70 ppm as a single air contaminant. An important finding was that competitive adsorption between humidity and oxygen has a significant effect on the oxidation rate.

액체소듐속에서 소듐-물반응에 의한 재질의 Wastage현상

정경채,정지영,김병호,김태준,최병해,최종현,김의식 한국공업화학회 2003 응용화학 Vol.7 No.1

In this study we observed and analyzed the corrosion phenomena in leak site and the effect on temperature at the target surface of heat transfer tube material by sodium-water reaction through water leakage in liquid sodium atmosphere. Surrounding of leak hole of the specimen was damaged by the wastage phenomena due to corrosion and erosion of material, and hole size by damage of leak site was extended from 150㎛ to about 200㎛. Also, the damaged size appeared in the target surface was observed to about 1000㎛ diameter. The temperature of target surface increased about 70~80℃ by the heat of sodium-water reaction even with the small leakage of 15㎖ water. From the results of EPMA and EDX analysis, damaged area are found to covered with complex compounds such as (NaOH+Na_2O) FexOy or (NaCrO_2+nA_2CrO4) mixed up with the sodium oxides by sodium-water reaction and the Fe or Cr element by matrix of tube sodium oxides by matrix of tube material.

골프수요 시차집중률의 구조적 변화 분석 : The East-Coastal Region of Gangwon Province

정의선,최성범 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

This study aims to analyze structural changes of seasonality in golf tourism demand and suggest alternatives on the development of effective demand of golf tourism for the east-coastal region of Gangwon province. The results of the analysis of absolute growth rate and relative composition rate indicated growth, and public golf courses are growing more rapidly than courses for members. National average reveals a stable structure of demand except for January and February, but in the case of the east-coastal region of Gangwon province, peak season barely lasts for May, June, July, August and October. On the basis of the above examination, the author suggests alternatives for attractive tourism destinations in the east-coastal region of Gangwon province. It is required that the central government or local government should establish a induced tourism policy for the creation of effects. strategic de-marketing is necessary, because mass marketing is accompanied with enviornmental pollution, destruction of regional values, magnification of the dependence-oriented economic structure, and so forth. In order to link tourism resources, it is important that the coastal scenery in golf courses is built and the central and local government should develop tourism, leisure and sports belt for visitors and residents.

대장암종에서 암관련 유전자들의 Promoter Methylation과 암종 발생 및 진행과의 관게에 대한 연구

정동준,이정은,민영기,조성우,백무준,양승하,김의한,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The colorectal corcinoma is one of the most common malignant tumor in Korea and its carcinogenic mechanism has not been elucidated exactly yet. So far, the most common carcinogenic mechanism has been known to be multiple genetic changes. But, the CpG island hypermethylation has been revealed to be one of the most important carcinogenic mechanism second to the genetic changes. The epigenetic changes characteristically imprint and transfer to the next generation without changes of nucleotide sequences. When the CpG island of promoter or 5'- exon 1 of a specific gene is methylated by DNA methyltrasferases (DNMTs), the gene expression is inhibited. Carcinogenic mechansisms by promoter methylation has been well known, but the progression of the cancer has not been elucidated yet. The molecular researches on the carcinogenic mechanisms largely depended on the tumor tissue containing various components of cell, due to difficulties in harvesting pure homogeneous tumor cells. Promoter methylatonof the cancer related genes as APc, hMLH2, p16, DAP-K and MGMT in 50 cases of sporadic colorectal carcinoma was studied. The pure normal colorectal epithelial cells, and cancer cells from superficial and deep invasive portion were harvested respectively by laser captured microdissection(LCM), and DNA was extracted from them. The promoter methylation was studied by methylation specific poymerase reaction (MSP) after bisulfite modification of the DNA and the expression of the genes by immunohistochemistry. The results suggests that promoter methylation of the cancer related genes are closely related with the colorectal carcinogenesis and it occurrs from early phase of carcinogenesis, progressing constantly to the late stage. And the promoter methylation of normal epithelial cells increased by age. The p15, known to be the genes related to hematologic malignancy, was closely related to the colorectal carcinoma as well.

파노라마 방사선사진에서 하악관과 이공의 관찰되는 정도

정성진,최의환,김재덕 대한구강악안면방사선학회 2001 Imaging Science in Dentistry Vol.31 No.3

Purpose : To determine the head position that the superior border of the mandibular canal as well as mental foramen can be more clearly visualized in panoramic radiography Materials and Methods : Ten dry mandibles were radiographed bilaterally using PM 2002 CC panoramic machine. A 20 mm thick aluminium filter was added to the slit collimator to obtain radiographs with acceptable density. The specimens were tilted by 2, 4, 6, 8, and 10 degrees downward with and without radiopaque markers. Radiopaque markers were inserted into the mandibular canals and the mental canals of each side of the specimens to serve as reference image when assessing the radiographs. The visibility of the mandibular canal and the mental foramen was estimated by 4 observers on all radiographs. The obtained results were analyzed statistically. Results : Mandibular canals were significantly more clearly visible in the radiographs with 4 and 6 degree downward position on both sides (P〈0.05). Mental foramens were significantly more clearly visible in the radiographs with 4, 6, and 8 degree downward on right side and 6 degree downward on left side (P〈0.05). There was not significant difference between right and left sides. Conclusion : Panoramic radiographs with 4 to 6 degree downward tilting could be valuable in locating the mandibular canal as well as the mental foramen. (Korean J Oral Maxillofac Radiol 2001; 31 : 153-8)

저장조건에 따른 Leucaena 화분의 발아력

정병룡,박의호 대구대학교 과학기술연구소 1996 科學技術硏究 Vol.3 No.1

Leucaena is the common name for Leucaena leucocephala which is a tropical leguminous tree with a potential value as a source of wood production, high quality forage, and revegetation of hillslopes. In spite of the plant's value, both the differences of pod setting ratio among Leucaena species and the differences of flowering period limited breeding of high quality species of Leucaena plant. To overcome these problems this study was conducted to find the possibility of long term storage of Leucaena pollen by using vacuum and low temperatures. Pollen viability decreased after 1.5 days after storage at room temperature. The same trend was observed for the pollen stored at refrigerator (91℃). Pollen viability, however, was maintained longer with decreasing storage temperature to that of freezer (-17℃). Vacuum treatment also helped to maintain pollen viability either at room or refrigerator temperatures. The ratio of exploded pollen showed the same pattern as the percentage of normally germinated pollen stored under different conditions except the pollen stored at refrigerator. Average tube lengths of germinated pollens were 4.3 and 3.6 mm for the pollen stored at freezer and refrigerator with vacuum, respectively. In conclusion, the viability of Leucaena pollen could be extended by storing under vacuum at freezing temperatures.

단순겹치기 접착이음의 파괴역학 파라미터해석

정의섭,이원 崇實大學校 生産技術硏究所 1996 論文集 Vol.26 No.1

The boundary element elastostatic analysis of interface crack between the two dissimilar materials, was described. The method to determine parameter of fracture mechanics(stress intensity factor, energy release rate), was studied. The parameter of fracture mehanics for single bonded lap-joint analyzed by the present method and boundary element method(BEM), can be applied to confirm fracture criterion crack.

建設에 있어서 經營情報體制 導入에 關한 基礎的 硏究

鄭義容,李重雨,楊淳甲 啓明大學校 産業技術硏究所 1988 産業技術硏究所 論文報告集 Vol.10 No.-

The circumstance of construction are more complicated and more diversified so that the managers may save their money and time in their management. In this regard, this paper will present a basic theory of Total Management information System in construction.

영상특수효과 적용 사례 연구

정의태 경운대학교 산업기술연구소 2002 産業技術硏究論文誌 Vol.4 No.2

It is not long time ago that we tried to put something into our graphic design or motion graphics. In late 1960s the 'Work' which is called special effects demanded huge amount of labor. Producers had to print every single frame and arrange to animate. Those kinds of works were real time consuming jobs. Furthermore, results were not even quite accurate. Since those kinds of reason, the effects could not catch the viewers interest effectively in terms of motion graphics design. As time goes on, both technology and sense of screen design have been changed dramatically. People in general realize that this is another type of possibility for industry.