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Cloning of a novel endogenous promoter for foreign gene expression in Phaeodactylum tricornutum
ERDENE OCHIR ERDENEDOLGOR,신복규,HUDA MD NAZMUL,김다혜,이은하,송대근,김윤미,김상민,판철호 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.6
Phaeodactylum tricornutum is a model diatom, and its genomic sequence data and expressed sequence tag databases are available. This study was to discover a new endogenous promoter that drives strong constitutive expression of a protein of interest in P. tricornutum. To find promoter candidates, the intracellular proteins of P. tricornutum grown to stationary phase were extracted and identified by LC–MS/MS. Glutamine synthetase (GLNA) was one of the most abundantly expressed proteins during the stationary phase. Promoter is usually located on 5′ upstream region of open reading frame of the gene. Thus, two fragments of 5′ upstream region of the GLNA gene as putative promoters, 501 and 997 bp long, were amplified and cloned into enhanced Green Fluorescent Protein (eGFP) reporter systems. The constructed reporter systems were transformed into P. tricornutum and the eGFP expression levels were compared to those of reporter systems using the promoters of fcpA (fucoxanthin chlorophyll a/c binding protein A) and CIP1 (putative replication-associated proteins of a Chaetoceros lorenzianus-infecting DNA virus) as controls. The expression of eGFP driven by either GLNA promoter (501 and 997 bp) was linearly related to cell density, and eGFP was expressed constitutively regardless of the cultivation phase. The eGFP expression level driven by the GLNA promoters was at least 4 times higher than the fcpA-driven eGFP expression level at the stationary phase. The 501 and 997 bp regions of the GLNA promoter had similar activity patterns for transcribing the downstream gene. These results indicate that at least 501-bp region of the GLNA promoter can be used as a strong constitutive promoter in genetic engineering of P. tricornutum.
Erdene-Ochir Ganbold,Jin-Ho Park,Kwang-Su Ock,주상우 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.2
We studied the detection of the Hg(II) concentration in an aqueous solution using rhodamine dyes on citrate-reduced Au nanoparticles (NPs). The quenching effect from Au NPs was found to decrease as the Hg(II) concentration increased under our experimental conditions. As the fluorescence signals intensified, the surface-enhanced Raman scattering (SERS) intensities reduced on the contrary due to less rhodamine dyes on Au NPs as the Hg(II) concentration increased. The rhodamine 6G (Rh6G) and rhodamine 123 (Rh123) dyes were examined via fluorescence and SERS measurements depending on Hg(II) concentrations. Fast and easy fluorescence detection of an Hg (II) concentration as low as a few ppm could be achieved by naked eye using citrate-reduced Au NPs.
Raman Spectroscopy of Biphenyl-4,4'-dithiol and p-Terphenyl-4,4″-dithiol on Gold Surfaces#
Erdene Ochir Ganbold,주상우 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.3
We compared the adsorption behaviors of 1,4-benzenedithiol (BDT), biphenyl-4,4′-dithiol (BPDT), and p-terphenyl-4,4″-dithiol (TPDT) on gold (Au) surfaces. To check the surface orientations and possibility of multilayer formation, surface-enhanced Raman scattering was employed for the three aromatic dithiols on gold nanoparticles (AuNPs). The Raman spectra of BDT and BPDT on AuNPs were examined, whereas those of TPDT could be obtained on an Au-coated nanostructured silicon plate. Unlike BDT, BPDT and TPDT did not exhibit a ν(S–H) band at 2560 cm−1. Because of the additional benzene rings, multilayer formation is improbable for BPDT and TPDT under colloidal conditions.
Khos-Erdene Khishigbayar 한양대학교 세라믹연구소 2014 Journal of Ceramic Processing Research Vol.15 No.6
The activated carbon’s enormous capacity to absorb and retain significant quantities of many organic compounds has led to its use as a purifying medium. An anion is adsorbed onto the activated carbon, and the metal ion interacts with the anion, effectively being absorbed by an absorbate. Observation of the metal ion adsorption, displayed that complex organic agents and pre-absorbed anions can affect the amount of adsorption. In this study, we have tried to improve the purity of alumina precursor using activated carbon for 1.5 hr by stirring at 200 rpm at 82 o C.