DEPTOR Expression Negatively Correlates with mTORC1 Activity and Tumor Progression in Colorectal Cancer

Lai, Er-Yong,Chen, Zhen-Guo,Zhou, Xuan,Fan, Xiao-Rong,Wang, Hua,Lai, Ping-Lin,Su, Yong-Chun,Zhang, Bai-Yu,Bai, Xiao-Chun,Li, Yun-Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

The mammalian target of rapamycin (mTOR) signaling pathway is upregulated in the pathogenesis of many cancers, including colorectal cancer (CRC). DEPTOR is an mTOR inhibitor whose expression is negatively regulated by mTOR. However, the role of DEPTOR in the development of CRC is not known. The aim of this study was to investigate the expression of DEPTOR and mTORC1 activity (P-S6) in a subset of CRC patients and determine their relation to tumor differentiation, invasion, nodal metastasis and disease-free survival. Here, Immunohistochemical expression of P-S6 (S235/236) and DEPTOR were evaluated in 1.5 mm tumor cores from 90 CRC patients and in 90 samples of adjacent normal mucosa by tissue microarray. The expression of P-S6 (S235/236) was upregulated in CRC, with the positive rate of P-S6 (S235/236) in CRC (63.3%) significantly higher than that in control tissues (36.7%, 30%) (p<0.05). P-S6 (S235/236) also correlated with high tumor histologic grade (p=0.002), and positive nodal metastasis (p=0.002). In contrast, the expression level of DEPTOR was correlated with low tumor histological grade (p=0.006), and negative nodal metastasis (p=0.001). Interestingly, P-S6 (S235/236) expression showed a significant negative association with the expression of DEPTOR in CRC (p=0.011, R= -0.279). However, upregulation of P-S6 (S235/236) (p=0.693) and downregulation of DEPTOR (p=0.331) in CRC were not significantly associated with overall survival. Thus, we conclude that expression of DEPTOR negatively correlates with mTORC1 activity and tumor progression in CRC. DEPTOR is a potential marker for prognostic evaluation and a target for the treatment of CRC.

Analytical Behavior of Eccentrically Loaded Concrete Encased Steel Columns Subjected to Standard Fire including Cooling Phase

Er-Feng Du,Gan-Ping Shu,Xiao-Yong Mao 한국강구조학회 2013 International Journal of Steel Structures Vol.13 No.1

A nonlinear 3-D finite element analysis (FEA) model was developed to predict the behavior of eccentrically loaded concrete encased steel (CES) columns subjected to ISO-834 standard fire including heating and cooling phases. The finite element model has been validated against published tests conducted at elevated temperatures. Comparisons between the predicted results and the test results show that this model can accurately predict the behavior of CES columns under fire. The FEA model was then used to investigate the typical temperature-time curve and mid-height lateral deformation-time curve of eccentric compression CES columns in a complete loading history including initial loading, heating and cooling. It is shown that the temperature delay is obvious at the inner layers of concrete. The fire resistance of a CES column should be checked for the full process of fire exposure until temperatures everywhere in the column start to decrease. The lateral deformation of the column still gradually increases during the cooling phase and the column may fail during that phase. There is a large residual deformation after the fire exposure. Furthermore, the variables that influence the behavior of the CES columns under fire were investigated in parametric studies. It is found that the main parameters which influence the lateral deformation-time curve of the column during the full process of fire exposure are load ratio, slenderness ratio, duration time, depth to width ratio and steel ratio, and the main parameters which influence the residual deformation ratio of the column after fire are load ratio, duration time, cross-sectional depth and steel ratio.

병원 임상간호사의 직무특성 및 직무만족과 이직의도와의 관계

어금숙,허혜경,김기경,조용자,김은희,김순자 병원간호사회 2004 임상간호연구 Vol.9 No.2

Purpose: The purpose of this study was to investigate the relationship between job characteristics, job satisfactions, and intents to leave among hospital nurses. Method: The participants for this study were 248 nurses. The survey instruments included turnover intentions scale used Lawler (1983), Job Diagnostic Survey developed by Hackman & Oldman (1980) and Index of Work Satisfaction developed by Stamps and Piedmonte (1978). Data were analyzed using of means, percentages, t-test, ANOVA, Pearson correlation coefficient with SPSS program and using Baron and Kennys approach (1986). Result: The mean for job characteristics scores were 53.13, the mean job satisfaction scores 121.42, the turnover intentions scores 14.75. Statistically significant differences were found between job satisfaction, turnover intentions and age, work experience, job position. There were significant positive correlations between job characteristics and job satisfaction. There were not significant correlations between job characteristics and turnover intentions. There were significant negative correlations between job satisfaction and turnover intentions. The mediating role of job satisfaction in the relationship between job satisfaction and turnover intentions was not supported. Conclusion: The major predictor of intention to leave was job satisfaction, but not job characteristics.

Study of the Correlation of Plasma Resonance and the Refractive Index to Dielectric Dispersion in the Complex Plane

Xiao-Yong Zhou,Yan Shen,Er-Tao Hu,Jian-Bo Chen,Yuan Zhao,Ming-Yu Sheng,Jing Li,Yu-Xiang Zheng,Hai-Bin Zhao,Liang-Yao Chen,Wei Li,Xun-Ya Jiang,이영백,David W. Lynch 한국광학회 2013 Current Optics and Photonics Vol.17 No.1

Based on the dispersive feature of the dielectric function of noble metals and the wave vector conservation in physics, both the plasma effect and the complex refractive index, which are profoundly correlated to the complex dielectric function and permeability, have been studied and analyzed. The condition to induce a bulk or a surface plasma in the visible region will not be satisfied, and there will be one solution for the real and the imaginary parts of the refractive index, restricting it only to region I of the complex plane. The results given in this work will aid in understanding the properties of light transmission at the metal/dielectric interface as characterized by the law of refraction in nature.

Serum miR-21 Expression in Human Esophageal Squamous Cell Carcinomas

Cai, Er-Hui,Gao, Yong-Xin,Wei, Zhong-Zhi,Chen, Wei-Ying,Yu, Ping,Li, Ke Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

To investigate the relationship between serum miRNA-21 (miR-21) expression in esophageal squamous cell carcinomas (ESCCs) and its clinicopathologic features, a 1:1 matched case-control study including 21 patients with ESCC and 21 age- and gender-matched healthy controls was carried out. Serum specimens were taken from all subjects. Total RNA was extracted and the stem-loop real time polymerase chain reaction was used to measure serum miR-21 in both groups. Clinical parameters were assessed to determine associations with serum miR-21 concentrations. Serum miR-21 expression in ESCC samples was significantly higher than in paired cancer-free samples (P<0.05). Metastasis was associated with mir-21 expression in serum (P<0.05), ESCC patients with metastasis having 8.4-fold higher serum miR-21 concentrations than healthy controls. There were no statistically significant associations between miR-21 expression and clinicopathologic parameters, such as gender (P>0.05), age (P>0.05), tumor location (P>0.05), cell differentiation (P>0.05), TNM staging (P>0.05), whether chemo/radiotherapy had been administered (P>0.05), or whether surgery had been performed (P>0.05). These findings suggest that the detection of microRNA-21 in serum might serve as a new tumor biomarker in diagnosis and assessment of prognosis of ESCCs.

Age-associated changes in pancreatic exocrine secretion of the isolated perfused rat pancreas

Zheng-er Jiang,ChengZhe Jiang,Baihui Chen,Chin Su Koh,Jun-Hwan Yong,Dae-Hun Park,Moo-Ho Won,Yun-Lyul Lee 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.1

Gut functions, such as gastrointestinal motility, gastric secretion and pancreatic secretion, were reduced with age. Glucose tolerance is impaired, and the release of insulin and β-cell"s sensitivity on glucose are reduced with age. However, a lot of controversial data have been reported as insulin concentrations after glucose ingestion are either higher or no different in elderly and young subjects. Thus, this study was aimed to investigate whether aging could affect pancreatic exocrine secretion and its action mechanisms. An isolated perfused rat pancreatic model was used to exclude the effects of external nerves or hormones. Pancreatic secretion was increased by CCK under 5.6 mM glucose background in the isolated perfused pancreas of young (3 months), 12 months and 18 months aged rats. There was no significant difference between young and aged rats. In 3 months old rats, CCK-stimulated pancreatic secretion was potentiated under 18 mM glucose background. However, the potentiation effects of endogenous insulin and CCK were not observed in 12 and 18 months old rats. Exogenous insulin also potentiated CCKstimulated pancreatic secretion in 3 months old rats. Similarly, exogenous insulin failed to potentiate CCK-stimulated pancreatic secretion as that of 3 months old rats. Wet weight of pancreas and amylase content in pancreatic tissue were not changed with age. These results indicate that pancreatic exocrine secretion is reduced with age and endogenous insulin secretion and/or action is involved in this phenomenon.

Sensitivity Properties of PID Controllers

Meng Joo Er,Soy Yong Chua 대한전자공학회 1994 JTC-CSCC : Joint Technical Conference on Circuits Vol.- No.-

A Novel Multirate Output Linear Quadratic Gaussian Controller for Linear Time-Invariant Systems

Meng Joo Er,Say Yong Chua 대한전자공학회 1994 JTC-CSCC : Joint Technical Conference on Circuits Vol.- No.-

Roles of Non-cholinergic Intrapancreatic Nerves, Serotonergic Nerves, on Pancreatic Exocrine Secretion in the Isolated Perfused Rat Pancreas

Jiang, Zheng Er,Shin, Bich-Na,Kim, In-Hye,Lee, Hyun-Joo,Yong, Jun-Hwan,Lee, Min-Jae,Won, Moo-Ho,Lee, Yun-Lyul The Korean Society of Pharmacology 2011 The Korean Journal of Physiology & Pharmacology Vol.15 No.5

It has been rereported that axons which display 5-hydroxytryptamine (5-HT) immunoreactivity are abundant in the pancreas and the majority of serotonergic axons terminate within intrapancreatic ganglia, islet and acini. This histological result strongly suggests that intrapancreatic serotonergic nerves could affect to the pancreatic endocrine and exocrine secretion. Thus, this study was aimed to investigate whether intrapancreatic serotonergic nerves could affect pancreatic exocrine secretion and an action mechanism of the intrapancreatic serotonergic nerves. The rats were anesthetized with a single injection of urethane. The median line and the abdominal aorta was carefully dissected and cannulated with PE-50 tubing just above the celiac artery, and then tightly ligated just below the superior mesenteric artery. The pancreatic duct was also cannulated with Tygon microbore tubing. With the addition of serotonin, pancreatic volume flow and amylase output were significantly inhibited electrical field stimulation (EFS). On the other hand, pancreatic volume flow and amylase output were significantly elevated in EFS with the addition of spiperone. EFS application, however, pancreatic volume flow and amylase output had no significant change in cholecystokinin (CCK) alone when serotonin was applied under a 5.6 mM glucose background. Pancreatic volume flow and amylase output under 18 mM glucose background were significantly elevated in CCK plus serotonin than in CCK alone. These data suggest that intrapancreatic serotonergic nerves play an inhibitory role in pancreatic exocrine secretion and an important role in the insulin action or release.

In situ observation of atomic movement in a ferroelectric film under an external electric field and stress

Lee, Hyeon Jun,Guo, Er-Jia,Min, Taewon,Hwang, Seung Hyun,Lee, Su Yong,Dorr, Kathrin,Lee, Jaekwang,Jo, Ji Young Tsinghua Press, co-published with Springer-Verlag 2018 NANO RESEARCH Vol.11 No.7

<P>Atomic movement under application of external stimuli (i.e., electric field or mechanical stress) in oxide materials has not been observed due to a lack of experimental methods but has been well known to determine the electric polarization. Here, we investigated atomic movement arising from the ferroelectric response of BiFeO3 thin films under the effect of an electric field and stress in real time using a combination of switching spectroscopy, time-resolved X-ray microdiffraction, and in situ stress engineering. Under an electric field applied to a BiFeO3 film, the hysteresis loop of the reflected X-ray intensity was found to result from the opposing directions of displaced atoms between the up and down polarization states. An additional shift of atoms arising from the linearly increased dielectric component of the polarization in BiFeO3 was confirmed through gradual reduction of the diffracted X-ray intensity. The electric-field-induced displacement of oxygen atoms was found to be larger than that of Fe atom for both ferroelectric switching and increase of the polarization. The effect of external stress on the BiFeO3 thin film, which was controlled by applying an electric field to the highly piezoelectric substrate, showed smaller atomic shifts than for the case of applying an electric field to the film, despite the similar tetragonality.</P>