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( Fathiah Zakham ),( Imane Chaoui ),( Driss Messaoudi ),( Mustapha Ennaji ),( Mohammed Abid ),( Mohammed El Mzibri ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Tuberculosis (TB) remains one of the most prevalent infectious diseases worldwide especially in developing countries, where the highest burden of TB is found. This is further exemplifi ed by the high mortality and morbidity due to this disease. In this context, the emergence of multi drug resistant TB and extremely drug resistant TB cases had worsened the situation. Indeed, the rapid diagnosis of MDR TB is a critical issue for TB management. This study is an attempt to establish a rapid diagnosis of MDR TB by sequencing the target fragments of rpoB gene which linked to resistance against Rifampicin and KatG gene and inhA promoter region, which are associated to resistance to Isoniazid. Methods: For this purpose, 133 sputum samples of TB patients from Morocco, where enrolled in this study. 100 samples were collected from new cases, the rest 33 were previously treated patients (drug relapse or failure, chronic) and did not respond to anti TB drugs after a suffi cient duration of treatment. All samples were subject to rpoB katG and pinhA mutations analysis by PCR and DNA sequencing. Results: Molecular analysis showed that 7 strains were isoniazid monoresistant and 17 were rifampicin monoresistant. MDR TB strains were identifi ed in 9 cases (6.8%). Among them, 8 were traditionally diagnosed as critical cases: four chronic and four drug-relapse cases. The last strain was isolated from a new case. Conclusions: The implementation of molecular approaches for direct diagnosis of MDR-TB, as a part of the routine analysis in the laboratories of health care institutions, will be of great benefi t to adapt treatment regimens, limit the dissemination of MDR-TB strains and limit the emergence of XDR-MTB strain for better management of TB in Morocco.
( Malak Jamil ),( Hasnae Debbarh ),( Amal Kabit ),( Moha Ennaji Lic ),( Loic Koumba ),( Ismail Kaarouch ),( Moha Zarqaoui ),( Wassim Rhazi Senhaji ),( El Mehdi Hissane ),( Brahim Saadani ),( Pierre Va 대한산부인과학회 2023 Obstetrics & Gynecology Science Vol.66 No.3
Objective To compare the degree of efficiency between density gradient centrifugation (DGC) method and an extended horizontal swim-up (SU) method. Methods A total of 97 couples undergoing in vitro fertilization were enrolled in the study. Semen samples were divided into three aliquots and treated using DGC, extended horizontal SU, and combined methods. DNA fragmentation and chromatin decondensation were detected in native semen samples and their three corresponding aliquots. The corresponding mature oocytes of each semen sample were divided into two sibling cultures. The first sibling culture was microinjected with semen pellets from DGC, and the second sibling culture was microinjected with semen pellets from the combination of both methods. Fertilization rate and embryonic development were assessed at day 3. Results DNA fragmentation and chromatin decondensation was significantly low in DGC and extended horizontal SU samples; however, the rates of DNA fragmentation and chromatin decondensation were significantly lower in extended horizontal SU samples than in DGC samples. The lowest rates of DNA fragmentation and chromatin decondensation corresponded to the samples treated with both methods. The highest rates of DNA fragmentation and chromatin decondensation corresponded to the samples treated with DGC. No significant difference was found in the fertilization rate or day 3 embryos between sibling cultures. Conclusion The combination of DGC and the extended horizontal SU techniques is best for giving the lowest rates of sperm DNA fragmentation and chromatin decondensation.