Cytotoxicity of Trichoderma spp. Cultural Filtrate Against Human Cervical and Breast Cancer Cell Lines

El-Rahman, Atef Abd El-Mohsen Abd,El-Shafei, Sally Mohamed Abd El-Aziz,Ivanova, Elena Vladimirovna,Fattakhova, Alfia Nurlimanovna,Pankova, Anna Victorovna,El-Shafei, Mohamed Abd El-Aziz,El-Morsi, El-M Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Trichoderma spp. are known as a rich source of secondary metabolites with biological activity belonging to a variety of classes of chemical compounds. These fungi also are well known for their ability to produce a wide range of antibiotic substances and to parasitize other fungi. In search for new substances, which might act as anticancer agents, the overall objective of this study was to investigate the cytotoxic effects of Trichoderma harzianum and Trichoderma asperellum cultural filtrates against human cervical and breast cancer cell lines (HeLa and MCF-7 cells respectively). To achieve this objective, cells were exposed to 20, 40, 60, 80 and 100 mg/ml of both T. harzianum cultural filtrate (ThCF) and T. asperellum cultural filtrate (TaCF) for 24h, then the cell viability and the cytotoxic responses were assessed by using trypan blue and 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assays. Morphological changes in cells were investigated by phase contrast inverted microscopy. The results showed that ThCF and TaCF significantly reduce the cell viability, have cytotoxic effects and alter the cellular morphology of HeLa and MCF-7 cells in a concentration dependent manner. A concentration of 80 and 100mg/ml of ThCF resulted in a sharp decline in the cell viability percent of HeLa and MCF-7 respectively (25.2%, 26.5%) which was recorded by trypan blue assay. The half-maximal inhibitory concentrations ($IC_{50}$) of ThCF and TaCF in HeLa and MCF-7 were recorded as 16.6, 12.0, 19.6 and 0.70mg/ml respectively by MTT assay. These results revealed that ThCF and TaCF have a substantial ability to reduce the viability and proliferation of human cervical and breast cancer cells.

Environmental analysis of present and future fuels in 2D simple model marine gas tubines

El Gohary, M. Morsy The Society of Naval Architects of Korea 2013 International Journal of Naval Architecture and Oc Vol.5 No.4

Increased worldwide concerns about fossil fuel costs and effects on the environment lead many governments and scientific societies to consider the hydrogen as the fuel of the future. Many researches have been made to assess the suitability of using the hydrogen gas as fuel for internal combustion engines and gas turbines; this suitability was assessed from several viewpoints including the combustion characteristics, the fuel production and storage and also the thermodynamic cycle changes with the application of hydrogen instead of ordinary fossil fuels. This paper introduces the basic environmental differences happening when changing the fuel of a marine gas turbine from marine diesel fuel to gaseous hydrogen for the same power output. Environmentally, the hydrogen is the best when the $CO_2$ emissions are considered, zero carbon dioxide emissions can be theoretically attained. But when the $NO_x$ emissions are considered, the hydrogen is not the best based on the unit heat input. The hydrogen produces 270% more $NO_x$ than the diesel case without any control measures. This is primarily due to the increased air flow rate bringing more nitrogen into the combustion chamber and the increased combustion temperature (10% more than the diesel case). Efficient and of course expensive $NO_x$ control measures are a must to control these emissions levels.

Utilization of alternative marine fuels for gas turbine power plant onboard ships

El Gohary, M. Morsy,Seddiek, Ibrahim Sadek The Society of Naval Architects of Korea 2013 International Journal of Naval Architecture and Oc Vol.5 No.1

Marine transportation industry is undergoing a number of problems. Some of these problems are associated with conventional marine fuel-oils. Many researchers have showed that fuel-oil is considered as the main component that causes both environmental and economic problems, especially with the continuous rising of fuel cost. This paper investigates the capability of using natural gas and hydrogen as alternative fuel instead of diesel oil for marine gas turbine, the effect of the alternative fuel on gas turbine thermodynamic performance and the employed mathematical model. The results showed that since the natural gas is categorized as hydrocarbon fuel, the thermodynamic performance of the gas turbine cycle using the natural gas was found to be close to the diesel case performance. The gas turbine thermal efficiency was found to be 1% less in the case of hydrogen compared to the original case of diesel.

Effect of simulated double cycle welding on HAZ microstructure for HSLA steels

El-Kashif, Emad F.,Morsy, Morsy A. Techno-Press 2018 Advances in materials research Vol.7 No.3

High Strength low alloy steels containing various levels of C, Nb and Mn were used and for each of which, a simulated double thermal cycle was applied with the same first peak temperature and different second peak temperatures to produce HAZ microstructure corresponding to multi-pass weld. Effect of double cycle second temperature on the microstructure was observed and compared with single cycle results obtained from previous works, it was found that the percentage of martensite austenite constituent (MA) increases by Nb addition for all steels with the same Mn content and the increase in Mn content at the same Nb content shows an increase in MA area fraction as well. MA area fraction obtained for the double cycle is larger than that obtained for the single cycle for all steels used which imply that MA will have great role in the brittle fracture initiation for double cycle and the inter-pass temperature should be controlled for medium and high-carbon Mn steel to avoid large area fraction of MA. The beneficial effects of Niobium obtained in single pass weld were not observed for the double cycle or multi pass welds.

Heavy Metals Biosorption from Aqueous Solution by Endophytic Drechslera hawaiiensis of Morus alba L. Derived from Heavy Metals Habitats

( Mervat Morsy Abbas Ahmed El-gendy ),( Naziha M. Hassanein ),( Hussein Abd El-hay Ibrahim ),( Doaa H. Abd El-baky ) 한국균학회 2017 Mycobiology Vol.45 No.2

The ability of dead cells of endophytic Drechslera hawaiiensis of Morus alba L. grown in heavy metals habitats for bioremoval of cadmium (Cd²⁺), copper (Cu²⁺), and lead (Pb²⁺) in aqueous solution was evaluated under different conditions. Whereas the highest extent of Cd²⁺ and Cu²⁺ removal and uptake occurred at pH 8 as well as Pb²⁺ occurred at neutral pH (6-7) after equilibrium time 10 min. Initial concentration 30 mg/L of Cd⁺² for 10 min contact time and 50 to 90 mg/L of Pb²⁺ and Cu²⁺ supported the highest biosorption after optimal contact time of 30 min achieved with biomass dose equal to 5 mg of dried died biomass of D. hawaiiensis. The maximum removal of Cd²⁺, Cu²⁺, and Pb² equal to 100%, 100%, and 99.6% with uptake capacity estimated to be 0.28, 2.33, and 9.63 mg/g from real industrial wastewater, respectively were achieved within 3 hr contact time at pH 7.0, 7.0, and 6.0, respectively by using the dead biomass of D. hawaiiensis compared to 94.7%, 98%, and 99.26% removal with uptake equal to 0.264, 2.3, and 9.58 mg/g of Cd²⁺, Cu²⁺, and Pb²⁺, respectively with the living cells of the strain under the same conditions. The biosorbent was analyzed by Fourier Transformer Infrared Spectroscopy (FT-IR) analysis to identify the various functional groups contributing in the sorption process. From FT-IR spectra analysis, hydroxyl and amides were the major functional groups contributed in biosorption process. It was concluded that endophytic D. hawaiiensis biomass can be used potentially as biosorbent for removing Cd²⁺, Cu²⁺, and Pb²⁺ in aqueous solutions.

Utilization of alternative marine fuels for gas turbine power plant onboard ships

M. Morsy El Gohary,Ibrahim Sadek Seddiek 대한조선학회 2013 International Journal of Naval Architecture and Oc Vol.5 No.1

Marine transportation industry is undergoing a number of problems. Some of these problems are associated with conventional marine fuel-oils. Many researchers have showed that fuel-oil is considered as the main component that causes both environmental and economic problems, especially with the continuous rising of fuel cost. This paper investigates the capability of using natural gas and hydrogen as alternative fuel instead of diesel oil for marine gas turbine, the effect of the alternative fuel on gas turbine thermodynamic performance and the employed mathematical model. The results showed that since the natural gas is categorized as hydrocarbon fuel, the thermodynamic performance of the gas turbine cycle using the natural gas was found to be close to the diesel case performance. The gas turbine thermal efficiency was found to be 1% less in the case of hydrogen compared to the original case of diesel.

Genome Shuffling of Mangrove Endophytic Aspergillus luchuensis MERV10 for Improving the Cholesterol-Lowering Agent Lovastatin under Solid State Fermentation

( Mervat Morsy Abbas Ahmed El-gendy ),( Hind A. A. Al-zahrani ),( Ahmed Mohamed Ahmed El-bondkly ) 한국균학회 2016 Mycobiology Vol.44 No.3

In the screening of marine mangrove derived fungi for lovastatin productivity, endophytic Aspergillus luchuensis MERV10 exhibited the highest lovastatin productivity (9.5 mg/gds) in solid state fermentation (SSF) using rice bran. Aspergillus luchuensis MERV10 was used as the parental strain in which to induce genetic variabilities after application of different mixtures as well as doses of mutagens followed by three successive rounds of genome shuffling. Four potent mutants, UN6, UN28, NE11, and NE23, with lovastatin productivity equal to 2.0-, 2.11-, 1.95-, and 2.11-fold higher than the parental strain, respectively, were applied for three rounds of genome shuffling as the initial mutants. Four hereditarily stable recombinants (F3/3, F3/7, F3/9, and F3/13) were obtained with lovastatin productivity equal to 50.8, 57.0, 49.7, and 51.0 mg/gds, respectively. Recombinant strain F3/7 yielded 57.0 mg/gds of lovastatin, which is 6-fold and 2.85-fold higher, respectively, than the initial parental strain and the highest mutants UN28 and NE23. It was therefore selected for the optimization of lovastatin production through improvement of SSF parameters. Lovastatin productivity was increased 32-fold through strain improvement methods, including mutations and three successive rounds of genome shuffling followed by optimizing SSF factors.

Environmental analysis of present and future fuels in 2D simple model marine gas tubines

M. Morsy El Gohary 대한조선학회 2013 International Journal of Naval Architecture and Oc Vol.5 No.4

Increased worldwide concerns about fossil fuel costs and effects on the environment lead many governments and scientific societies to consider the hydrogen as the fuel of the future. Many researches have been made to assess the suitability of using the hydrogen gas as fuel for internal combustion engines and gas turbines; this suitability was assessed from several viewpoints including the combustion characteristics, the fuel production and storage and also the thermodynamic cycle changes with the application of hydrogen instead of ordinary fossil fuels. This paper introduces the basic environmental differences happening when changing the fuel of a marine gas turbine from marine diesel fuel to gaseous hydrogen for the same power output. Environmentally, the hydrogen is the best when the CO2emissions are considered, zero carbon dioxide emissions can be theoretically attained. But when the NOx emissions are considered, the hydrogen is not the best based on the unit heat input. The hydrogen produces 270% more NOx than the diesel case without any control measures. This is primarily due to the increased air flow rate bringing more nitrogen into the combustion chamber and the increased combustion temperature (10% more than the diesel case). Efficient and of course expensive NOx control measures are a must to control these emissions levels.

A Review of the Potential Replacements of HCFC,HFCs Using Environment-Friendly Refrigerants

A. R. El-Sayed,M. El Morsi,N. A. Mahmoud 대한설비공학회 2018 International Journal Of Air-Conditioning and Refr Vol.26 No.3

The adequate and efficient performance of HVAC systems are signs of luxury and human comfort, and the improvement of their performance has been the target of continuous researches. Choosing the suitable refrigerant is the main parameter in matching the system components, selecting the type of heat exchangers, the compressor, the expansion device and the suitable lubricant. The theoretically ideal refrigerant is the one having zero ozone depletion potential (ODP), low global warming potential (GWP), nontoxic, nonflammable, has appropriate thermodynamic and heat transfer properties and is compatible with any type of lubricating oil. Chlorinated, fluorinated refrigerants, zeotropic and azeotropic mixtures satisfy many requirements, but have high ODP and GWP and are not compatible with all types of oil. Hydrocarbons (HCs) satisfy all the requirements except being highly flammable. This work reviews previous research aiming to find substitutes for the environmentally harmful refrigerants by other environmentally friendly ones and compare their performance in various HVAC appliances.

Experimental Investigation of a Walk-in Refrigerator Performance Using R290 as a Retrofit for R22

A. R. El-Sayed,M. El Morsi,N. A. Mahmoud 대한설비공학회 2018 International Journal Of Air-Conditioning and Refr Vol.26 No.4

An experimental performance study is performed on a refrigeration system equipped with a scroll compressor and tested with R22 using an electronic expansion valve (EEV) as an expansion device and controlled by proportional-integral-derivative (PID) control method. The system is tested at −12∘C, −8∘C, −4∘C, 0∘C and 4∘C evaporator air temperature and 20∘C, 25∘C and 30∘C condenser inlet water temperature at 50 Hz compressor driving frequency and 50Hz evaporator fans driving frequency. R22 reached the set point at all temperatures except −12∘C evaporator temperature with 30∘C condenser temperature due to the severe increase in the compressor discharge temperature that could result in the lubricating oil burnout. Then, the system is retrofitted with a reciprocating compressor especially designed to be used with R290 and tested with R290 at the same evaporator and condenser temperatures using EEV as an expansion device. R290 reached all set points at all evaporator and condenser temperatures. Also, R290 was able to reach all evaporator temperatures at higher condenser temperatures, 35∘C and 40∘C, and this will be discussed in future work. The results show that using R290, the pulldown time decreases by 30.3–71.4%, the ON time ratio decreases by 1–23.6%, the compressor discharge temperature decreases by 46.6–81∘C, the refrigerant mass flow rate decreases by 28.8–50.4%, VRC decreases by 15.2–32.5%, the compressor power consumption decreases by 34.4–44.3% and coefficient of performance (COP) increases by 35–115.5%.