Cytotoxicity of Trichoderma spp. Cultural Filtrate Against Human Cervical and Breast Cancer Cell Lines

El-Rahman, Atef Abd El-Mohsen Abd,El-Shafei, Sally Mohamed Abd El-Aziz,Ivanova, Elena Vladimirovna,Fattakhova, Alfia Nurlimanovna,Pankova, Anna Victorovna,El-Shafei, Mohamed Abd El-Aziz,El-Morsi, El-M Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Trichoderma spp. are known as a rich source of secondary metabolites with biological activity belonging to a variety of classes of chemical compounds. These fungi also are well known for their ability to produce a wide range of antibiotic substances and to parasitize other fungi. In search for new substances, which might act as anticancer agents, the overall objective of this study was to investigate the cytotoxic effects of Trichoderma harzianum and Trichoderma asperellum cultural filtrates against human cervical and breast cancer cell lines (HeLa and MCF-7 cells respectively). To achieve this objective, cells were exposed to 20, 40, 60, 80 and 100 mg/ml of both T. harzianum cultural filtrate (ThCF) and T. asperellum cultural filtrate (TaCF) for 24h, then the cell viability and the cytotoxic responses were assessed by using trypan blue and 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assays. Morphological changes in cells were investigated by phase contrast inverted microscopy. The results showed that ThCF and TaCF significantly reduce the cell viability, have cytotoxic effects and alter the cellular morphology of HeLa and MCF-7 cells in a concentration dependent manner. A concentration of 80 and 100mg/ml of ThCF resulted in a sharp decline in the cell viability percent of HeLa and MCF-7 respectively (25.2%, 26.5%) which was recorded by trypan blue assay. The half-maximal inhibitory concentrations ($IC_{50}$) of ThCF and TaCF in HeLa and MCF-7 were recorded as 16.6, 12.0, 19.6 and 0.70mg/ml respectively by MTT assay. These results revealed that ThCF and TaCF have a substantial ability to reduce the viability and proliferation of human cervical and breast cancer cells.

Validation of a New Design of Tellurium Dioxide-Irradiated Target

Aziz Fllaoui,Younes Ghamad,Brahim Zoubir,Zinel Abidine Ayaz,Aissam El Morabiti,Hafid Amayoud,El Mahjoub Chakir 한국원자력학회 2016 Nuclear Engineering and Technology Vol.48 No.5

Production of iodine-131 by neutron activation of tellurium in tellurium dioxide (TeO2) material requires a target that meets the safety requirements. In a radiopharmaceutical production unit, a new lid for a can was designed, which permits tight sealing of the target by using tungsten inert gas welding. The leakage rate of all prepared targets was assessed using a helium mass spectrometer. The accepted leakage rate is ≤ 10−4 mbr.L/s, according to the approved safety report related to iodine-131 production in the TRIGA Mark II research reactor (TRIGA: Training, Research, Isotopes, General Atomics). To confirm the resistance of the new design to the irradiation conditions in the TRIGA Mark II research reactor's central thimble, a study of heat effect on the sealed targets for 7 hours in an oven was conducted and the leakage rates were evaluated. The results show that the tightness of the targets is ensured up to 600°C with the appearance of deformations on lids beyond 450°C. The study of heat transfer through the target was conducted by adopting a one-dimensional approximation, under consideration of the three transfer modes—convection, conduction, and radiation. The quantities of heat generated by gamma and neutron heating were calculated by a validated computational model for the neutronic simulation of the TRIGA Mark II research reactor using the Monte Carlo N-Particle transport code. Using the heat transfer equations according to the three modes of heat transfer, the thermal study of I-131 production by irradiation of the target in the central thimble showed that the temperatures of materials do not exceed the corresponding melting points. To validate this new design, several targets have been irradiated in the central thimble according to a preplanned irradiation program, going from 4 hours of irradiation at a power level of 0.5 MW up to 35 hours (7 h/d for 5 days a week) at 1.5 MW. The results show that the irradiated targets are tight because no iodine-131 was released in the atmosphere of the reactor building and in the reactor cooling water of the primary circuit.

Activated Nitrites in Heterocyclic Synthesis: Syntheses of Thiazole, Pyrazole and 4H-l,4-Benzothiazine Derivatives

El-Taweel, Fathy Mohamed Abdel-Aziz,Hadi-Mashaly, Mohamed-Abdel,Ali-Elagamey, Abdel-Ghani The Pharmaceutical Society of Korea 1990 Archives of Pharmacal Research Vol.13 No.3

4-Arylazo-3-phenyl-5-aminopyrazoles (5a, b) and substituted hydroxythiazoles 8a, b were synthesized from the reaction of 4a, b with hydrazine hydrate and mercaptoacetic acid respectively. Compounds 5a, b and 8a, b were also obtained from coupling of 2a, b with 6 and 7, respectively. 4H-1, 4-Benzothiazine 11 was prepared from 1 and 10. The resaction of the diazonium salts 2a-c with diethyl 3-amino-2-cyanopenet-2-en-1, 5-dicarboxylate 12 was also reported.

Hepatitis C Virus Associations with Non Hodgkin's Lymphoma: Insights on Inflammation/Angiogenesis and CD Markers

El-Maadawy, Eman A,Talaat, Roba M,Sadek, Rawia F,El-Sherbini, Sherif M,Abdel-Bary, Naser,Abdel-Aziz, Amal A Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9

We aimed to investigate any association between hepatitis C virus (HCV) infection and non-Hodgkin's lymphoma (NHL) in the view of cytokines that control inflammation/angiogenesis and their correlation with certain CD markers. NHL patients with or without HCV infection were studied. CD5, CD30, CD3, CD20 and CD45 were immunohistochemically evaluated. Plasma levels of vascular endothelial and platelet derived growth factors (VEGF, and PDGF), tumor necrosis factor (TNF-${\alpha}$), transforming growth factor (TGF-${\beta}$), interleukin-6 (IL-6), IL-8, IL-4, IL-12 and interferon gamma (IFN-${\gamma}$) were detected by enzyme-linked immunosorbent assay (ELISA). HCV+ve NHL patients showed a significant reduction in VEGF, PDGF, IFN-${\gamma}$, CD5 and CD45 and a significant increase in IL-12 and IL-8. In conclusion, there was a significant change in cytokine secretion and expression of CD markers in HCV+ve NHL patients. Based on our results, HCV infection in NHL patients requires more in-depth investigations to explore any role in lymphoma progression.

A Self-Consistent Semi-Analytical Model for AlGaAs/InGaAs PMHEMTs

Abdel Aziz, M.,El-Banna, M.,El-Sayed, M. The Institute of Electronics and Information Engin 2002 Journal of semiconductor technology and science Vol.2 No.1

A semi-analytical model based on exact numerical analysis of the 2DEG channel in pseudo-morphic HEMT (PMHEMT) is presented. The exactness of the model stems from solving both Schrodinger's wave equation and Poisson's equation simultaneously and self-consistently. The analytical modeling of the device terminal characteristics in relation to the charge control model has allowed a best fit with the geometrical and structural parameters of the device. The numerically obtained data for the charge control of the channel are best fitted to analytical expressions which render the problem analytical. The obtained good agreement between experimental and modeled current/voltage characteristics and small signal parameters has confirmed the validity of the model over a wide range of biasing voltages. The model has been used to compare both the performance and characteristics of a PMHEMT with a competetive HEMT. The comparison between the two devices has been made in terms of 2DEG density, transfer characteristics, transconductance, gate capacitance and unity current gain cut-off frequency. The results show that PMHEMT outperforms the conventional HEMT in all considered parameters.

A Self-Consistent Semi-Analytical Model for AlGaAs/InGaAs PMHEMTs

M. Abdel Aziz,M. El-Banna,M. El-Sayed 대한전자공학회 2002 Journal of semiconductor technology and science Vol.2 No.1

Role of Some Phenylthiourea Derivatives as Corrosion Inhibitors for Carbon Steel in HCl Solution

Fouda, Abd El-Aziz El-Sayed,Hussein, Ahmed Korean Chemical Society 2012 대한화학회지 Vol.56 No.2

Five derivatives of phenylthiourea namely: 1-(4-methoxyphenyl)-3-phenylthiourea (1), 1-(4-methylphenyl)-3- phenylthiourea (2), 1-(4-bromophenyl)-3-phenylthiourea (3), 1-(4-chlorophenyl)-3-phenylthiourea (4) and 1-phenylthiourea (5) have been evaluated as new inhibitors for the corrosion of carbon steel in 2 M HCl solution using potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) techniques. Potentiodynamic polarization measurements showed that these derivatives are mixed-type inhibitors. The inhibition efficiency was found to increase with inhibitor concentration and decreases with rise in temperature. The thermodynamic parameters of adsorption and activation were determined and discussed. Nyquist plots showed depressed semicircles with their centre below real axis. The adsorption process of studied derivatives on carbon steel surface obeys Temkin adsorption isotherm. The synergistic effect of these derivatives and some anions is discussed from the viewpoint of adsorption models. The electrochemical results are in good agreement with the calculated quantum chemical HOMO and LUMO energies of the tested molecules.

Ginger Extract as Green Corrosion Inhibitor for Steel in Sulfide Polluted Salt Water

Fouda, Abd El-Aziz S.,Nazeer, Ahmed Abdel,Ibrahim, Mohamed,Fakih, Mohamed Korean Chemical Society 2013 대한화학회지 Vol.57 No.2

Extract of ginger has been evaluated as a green inhibitor for the corrosion of steel in sulfide polluted NaCl solution using potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and electrochemical frequency modulation (EFM) techniques. Potentiodynamic polarization measurements showed that this extract acts as a mixed type inhibitor but mainly inhibits the cathodic reaction. The inhibition efficiency was found to increase with inhibitor concentration reaching to approximately 83.9% using 250 ppm of ginger. Nyquist plots show a single capacitive loop in uninhibited and inhibited solutions. From EFM the causality factors are very close to theoretical values which indicate that the measured data are of good quality. The adsorption process of the studied extract on steel surface obeys Temkin adsorption isotherm. The results obtained from the different electrochemical techniques were in good agreement which prove the validity of these tools in measurement of corrosion rate. Ginger extract has no effect on Escherichia Coli and can be applied safely on waste water treatment plants.

Aqueous Extract of Coriander Seeds as Green Corrosion Inhibitor for 304 Stainless Steel in Hydrochloric Acid Solutions

Fouda, Abd El-Aziz. S.,Rashwan, Salah M.,Abo-Mosallam, Hytham A. Korean Chemical Society 2014 대한화학회지 Vol.58 No.1

Corrosion inhibition of 304 stainless steel (SS) in 1 M HCl by aqueous extract of coriander seeds was studied using weight loss, potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and electrochemical frequency modulation (EFM) techniques. Values of inhibition efficiency obtained are dependent upon the concentration of extract and temperature. Generally, inhibition was found to increase with inhibitor concentration, but decrease with temperature. Physical adsorption mechanism has been proposed for the inhibition with Langmuir adsorption isotherm obeyed. Values of activation energy of the inhibited corrosion reaction of 304 SS are greater than the value obtained for the blank. Thermodynamic consideration reveals that adsorption of aqueous extract of coriander seeds 304 SS surface is spontaneous.

Electrochemical Adsorption Properties and Inhibition of Zinc Corrosion by Two Chromones in Sulfuric Acid Solutions

Fouda, Abd El-Aziz S.,Nazeer, Ahmed Abdel,Saber, Ahmed Korean Chemical Society 2014 대한화학회지 Vol.58 No.2

The electrochemical behavior and corrosion inhibition of zinc in 0.5 M $H_2SO_4$ in the absence and presence of some chromones has been investigated using weight loss, potentiodynamic polarization, electrochemical impedance spectroscopy (EIS) and electrochemical frequency modulation (EFM) techniques. The presence of these investigated compounds in the corrosive solutions decrease the weight loss, the corrosion current density, and double layer capacitance but increases the charge transfer resistance. Polarization studies were carried out at room temperature, and showed that all the studied compounds act as mixed type inhibitors with a slight predominance of cathodic character. The effect of temperature on corrosion inhibition has been studied and the thermodynamic activation and adsorption parameters were determined and discussed. The adsorption of the investigated compounds on zinc was found to obey Langmuir adsorption isotherm.