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Neutron Diffraction Study of Phase Stresses in Al/SiCp Composite During Tensile Test
Elżbieta Gadalińska,Andrzej Baczmański,Sebastian Wroński,Przemysław Kot,Marcin Wroński,Mirosław Wróbel,Christian Scheffzük,Gizo Bokuchava,Krzysztof Wierzbanowski 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.3
The stress partitioning between phases, phase stress relaxation as well as origins of Al/SiCp composite strengthening arestudied in the present work. In this aim, the measurements of lattice strains by neutron diffraction were performed in situ duringtensile test up to sample fracture. The experimental results were compared with results of elastic–plastic self-consistentmodel. It was found that thermal origin phase stresses relax at the beginning of plastic deformation of Al/SiCp composite. The evolution of lattice strains in both phases can be correctly simulated by the elastic–plastic self-consistent model onlyif the relaxation of initial stresses is taken into account. A major role in the strengthening of the studied composite plays atransfer of stresses to the SiCpreinforcement, however the hardness of Al metal matrix is also important.
Confinement of concrete in two-chord battened composite columns
Elżbieta Szmigiera 국제구조공학회 2015 Steel and Composite Structures, An International J Vol.19 No.6
This article provides an analysis of the complex character of stress distribution in concrete in stub columns consisting of two HE160A steel sections held together with batten plates and filled with concrete. In such columns, evaluating the effect of concrete confinement and determining the extent of this confinement constitute a substantially complex problem. The issue was considered in close correspondence to rectangular cross section tubular elements filled with concrete, concrete-encased columns, as well as to steel-concrete columns in which reinforcement bars are connected with shackles. In the analysis of concrete confinement in two-chord columns, elements of computational methods developed for different types of composite cross sections were adopted. The achieved analytical results were compared with calculations based on test results.
NANOTECHNOLOGY IN PHARMACEUTICAL AND BIOMEDICAL APPLICATIONS: DENDRIMERS
PAWEŁ SZYMAŃSKI,MAGDALENA MARKOWICZ,ELŻBIETA MIKICIUK-OLASIK 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2011 NANO Vol.6 No.6
This review focuses on the synthesis, biological and pharmacokinetic properties of dendrimers, a relatively new class of nanoparticles. Furthermore, it deals with a wide variety of applications of these molecules, mainly concentrating on their potential use as nanocontainers for controlled delivery of pharmaceutically active compounds. This article also deliberates on dendrimers� development and commercial use in various life science applications.
Aneta Olek,Leszek Klimek,Elżbieta Bołtacz-Rzepkowska 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.6
Background: Bovine and porcine teeth are often used in in vitro experiments as substitutes of human teeth. Objectives: The aim of the present study was to perform a comparative analysis of enamel morphology of permanent human, bovine and porcine teeth under the scanning electron microscope. Methods: As many as 10 human, 10 bovine, and 10 porcine teeth were studied. All the teeth were sectioned and the halves were randomly divided into 2 groups according to the examined tissue (vestibular enamel at the mid-height of the dental crown and in the cervical area). Human and bovine enamel was etched for 15 sec and porcine enamel for 30 sec. The scanning electron microscope analysis was performed. The length and width of enamel prisms were determined with the “Met-Ilo” 1.1 computer program. Results: All enamel samples revealed the same etching pattern—Silverstone's type 2. Bovine enamel showed a similar porosity and the amount of interprismatic enamel compared to human enamel while the amount and width of interprismatic enamel bands in porcine enamel were evidently greater. The shape of the porcine prisms was visually similar to human prisms, although dimensions were significantly different. However, bovine prisms differed in form and appeared to be distinctly elongated. Conclusions: Reported findings indicate that the results of experimental studies carried out on bovine and porcine enamel should not be compared with the results obtained on human enamel.
Application of chloroplast promoters of Cyanidioschyzon merolae for exogenous protein expression
Tomasz Krupnik,Wioleta Wasilewska,Anna Drożak,Elżbieta Romanowska,Maksymilian Zienkiewicz 한국조류학회I 2018 ALGAE Vol.33 No.4
The ability to transform the chloroplast of Cyanidioschyzon merolae was limited by lack of confirmed and reliablepromoter sequences (among other reasons), capable of delivering stable or modulated DNA transcription followed byprotein synthesis. Our research has confirmed the applicability of three selected chloroplast promoters in C. merolaechloroplast overexpression of the exogenous protein (i.e., chloramphenicol acetyltransferase) and genetic transformation. These results might facilitate further research on genetically modified strains of C. merolae to envisage yet unknownaspect of cellular and plastic physiology as well as C. merolae potential applications as bio-factories or sources of usefulchemicals.
Wypij Magdalena,Ostrowski Maciej,Piska Kamil,Wójcik-Pszczoła Katarzyna,Pękala Elżbieta,Rai Mahendra,Golińska Patrycja 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.9
Silver nanoparticles (AgNPs) have potential applications in medicine, photocatalysis, agriculture, and cosmetic fields due to their unique physicochemical properties and strong antimicrobial activity. Here, AgNPs were synthesized using actinobacterial SL19 strain, isolated from acidic forest soil in Poland, and confirmed by UV-vis and FTIR spectroscopy, TEM, and zeta potential analysis. The AgNPs were polydispersed, stable, spherical, and small, with an average size of 23 nm. The FTIR study revealed the presence of bonds characteristic of proteins that cover nanoparticles. These proteins were then studied by using liquid chromatography with tandem mass spectrometry (LCMS/ MS) and identified with the highest similarity to hypothetical protein and porin with molecular masses equal to 41 and 38 kDa, respectively. Our AgNPs exhibited remarkable antibacterial activity against Escherichia coli and Pseudomonas aeruginosa. The combined, synergistic action of these synthesized AgNPs with commercial antibiotics (ampicillin, kanamycin, streptomycin, and tetracycline) enabled dose reductions in both components and increased their antimicrobial efficacy, especially in the case of streptomycin and tetracycline. Furthermore, the in vitro activity of the AgNPs on human cancer cell lines (MCF-7, A375, A549, and HepG2) showed cancer-specific sensitivity, while the genotoxic activity was evaluated by Ames assay, which revealed a lack of mutagenicity on the part of nanoparticles in Salmonella Typhimurium TA98 strain. We also studied the impact of the AgNPs on the catalytic and photocatalytic degradation of methyl orange (MO). The decomposition of MO was observed by a decrease in intensity of absorbance within time. The results of our study proved the easy, fast, and efficient synthesis of AgNPs using acidophilic actinomycete SL19 strain and demonstrated the remarkable potential of these AgNPs as anticancer and antibacterial agents. However, the properties and activity of such particles can vary by biosynthesized batch.
Roman M. Janas,Anna Rybak,Aldona Wierzbicka-Rucińska,Piotr Socha,Rafał Śnitko,Anna Szaflarska-Popławska,Anna Stolarczyk,Beata Oralewska,Elżbieta Cytra-Jarocka,Barbara Iwańczak,Urszula Grzybowska-Chleb 거트앤리버 소화기연관학회협의회 2016 Gut and Liver Vol.10 No.4
Background/Aims: The roles of the many bioactive peptides in the pathogenesis of celiac disease remain unclear. To evaluate the serum concentrations of insulin, ghrelin, adiponectin, leptin, leptin receptor, and lipocalin-2 in children with celiac disease who do and do not adhere to a gluten-free diet (GFD, intermittent adherence). Methods: Prepubertal, pubertal, and adolescent celiac children were included in this study (74 girls and 53 boys on a GFD and 80 girls and 40 boys off of a GFD). Results: Insulin levels in prepubertal (9.01±4.43 μIU/mL), pubertal (10.3±3.62 μIU/mL), and adolescent (10.8±4.73 μIU/mL) girls were higher than those in boys (5.88±2.02, 8.81±2.88, and 8.81±2.26 μIU/mL, respectively) and were neither age-dependent nor influenced by a GFD. Prepubertal children off of a GFD exhibited higher ghrelin levels than prepubertal children on a GFD. Adiponectin levels were not age-, sex- nor GFD-dependent. Adherence to a GFD had no effect on the expression of leptin, leptin receptor, and lipocalin-2. Conclusions: Adherence to a GFD had no influence on the adiponectin, leptin, leptin receptor, and lipocalin-2 concentrations in celiac children, but a GFD decreased highly elevated ghrelin levels in prepubertal children. Further studies are required to determine whether increased insulin concentrations in girls with celiac disease is suggestive of an increased risk for hyperinsulinemia.